Phenolic Content, Antibacterial and Antioxidant Activities of Moroccan propolis

Background:Propolis contains compounds with antibacterial and antioxidant capacities, such as phenolic compounds and flavonoids. The aim of this study was to investigate the antibacterial and antioxidant effects of six propolis samples from northern Morocco and to determine their content of polyphenols and flavonoids.Methods:The total polyphenols and flavonoids of Ethanol Extracts (EEP) were evaluated by colorimetric assays. To test the antibacterial activity, the well diffusion and micro-dilution methods were adopted. The kinetic of bacterial growth in the presence of EEP was tested. The antioxidant activity was evaluated using three techniques: reducing power, β-carotene bleaching test, and DPPH scavenging capacity assay.Results:The EEPs were found to have high total phenolic content (65.09 - 122.00 mg GAE/g) and flavonoids (41.95 - 50.44 mg QE/g). The screening of the antibacterial activity showed that all tested bacteria were sensitive to EEP except Escherichia coli. The inhibitory zones varied from 11.50 ± 0.70 to 32.00 ± 0.70 mm, and MIC and MBC ranged from 0.07 to 5 mg/mL and 0.07 to >5 mg/mL, respectively. The antioxidant activity was proportional to the phenolic content of propolis. The IC50 varied from 48.70 to 156.00 mg/mL and the RAA% ranged from 75.07 to 96.68 %.

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Anti-urease, total phenolic content and antioxidant activities of some Inula l. (Asteraceae) taxa in Turkey

Genetika ◽

10.2298/gensr2003825s ◽

2020 ◽

Vol 52 (3) ◽

pp. 825-834

Author(s):

Emre Sevindik ◽

Sinem Aydin ◽

Mehmet Paksoy ◽

Bahar Sokmen

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Soxhlet Extraction ◽

Total Phenolic ◽

Food Industries ◽

Total Antioxidant ◽

Ethanol Extracts

The genus Inula belongs to family Asteraceae and it is medicinally important because it possesses cytotoxic, anticancer, hepatoprotective, antibacterial and anti-inflammatory properties. The aim of this research was to explore antioxidant and anti-urease activities of fifteen Inula taxa in Turkey. Ethanol extracts of Inula taxa were obtained by Soxhlet extraction. Antioxidant activity was determined through total antioxidant activity, total phenolic content and copper reducing antioxidant capacity (CUPRAC) analyses. The highest total phenolic content and total antioxidant capacity were found in I. orientalis (158.54?0.002 ?g GAE/mL) and I. macrocephala (189.16?0.002 ?g AAE/mL). CUPRAC activity increases with the increasing concentration of the extracts and the standard. Moreover, all the tested plants exhibited better anti-urease activity than standard thiourea except for I. anatolica and I. thapsoides subsp. thapsoides. Results obtained from this study demonstrate that Inula species might be utilized as a probable natural source in the pharmaceutical and food industries but further and more specific studies are needed to determine active antioxidative and anti-urease compounds in the extracts.

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Assessment of in vitro Antioxidant and Antidiabetic Capacities of Medlar (Mespilus germanica)

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111325 ◽

2018 ◽

Vol 47 (2) ◽

pp. 384-389

Author(s):

Sebnem Selen ISBILIR ◽

Sevilay Inal KABALA ◽

Hulya YAGAR

Keyword(s):

Antioxidant Activity ◽

Leaf Extract ◽

Antioxidant Activities ◽

Inhibitory Effect ◽

Total Phenolic ◽

Flower Bud ◽

Ethanol Extracts ◽

Dpph Scavenging ◽

Β Carotene

The objective of the current study was to evaluate the antioxidant activity and enzyme inhibitory effect of different parts of medlar including fruit, leaf and flower bud by using various in vitro methods, and also determination of total phenolic and flavonoid content in the samples. Ethanol extracts of medlar parts were prepared and their antioxidant activities were determined using 1,1-diphenyl-2-picryl-hydrazil (DPPH•) scavenging and β-carotene bleaching methods. The leaf extract showed the strongest antioxidant activity. DPPHradical scavenging activity was in the order of BHA > leaf > bud > fruit. This ordering was the same for β-carotene bleaching activity, tocopherol > leaf > bud > fruit. The highest total phenolic (60.3 ± 1.69 mg GAE g-1 extract) and flavonoid (14.77 ± 1.15 mg QE g-1 extract) content were determined in leaf extract. For possible antidiabetic effects of extracts, α-amylase and α-glucosidase inhibitory activities were investigated, the bud extract showed the highest inhibition activities among the all extracts.

