Antiproliferative Activities on Renal, Prostate and Melanoma Cancer Cell Lines of Sarcopoterium spinosum Aerial Parts and its Major Constituent Tormentic Acid

A series of eight polymethoxychalcone Mannich base derivatives 2a-2h was synthesized via the microwave-assisted Mannich reaction of natural product 2'-hydroxy-3,4,4',5,6'-pentamethoxychalcone (1) with various secondary amines and formaldehyde. Compared to conventional heating method (80°C), the microwave-assisted method (700W, 65°C) is efficient with short reaction time (0.5-1 h) and good yields (74-88%). The antiproliferative activities of eight Mannich base derivatives were evaluated in vitro on a panel of three human cancer cell lines (Hela, HCC1954 and SK-OV-3) by CCK-8 assay. The results showed that all of the Mannich base derivatives exhibited potential antiproliferative activities on tested cancer cell lines with the IC50 values of 9.13-48.51 µM. Some active compounds exhibited more activity as compared to positive control cis-Platin. Among them, compound 2b revealed to have the strongest antiproliferative activity against all the three cancer cell lines with IC50 values ranging from 9.13 to 11.24 µM.

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Underutilized Mexican Plants: Screening of Antioxidant and Antiproliferative Properties of Mexican Cactus Fruit Juices

Plants ◽

10.3390/plants10020368 ◽

2021 ◽

Vol 10 (2) ◽

pp. 368

Author(s):

Elda M. Melchor Martínez ◽

Luisaldo Sandate-Flores ◽

José Rodríguez-Rodríguez ◽

Magdalena Rostro-Alanis ◽

Lizeth Parra-Arroyo ◽

...

Keyword(s):

Cell Proliferation ◽

Liver Cancer ◽

Cell Line ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

In Vitro Assays ◽

Total Phenolic ◽

Liver Cancer Cell ◽

Antiproliferative Activities

Cacti fruits are known to possess antioxidant and antiproliferative activities among other health benefits. The following paper evaluated the antioxidant capacity and bioactivity of five clarified juices from different cacti fruits (Stenocereus spp., Opuntia spp. and M. geomettizans) on four cancer cell lines as well as one normal cell line. Their antioxidant compositions were measured by three different protocols. Their phenolic compositions were quantified through high performance liquid chromatography and the percentages of cell proliferation of fibroblasts as well as breast, prostate, colorectal, and liver cancer cell lines were evaluated though in vitro assays. The results were further processed by principal component analysis. The clarified juice from M. geomettizans fruit showed the highest concentration of total phenolic compounds and induced cell death in liver and colorectal cancer cells lines as well as fibroblasts. The clarified juice extracted from yellow Opuntia ficus-indica fruit displayed antioxidant activity as well as a selective cytotoxic effect on a liver cancer cell line with no toxic effect on fibroblasts. In conclusion, the work supplies evidence on the antioxidant and antiproliferative activities that cacti juices possess, presenting potential as cancer cell proliferation preventing agents.

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Assessing the effect of human dental pulp mesenchymal stem cell secretome on human oral, breast, and melanoma cancer cell lines

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2021.07.029 ◽

2021 ◽

Author(s):

A. Thirumal Raj ◽

Supriya Kheur ◽

Ramesh Bhonde ◽

Vishnu R Mani ◽

Hosam Ali Baeshen ◽

...

