scholarly journals Unintentional transport of fungi propagules to Antarctic biome and the ability to develop at low temperatures

2020 ◽  
Vol 18 (5) ◽  
pp. 271-281
Author(s):  
Anna Augustyniuk-Kram
Keyword(s):  
Botrytis Cinerea ◽  
Filamentous Fungi ◽  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
Low Temperatures ◽  
Trichoderma Viride ◽  
Geotrichum Candidum ◽  
Cladosporium Cladosporioides ◽  
Freezing And Thawing ◽  
Penicillium Sp

Filamentous fungi relatively easily disperse and colonize a variety of substrates, inhabiting various, often extreme environments. Therefore, they spread all over the world. The purpose of the research was to determine whether the propagules of filamentous fungi brought (accidentally transported) into the Antarctic biome by tourists and members of scientific expeditions are capable of developing at low temperatures. In the studies were used seven isolates of fungi: Penicillium sp., Aspergillus flavus, Alternaria alternata, Cladosporium cladosporioides, Trichoderma viride, Geotrichum candidum and Botrytis cinerea. The isolates came from samples collected from tourists and members of scientific expeditions arriving at the Henryk Arctowski Polish Antarctic Station on King George Island in the South Shetland archipelago. Fungal growth was measured at 0, 5, 10, 22°C (as a control) and 10° C, but after having frozen inoculum at -15°C for a period of 7 days. Penicillium sp., Alternaria alternata, Cladosporium cladosporioides, Trichoderma viride, Geotrichum candidum and Botrytis cinerea were found to be capable of growing at low temperatures (5 and 10oC as well as after one freezing cycle, down to -15oC and thawing, up to +10oC). They did not produce a macroscopically visible mycelium at temp. 0oC, however, it was not a lethal temperature for them, as when they were transferred to higher temperatures, they continued to develop even after a fairly long time following the beginning of the experiment. The most vulnerable was Aspergillus flavus. At lower temperatures (from about to 5oC) it did not develop, while freezing and thawing were lethal for this species. Some species (G. candidum, T. viride and B. cinerea), despite the development of mycelium, did not produce spores at lower temperatures.

scholarly journals Niezamierzony transport propaguli grzybów pleśniowych do biomu Antarktyki a zdolność rozwoju w niskich temperaturach

2016 ◽  
Vol 14 (4) ◽  
pp. 149-166
Author(s):  
Anna Augustyniuk-Kram
Keyword(s):  
Botrytis Cinerea ◽  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
Trichoderma Viride ◽  
Geotrichum Candidum ◽  
Cladosporium Cladosporioides ◽  
Penicillium Sp

Obecność człowieka w Antarktyce to przede wszystkim działalność naukowa, ale również w ostatnim czasie wzmożony ruch turystyczny. Sprzyja to inwazji obcych gatunków flory i fauny, a także mikroorganizmów, mogących zagrażać gatunkom rodzimym. Grzyby pleśniowe będące przedmiotem badań zaliczane są do organizmów kosmopolitycznych, łatwo rozprzestrzeniających się i zasiedlających różnorodne środowiska, w tym również ekstremalnie zimne, takie jak rejony polarne. Organizmy te, by skutecznie skolonizować nowe środowisko oprócz żywotnych propagul i skutecznych mechanizmów transportu muszą być zdolne do wzrostu i reprodukcji w ekstremalnych warunkach. Celem badań było określenie czy propagule grzybów pleśniowych zawleczone (przypadkowo przetransportowane) do biomu Antarktyki przez turystów i członków wypraw naukowych są zdolne do rozwoju w niskich temperaturach. Stwierdzono, że Penicillium sp., Alternaria alternata, Cladosporium cladosporioides, Trichoderma viride, Geotrichum candidum i Botrytis cinerea były zdolne do rozwoju w niskich temperaturach (5 i 10oC oraz po jednym cyklu zamrożenia do -15oC i odmrożenia do +10oC). Nie wytwarzały one makroskopowo widocznej grzybni w temp. 0oC, lecz nie była to dla nich temperatura letalna, ponieważ po przeniesieniu do wyższych temperatur podejmowały wzrost nawet po dosyć długim czasie od rozpoczęcia eksperymentu. Najbardziej wrażliwy okazał się Aspergillus flavus. Przy niższych temperaturach (od 0 do 5oC) nie rozwijał się, natomiast zamrożenie i odmrożenie było dla tego gatunku letalne. Niektóre gatunki (G. candidum, T. viride i B. cinerea) mimo rozwoju grzybni, w niższych temperaturach nie produkowały zarodników.

