scholarly journals Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1832
Author(s):  
Jie Xu ◽  
Chao Zhu ◽  
Yi Liu ◽  
Guanghui Lv ◽  
Changyan Tian ◽  
...  
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Removal Rate ◽  
Fixed Bed ◽  
Microbial Community Analysis ◽  
Sequencing Analysis ◽  
Fixed Bed Bioreactor

Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.

Nitrogen removal efficiency and microbial community analysis of ANAMMOX biofilter at ambient temperature

2015 ◽  
Vol 71 (5) ◽  
pp. 725-733 ◽  
Author(s):  
Zeng Taotao ◽  
Li Dong ◽  
Zeng Huiping ◽  
Xie Shuibo ◽  
Qiu Wenxin ◽  
...  
Keyword(s):  
Microbial Community ◽  
Ambient Temperature ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Removal Rate ◽  
Ammonia Concentration ◽  
Visual Observation ◽  
Microbial Community Analysis ◽  
Anammox Bacteria

An upflow anaerobic biofilter (AF) was developed to investigate anaerobic ammonium-oxidizing (ANAMMOX) efficiency in treating low-strength wastewater at ambient temperature (15.3–23.2 °C). Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization were used to investigate treatment effects on the microbial community. Stepwise decreases in influent ammonia concentration could help ANAMMOX bacteria selectively acclimate to low-ammonia conditions. With an influent ammonia concentration of 46.5 mg/L, the AF reactor obtained an average nitrogen removal rate of 2.26 kg/(m3 day), and a removal efficiency of 75.9%. polymerase chain reaction-DGGE results showed that microbial diversity in the low matrix was greater than in the high matrix. Microbial community structures changed when the influent ammonia concentration decreased. The genus of functional ANAMMOX bacteria was Candidatus Kuenenia stuttgartiensis, which remained stationary across study phases. Visual observation revealed that the relative proportions of ANAMMOX bacteria decreased from 41.6 to 36.3% across three study phases. The AF bioreactor successfully maintained high activity due to the ANAMMOX bacteria adaptation to low temperature and substrate conditions.

scholarly journals Nitrogen Removal by HN-AD Bacteria Immobilized on Modified Absorbent Stone

2020 ◽  
Vol 34 (3) ◽  
pp. 193-207
Author(s):  
Wen Zhang ◽  
Zhen Zhang ◽  
Sufeng Wang
Keyword(s):  
Removal Efficiency ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
High Efficiency ◽  
Removal Rate ◽  
Low Cost ◽  
Ammonia Nitrogen ◽  
Aerobic Denitrification ◽  
Immobilized Microorganisms ◽  
The Cost

How to simplify the nitrogen removal process, reduce the cost and improve the efficiency has become an urgent problem to be solved. In this research, the isolated HNAD (heterotrophic nitrification and aerobic denitrification) bacteria were used to remove<br /> the nitrogen in wastewater. Modified absorbent stone was used as high-efficiency and<br /> low-cost immobilized material. The modification effect was determined by the changes<br /> in mechanical strength, Zeta potential, pore structure, micrographs and biomass. The<br /> practicability of the modified carrier was further proved by experiments of environmental effect and reuse. The modified carrier had excellent performance. By comparing the<br /> degradation effects of immobilized microorganism and free microorganism, it was proved<br /> that the immobilized microorganisms have broad application prospects and strong adaptability to environmental factors. Under the optimum conditions (temperature of 30 oC,<br /> pH of 7, dissolved oxygen of 3.5 mg L–1), the removal efficiency of ammonia nitrogen<br /> reached 100 % in 40 hours, the removal efficiency of total nitrogen reached 60.11 % in<br /> 50 hours, and the removal rate of total nitrogen was 2.404 mg-NL–1 h–1 by immobilized<br /> microorganisms with the treatment of simulated nitrogen-containing wastewater. This<br /> research provides new material for the immobilization of HN-AD bacteria and a new way for nitrogen removal.

scholarly journals Microbial community with the ability to biodegradation perchlorate in a bio-electrochemical reactor

2019 ◽  
Vol 118 ◽  
pp. 01021
Author(s):  
Yu Zhong ◽  
Li He ◽  
Guangyi Fu ◽  
Youze Xu ◽  
Lixia Xie ◽  
...  
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Electrochemical Reactor ◽  
Hunan Province ◽  
Sequencing Analysis ◽  
Initial Concentration ◽  
Perchlorate Reduction ◽  
Class Level

In this study, the sediments from the Liuyang River (Hunan Province, China) were used as an inoculum to bio-reduction perchlorate in a bio-electrochemical reactor (BER). The efficient degradation of perchlorate was found in the BER by utilizing the hydrogen as electron donor. When the current intensity was 10 mA and HRT was 72 h, the removal rate of perchlorate (initial concentration was 5 mg/L) reached 84.13% and a removal flux of 178.68 mg/m2·d was achieved. High-throughput sequencing analysis confirmed that the biofilm in the reactor had been successfully acclimated, and the system could achieve perchlorate reduction effectively. Firmicutes and Bacteroidetes were the dominant phyla during inoculation phase, and Actinobacteriria, Proteobacteria, and Tenericutes also constituted a low proportion in the biofilm. Bacilli and Clostridia were dominant at class-level both in inoculum and biofilm, with the relative abundance about 56%-72% and 17%-23%, respectively. These results confirmed that the biofilm in the BER system had been successfully formed, and the BER system could biodegradation perchlorate effectively.