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Estimation of Total Phenolics and Flavonoid Content and In-vitro Antioxidant Activity of Drypetes sepiaria Stem (Wight and Arn.) Pax and K. Hoffm.

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v5i1.8865 ◽

2015 ◽

Vol 5 (1) ◽

Author(s):

Packia M. ◽

Daffodil D. ◽

Tresina S. ◽

Mohan R.

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Antioxidant Activities ◽

Reducing Power ◽

Model Systems ◽

Aluminium Chloride ◽

Total Phenolic ◽

Ethanol Extracts ◽

In Vitro Antioxidant Activity

The total phenolics, flavonoids and in vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of stem of Drypetes sepiaria were determined using various antioxidant model systems viz, DPPH, hydroxyl, superoxide, ABTS and reducing power. Total phenolic content was estimated by Folin-Ciocalteau method. Flavonoids were determined by Aluminium chloride method. The total phenolics and flavonoids contents were found to be 0.81 g 100 g-1 and 1.12g 100 g-1 respectively in the methanol extract. Among the solvent tested, methanol and ethanol extracts of D. sepiaria stem showed potent in vitro antioxidant activities. This investigation explored that D. sepiaria stem is a potential source of natural antioxidant.

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Effect of grinding and extraction conditions on the determination of antioxidant activity and phenolic acids in barley

Journal of Food Measurement & Characterization ◽

10.1007/s11694-021-00964-0 ◽

2021 ◽

Author(s):

Bing Zhou ◽

Zhao Jin ◽

Paul B. Schwarz ◽

Yin Li

Keyword(s):

Phenolic Acid ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Dpph Scavenging

AbstractIn the present study, the objective was to evaluate the effects of barley kernel size, grind level, and extract solvent on the antioxidant activities associated with total phenolic content and phenolic acid compositions. Three barley varieties (Kindred, Azure, and Tradition), were used and the results showed that with the exception of superoxide anion radical scavenging activity, thin kernel size fraction (2.0 mm) showed much higher antioxidant activities and total phenolic content than those in the 2.4 mm and 2.8 mm fractions when averaged across barley cultivar, grind level, and extract solvent. A similar trend was found for individual phenolic acid compositions among kernel sizes. Simple correlation analysis revealed that total phenolic content showed strong correlation with DPPH· scavenging activity, ABTS + scavenging activity, and reducing power. Generally, fine grind and 80% acetone extract showed averaged highest values in antioxidant activities, total phenolic content, and individual phenolic acid compositions. Stepwise linear regression showed that extract solvent was the most important factor for DPPH· scavenging activity, ABTS + scavenging activity, reducing power, and iron chelating activity. The sum of syringic acid and caffeic acid exhibited a dominant role in explaining the major variation in antioxidant activities except for superoxide anion radical scavenging activity.

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In Vitro Assessment of Selected Medicinal Plants Used by the Bapedi Community in South Africa for Treatment of Bacterial Infections

Journal of Evidence-Based Integrative Medicine ◽

10.1177/2515690x18762736 ◽

2018 ◽

Vol 23 ◽

pp. 2515690X1876273 ◽

Cited By ~ 3

Author(s):