scholarly journals Fungal diseases of Rose plant in Bangladesh

2014 ◽  
Vol 38 (2) ◽  
pp. 225-233 ◽  
Author(s):  
Anita Ghosh ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
Trichoderma Viride ◽  
Black Spot ◽  
Fungal Diseases ◽  
Rhizopus Stolonifer ◽  
Penicillium Sp ◽  
Botrytis Allii ◽  
Fusarium Sp ◽  
Academy Of Sciences

Five types of symptom were recorded on two varieties of rose plant. The symptoms were Black spot, Leaf spot1, Leaf spot2, Blight and Anthracnose. The study revealed the presence of 20 species of fungi belonging to 17 genera. The isolated fungi were Alternaria alternata (Fr.) Keissler, Arthrinium saccharicola Stevenson, Aspergillus flavus, Link., A. niger van Tiegh., Botrytis allii Munn, Cercospora sp., Cladosporium cladosporioides (Fresen.) de Vries, C. oxysporum Berk. & Curt., two species of Colletotrichum, Curvularia brakyospora Boedijn, Curvularia pallescens Boedijn, Fusarium sp., Epicoccum purpurascens Ehreneb ex Schlecht; Link, Gibberella sp., Marssonina rosea (Lib.) Died, Nigrospora sphaerica (Sacc.) Masson, Pestalotiopsis guepinii (Desm.) Stay. with its two culture types, Penicillium sp., Rhizopus stolonifer (Ehrenb. Ex. Fr) Vuill. and Trichoderma viride Pers. ex Fries. The frequency (%) of association of P. guepinii was higher than any other fungi. Pestalotiopsis guepinii and its two culture types were found to be pathogenic to rose plant. DOI: http://dx.doi.org/10.3329/jbas.v38i2.21347 Journal of Bangladesh Academy of Sciences, Vol. 38, No. 2, 225-233, 2014

scholarly journals Fungi inhabiting healthy grapevine canes (Vitis spp.) in some nurseries

2012 ◽  
Vol 59 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Ewa Król
Keyword(s):  
Botrytis Cinerea ◽  
Alternaria Alternata ◽  
Present Experiment ◽  
Plant Material ◽  
Cladosporium Cladosporioides ◽  
The Other ◽  
Qualitative Composition ◽  
Vitis Spp ◽  
Propagation Material ◽  
Apparently Healthy

The purpose of this study, conducted in the years 2000 - 2002, was to identify fungi species colonizing apparently healthy canes and to investigate whether canes storage modify the quantitative and qualitative composition of these fungi. The plant material was collected from 5 commercial plantations growing in various regions of Poland, taking into consideration 8 cultivars which were the most frequently cultivated. From each plantation and cultivar 20 apparently healthy canes were randomly sampled in two terms: before storage - November/December (term I) and 3-4 months after storage - February/March (term II). The results showed that from asymptomatic canes 2746 isolates of fungi belonging to 23 species were obtained, but the majority of them origined from canes analysed after storage. It was found that <i>P. viticola</i> is able to live latently within grapevine tissue in Polish conditions because isolates of this fungus from visually healty canes the all studied plantations and terms were obtained. Among the other fungi species inhabiting grapevine canes <i>Alternaria alternata</i> and <i>Fusarium</i> spp. dominated. Moreover, both in term I and term II <i>Botrytis cinerea</i>, <i>Phoma</i> spp., <i>Epicoccum purpurascens</i> and <i>Cladosporium cladosporioides</i> were frequently isolated, whereas fungi from the genus <i>Acremonium</i> only in the term I. Each time isolates of <i>Trichoderma</i> spp. and <i>Gliocladium</i> spp. were also obtained. Inhabitation of grapevine canes by various fungi species shown in the present experiment indicate the danger of pathogens spread with propagation material on the new plantations.

scholarly journals Prevalence and Pathogenic Potentiality of Fungi Associated With Leaf Spot of Basella Alba and B. Rubra

2020 ◽  
Vol 29 (2) ◽  
pp. 183-189
Author(s):  
Nusrat Jahan Shova ◽  
Shamim Shamsi ◽  
MA Bashar
Keyword(s):  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
Leaf Spot ◽  
Curvularia Lunata ◽  
Basella Alba ◽  
Penicillium Sp ◽  
Colletotrichum Dematium ◽  
Major Species ◽  
Fusarium Semitectum ◽  
First Time

