Extraction, Identification and Biological Activities of Saponins in Sea Cucumber Pearsonothuria graeffei

Aims and Objectives: Secondary metabolism in marine organisms produces a diversity of biologically important natural compounds that are not present in terrestrial species. Sea cucumbers belong to the invertebrate Echinodermata and are famous for their nutraceutical, medical and food values. They are known for possession triterpenoid glycosides (saponins) with various ecological roles. The current work aimed to separate, identify and test various biological activities (antibacterial, antifungal, antileishmanial and anticancer properties) of saponins produced by the holothurian Pearsonothuria graeffei from the Red Sea, Egypt. Materials and Methods: The structures were identified by 1D and 2D NMR (1H, 13C, TOCSY, COSY, HSQC, HMBC, and ROESY) experiments and acid hydrolysis. The crude and purified fractions was analyzed using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS)/MS to identify saponins and characterize their molecular structures. Partially purified fraction, mainly containing compounds 1 and 2, was screened for its antifungal activity against three clinical isolates of Candida albicans (Candida 580 (1), Candida 581(2) and Candida MEO47228. Antileishmanial activity against Leishmania major and toxicity on colon cell-line were also evaluated. Results: Two lanostane type sulfated triterpene monoglycosides were isolated from the Holothurian Pearsonothuria graeffei from the Red Sea, Egypt. Holothurin A (1) and echinoside A (2) triterpene saponins were separated by reversed phase semi-preparative HPLC. LC50 values (µg/mL); calculated for the fraction containing saponins 1 and 2 as major constituents; against Candida albicans, Leishmania major and colon cell-line were 10, 20 and 0.50, respectively. Conclusion: Consequently, this study demonstrated the potential use of sea cucumber Pearsonothuria graeffei not only as appreciated functional food or nutraceuticals but also as the source of functional ingredients for pharmaceutical products with antifungal, antileishmanial and anticancer properties.

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Formation of hydrogen peroxide in cell culture media by apple polyphenols and its effect on antioxidant biomarkers in the colon cell line HT-29

Molecular Nutrition & Food Research ◽

10.1002/mnfr.200800456 ◽

2009 ◽

Vol 53 (10) ◽

pp. 1226-1236 ◽

Cited By ~ 38

Author(s):

Phillip Bellion ◽

Melanie Olk ◽

Frank Will ◽

Helmut Dietrich ◽

Matthias Baum ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Cell Culture ◽

Cell Line ◽

Culture Media ◽

Cell Culture Media ◽

Colon Cell ◽

Colon Cell Line ◽

Ht 29

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Determination of cell death induced by lovastatin on human colon cell line HT29 using comet assay

Toxicology Letters ◽

10.1016/j.toxlet.2011.05.419 ◽

2011 ◽

Vol 205 ◽

pp. S116-S117

Author(s):

M. Rezaei ◽

H. Kalantari ◽

M. HashemiTabar ◽

M. Jafari ◽

Z. Bahadori

Keyword(s):

Cell Death ◽

Comet Assay ◽

Cell Line ◽

Human Colon ◽

Human Colon Cell Line ◽

Colon Cell ◽

Colon Cell Line

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Novel Synthesis Method of Micronized Ti-Zeolite Na-A and Cytotoxic Activity of Its Silver Exchanged Form

Bioinorganic Chemistry and Applications ◽

10.1155/2015/428121 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 10

Author(s):

H. F. Youssef ◽

W. H. Hegazy ◽

H. H. Abo-almaged ◽

G. T. El-Bassyouni

Keyword(s):

