Isoliquiritigenin Inhibits Proliferation and Induces Apoptosis via Alleviating Hypoxia and Reducing Glycolysis in Mouse Melanoma B16F10 Cells

2016 ◽  
Vol 11 (2) ◽  
pp. 215-227 ◽  
Author(s):  
Yanming Wang ◽  
Jun Ma ◽  
Xinyan Yan ◽  
Xiaoyu Chen ◽  
Lingling Si ◽  
...  
Keyword(s):  
Mouse Melanoma ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Jolkinolide B induces apoptosis and inhibits tumor growth in mouse melanoma B16F10 cells by altering glycolysis

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Caixia Gao ◽  
Xinyan Yan ◽  
Bo Wang ◽  
Lina Yu ◽  
Jichun Han ◽  
...  
Keyword(s):  
Tumor Growth ◽  
Mouse Melanoma ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Recombinant human PRAME immunization reducesPRAME-expressing tumor growth in mice

2018 ◽  
Vol 17 (3) ◽  
pp. 36-42
Author(s):  
Yu. P. Finashutina ◽  
N. A. Lyzhko ◽  
N. N. Kasatkina ◽  
L. A. Kesaeva ◽  
V. V. Tikhonova ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Tumor Volume ◽  
High Titer ◽  
Mouse Melanoma ◽  
B16f10 Cells ◽  
Gene Coding ◽  
Melanoma B16f10 ◽  
Tumor Types ◽  
Human Recombinant Protein ◽  
Immunization Series

Intrоduction.Human antigen PRAME is preferentially expressed in a number of different tumor types and may be a potent target for anti-tumor immunotherapy.Purpose.To study anti-tumor action of immunogenic mix recombinant PRAME protein and adjuvant in mice with innate immunity.Materials and methods.C57BL/6 female mice were used for immunization with purified human recombinant protein PRAME. Human PRAME gene coding sequence was cloned in mammalian expressing vector pCEP4 and resulting plasmid was introduced in mouse melanoma B16F10 cells by transfection followed by RQ-PCR, Western blot and flow-cytometry analysis. Then stably PRAME-transfected melanoma cells were injected in mice.Results.The mouse melanoma B16F10 cells stably expressing human PRAME protein were obtained. We demonstrate the 10-fold decreased tumor volume in mice with melanoma B16F10 expressing human PRAME after preventive immunization series with recombinant PRAME protein. The tumor volume reducing was correlated with high titer (6.14 × 10 5) of anti-PRAME antibodies in mice sera.Conclusion.These data indicate that recombinant protein PRAME is immunogenic and may be a potent antigen for immunotherapuetics studies.

scholarly journals Unveiling the Differential Antioxidant Activity of Maslinic Acid in Murine Melanoma Cells and in Rat Embryonic Healthy Cells Following Treatment with Hydrogen Peroxide

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4020
Author(s):  
Khalida Mokhtari ◽  
Amalia Pérez-Jiménez ◽  
Leticia García-Salguero ◽  
José A. Lupiáñez ◽  
Eva E. Rufino-Palomares
Keyword(s):  
Antioxidant Enzyme ◽  
Cell Lines ◽  
Enzyme Activities ◽  
Biological Properties ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Glutathione S Transferase ◽  
Maslinic Acid ◽  
B16f10 Cells ◽  
Melanoma B16f10

Maslinic acid (MA) is a natural triterpene from Olea europaea L. with multiple biological properties. The aim of the present study was to examine MA’s effect on cell viability (by the MTT assay), reactive oxygen species (ROS levels, by flow cytometry) and key antioxidant enzyme activities (by spectrophotometry) in murine skin melanoma (B16F10) cells compared to those on healthy cells (A10). MA induced cytotoxic effects in cancer cells (IC50 42 µM), whereas no effect was found in A10 cells treated with MA (up to 210 µM). In order to produce a stress situation in cells, 0.15 mM H2O2 was added. Under stressful conditions, MA protected both cell lines against oxidative damage, decreasing intracellular ROS, which were higher in B16F10 than in A10 cells. The treatment with H2O2 and without MA produced different responses in antioxidant enzyme activities depending on the cell line. In A10 cells, all the enzymes were up-regulated, but in B16F10 cells, only superoxide dismutase, glutathione S-transferase and glutathione peroxidase increased their activities. MA restored the enzyme activities to levels similar to those in the control group in both cell lines, highlighting that in A10 cells, the highest MA doses induced values lower than control. Overall, these findings demonstrate the great antioxidant capacity of MA.

