Nitrogen removal efficiency and microbial community analysis of ANAMMOX biofilter at ambient temperature

2015 ◽  
Vol 71 (5) ◽  
pp. 725-733 ◽  
Author(s):  
Zeng Taotao ◽  
Li Dong ◽  
Zeng Huiping ◽  
Xie Shuibo ◽  
Qiu Wenxin ◽  
...  
Keyword(s):  
Microbial Community ◽  
Ambient Temperature ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Removal Rate ◽  
Ammonia Concentration ◽  
Visual Observation ◽  
Microbial Community Analysis ◽  
Anammox Bacteria

An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3–23.2 °C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m3 day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.

scholarly journals Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1832
Author(s):  
Jie Xu ◽  
Chao Zhu ◽  
Yi Liu ◽  
Guanghui Lv ◽  
Changyan Tian ◽  
...  
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Removal Rate ◽  
Fixed Bed ◽  
Microbial Community Analysis ◽  
Sequencing Analysis ◽  
Fixed Bed Bioreactor

Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.

scholarly journals Nitrogen removal and functional bacteria distribution of ANAMMOX at ambient temperature

2016 ◽  
Vol 6 (4) ◽  
pp. 476-483 ◽  
Author(s):  
Taotao Zeng ◽  
Dong Li ◽  
Wei Liao ◽  
Wenxin Qiu ◽  
Jie Zhang
Keyword(s):  
Ambient Temperature ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Removal Rate ◽  
Rhodopseudomonas Palustris ◽  
Middle Part ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Ambient Temperatures ◽  
Gradient Gel Electrophoresis

In this study, an up-flow anaerobic biofilter (AF) was operated to investigate the efficiency of anaerobic ammonium oxidation (ANAMMOX) in treating low strength ammonia (46.5 mg/L) at ambient temperatures (20.3–23.2 °C). Microbial compositions and functional populations of the upper (140–190 cm), middle (40–140 cm), and lower (0–40 cm) parts of the biofilter were monitored using scanning electron microscopy, denaturing gradient gel electrophoresis (DGGE), clone and sequence. The results show that stable biofilter performance was achieved with an average nitrogen removal rate of 2.26 kg/(m3·d) and a total nitrogen removal efficiency of 75.9%. Approximately 67% of the ammonia and nitrite disappeared in the middle part of the biofilter. The spherical bacteria, similar to ANAMMOX bacteria, dominated the middle part of the biofilter. There were eight bacterial DGGE bands; clone and sequence results showed that they included Oxalicibacterium sp., Ignavibacterium album, Bacterium rJ15, Candidatus Kuenenia stuttgartiensis, Hippea maritima, Thioprofundum lithotrophica, and Rhodopseudomonas palustris. The genus of ANAMMOX bacterium remaining at constant levels in different parts of the biofilter was identified as Candidatus Kuenenia stuttgartiensis. The AF bioreactor maintained high activity due to the ANAMMOX bacteria's ability to adapt to ambient temperature and low matrix influent conditions.

scholarly journals Achieving fast start-up of anammox process by promoting the growth of anammox bacteria with FeS addition

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Chunzhen Zou ◽  
Beibei Guo ◽  
Xuming Zhuang ◽  
Liying Ren ◽  
Shou-Qing Ni ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Nitrite Reductase ◽  
Removal Rate ◽  
Anammox Bacteria ◽  
Gene Encoding ◽  
Start Up ◽  
Fast Start ◽  
The Difference ◽  
Anammox Process

Abstract The effects of FeS on nitrogen removal performance and microbial community of anammox process were studied. During the start-up period, the removal efficiencies of nitrite and total nitrogen were significantly improved by FeS. The addition of FeS increased the content of iron ions in the reactor and promoted the synthesis of heme c, which was involved in the formation of various enzymes. Compared with the control, the abundance of anammox bacteria in the FeS reactor was increased by 29%, and the expression level of the nirS gene (encoding cd1 type nitrite reductase containing heme) was nearly doubled. The content of nitrite reductase (ammonia-forming) in the community was increased by 26.4%. The difference in functional bacteria and enzyme contents in the microbial community resulted in a difference in nitrogen removal rate (NRR) between the two reactors. High-throughput results indicated that FeS increased the richness and diversity of microbial community and enhanced the metabolic function of the microbial community. The addition of FeS did not change the dominant position of Ca. Kuenenia in both reactors. But the relative abundance of heterotrophic denitrifying bacteria was reduced with FeS, which may be related to the inhibition effect of S2− produced by FeS.