Cytotoxic Activity ◽

Cell Line ◽

Carcinoma Cell ◽

Synthesis Method ◽

Carcinoma Cell Line ◽

Breast Adenocarcinoma ◽

Synthetic Process ◽

Colon Cell ◽

Colon Cell Line

The core-shell method is used as a novel synthetic process of micronized Ti-Zeolite Na-A which involves calcination at 700°C of coated Egyptian Kaolin with titanium tetrachloride in acidic medium as the first step. The produced Ti-coated metakaolinite is subjected to microwave irradiation at low temperature of 80°C for 2 h. The prepared micronized Ti-containing Zeolites-A (Ti-Z-A) is characterized by FTIR, XRF, XRD, SEM, and EDS elemental analysis. Ag-exchanged form of Ti-Z-Ag is also prepared and characterized. The Wt% of silver exchanged onto the Ti-Zeolite structure was determined by atomic absorption spectra. Thein vitrocytotoxic activity of Ti-Z-Ag against human hepatocellular carcinoma cell line (HePG2), colon cell line carcinoma (HCT116), lung carcinoma cell line (A549), and human Caucasian breast adenocarcinoma (MCF7) is reported. The results were promising and revealed that the exchanged Ag form of micronized Ti-Zeolite-A can be used as novel antitumor drug.

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Monitoring of the intracellular activation of 5-fluorouracil to deoxyribonucleotides in HT29 human colon cell line: application to modulation of metabolism and cytotoxicity study

Fundamental and Clinical Pharmacology ◽

10.1111/j.1472-8206.2000.tb00403.x ◽

2000 ◽

Vol 14 (2) ◽

pp. 147-154 ◽

Cited By ~ 11

Author(s):

Joseph Ciccolini ◽

Laurent Peillard ◽

Claude Aubert ◽

Patricia Formento ◽

Gérard Milano ◽

...

Keyword(s):

Cell Line ◽

Human Colon ◽

Human Colon Cell Line ◽

Colon Cell ◽

Colon Cell Line

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Arabinoxylan-Based Particles: In Vitro Antioxidant Capacity and Cytotoxicity on a Human Colon Cell Line

Medicina ◽

10.3390/medicina55070349 ◽

2019 ◽

Vol 55 (7) ◽

pp. 349 ◽

Cited By ~ 2

Author(s):

Mayra A. Mendez-Encinas ◽

Elizabeth Carvajal-Millan ◽

Agustín Rascón-Chu ◽

Humberto Astiazarán-García ◽

Dora E. Valencia-Rivera ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Cell Line ◽

Human Colon ◽

Colon Cells ◽

Antioxidant Power ◽

Human Colon Cell Line ◽

Colon Cell ◽

Colon Cell Line

Background and objectives: Arabinoxylans (AX) can gel and exhibit antioxidant capacity. Previous studies have demonstrated the potential application of AX microspheres as colon-targeted drug carriers. However, the cytotoxicity of AX gels has not been investigated so far. Therefore, the aim of the present study was to prepare AX-based particles (AXM) by coaxial electrospraying method and to investigate their antioxidant potential and cytotoxicity on human colon cells. Materials and Methods: The gelation of AX was studied by monitoring the storage (G′) and loss (G′′) moduli. The morphology of AXM was evaluated using optical and scanning electron microscopy (SEM). The in vitro antioxidant activity of AX before and after gelation was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. In addition, the effect of AX and AXM on the proliferation of human colon cells (CCD 841 CoN) was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The final G′ and G′′ values for AX gels were 293 and 0.31 Pa, respectively. AXM presented spherical shape and rough surface with a three-dimensional and porous network. The swelling ratio and mesh size of AXM were 35 g water/g AX and 27 nm, respectively. Gelation decreased the antioxidant activity of AX by 61–64 %. AX and AXM did not affect proliferation or show any toxic effect on the normal human colon cell line CCD 841 CoN. Conclusion: The results indicate that AXM could be promising biocompatible materials with antioxidant activity.

