Characterization of bacterial isolates from water reclamation systems on the basis of substrate utilization patterns and regrowth potential in reclaimed water

2013 ◽  
Vol 68 (7) ◽  
pp. 1556-1565 ◽  
Author(s):  
Parinda Thayanukul ◽  
Futoshi Kurisu ◽  
Ikuro Kasuga ◽  
Hiroaki Furumai
Keyword(s):  
Amino Acids ◽  
Water Reuse ◽  
Reclaimed Water ◽  
Substrate Utilization ◽  
Water Reclamation ◽  
Operational Taxonomic Units ◽  
Utilization Patterns ◽  
Microbial Regrowth ◽  
Similar Growth

Microbial regrowth causes problems during water reuse. Comprehensive understanding of the microorganisms that can regrow in reclaimed water and their substrate requirements are necessary. In this study, potential regrowth organisms were isolated from seven water reclamation plants in Japan. Based on 16S rDNA analysis, the isolates were grouped into 34 operational taxonomic units, belonging to Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Substrate utilization profiling using Biolog microplate™ classified the isolates into four groups. Bacteria in Cluster 1 (e.g., Methylobacterium sp. and Acinetobacter sp.) mainly utilized polymers, esters, amides, and alcohol. Isolates in Cluster 2 (e.g., Flavobacterium sp. and Microbacterium sp.) preferred to utilize polymers, carbohydrates, and esters. Isolates in Cluster 3 (e.g., Pseudomonas sp. and Acidovorax sp.) mainly utilized esters, carboxylic acids, and amino acids. Isolates in Cluster 4 (e.g., Enterobacter sp. and Rhodococcus sp.) utilized carbohydrates, esters, and amino acids. All isolates grew in reclaimed water treated by sand filtration, whereas some isolates could not grow in reclaimed water treated by coagulation and ozonation. Most bacteria in the same Biolog clusters exhibited similar growth characteristics in water samples. The potential of bacteria to regrow in reclaimed water likely depended on substrate requirement.

Water reclamation and reuse criteria in the U.S.

1996 ◽  
Vol 33 (10-11) ◽  
pp. 451-462 ◽  
Author(s):  
James Crook ◽  
Rao Y. Surampalli
Keyword(s):  
Surface Waters ◽  
Water Reuse ◽  
Reclaimed Water ◽  
Water Reclamation ◽  
Water Supplies ◽  
The Us ◽  
Attractive Option ◽  
Us Epa ◽  
The Individual ◽  
The U.S

Increasing demands on water resources for domestic, commercial, industrial, and agricultural purposes have made water reclamation and reuse an attractive option for conserving and extending available water supplies. Also, many water reuse projects are implemented to eliminate a source of contamination in surface waters or as a least-cost alternative to meeting stringent discharge requirements. Reclaimed water applications range from pasture irrigation to augmentation of potable water supplies. Water reclamation and reuse criteria are principally directed at health protection. There are no federal regulations governing water reuse in the U.S.; hence, the regulatory burden rests with the individual states. This has resulted in differing standards among states that have developed criteria. This paper summarizes and compares the criteria from some states that have developed comprehensive regulations. Guidelines published by the US. EPA and the rationale behind them are presented for numerous types of reclaimed water applications.

Prospects, problems and pitfalls of urban water reuse: a case study

2001 ◽  
Vol 43 (10) ◽  
pp. 9-16 ◽  
Author(s):  
S. W. Hermanowicz ◽  
E. Sanchez Diaz ◽  
J. Coe
Keyword(s):  
San Francisco ◽  
Distribution System ◽  
Water Reuse ◽  
Energy Use ◽  
Large Fraction ◽  
Reclaimed Water ◽  
Treatment Plant ◽  
Water Reclamation ◽  
Bay Area ◽  
Local Water

This paper presents a successful water reclamation and reuse project in the San Francisco Bay area. The project, which includes a water reclamation facility and a separate distribution system, is operated by a wastewater utility and reclaims approximately 4% of its dry-weather flow. Project history, its design and implementation are further discussed. Planning, and especially demand analysis, was critical for project development. Earlier attempts of water reuse were not successful because reclaimed water quality did not match the requirements of potential large industrial customers. Current customers are a mix of public, commercial and residential users who apply the reclaimed water solely for landscape irrigation. In addition, a large fraction of the reclaimed water is used internally in the main wastewater treatment plant. Early connection of largest customers, innovative collaboration with a neighboring reclamation project and cooperation of the local water supplier were very important for project success. Distribution of internal process water consumes most energy. The second major energy use is for the treatment of reclaimed water while distribution of reclaimed water to external customers requires least energy.