Keyword(s):

Stem Cell ◽

Mesenchymal Stem Cell ◽

Cell Lines ◽

Cancer Cell ◽

Dental Pulp ◽

Cancer Cell Lines ◽

Melanoma Cancer ◽

Human Dental Pulp

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Triterpenoidal and Phenolic Compounds Isolated from the Aerial Parts of Helicteres hirsuta and their Cytotoxicity on Several Cancer Cell Lines

Natural Product Communications ◽

10.1177/1934578x1901400103 ◽

2019 ◽

Vol 14 (1) ◽

pp. 1934578X1901400

Author(s):

Triet Thanh Nguyen ◽

Nadine Kretschmer ◽

Eva-Maria Pferschy-Wenzig ◽

Olaf Kunert ◽

Rudolf Bauer

Keyword(s):

Phenolic Compounds ◽

Cancer Cells ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Moderate Activity ◽

Human Cancer Cell Lines ◽

Aerial Parts ◽

Helicteres Isora

Helicteres L. is one of the genera of the Sterculiaceae family with several remarkable activities. Previous studies revealed that terpenoids, flavonoids, and lignans are the dominant constituents of Helicteres species. However, information about this genus is scarce and unsystematic. Most of the phytochemical and pharmacological investigations have been mainly reported on Helicteres angustifolia and Helicteres isora, which are commonly used in China and Indonesia, respectively. In the present study, two terpenoids: 3β- O-acetylbetulinic acid (1) and simiarenol (2) together with three phenolic compounds: 4,4'-sulfinylbis(2-( tert-butyl)-5-methylphenol) (3), 7- O-methylisoscutellarein (4), 7,4'-di- O-methylisoscutellarein (5), and a mixture of stigmasterol and β-sitosterol were isolated and structurally elucidated from the aerial parts of Helicteres hirsuta Lour. Compounds 1-5 were tested for cytotoxicity on four human cancer cell lines: leukemia CCRF-CEM, breast MDA-MB-231, colon HCT116 and glioblastoma U251 cancer cells. Among them, compounds 1 and 3 showed moderate activity on CCRF-CEM and HCT116 cancer cells with IC50 values ranging from 14.6 to 31.5 μM (P < 0.05). This is the first time these compounds have been reported from this plant. To the best of our knowledge, compound 3 is novel in nature although it has been chemically synthesized before, and compounds 1, 2, and 4 are new to this plant family (Sterculiaceae).

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Correction: Colchicine metallocenyl bioconjugates showing high antiproliferative activities against cancer cell lines

Dalton Transactions ◽

10.1039/c8dt90016g ◽

2018 ◽

Vol 47 (8) ◽

pp. 2822-2822 ◽

Cited By ~ 2

Author(s):

Karolina Kowalczyk ◽

Andrzej Błauż ◽

Wojciech M. Ciszewski ◽

Anna Wieczorek ◽

Błażej Rychlik ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Dalton Trans ◽

Antiproliferative Activities

Correction for ‘Colchicine metallocenyl bioconjugates showing high antiproliferative activities against cancer cell lines’ by Karolina Kowalczyk et al., Dalton Trans., 2017, 46, 17041–17052.

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Synthesis and Biological Activity of 2-Arylidene-N-(quinolin-6-yl)hydrazine-1-carboxamides

Journal of Chemistry ◽

10.1155/2020/2189743 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Shengxian Zhao ◽

Yin Cao ◽

Zhenzhen Cui ◽

Jiayun Zhang ◽

Zhixiang Pan ◽

...

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Colony Formation Assay ◽

M Phase ◽

Antiproliferative Activities ◽

Mcf 7

A series of 2-arylidene-N-(quinolin-6-yl)hydrazine-1-carboxamides 5a–5o were synthesized and characterized. The synthesized compounds (5a–5o) were screened in vitro against three breast cancer cell lines: SKBR3, MDA-MB-231, and MCF-7 cancer cell lines by the MTT assay. According to MTT results, compounds 5k and 5l showed better antiproliferative activities over MCF-7 cell lines with IC50 values of 8.50 and 12.51 μM. Colony formation assay indicated 5k/5l treatment obviously inhibited the growth of MCF-7 cells and 5k/5l-induced cell cycle was arrested in the G2-M phase. Moreover, 5k/5l significantly increased the level of cleaved PARP and induced the apoptosis in MCF-7 cells. In addition, compared to Hela cells, MCF-7 cells were more sensitive to 5k/5l treatment.

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