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Progastrin-derived peptides act as autocrine growth factors in the mouse colon cell line YAMC

Regulatory Peptides ◽

10.1016/0167-0115(96)87862-1 ◽

1996 ◽

Vol 64 (1-3) ◽

pp. 71

Keyword(s):

Growth Factors ◽

Cell Line ◽

Autocrine Growth ◽

Autocrine Growth Factors ◽

Mouse Colon ◽

Colon Cell ◽

Colon Cell Line

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Cytotoxic Activity of Some Spirooxindole-4H-pyran Derivatives

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2019/v31i630364 ◽

2019 ◽

pp. 1-6

Author(s):

Zeinab Faghih ◽

Zahra Faghih ◽

Masoomeh Divar ◽

Soghra Khabnadideh

Keyword(s):

Cell Line ◽

Cell Lines ◽

Anticancer Agents ◽

Biological Activities ◽

Science Research ◽

Cytotoxic Activities ◽

Medical Sciences ◽

Anticancer Properties ◽

Cancerous Cell ◽

Mcf 7

Aims: Isatin is a honored scaffold and one of the most favorable class of heterocyclic systems that possesses many interesting biological activities and well-tolerated in humans. Here a series of fifteen spirooxindole-4H-pyran derivatives containing both isatin and pyran moieties (ICa-ICo) will be examine for their anti-cancer activity. Study Design: Cytotoxic evaluation of some spirooxindole-4H-pyran derivatives in two cancerous cell lines. Place and Duration of Study: Pharmaceutical Science Research Center and Shiraz Institute for Cancer Research, Medical School in Shiraz University of Medical Sciences, Shiraz, Iran, between June 2018 and July 2019. Methodology: MTT assay was used to evaluate the cytotoxic activities of these compounds. The anticancer properties of the tested compounds were determined using A549 and MCF-7 cell lines. Results: Among the tested compounds ICc, ICd and ICf showed the best cytotoxic activities against both cancerous cell lines. Compounds ICh and ICj showed desirable cytotoxic activities against A549 cell line. Compound ICb showed desirable cytotoxic activities against MCF-7 cell line. Conclusion: We conclude that the isatin-linked pyran analog can serve as a prototype molecule for further development of a new class of anticancer agents.

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ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2016v8i4.15278 ◽

2016 ◽

Vol 8 (4) ◽

pp. 54 ◽

Cited By ~ 1

Author(s):

Burli Sanganna ◽

Havagiray R. Chitme ◽

Khanvilkar Vrunda ◽

Mohsin J. Jamadar

Keyword(s):

Antioxidant Activity ◽

Growth Inhibition ◽

Cell Line ◽

Aqueous Extract ◽

Moringa Oleifera ◽

Ethanolic Extract ◽

Colon Cell ◽

Colon Cell Line ◽

Phytochemical Constituents ◽

Ht 29

Objective: The aim of the study was to investigate the ethanolic and aqueous extract of leaves of Moringa oleifera for phytochemical constituents, antiproliferative and antioxidant activity.Methods: The ethanolic extract of leaves of Moringa oleifera, belonging to the family Moringaceae was prepared by using soxhlet apparatus and aqueous extract was prepared by using maceration process. The extract was evaluated for its phytochemical constituents. The antiproliferative effects of both extracts were checked by using MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) assay on HT-29 colon cell line and the antioxidant activity were checked by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. In antiproliferative and antioxidant activity the 5-FU (5-fluro uracil) and Ascorbic acid used as a standard drug for present results conclusion respectively.Results: The results obtained in MTT assay shown that ethanolic extract of Moringa oleifera had a more potent antiproliferative effect (growth inhibition of 62.25% at 100 μg/ml) on HT-29 colon cell line as compared to aqueous extract (% growth inhibition of 27.86 at 100 μg/ml) of Moringa oleifera. The ethanolic extract of Moringa oleifera shown more potent antioxidant activity (% inhibition of ethanolic 75.57 at 100 μg/ml) than aqueous extract (38.16 at 100 μg/ml) of Moringa oleifera. The activity shown by the extract is concentration dependent.Conclusion: In the present study we have investigated that the effect of ethanolic and aqueous leaves extracts of Moringa oleifera possess antiproliferative and antioxidant properties.

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