Two major species of Indian spinach, namely Basella alba L. and B. rubra L. were selected to find out associated mycoflora and their pathogenic potentiality. Ten fungi viz., Alternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, Colletotrichum dematium, C. lindemuthianum, Curvularia lunata, Drechslera sacchari, Fusarium semitectum and Penicillium sp. were found to be associated with the selected leaves of Basella spp. Among the isolated fungi C. lindemuthianum, D. sacchari and F. semitectum were found to be pathogenic to Basella spp. Association of C. lindemuthianum and D. sacchari with Basella spp. are recorded first time from Bangladesh. Dhaka Univ. J. Biol. Sci. 29(2): 183-189, 2020 (July)

scholarly journals Phylloplane mycoflora of Gerbera spp. and their Pathogenic potentiality

2014 ◽  
Vol 37 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Fatema Yeasmin ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Flavus ◽  
New Record ◽  
Trichoderma Viride ◽  
Fungal Species ◽  
Rhizopus Stolonifer ◽  
Penicillium Sp ◽  
Colletotrichum Spp ◽  
Chaetomella Raphigera ◽  
Fusarium Nivale ◽  
Academy Of Sciences

Two types of symptoms, blight and anthracnose, were recorded on two species of Gerbera viz. Gerbera aurantiaca L. and Gerbera viridifolia L. The most common fungal species isolated from Gerbera spp. were Alternaria citrii, A. tennuisssima, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Bipolaris hawaiiensis, Chaetomella raphigera, Cladosporium cladosporoides, Colletotrichum capsici, C. coffeanum, C. dematium, C. gleosporioides, C. lindemuthianum, C. musae, C. orbiculare, Colletorichum sp.1, Colletotrichum sp.2, Colletotrichum sp.3, Colletotrichum sp.4, Curvularia clavata, C. lunata, Fusarium nivale, F. semitectum, Phomopsis sp., Penicillium sp., Pestalotia sp., Rhizopus stolonifer, Trichoderma viride, one cleistothecial ascomycetes and one sterile fungus. The frequency percentage of association of Colletotrichum spp. with Gerbera spp. was higher than any other fungi. Bipolaris hawaiiensis is a new record for Bangladesh. Among the isolated fungi Alternaria citrii, A. tennuissima, Colletotrichum capcisi, C. dematium, C. coffeanum and Curvularia clavata were found to be pathogenic to Gerbera plant. DOI: http://dx.doi.org/10.3329/jbas.v37i2.17562 Journal of Bangladesh Academy of Sciences, Vol. 37, No. 2, 211-217, 2013

scholarly journals Occurrence of pathotypes of Phoma medicaginis var. pinodella (Jones) Boerema in the Olsztyn district

2013 ◽  
Vol 44 (1-2) ◽  
pp. 23-42 ◽  
Author(s):  
Helena Furgał-Węgrzycka
Keyword(s):  
Botrytis Cinerea ◽  
Alternaria Alternata ◽  
Cladosporium Cladosporioides ◽  
Saprophytic Fungi ◽  
Stemphylium Botryosum ◽  
Field Peas ◽  
Phoma Medicaginis ◽  
Microscopic Features ◽  
Sordaria Fimicola ◽  
Mycelia Sterilia

The causal agents of leaves and pods spot-pot of peas and field peas in the Olsztyn district was the fungus <i>Phoma medicaginis</i> var. <i>pinodella</i>. Investigated isolates of <i>P. medicaginis</i> var. <i>pinodella</i> differed by macroscopic and microscopic features. From diseases leaves and pods of peas and field peas obtained also saprophytic fungi among which <i>Alternaria alternata, Botrytis cinerea, Cladosporium cladosporioides, Epicoccum purpurascens, Stemphylium botryosum, Sordaria fimicola</i> and <i>mycelia sterilia</i> were dominated. In the study on the identification and distribution of pathotypes of <i>P. medicaginis</i> var. <i>pinodella</i> six pathotypes were identified among which pathotype 3 were dominated. The identified pathotypes differed by macroscopic and microscopic features.

scholarly journals Toxic Metal Effect on Filamentous Fungi Isolated from the Contaminated Soil of Multan and Gujranwala

