scholarly journals In Vitro Assessment of Apical Leakage of Bioceramic Endodontic Sealer with Two Obturation Techniques

2018 ◽  
Vol 12 (1) ◽  
pp. 1162-1168 ◽  
Author(s):  
Arwa S. Salem ◽  
Abdul R. Mohammed Saleh ◽  
Hussein A.A. Elmasmari
Keyword(s):  
Continuous Wave ◽  
Blue Dye ◽  
Sealing Ability ◽  
Significant Difference ◽  
Single Cone ◽  
Ah Plus ◽  
Dye Extraction ◽  
Apical Leakage ◽  
One Way Anova

Purpose: The aim of this study was to evaluate the apical sealing ability of Total Fill BC sealer and AH Plus sealer with single cone and continuous wave condensation obturation techniques using dye extraction leakage method. Materials and Methods: Eighty-four extracted human single-rooted teeth with round canals were decoronated at 12 mm length and instrumented using crown down technique with the iRace rotary file system to apical file size 40 with 0.04 taper. The roots were randomly divided into four experimental groups (n=20). Group (A1) contained roots which were obturated with Total Fill BC sealer with a single cone. Group (A2) contained roots which were obturated using Total Fill BC sealer with continuous wave condensation. Group (B1) contained roots which were obturated using epoxy resin sealer (AH Plus) with a single cone. Group (B2) contained roots which were obturated using AH Plus sealer with continuous wave condensation. Apical microleakage was assessed with dye extraction method where the absorbance of leaked 1% methylene blue dye was measured using a spectrophotometer. The absorbance values were converted into concentrations, and the data was analyzed using One way ANOVA test. Results: The mean dye concentration values were 0.012, 0.016, 0.011 and 0.015 for Group A1, Group A2, Group B1 and Group B2, respectively. One-way ANOVA indicated no significant difference in dye leakage between the investigated groups (F = 0.938). Conclusion: With the limitation of the study, it was concluded that Total Fill BC was equivalent to AH Plus in apical sealing ability when using single cone or continuous wave condensation. The single cone can provide a similar apical seal of canal filling as can be achieved by using continuous wave condensation methods, in the round canal.

scholarly journals "In vitro" evaluation of the apical sealing of root canals obturated with different techniques

2003 ◽  
Vol 11 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Viviane Haiub Brosco ◽  
Norberti Bernardineli ◽  
Ivaldo Gomes de Moraes
Keyword(s):  
Continuous Wave ◽  
The Other ◽  
Blue Dye ◽  
Methylene Blue Dye ◽  
Root Canals ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Better Than ◽  
Positive Controls

The purpose of this study was to compare the apical sealing of root canals obturated with different techniques. One hundred-six human mandibular incisors were submitted to instrumentation by means of the step-back technique. After instrumentation, one hundred teeth received an impermeable coating on the external surfaces of the crown and root (except for the area nearby the apical foramen). Afterwards, they were divided in five groups containing twenty elements each, according to the obturation technique employed: 1. lateral condensation with Kerr file; 2. continuous wave of condensation technique with System B; 3. thermoplasticized injectable gutta-percha technique with the Ultrafil system; 4. mechanically thermoplasticized gutta-percha with the JS Quick-Fill system and 5. thermoplasticized gutta-percha associated to a master cone with the Microseal system. The six remaining teeth were employed as negative and positive controls. After obturation, the access cavities were sealed and the teeth were immersed in aqueous 2% methylene blue dye for 72 hours at 37ºC. After that, the teeth were longitudinally sectioned and the apical microleakage was evaluated in a stereomicroscope. The Microseal system presented the best apical sealing ability, followed by System B, JS Quick-Fill, Ultrafil and the lateral condensation technique. The statistical analysis of the results demonstrated that: 1. the Microseal system presented an apical sealing similar to System B and better than the other groups; 2. System B presented better apical sealing than the lateral condensation technique, being similar to the other groups; and 3. the lateral condensation, Ultrafil and JS Quick-Fill groups demonstrated similar sealing ability.