Refilwe Given Kudumela ◽

Peter Masoko

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Medicinal Plants ◽

Bacterial Infections ◽

Antioxidant Activities ◽

Scientific Information ◽

Radical Scavenging ◽

Reducing Power ◽

Limpopo Province ◽

Total Phenolic

There is very little scientific information on indigenous plants used for medicinal purposes. Therefore, the aim of the study was to evaluate the antibacterial and antioxidant activities of the leaves of Commelina africana, Elephantorrhiza elephantina, Dombeya rotundifolia, and the whole plant excluding the roots of Schkuhria pinnata indigenous medicinal plants from the Limpopo Province, which may be used for the treatment in humans infected with bacterial pathogens. The screening of different phytoconstituents using standard methods revealed the presence of terpenoids, flavonoids, tannins, and saponins. The total phenolic, tannin, and flavonoid content were estimated using spectrophotometric methods. D rotundifolia had the highest amounts of phenol (259.00 ± 2.65 mg of gallic acid equivalent [GAE]/g), tannin (330.33 ± 15.63 mg of GAE/g), and flavonoid (19.90 ± 0.75 mg of quercetin equivalent [QE]/g) content, while S pinnata had the least. All the plants had antibacterial activity against Escherichia coli and Pseudomonas aeruginosa with the bioautography method. The microbroth dilution assay revealed antibacterial activity with minimum inhibitory concentration values raining from 0.02 to 2.50 mg/mL. D rotundifolia had the highest antioxidant activity in both the free radical scavenging and ferric reducing power methods. S pinnata and C africana extracts had high antibacterial activity, while D rotundifolia had high antioxidant activity, which made them potential plants for isolation of bioactive compounds and possible application in the pharmaceutical industry.

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Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia

Asian Journal of Agriculture ◽

10.13057/asianjagric/g03101 ◽

2019 ◽

Vol 3 (01) ◽

pp. 1-5 ◽

Cited By ~ 1

Author(s):

AMALIA INDAH PRIHANTINI ◽

KANTI DEWI RIZQIANI

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Dry Extract ◽

Antioxidant Assays ◽

Β Carotene

Abstract. Prihantini AI, Rizqiani K. 2019. Various antioxidant assays of agarwood extracts (Gyrinops versteegii) from West Lombok, West Nusa Tenggara, Indonesia. Asian J Agric 3: 1-5. However, the species have not been widely explored as a source of natural products in particular antioxidant agents, which protect cells from damage caused by free radicals. The present study was aimed to evaluate antioxidant activities of agarwood extracts from West Nusa Tenggara using various antioxidant assays. The antioxidant activity of leaf, fruit and fruit bark extracts were investigated based on DPPH radicals scavenging activity, reducing power, and β-carotene bleaching assays. The total phenolic content was also investigated. The result showed that leaf extract revealed the strongest antioxidant activity on all assays performed such as DPPH radicals scavenging activity (IC50 22.13±0.71 μg/mL); reducing power (251.85±0.03 mg QE/g dry extract); and β-carotene bleaching activity (IC50 24.23±2.60 μg/mL). The total phenolic content (TPC) in the leaf was higher (184.90±0.76 mg GAE/g dry extract) than fruit bark and bark extracts. The high content of phenolic compounds in G. versteegii leaves indicated that these compounds might contribute to the antioxidant activities. In conclusion, these findings showed that G. versteegii leaves are potential for development as an antioxidant source.

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Correlation of Total Phenolic, Flavonoid and Carotenoid Content of Sesbania sesban (L. Merr) Leaves Extract with DPPH Scavenging Activties

