Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro

SARS-CoV-2 is a newly emerging virus that currently lacks curative treatments. Lactoferrin (LF) is a naturally occurring non-toxic glycoprotein with broad-spectrum antiviral, immunomodulatory and anti-inflammatory effects. In this study, we assessed the potential of LF in the prevention of SARS-CoV-2 infection in vitro. Antiviral immune response gene expression was analyzed by qRT-PCR in uninfected Caco-2 intestinal epithelial cells treated with LF. An infection assay for SARS-CoV-2 was performed in Caco-2 cells treated or not with LF. SARS-CoV-2 titer was determined by qRT-PCR, plaque assay and immunostaining. Inflammatory and anti-inflammatory cytokine production was determined by qRT-PCR. LF significantly induced the expression of IFNA1, IFNB1, TLR3, TLR7, IRF3, IRF7 and MAVS genes. Furthermore, LF partially inhibited SARS-CoV-2 infection and replication in Caco-2 intestinal epithelial cells. Our in vitro data support LF as an immune modulator of the antiviral immune response with moderate effects against SARS-CoV-2 infection.

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The anti-apoptotic and anti-inflammatory effect of Lactobacillus acidophilus on Shigella sonnei and Vibrio cholerae interaction with intestinal epithelial cells: A comparison between invasive and non-invasive bacteria

PLoS ONE ◽

10.1371/journal.pone.0196941 ◽

2018 ◽

Vol 13 (6) ◽

pp. e0196941 ◽

Cited By ~ 6

Author(s):

Shabnam Zeighamy Alamdary ◽

Bita Bakhshi ◽

Sara Soudi

Keyword(s):

Epithelial Cells ◽

Vibrio Cholerae ◽

Lactobacillus Acidophilus ◽

Intestinal Epithelial Cells ◽

Shigella Sonnei ◽

Intestinal Epithelial ◽

Non Invasive ◽

Anti Inflammatory ◽

Inflammatory Effect

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Butyrate mediates anti-inflammatory effects of Faecalibacterium prausnitzii in intestinal epithelial cells through Dact3

10.21203/rs.3.rs-28864/v1 ◽

2020 ◽

Author(s):

Marion Lenoir ◽

Rebeca Martin ◽

Edgar Torres-Maravilla ◽

Sead Chadi ◽

Pamela González-Dávila ◽

...

Keyword(s):

Epithelial Cells ◽

Molecular Mechanisms ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial ◽

Commensal Bacterium ◽

Jnk Pathway ◽

Faecalibacterium Prausnitzii ◽

Anti Inflammatory

Abstract BackgroundThe commensal bacterium Faecalibacterium prausnitzii plays a key role in inflammatory bowel disease (IBD) pathogenesis and serves as a general health biomarker in humans. However, the host molecular mechanisms that underlie its anti-inflammatory effects remain unknown.MethodsA transcriptomic approach on human intestinal epithelial cells (HT-29) that were stimulated with TNF-α and exposed to F. prausnitzii culture supernatant (SN) was used. Modulation of the most upregulated gene after F. prausnitzii SN contact was validated both in vitro and in vivo.ResultsF. prausnitzii SN upregulates the expression of Dact3, a gene linked to the Wnt/JNK pathway. Interestingly, when we silenced Dact3 expression, the effect of F. prausnitzii SN was lost. Butyrate was identified as the F. prausnitzii effector responsible for Dact3 modulation. Dact3 upregulation was also validated in vivo in both healthy and inflamed mice treated with either F. prausnitzii SN or the live bacteria, respectively. Finally, we demonstrated by colon transcriptomics that gut microbiota directly influences Dact3 expression.ConclusionsOur results provide new clues about the host molecular mechanisms involved in the anti-inflammatory effects of the beneficial commensal bacterium F. prausnitzii.*Contributed equally to this work

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Anti-Inflammatory Properties of Streptococcus salivarius, a Commensal Bacterium of the Oral Cavity and Digestive Tract

Applied and Environmental Microbiology ◽

10.1128/aem.03133-13 ◽

2013 ◽

Vol 80 (3) ◽

pp. 928-934 ◽

Cited By ~ 50

Author(s):

Ghalia Kaci ◽

Denise Goudercourt ◽

Véronique Dennin ◽

Bruno Pot ◽

Joël Doré ◽

...

Keyword(s):

Epithelial Cells ◽

Oral Cavity ◽

Mononuclear Cells ◽

Inflammatory Responses ◽

Intestinal Epithelial Cells ◽

Intestinal Epithelial ◽

Streptococcus Salivarius ◽

Content Type ◽

Anti Inflammatory

ABSTRACTStreptococcus salivariusis one of the first colonizers of the human oral cavity and gut after birth and therefore may contribute to the establishment of immune homeostasis and regulation of host inflammatory responses. The anti-inflammatory potential ofS. salivariuswas first evaluatedin vitroon human intestinal epithelial cells and human peripheral blood mononuclear cells. We show that liveS. salivariusstrains inhibitedin vitrothe activation of the NF-κB pathway on intestinal epithelial cells. We also demonstrate that the liveS. salivariusJIM8772 strain significantly inhibited inflammation in severe and moderate colitis mouse models. Thesein vitroandin vivoanti-inflammatory properties were not found with heat-killedS. salivarius, suggesting a protective response exclusively with metabolically active bacteria.

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