scholarly journals Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study

2012 ◽  
Vol 13 (6) ◽  
pp. 830-833
Author(s):  
Romel Joseph
Keyword(s):  
In Vitro Study ◽  
Blue Dye ◽  
Control Groups ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Ah Plus ◽  
And Control

ABSTRACT Aim The aim of this in vitro study was to evaluate the apical seal obtained with four root canal sealers AH 26, Sealapex, Endoflas FS and AH Plus, with lateral condensation. Materials and methods Sixty root canals were prepared using the step-back technique. The specimens were divided into four experimental groups of 12 teeth and two control groups of 12 teeth. The experimental groups were obturated by laterally condensed gutta-percha with one of the tested sealers and control groups were obturated without any sealer. Methylene blue dye penetration with centrifuging method was used to evaluate the apical sealing ability. The quantitative apical leakage of each specimen was measured after 2 weeks. Results The results showed no significant differences between all groups except between AH Plus and Endoflas FS (<0.05). AH Plus showed significantly less leakage than Endoflas FS. Conclusion AH Plus showed the least leakage compared to AH 26, Sealapex and Endoflas FS. How to cite this article Joseph R, Singh S. Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study. J Contemp Dent Pract 2012;13(6):830-833.

scholarly journals An In-vitro Study to Determine the Sealing Ability of Biodentine when used as a Class II Restorative Material

2020 ◽  
Author(s):  
Deebah Choudhary
Keyword(s):  
Base Material ◽  
In Vitro Study ◽  
Class Ii ◽  
Vitro Study ◽  
Blue Dye ◽  
P Value ◽  
Filling Material ◽  
Sealing Ability ◽  
Significant Difference

Introduction: Microleakage is a major setback to restorative dentistry. Leakage at the tooth/restoration interface may lead to numerous problems like postoperative sensitivity, recurrent caries, marginal sealing and later total failure of restoration. Aim: To determine the sealing ability (microleakage) of a new hydraulic calcium silicate cement (Biodentine) as a base material and a bulk filling material. Materials and Methods: An in-vitro study was conducted with ten freshly extracted maxillary molars from March 2019 to June 2019. Standardised Class II cavities were prepared on mesial and distal proximal surfaces. The twenty prepared samples were randomly divided into two groups (n=10)- G1 (BiodentineTM group) and G2 (BiodentineTM + Tetric N-Ceram group). The samples were subjected to thermocycling, followed by 1% methylene blue dye immersion. They were subsequently sectioned both buccolingually and mesiodistally in order to separate to proximal restorations (mesial and distal) and appreciate the amount of dye penetration at tooth/restoration interface respectively. Microleakage was evaluated by observing the samples under a stereomicroscope (30x). The data obtained was analysed using one-way ANOVA test and compared using Post-hoc test. p-value <0.05 to be considered significant. Results: Biodentine showed no significant difference between the sealing ability as a bulk filling and a base material. Group G2 (0.35) indicated comparatively less microleakage as compared to Group G1 (0.6) but the difference in microleakages in both groups was insignificant (p-value 0.083467). Conclusion: BiodentineTM exhibits microleakage when used as a bulk filling material and as a dentin substitute in Class II cavities since there is no significant difference in both the groups.

scholarly journals THE EFFECT OF THERMOCYCLING ON MARGINAL SEALING ABILITY OF MTA PLUS AND BIODENTINE WHEN USED AS ROOT-END FILLING MATERIALS

2020 ◽  
Vol 8 (11) ◽  
pp. 894-900
Author(s):  
Deebah Choudhary ◽  
Keyword(s):  
Statistical Analysis ◽  
In Vitro Study ◽  
Blue Dye ◽  
Depth Of Penetration ◽  
Jammu And Kashmir ◽  
Filling Materials ◽  
Sealing Ability ◽  
Group I ◽  
Significant Difference

