Production of chemotherapeutic enzyme L-asparaginase from fungal source

Background: L-Asparaginase is an antineoplastic agent used in the treatment of acute myeloid and acute lymphoblastic leukemia. The present study deals with the production of this chemotherapeutic enzyme drug from Aspergillus flavus NCIM 526. The production of enzymes was carried out using oil-extracted cakes in shake flask culture. Process parameters like carbon and nitrogen sources were taken into account. Methods: A total of six isolates were used to screen out efficient microorganisms for enzyme production. Aspergillus flavus NCIM 526 exhibited 138 IU/ml of enzyme activity in oil extracted mix cake after 96 hours of the incubation period. Molasses and l-asparagine were proved the best carbon and nitrogen sources for enzyme production. The enzyme was purified by column chromatography and the finest enzyme exhibited specific activity of 28 IU/mg. Results and Discussion: The fungal enzyme exhibited low Km values as compared with standard E. coli L-asparaginase, proving more substrate affinity of fungal enzyme than bacterial enzymes. Conclusion: The study explored the Aspergillus flavus NCIM 526 as a potential fungal source for high yield production of antileukemic enzyme drugs.

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Optimization for the Production of Cellulase Enzyme from Municipal Solid Waste Residue by Two Novel Cellulolytic Fungi

Biotechnology Research International ◽

10.4061/2011/810425 ◽

2011 ◽

Vol 2011 ◽

pp. 1-8 ◽

Cited By ~ 72

Author(s):

S. P. Gautam ◽

P. S. Bundela ◽

A. K. Pandey ◽

Jamaluddin Khan ◽

M. K. Awasthi ◽

...

Keyword(s):

Municipal Solid Waste ◽

Solid Waste ◽

Enzyme Production ◽

Nitrogen Sources ◽

Cellulase Production ◽

Production Medium ◽

Optimum Temperature ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Alternative Carbon Source

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

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Carbon and Nitrogen Sources Effect on Pectinase Synthesis by Aspergillus niger Under Submerged Fermentation

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2906 ◽

2021 ◽

Vol 18 (1) ◽

pp. 185-195

Author(s):

Gousiya Begum ◽

Srinivas Munjam

Keyword(s):

Wheat Bran ◽

Submerged Fermentation ◽

Enzyme Production ◽

Paper Industry ◽

Nitrogen Sources ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Maximum Production ◽

Fungal Strains ◽

Enhanced Production

Pectinases are the commercial enzymes that are abundantly employed in various industries like fruit juice industries for clarification, wine indutsry and paper industry for bleaching up pulp. The present work was done on culture conditions optimization for production of pectinases under submerged fermentation using wheat bran as a substrate. Fungal strains were isolated from vegetable waste dump yard soils of Warangal district of Telangana state and screened for their activity on pectin agar medium. Among 30 isolates, two fungal strains showed good activity and identified them as A. niger and A. flavus. The effects of the different carbon and nitrogen sources on pectinases viz. exo-PG, endo-PG, endo-PL and PME by A. niger with 1% wheat bran was carried out in submerged fermentation. These studies revealed that carbon and nitrogen sources have shown considerable influence on enzyme production. Among all the carbon sources tried, sucrose at 1% was shown to be efficient carbon source for all four types of pectinases under investigation. For endo-PG, endo-PL and PME maximum enzyme production were recorded on 8th day of incubation period but for exo-PG enhanced production was observed on 12th day. A. niger could not produce PME on 12th day from 2.50% to subsequent concentrations. Among nine different nitrogen sources were screened, maximum pectinase production was recorded in sodium nitrate at 0.2 % for A. niger. Endo-PG, endo-PL and PME maximum production were recorded on 8th day of incubation and for exo-PG maximum production was observed on 12th day. No PME production was observed in A. niger on 12th day.

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Improvement in raw sago starch degrading enzyme production from Acremonium sp. endophytic fungus using carbon and nitrogen sources

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(00)00243-x ◽

2000 ◽

Vol 27 (7) ◽

pp. 511-515 ◽

Cited By ~ 11

Author(s):

Yetti Marlida ◽

Nazamid Saari ◽

Zaiton Hassan ◽

Son Radu

Keyword(s):

Endophytic Fungus ◽

Enzyme Production ◽

Nitrogen Sources ◽

Sago Starch ◽

Carbon And Nitrogen Sources ◽

Degrading Enzyme ◽

Carbon And Nitrogen

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Caseinase Production and Media Optimization from Bacillus subtilis

Revista de Chimie ◽

10.37358/rc.20.11.8368 ◽

2020 ◽

Vol 71 (11) ◽

pp. 1-9

Author(s):

Noor Nihad Abdul Hussein ◽

Aseel I. Ibrahim ◽

Firas Hashim Kamar ◽

Arelia Cristina Nechifor

Keyword(s):

Bacillus Subtilis ◽

Enzyme Production ◽

Paper Industry ◽

Nitrogen Sources ◽

The Body ◽

Biochemical Tests ◽

Isolation And Identification ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Sequencing Result

Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat. Casein can be an instant energy source to the body and involves in muscle building. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. This is followed by the production of enzyme and its purification. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.

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Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

10.1101/2021.02.02.429175 ◽

2021 ◽

Author(s):

Mahdi Hamed ◽

Ahmed A Osman ◽

Mustafa Ateş

Keyword(s):

Enzyme Activity ◽

Enzyme Production ◽

Enzyme Level ◽

Nitrogen Sources ◽

Quantitative Detection ◽

Phenol Red ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Fungal Isolates ◽

The Impact

AbstractObjectiveTo semi-quantitively screen filamentous fungi isolated from different habitats for L-asparaginase production by three indicators; phenol red, cresol red and bromothymol blue and to examine the impact of different carbon and nitrogen sources on the enzyme production using different fungal isolates.Materials and methodsFifty-five fungal isolates were tested for L-asparaginase production by plate assay using Modified Czapek-Dox (MCD) medium. The enzyme activity was estimated using the Nessler method which measures the concentration of ammonia formed owing to the enzyme action on the substrate. The impact of nitrogen and carbon sources on the enzyme production was done by using the best three L-asparaginase producers from the semi-quantitative screening.Results and conclusionsA total of 53/55 (96.36%) fungal isolates were L-asparaginase producing strains, of them, Cladosporium tenuissimum, Penicillium camembertii and Aspergillus carneus showed high enzyme production. Production of L-asparaginase was higher with the glucose and urea as carbon and nitrogen sources, respectively. The highest enzyme level (5,558 U/ml) was produced by C. tenuissimum in a glucose-containing medium. This study shows that P. camemberti, A. carneus, and C. tenuissimum are good L-asparaginase producers and thus could be used for L-asparaginase production

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