Characterization of microbial community in nitrogen removal process of metallurgic wastewater by PCR-DGGE

2002 ◽  
Vol 46 (11-12) ◽  
pp. 93-98 ◽  
Author(s):  
S. Yoshie ◽  
N. Noda ◽  
T. Miyano ◽  
S. Tsuneda ◽  
A. Hirata ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Precious Metals ◽  
Community Analysis ◽  
Packed Bed ◽  
Community Diversity ◽  
Industrial Wastes ◽  
Microbial Community Analysis ◽  
Gradient Gel Electrophoresis

The metallurgic wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of nitrogen compounds such as ammonia and nitric acid and of salts such as sodium chloride and sodium sulfate. Biological nitrogen removal from this wastewater was attempted by a circulating bioreactor system equipped with an anoxic packed bed and an aerobic fluidized bed. The anoxic packed bed of this system was found to effectively remove nitrite and nitrate from the wastewater by denitrification at a removal ratio of 97%. As a result of denitrification activity tests at various NaCl concentrations, the sludge obtained from the anoxic packed bed exhibited accumulation of nitrite at 5.0 and 8.4% NaCl concentrations, suggesting that the reduction of nitrite is the key step in the denitrification pathway under hypersaline conditions. The microbial community analysis by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA (rDNA) fragments revealed that the community diversity varied in accordance with water temperature, nitrate-loading rate and ionic strength. When particular major DGGE bands were excised, reamplified and directly sequenced, the dominant species in the anoxic packed bed were affiliated with the beta and gamma subclasses of the class Proteobacteria such as Alcaligenes defragrans and Pseudomonas spp., respectively.

Enrichment of marine anammox bacteria from seawater-related samples and bacterial community study

2010 ◽  
Vol 61 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Y. Kawagoshi ◽  
Y. Nakamura ◽  
H. Kawashima ◽  
K. Fujisaki ◽  
K. Furukawa ◽  
...  
Keyword(s):  
Waste Disposal ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Enrichment Culture ◽  
Removal Rate ◽  
Nitrogen Loading ◽  
Disposal Site ◽  
Anammox Bacteria ◽  
Ammonium Oxidation ◽  
Waste Disposal Site

Anaerobic ammonium oxidation (anammox) is a novel nitrogen pathway catalyzed by anammox bacteria which are obligate anaerobic chemoautotrophs. In this study, enrichment culture of marine anammox bacteria (MAAOB) from the samples related to seawater was conducted. Simultaneous removal of ammonium and nitrite was confirmed in continuous culture inoculated with sediment of a sea-based waste disposal site within 50 days. However, no simultaneous nitrogen removal was observed in cultures inoculated with seawater-acclimated denitrifying sludge or with muddy sediment of tideland even during 200 days. Nitrogen removal rate of 0.13 kg/m3/day was achieved at nitrogen loading rate of 0.16 kg/m3/day after 320th days in the culture inoculated with the sediment of waste disposal site. The nitrogen removal ratio between ammonium nitrogen and nitrite nitrogen was 1:1.07. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that an abundance of the bacteria close to MAAOB and coexistence of ammonium oxidizing bacteria and denitrifying bacteria in the culture.

Study on De-Nitrification of Improved Step-Feed Progress

2014 ◽  
Vol 703 ◽  
pp. 171-174
Author(s):  
Bing Wang ◽  
Yi Xiao ◽  
Shou Hui Tong ◽  
Lan Fang ◽  
Da Hai You ◽  
...  
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Fluidized Bed ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Operating Conditions ◽  
Removal Mechanism ◽  
Aerobic Zone

Improved step-feed de-nitrification progress combined with biological fluidized bed was introduced in this study. The progress had good performance and capacity of de-nitrification and organic matter. The experiment result showed that the de-nitrification efficiency of the improved biological fluidized bed with step-feed process was higher than the fluidized bed A/O process under the same water quality and the operating conditions. When the influent proportion of each segment was equal, the system showed good nitrogen removal efficiency with the change of influent C/N ratio, HRT and sludge return ratio. The removal rate of TN reached up to 88.2%. It showed that the simultaneous nitrification and de-nitrification phenomenon happened in the aerobic zone. The nitrogen removal mechanism was also studied.

scholarly journals Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Packed Bed ◽  
Municipal Wastewater Treatment ◽  
Two Stage ◽  
N Removal ◽  
Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

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