Background:Marginal microleakage occurs when restorations are poorly adapted to the cavity walls which leads to penetration of bacteria, fluid and nutrient into the marginal gap. Aim: The study aimed to evaluate the effect of thermocycling on marginal sealing ability of two root-end filling materials i.e. MTA Plus and Biodentine. Materials and Methods: An in vitro study was conducted at Department of Conservative Dentistry and Endodontics in Institute of Dental Sciences (IDS), Jammu, Jammu and Kashmir, India. Forty extracted teeth with single root were decoronated maintaining a standardized length of 16mm. Biomechanical preparation and obturation was done for all the teeth. Samples were stored at 37oC and 100% humidity for seven days. Apical 3mm was resected and cavities were prepared which were randomly divided into two experimental groups with twenty samples each. The cavities were filled with MTA Plus in group I and Biodentine in group II. These groups were further divided into two subgroups of ten each (n=10). The second subgroup of both the groups did not receive any treatment and the first subgroup samples were subjected to 500 thermocycles in water bath at temperature ranges between 5oC and 55oC with a dwell time of 30 second. The samples were immersed in methylene blue dye and after through washing they were sectioned longitudinally. Examination was done under stereomicroscope to measure the depth of penetration. Statistical Analysis: The data collected were put to statistical analysis by one-way ANOVA (analysis of variance) and post hoc tests. Results: The average depth of dye penetration was least for Biodentine when thermocycling treatment was not given (0.13mm) and maximum value was depicted by MTA Plus when undergone thermal cycles (0.57mm). It is also noted that no significant difference is seen in Biodentine with or without artificial aging procedure. Conclusion:Biodentine showed least amount of microleakage, thus a better sealing ability.Alsothere was little effect of thermocycling onmicroleakage of both the materials.

scholarly journals Evaluation of the Apical Sealing Ability of Resin and Silicon Based Sealers by Dye Extraction Method: An In Vitro Study

2021 ◽  
Author(s):  
Abbas Saifuddin Unwala ◽  
Vanitha Umesh Shenoy ◽  
Sumanthini V Margasahayam ◽  
Shouvik S Mandal
Keyword(s):  
In Vitro Study ◽  
Positive Control ◽  
Control Group ◽  
P Value ◽  
Dye Penetration ◽  
Significant Difference ◽  
Ah Plus ◽  
The Mean ◽  
Dye Extraction

Introduction: Thorough, chemo-mechanical preparation of the root canal is required to completely eliminate microorganisms from the dentinal tubules. Faulty obturation in the apical area is the pathway from which bacteria and their toxins pass on to the periapical area and this leakage can be studied by sealability studies. Aim: To evaluate the apical sealing ability of three sealers i.e., AH Plus, Roekoseal and Adseal using passive dye penetration test and a dye extraction test with Methylene blue dye to evaluate the apical leakage. Materials and Methods: The present study was an experimental in-vitro study, carried out at MGM Dental College and Hospital, Navi Mumbai, Maharashtra, India over a period of 10 months (from August 2013 to May 2014). Eighty freshly extracted human permanent maxillary central incisor teeth were obtained. The crowns of the teeth were sectioned at/below cemento-enamel junction and divided into five groups: Groups I, II and III were obturated with gutta-percha and three different sealers, and groups IV and V were the two control groups. Passive dye penetration test was performed to evaluate penetration of the dye by means of capillarity followed by dye extraction test. At the end of dye extraction procedure, the supernatant solution obtained was analysed using an Ultraviolet spectrophotometer. Significance was set at 5%. Statistical analysis was performed by using IBM Statistical Package for the Social Sciences (SPSS) statistical software version 20 (Armonk, New York, United States). The normality of data in each group was tested using Shapiro-Wilk test. The mean absorbance values of the five groups were compared using one-way ANOVA. Further to compare the level of absorbance of dye among various groups, Tukey’s post-hoc test was applied. Results: The mean values of dye absorbance of all groups were as follows, AH Plus (0.02638), Roekoseal (0.06713), Adseal (0.03575), positive control group (1.36250) and negative control group (0.01894). One-way ANOVA showed p<0.001, thus indicating there was a significant difference between the dye absorbance values of all the groups. Tukey’s post-hoc test showed statistically significant difference in dye absorbance of the experimental groups when compared to positive control group. There was no significant difference in dye absorbance when experimental groups were compared to each other. When AH Plus was compared with Roekoseal, the p-value was 0.568 and 0.997 with Adseal whereas when Roekoseal was compared with Adseal the p-value was 0.778. Conclusion: The mean dye absorbance value of AH Plus was found to be least and that of Roekoseal was highest in the experimental groups. AH Plus gave the best results, as least dye was extracted from this group, indicating least dye penetration and minimum microleakage followed by Adseal and Roekoseal.