International Journal of Pharmacognosy and Phytochemical Research ◽

10.25258/ijpapr.v9i1.8046 ◽

2017 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Sani N. Fitriansyah ◽

Irda Fidrianny ◽

Komar Ruslan

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Carotenoid Content ◽

Total Phenolic ◽

Sesbania Sesban ◽

Total Carotenoid Content ◽

Dpph Method ◽

Dpph Scavenging

Antioxidants are found in many plants and can neutralize free radicals. Sesbania sesban (L.)Merr is a plant that has been used empirically by Indonesian people and its proven have many pharmacologycal activity. The objectives of this research were to study antioxidant activity Sesbania sesban leaves extract using DPPH (2,2-diphenyl-1-picrylhydrazyl) method and correlation with its total phenolic, flavonoid and carotenoid content. Extraction was performed by reflux using different polarity solvent. The extracts were vaporated using rotary evaporator. Antioxidant activity was tested using DPPH assay. Determination of total phenolic, flavonoid and carotenoid content were performed by UV-visible spectrophotometry and its correlation with inhibitory concentration 50 (IC50) DPPH scavenging activity were analyzed by Pearson’s method. Nhexane, ethyl acetate and ethanol extracts of Sesbania sesban leaves had IC50 DPPH  50 g/ml and it can be classified as very strong antioxidant. Ethanol extract of Sesbania sesban had the highest of total phenolic content (5.18 g GAE/100 g) and highest total flavonoid content (4.56 g QE/100 g), while the highest total carotenoid content (4.56 g BE/100 g) was given by n-hexane extract. Total phenolic content in Sesbania sesban leaves extracts had significant and negative correlation with their IC50 DPPH scavenging activities. Phenolic compounds in Sesbania sesban leaves extracts were contributor major in its antioxidant activities by DPPH method.

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Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities

Food Research ◽

10.26656/fr.2017.5(6).253 ◽

2021 ◽

Vol 5 (6) ◽

pp. 187-196

Author(s):

R. Ghosh ◽

P. Barua ◽

O. Sikder ◽

S. Saha ◽

S. Mojumder ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

In Vitro Models ◽

Frap Assay ◽

Reducing Power Assay ◽

Dpph Scavenging

Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.

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Effects of Storage Time and Temperature on Antioxidants in Juice from Momordica charantia L. and Momordica charantia L. var. abbreviata Ser.

Molecules ◽

10.3390/molecules25163614 ◽

2020 ◽

Vol 25 (16) ◽

pp. 3614

Author(s):

Yung-Sheng Lin ◽

Wen-Ying Huang ◽

Pang-Yen Ho ◽

Shiou-Yih Hu ◽

Ying-Yi Lin ◽

...

Keyword(s):

Antioxidant Activity ◽

Storage Time ◽

Storage Temperature ◽

Antioxidant Activities ◽

Reducing Power ◽

Dry Weight ◽

Momordica Charantia ◽

Total Phenolic ◽

High Storage Temperature ◽

Dpph Scavenging

This study determined the antioxidant activities of juice from Momordica charantia L. (MC) and MC var. abbreviata Ser. (MCVAS) by analyzing 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging ability, ferric reducing power (FRP), and total phenolic content (TPC). The effects of storage time and storage temperature on these antioxidant activities were investigated. Liquid chromatography–mass spectrometry was conducted to identify the major components of MC and MCVAS. The results revealed that the antioxidant activity of MCVAS was better than that of MC, possibly because of richer components of MCVAS. For MC and MCVAS, the scavenging concentrations of 50% DPPH were 3.33 and 1.19 mg/mL, respectively; moreover, the FRP values were 68.93 and 118.14 mg ascorbic acid equivalent/g dry weight, respectively; and the TPC values were 8.15 and 11.47 mg gallic acid equivalent/g dry weight, respectively. The antioxidant activities of MC and MCVAS decreased with storage time. High storage temperature decreased antioxidant activity more quickly than a low temperature. In addition, MC had exhibited a faster decline in DPPH scavenging ability and FRP than MCVAS during 24-day storage, but no difference was observed in TPC.

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SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

Jurnal Natural ◽

10.30862/jn.v7i1.678 ◽

2008 ◽

Vol 7 (1) ◽

Author(s):

Bimo Budi Santoso

Keyword(s):

Antioxidant Activity ◽

Medicinal Plants ◽

Antitumor Activity ◽

Traditional Method ◽

Extraction Efficiency ◽

Phenolic Content ◽

Antioxidant Activities ◽

Total Phenolic ◽

Phenolic Contents ◽

Total Phenolic Contents

Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed. The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents, A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R2= 0,77) and methanol (R2 = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.

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