Water from (waste)water – the dependable water resource (The 2001 Stockholm Water Prize Laureate Lecture)

2002 ◽  
Vol 45 (8) ◽  
pp. 23-33 ◽  
Author(s):  
Takashi Asano
Keyword(s):  
Water Resources ◽  
Water Supply ◽  
Water Resource ◽  
Water Reuse ◽  
Reclaimed Water ◽  
Treatment Plant ◽  
Quality Criteria ◽  
Water Quality Criteria ◽  
Water Reclamation ◽  
Dependable Water

Water reclamation and reuse provides a unique and viable opportunity to augment traditional water supplies. As a multi-disciplined and important element of water resources development and management, water reuse can help to close the loop between water supply and wastewater disposal. Effective water reuse requires integration of water and reclaimed water supply functions. The successful development of this dependable water resource depends upon close examination and synthesis of elements from infrastructure and facilities planning, wastewater treatment plant siting, treatment process reliability, economic and financial analyses, and water utility management. In this paper, fundamental concepts of water reuse are discussed including definitions, historical developments, the role of water recycling in the hydrologic cycle, categories of water reuse, water quality criteria and regulatory requirements, and technological innovations for the safe use of reclaimed water. The paper emphasizes the integration of this alternative water supply into water resources planning, and the emergence of modern water reclamation and reuse practices from wastewater to reclaimed water to repurified water.

Reclaimed water for the Tarragona petrochemical park

2014 ◽  
Vol 15 (2) ◽  
pp. 308-316 ◽  
Author(s):  
J. Sanz ◽  
J. Suescun ◽  
J. Molist ◽  
F. Rubio ◽  
R. Mujeriego ◽  
...  
Keyword(s):  
Surface Water ◽  
Water Supply ◽  
Water Reuse ◽  
Reclaimed Water ◽  
Cooling Water ◽  
Phase Iii ◽  
Secondary Effluent ◽  
Water Reclamation ◽  
Supply Source ◽  
Start Up

The Camp de Tarragona Water Reuse Project is an emblematic example of how regional water scarcity can be overcome by considering reclaimed secondary effluent, which would otherwise be disposed of in the Mediterranean Sea, as an essential component of integrated water resources management. An advanced water reclamation plant (AWRP) was completed in 2011 to reclaim municipal secondary effluent from Tarragona and Vilaseca-Salou wastewater treatment plants. The reclaimed effluent is used for cooling and process water at the nearby Tarragona petrochemical park. The AWRP's current (2014) capacity is 19,000 m3/d (Phase I), and further expansions are planned to produce 29,000 m3/d (Phase II) and 55,000 m3/d (Phase III) in coming years. This locally available additional water supply will replace surface water supplies currently transferred from the Ebro River for use at the petrochemical park; as a result, an equivalent volume of surface water will be available for urban water supply in the coastal areas of Tarragona province. By developing this new and locally available water supply source, industrial growth in a water scarce region has been supported, while promoting local industry's sustainability. This industrial water reuse project provided 0.20 hm3 of water from September to December 2012, its first operational year, and 1.37 hm3 in 2013. The paper presents and discusses the planning, design, construction and operation phases of this water reclamation and reuse project, including start-up and commissioning, facilities preservation protocols from construction completion to servicing start-up, and the operational, management and economic arrangements adopted to provide a reliable source of reclaimed water for cooling water systems and demineralized water for boiler feed at the Tarragona petrochemical park and a nearby cogeneration power plant.

Water reclamation and intersectoral water transfer between agriculture and cities – a FAO economic wastewater study

2011 ◽  
Vol 63 (5) ◽  
pp. 1067-1073 ◽  
Author(s):  
Ingo Heinz ◽  
Miquel Salgot ◽  
Sasha Koo-Oshima
Keyword(s):  
Water Resources ◽  
Water Reuse ◽  
Water Exchange ◽  
Reclaimed Water ◽  
Cost Savings ◽  
Cost Benefit ◽  
Economic Incentives ◽  
Water Transfer ◽  
Environmental Benefits ◽  
Water Reclamation

Cost–benefit studies on replacing conventional agricultural water resources with reclaimed water in favour of cities are still rare. Some results of a study under auspices of the Food and Agriculture Organisation (FAO) are presented. By means of an illustrative example at Lobregat River basin in Spain, it could be proved that reclaimed water reuse and intersectoral water transfer can result in economic and environmental benefits at the watershed level. The agricultural community faces cost savings in water pumping and fertilising, increases in yields and incomes; the municipality benefits from additional water resources released by farmers. Farmers should be encouraged to participate by implementing adequate economic incentives. Charging farmers with the full cost of water reclamation may discourage farmers from joining water exchange projects. Particularly in regions with water scarcity, investments in reclaimed water reuse and water exchange arrangements usually pay back and are profitable in the long term.