Sealing Ability of ProRoot MTA and MTA-Angelus Simulating a One-Step Apical Barrier Technique- An in vitro Study

2009 ◽  
Vol 33 (4) ◽  
pp. 305-310 ◽  
Author(s):  
Nikita Lolayekar ◽  
Sham Bhat ◽  
Sundeep Hegde
Keyword(s):  
Calcium Hydroxide ◽  
In Vitro Study ◽  
Control Group ◽  
Blue Dye ◽  
Root Segments ◽  
Sealing Ability ◽  
Significant Difference ◽  
Group A ◽  
Group B

Endodontic treatment of the pulpless tooth with an immature root apex poses a special challenge for the clinician due to lack of an apical stop against which to compact an interim dressing of calcium hydroxide, or the final obturation material. A one-visit apexification protocol with MTA (Mineral Trioxide Aggregate) has be seen as an alternative to the traditional treatment practices with calcium hydroxide [Ca(OH)2]. The aim of this study was to investigate the use of MTA as a 5mm apical barrier by comparing sealing ability of ProRoot MTA to that of MTA-Angelus. 50 freshly extracted Maxillary and Mandibular single canal sound human incisor teeth were used in this study, which were decoronated to standardized 10mm root lengths. The root segments were prepared to simulate the clinical situation of an open apex with Gates Glidden burs # 5-1 and randomly assigned into 2 experimental groups of 20 samples each (Group A: ProRoot MTA, Group B: MTA-Angelus) and 10 root segments were used as control (Group C) where no apical barrier was used. After obturation the coronal portion of all samples were sealed with Glass Ionomer and stored at 37°C and 100% humidity for 4 weeks. The root segments were then double coated with nail varnish except for the apical 1mm and apical ends of all root segments were suspended vertically in methylene blue dye for 48 hours at room temperature. After removal from the dye, the samples were sectioned longitudinally and linear dye penetration was measured using a Grid and stereomicroscope. The measurements were tabulated and statistically analyzed. The mean percentage of dye leakage for Group A was 55.5% and for Group B was 53.25%. The results showed no statistically significant difference in the sealing ability of ProRoot MTA and MTA-Angelus when used as apical barriers.

scholarly journals Assessment of Marginal Adaptation and Sealing Ability of Root Canal Sealers: An in vitro Study

2017 ◽  
Vol 18 (12) ◽  
pp. 1130-1134 ◽  
Author(s):  
Vimal Remy ◽  
Vineesh Krishnan ◽  
Tisson V Job ◽  
Madhavankutty S Ravisankar ◽  
CV Renjith Raj ◽  
...  
Keyword(s):  
Root Canal ◽  
Mineral Trioxide Aggregate ◽  
In Vitro Study ◽  
Marginal Adaptation ◽  
Root Canals ◽  
Sealing Ability ◽  
Significant Difference ◽  
Ah Plus ◽  
Root Canal Sealers