scholarly journals Effect of organic concentration on biological activity and nitrogen removal performance in anammox biofilm system

2021 ◽  
Author(s):  
Weide Fu ◽  
Rencheng Zhu ◽  
Huanyun Lin ◽  
Yukai Zheng ◽  
Zhanbo Hu
Keyword(s):  
Organic Matter ◽  
Biological Activity ◽  
Removal Efficiency ◽  
Relative Abundance ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Ammonia Nitrogen ◽  
Anammox Bacteria ◽  
Sequencing Analysis ◽  
Ammonium Oxidation

Abstract The effects of different concentrations of organic matter on the biological activity and nitrogen removal performance of the anaerobic ammonium oxidation (anammox) system was studied. The results showed that under the condition of low influent total organic carbon (TOC ≤ 100 mg/L), the activity rate of anammox bacteria was basically unaffected as well as the anammox bacteria and denitrifying bacteria formed a good synergistic effect, and the maximum the total nitrogen (TN) removal efficiency reached 95.77%. However, When the influent TOC concentration was up to 200 mg/L, the activity of anammox bacteria was seriously inhibited. At this time, denitrification becomes the main pathway of nitrogen removal, the effluent ammonia nitrogen content increases, and the TN removal efficiency decreases to 64.17%. High-throughput sequencing analysis showed that with the increase of the organic matter concentration, the relative abundance of Proteobacteria and Planctomycetes changed significantly. In particular, the relative abundance proportion of Proteobacteria increased from 21.06% to 25.57%, the Planctomycetes dropped from 10.01% to 3.03% and the Candidatus Brocadia genus had the largest decrease. In conclusion, the concentration range of organic matter for collaborative denitrification was proposed in this study, which provided theoretical reference for the practical application of anammox biofilm process.

Study on De-Nitrification of Improved Step-Feed Progress

2014 ◽  
Vol 703 ◽  
pp. 171-174
Author(s):  
Bing Wang ◽  
Yi Xiao ◽  
Shou Hui Tong ◽  
Lan Fang ◽  
Da Hai You ◽  
...  
Keyword(s):  
Water Quality ◽  
Organic Matter ◽  
Fluidized Bed ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Removal Rate ◽  
Operating Conditions ◽  
Removal Mechanism ◽  
Aerobic Zone

Improved step-feed de-nitrification progress combined with biological fluidized bed was introduced in this study. The progress had good performance and capacity of de-nitrification and organic matter. The experiment result showed that the de-nitrification efficiency of the improved biological fluidized bed with step-feed process was higher than the fluidized bed A/O process under the same water quality and the operating conditions. When the influent proportion of each segment was equal, the system showed good nitrogen removal efficiency with the change of influent C/N ratio, HRT and sludge return ratio. The removal rate of TN reached up to 88.2%. It showed that the simultaneous nitrification and de-nitrification phenomenon happened in the aerobic zone. The nitrogen removal mechanism was also studied.

scholarly journals In Situ Water Quality Improvement Mechanism (Nitrogen Removal) by Water-Lifting Aerators in a Drinking Water Reservoir

Water ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 1051 ◽  
Author(s):  
Zizhen Zhou ◽  
Tinglin Huang ◽  
Weijin Gong ◽  
Yang Li ◽  
Yue Liu ◽  
...  
Keyword(s):  
Drinking Water ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Water Reservoir ◽  
Control Area ◽  
Nitrite Accumulation ◽  
Field Scale ◽  
Drinking Water Reservoir ◽  
N Removal

A field scale experiment was performed to explore the nitrogen removal performance of the water and surface sediment in a deep canyon-shaped drinking water reservoir by operating WLAs (water-lifting aerators). Nitrogen removal performance was achieved by increasing the densities and N-removal genes (nirK and nirS) of indigenous aerobic denitrifiers. After the operation of WLAs, the total nitrogen removal rate reached 29.1 ± 0.8% in the enhanced area. Ammonia and nitrate concentrations were reduced by 72.5 ± 2.5% and 40.5 ± 2.1%, respectively. No nitrite accumulation was observed. Biolog results showed improvement of carbon metabolism and carbon source utilization of microbes in the enhanced area. Miseq high-throughput sequencing indicated that the denitrifying bacteria percentage was also higher in the enhanced area than that in the control area. Microbial communities had changed between the enhanced and control areas. Thus, nitrogen removal through enhanced indigenous aerobic denitrifiers by the operation of WLAs was feasible and successful at the field scale.

scholarly journals Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Packed Bed ◽  
Municipal Wastewater Treatment ◽  
Two Stage ◽  
N Removal ◽  
Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

Sign in / Sign up

Export Citation Format

Share Document