Effect of downregulation of ZEB1 on vimentin expression, tumour migration and tumourigenicity of melanoma B16F10 cells and CSCs

2014 ◽  
Vol 38 (4) ◽  
pp. 452-461 ◽  
Author(s):  
Jun Dou ◽  
Xiangfeng He ◽  
Yurong Liu ◽  
Yaqian Wang ◽  
Fengshu Zhao ◽  
...  
Keyword(s):  
Vimentin Expression ◽  
B16f10 Cells ◽  
Melanoma B16f10

T-cadherin suppresses the cell proliferation of mouse melanoma B16F10 and tumor angiogenesis in the model of the chorioallantoic membrane

2010 ◽  
Vol 41 (4) ◽  
pp. 217-226 ◽  
Author(s):  
E. I. Yurlova ◽  
K. A. Rubina ◽  
V. Yu. Sysoeva ◽  
G. V. Sharonov ◽  
E. V. Semina ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Angiogenesis ◽  
Chorioallantoic Membrane ◽  
Mouse Melanoma ◽  
Melanoma B16f10

scholarly journals Anti-metastatic Effect of Garlic Hexane Extract on Lung Metastasis Induced by Melanoma B16F10 Cells in Mice

2016 ◽  
Vol 26 (2) ◽  
pp. 259-264
Author(s):  
Min Jung Ko ◽  
Seetharaman Rajasekar ◽  
Ziyu Wang ◽  
Mei Li ◽  
Jung Ho Kwak ◽  
...  
Keyword(s):  
Lung Metastasis ◽  
Hexane Extract ◽  
B16f10 Cells ◽  
Melanoma B16f10

scholarly journals Graphene-Induced Hyperthermia (GIHT) Combined With Radiotherapy Fosters Immunogenic Cell Death

2021 ◽  
Vol 11 ◽  
Author(s):  
Malgorzata J. Podolska ◽  
Xiaomei Shan ◽  
Christina Janko ◽  
Rabah Boukherroub ◽  
Udo S. Gaipl ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Combined Treatment ◽  
Infrared Light ◽  
Immunogenic Cell Death ◽  
Tumor Cell Death ◽  
Induced Hyperthermia ◽  
B16f10 Cells ◽  
Melanoma B16f10

Radiotherapy and chemotherapy are the standard interventions for cancer patients, although cancer cells often develop radio- and/or chemoresistance. Hyperthermia reduces tumor resistance and induces immune responses resulting in a better prognosis. We have previously described a method to induce tumor cell death by local hyperthermia employing pegylated reduced graphene oxide nanosheets and near infrared light (graphene-induced hyperthermia, GIHT). The spatiotemporal exposure/release of heat shock proteins (HSP), high group mobility box 1 protein (HMGB1), and adenosine triphosphate (ATP) are reported key inducers of immunogenic cell death (ICD). We hypothesize that GIHT decisively contributes to induce ICD in irradiated melanoma B16F10 cells, especially in combination with radiotherapy. Therefore, we investigated the immunogenicity of GIHT alone or in combination with radiotherapy in melanoma B16F10 cells. Tumor cell death in vitro revealed features of apoptosis that is progressing fast into secondary necrosis. Both HSP70 and HMGB1/DNA complexes were detected 18 hours post GIHT treatment, whereas the simultaneous release of ATP and HMGB1/DNA was observed only 24 hours post combined treatment. We further confirmed the adjuvant potential of these released DAMPs by immunization/challenge experiments. The inoculation of supernatants of cells exposed to sole GIHT resulted in tumor growth at the site of inoculation. The immunization with cells exposed to sole radiotherapy rather fostered the growth of secondary tumors in vivo. Contrarily, a discreet reduction of secondary tumor volumes was observed in mice immunized with a single dose of cells and supernatants treated with the combination of GIHT and irradiation. We propose the simultaneous release of several DAMPs as a potential mechanism fostering anti-tumor immunity against previously irradiated cancer cells.

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