2014 ◽  
Vol 1 (2) ◽  
Author(s):  
Anam Rasool ◽  
Shazia Irum
Keyword(s):  
Heavy Metals ◽  
Filamentous Fungi ◽  
Aspergillus Flavus ◽  
Water Samples ◽  
Contaminated Soils ◽  
Metal Tolerance ◽  
Tolerance Index ◽  
Aspergillus Versicolor ◽  
Penicillium Sp ◽  
Soil And Water

Considering the importance of filamentous fungi for bioremediation of wastewater and contaminated soils, this study was planned to investigate the metal tolerance potential of indigenous filamentous fungi. Certain metals are important to biological actions. However all metals, whether essential or inessential will show toxicity at certain levels. During 2012 total 17 fungi were isolated and preserved from contaminated peri-urban agricultural areas of Multan and Gujranwala for further detail investigation of heavy metal tolerance. Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus isolated from both soil and water samples while Aspergillus terreus and Penicillium sp were only isolated from soil samples of Multan and Aspergillus versicolor, Aspergillus flavus, Fusarium oxysporum, Aspergillus niger which were isolated from contaminated soils and water samples while Penicillium sp was isolated from only water samples of Gujranwala. These few fungal isolates were selected for tolerance to metal Cu (SO4)2.5H2O, Cd (NO3)2, Cr (NO3)2 and Pd (NO3)2. The tolerant strains were selected with increasing metals concentration of 100ppm and compared to control in the medium. The degree of tolerance was measured by radial growth (cm) in the presence of various heavy metals and compare to the control, which contain no heavy metals. The present study investigation concludes isolates Penicillium sp and Aspergillus flavus isolated from soil of Gujranwala show maximum tolerance index 2.1 at 100ppm toward Cr and 4.8 at 100ppm toward Cd respectively. Aspergillus Versicolor (isolated from waste water) exhibit considerable highest tolerance index toward Cu and Pb while show a sensitivity against other metals. From all the collected samples the Gujranwala soil and water show more tolerance toward the heavy metals as compared to Multan area.The present study indicates that in future similar strains will be tested with other heavy metals for the confirmation of tolerance and tolerant strains will be used for bioremediation of heavy metal.

scholarly journals Fungi associated with anthracnose of mango (Mangifera indica L.) fruits and their pathogenic potentiality

2018 ◽  
Vol 27 (1) ◽  
pp. 93-100
Author(s):  
Farhana Islam ◽  
Shamim Shamsi ◽  
Md Abul Bashar
Keyword(s):  
Aspergillus Flavus ◽  
Alternaria Alternata ◽  
New Record ◽  
Colletotrichum Gloeosporioides ◽  
Mangifera Indica ◽  
Fungal Species ◽  
Rhizopus Stolonifer ◽  
Penicillium Sp ◽  
Fusarium Semitectum

A total of ten fungal species were isolated from the anthracnose symptoms of three varieties of Mangifera indica L. viz., Himsagar, Langra and Amrapali. The isolated fungi were Alternaria alternata (Fr.) Keissler, Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tiegh., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium semitectum Berk. & Rav., Papulaspora sp. Preuss., Penicillium sp. Link., Pestalotiopsis guepinii (Desm.) Stey. and Rhizopus stolonifer (Ehrenb.) Vull. Colletotrichum gloeosporioides showed the highest per cent (26.82) frequency in Himsagar whereas R. stolonifer showed the lowest (2.22). Pestalotiopsis guepinii showed the highest per cent (46.83) frequency in Langra whereas R. stolonifer showed the lowest (1.17). Colletotrichum gloeosporioides showed the highest per cent (38.67) frequency in Amrapali whereas Papulaspora sp. showed the lowest (4.00). Among the isolated fungi C. gloeosporioides, F. semitectum and P. guepinii were found to be pathogenic to three mango varieties. Association of Fusarium semitectum, Papulaspora sp. and Pestalotiopsis guepinii with mango is new record in Bangladesh. Dhaka Univ. J. Biol. Sci. 27(1): 93-100, 2018 (January)

scholarly journals Perubahan Golongan Darah Berdasarkan Pengaruh Waktu dan Mikroorganisme Yang Berperan

2019 ◽  
Vol 6 (2) ◽  
pp. 165
Author(s):  
Muhamad Masyrur ◽  
I Ketut Junitha ◽  
Meitini W. Proborini
Keyword(s):  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Alternaria Alternata ◽  
Bacillus Coagulans ◽  
Bacillus Sp ◽  
Penicillium Sp ◽  
Fusarium Sp ◽  
Staphylococcus Cohnii ◽  
Antigen A