Post-proline endopeptidase, further characterization of the enzyme from pig kidneys

1984 ◽  
Vol 49 (8) ◽  
pp. 1846-1853 ◽  
Author(s):  
Karel Hauzer ◽  
Tomislav Barth ◽  
Linda Servítová ◽  
Karel Jošt
Keyword(s):  
Amino Acids ◽  
Aspartic Acid ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Relative Molecular Mass ◽  
Enzyme Molecule ◽  
Thiol Compounds ◽  
Modified Method ◽  
N Terminus

A post-proline endopeptidase (EC 3.4.21.26) was isolated from pig kidneys using a modified method described earlier. The enzyme was further purified by ion exchange chromatography on DEAE-Sephacel. The final product contained about 95% of post-proline endopeptidase. The enzyme molecule consisted of one peptide chain with a relative molecular mass of 65 600 to 70 000, containing a large proportion of acidic and alifatic amino acids (glutamic acid, aspartic acid and leucine) and the N-terminus was formed by aspartic acid or asparagine. In order to prevent losses of enzyme activity, thiol compounds has to be added.

scholarly journals New Insights on the Zeugodacus cucurbitae (Coquillett) Bacteriome

2021 ◽  
Vol 9 (3) ◽  
pp. 659
Author(s):  
Elias Asimakis ◽  
Panagiota Stathopoulou ◽  
Apostolis Sapounas ◽  
Kanjana Khaeso ◽  
Costas Batargias ◽  
...  
Keyword(s):  
Relative Abundance ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Mutual Exclusion ◽  
Bacterial Composition ◽  
Operational Taxonomic Units ◽  
Host Diet ◽  
Dominant Bacteria ◽  
High Degree

Various factors, including the insect host, diet, and surrounding ecosystem can shape the structure of the bacterial communities of insects. We have employed next generation, high-throughput sequencing of the 16S rRNA to characterize the bacteriome of wild Zeugodacus (Bactrocera) cucurbitae (Coquillett) flies from three regions of Bangladesh. The tested populations developed distinct bacterial communities with differences in bacterial composition, suggesting that geography has an impact on the fly bacteriome. The dominant bacteria belonged to the families Enterobacteriaceae, Dysgomonadaceae and Orbaceae, with the genera Dysgonomonas, Orbus and Citrobacter showing the highest relative abundance across populations. Network analysis indicated variable interactions between operational taxonomic units (OTUs), with cases of mutual exclusion and copresence. Certain bacterial genera with high relative abundance were also characterized by a high degree of interactions. Interestingly, genera with a low relative abundance like Shimwellia, Gilliamella, and Chishuiella were among those that showed abundant interactions, suggesting that they are also important components of the bacterial community. Such knowledge could help us identify ideal wild populations for domestication in the context of the sterile insect technique or similar biotechnological methods. Further characterization of this bacterial diversity with transcriptomic and metabolic approaches, could also reveal their specific role in Z. cucurbitae physiology.

scholarly journals Avoided motifs: short amino acid strings missing from protein datasets

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Pablo Mier ◽  
Miguel A. Andrade-Navarro
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Function ◽  
Large Protein ◽  
New Approach ◽  
Cellular Context ◽  
Human Proteins ◽  
Context Specific ◽  
Protein Datasets

Abstract According to the amino acid composition of natural proteins, it could be expected that all possible sequences of three or four amino acids will occur at least once in large protein datasets purely by chance. However, in some species or cellular context, specific short amino acid motifs are missing due to unknown reasons. We describe these as Avoided Motifs, short amino acid combinations missing from biological sequences. Here we identify 209 human and 154 bacterial Avoided Motifs of length four amino acids, and discuss their possible functionality according to their presence in other species. Furthermore, we determine two Avoided Motifs of length three amino acids in human proteins specifically located in the cytoplasm, and two more in secreted proteins. Our results support the hypothesis that the characterization of Avoided Motifs in particular contexts can provide us with information about functional motifs, pointing to a new approach in the use of molecular sequences for the discovery of protein function.

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