Darah menjadi salah satu barang bukti yang sangat penting dalam analisa forensik. Darah memiliki ciri khusus atau identitas dari pemiliknya. Darah dapat dianalisa golongannya untuk mengetahui pemilik dari darah tersebut. Namun dalam perjalanan waktu, darah akan mengalami degradasi sehingga akan terjadi perubahan golongan menjadi golongan darah O palsu. Salah satu faktor penyebabnya adalah adanya aktivitas mikroorganisme yang tumbuh pada darah. Materi dari penelitian ini adalah darah manusia yang bergologan A, B, AB dan O yang berasal dari 4 (empat) orang responden berjenis kelamin 3 (tiga) laki-laki dan 1 (satu) perempuan, berumur antara 20 – 40 tahun. Masing-masing sebanyak 150 µL diteteskan pada kain kasa steril ukuran 2 x 6 cm, kemudian dikering anginkan. Sampel disimpan pada suhu kamar (± 30 oC) selama 0, 30, 60, 90 dan 120 hari. Sampel diuji golongan darah-nya menggunakan metode absorbsi elusi berdasarkan instruksi kerja Laboratorium Forensik Polri Cabang Denpasar tahun 2016. Hasil penelitian ini menunjukkan bahwa darah kering manusia yang disimpan pada media kain kasa steril selama 30 dan 60 hari belum mengalami perubahan golongannya. Penyimpanan darah kering manusia selama 90 dan 120 hari sudah terjadi perubahan golongan darah dengan ditemukan adanya antigen A atau B yang tidak terdeteksi kembali melalui analisa absorbsi elusi pada golongan darah A, B dan AB. Penelitian ini menemukan 9 spesies jamur dan 7 spesies bakteri yang tumbuh pada sampel darah. Spesies jamur dan bakteri yang memiliki kemampuan tinggi mendegradasi semua golongan darah (A, B dan AB) adalah Acromonium carticola, Alternaria alternata, Aspergillus flavus, A. niger, Penicillium sp dan Bacillus coagulans. Sedangkan spesies jamur dan bakteri yang memiliki kemampuan tinggi mendegradasi golongan B atau AB adalah Fusarium sp, Trichoderma harzianum, T. viridae, Bacillus sp. dan Staphylococcus cohnii.

scholarly journals Bioassay of Lemongrass on Fungi Pathogen Associated with Cassava Tubers Rot in Farin Gada Market, Jos

2019 ◽  
pp. 1-8
Author(s):  
W. C. John ◽  
O. Oke ◽  
M. Ishaya ◽  
O. O. Olorundare ◽  
N. Janfa ◽  
...  
Keyword(s):  
Aspergillus Flavus ◽  
Ethanol Extract ◽  
Geotrichum Candidum ◽  
Pathogenicity Test ◽  
Potato Dextrose Agar Medium ◽  
Penicillium Sp ◽  
Significant Difference ◽  
Fusarium Sp ◽  
Plateau State ◽  
Tuber Rot

The aim of this study was to determine the effects of Lemongrass on fungal pathogen associated with cassava tuber rot. The study was carried out in the biology laboratory of the Federal College of Forestry Jos, Plateau state from March to May, 2019. Rotten and healthy cassava tubers were collected separately from Farin-Gada market Jos, fungi species were isolated from rotten cassava tubers by direct inoculation of the spoiled part on sterile Potato Dextrose Agar medium and incubated for 3-5 days, the isolated fungi were identified microscopically and macroscopically. The identified fungi were used for pathogenicity test. The antifungal effect of different concentrations of ethanol extract of lemongrass was investigated. Data collected were analyzed using one way ANOVA and the means were separated using Least Significant Difference (LSD) at (p≥0.05). The fungi isolated include, Fusarium sp, Penicillium sp, Geotrichum candidum, and Aspergillus flavus. The frequency of occurrence of the isolated fungi indicated Fusarium sp, Penicillium sp, Geotrichum candidum, and Aspergillus flavus had 30, 15, 35 and 20% respectively. 20 mL of the tested extract gave the highest inhibition of 19.07, 20.57, 18.17 mL and 18.00 mL on Fusarium spp, Penicillium spp, Geotrichum candidum and Aspergillus flavus respectively. At the 5th day of incubation the results of the pathogenicity showed that Aspergillus flavus gives the highest deterioration of 9.17 mm. The length of deterioration showed significant difference. The lemongrass extract indicated anti-fungal effect on the fungal isolates, therefore could be used to control cassava tuber rot caused by fungi.

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