ABSTRACT Aim This study aims to compare the marginal adaptation and sealing ability [mineral trioxide aggregate (MTA)-Fillapex, AH Plus, Endofill sealers] of root canal sealers. Materials and methods In the present study, the inclusion criteria include 45 single-rooted extracted mandibular premolar teeth, with single canal and complete root formation. The sectioning of the samples was done at the cementoenamel junction using a low-speed diamond disc. Step-back technique was used to prepare root canals manually. The MTA-Fillapex, AH Plus, and Endofill sealers were the three experimental sealer groups to which 45 teeth were distributed. Under scanning electron microscope (SEM), marginal gap at sealer and root dentin interface were examined at coronal and apical halves of root canal. Results Among the three maximum marginal adaptations were seen with AH Plus sealer (4.10 ± 0.10) which is followed by Endofill sealer (1.44 ± 0.18) and MTA-Fillapex sealer (0.80 ± 0.22). Between the coronal and apical marginal adaptation, significant statistical difference (p = 0.001) was seen in AH Plus sealer. When a Mann–Whitney U-test was done on MTA-Fillapex sealer vs AH Plus sealer and AH Plus sealer vs Endofill sealer, there was a statistically significant difference (p < 0.05) found between the above two groups at coronal and apical third. Conclusion The present study proves that AH Plus sealer has a better marginal adaptation when compared with other sealers used. Clinical significance For sealing space of crown wall and main cone in root canal treatment, sealers play an important role. The other advantages of sealers are that they are used to fill voids and irregularities in root channel, secondary, lateral channels, and space between applied gutta-percha cones and also act as tripper during filling. How to cite this article Remy V, Krishnan V, Job TV, Ravisankar MS, Raj CVR, John S. Assessment of Marginal Adaptation and Sealing Ability of Root Canal Sealers: An in vitro Study. J Contemp Dent Pract 2017;18(12):1130-1134.

scholarly journals Sealing Ability of Novel Hydrophilic Single-Cone Obturation Systems in an Oval Canal: an In-Vitro Glucose Leakage Study

2014 ◽  
Vol 02 (03) ◽  
pp. 125-129
Author(s):  
Vibha Hegde ◽  
Shashank Arora
Keyword(s):  
Root Canal ◽  
Leakage Model ◽  
Group 4 ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Single Cone ◽  
Ah Plus ◽  
Group 3 ◽  
Group 2

Abstract Objective: To compare the sealing ability of three single-cone obturation systems using a glucose leakage model. Materials and Methods: A total of 90 freshly extracted human mandibular incisors with oval canals were selected and their crowns were cut. The root canal of each sample was instrumented using a rotary crown down technique and then divided into four experimental (n = 20 each) and two control groups (n = 5 each). Samples in the experimental groups were filled as follows: Group 1, cold lateral condensation using Gutta-percha/AH Plus; Group 2, single-cone C-Points/Smart-Paste Bio Sealer; Group 3, single cone Bio-Ceramic Impregnated Gutta-Percha/Endo-Sequence Bio-Ceramic Sealer; Group 4, single-cone Resilon/RealSeal SE. After 7 days, the sealing ability of root canal fillings was tested at different time intervals using glucose leakage model. Glucose leakage values were measured using a spectrophotometer and statistically analyzed. Results: The four experimental groups presented significantly different glucose leakage values at all test periods (P < 0.05). At the end of the observation period, the cumulative glucose leakage values of Groups 2 and 3 were significantly lower than those of Groups 1 and 4 (P < 0.05). Conclusion: C-Points/Smart-Paste Bio and Bio-Ceramic Impregnated Gutta-Percha/Endo-Sequence Bio-Ceramic Sealer combinations provided the superior sealing ability over the lateral condensation technique.

scholarly journals Effect of Immediate and Delayed Postpreparation on the Integrity of the Apical Seal: An in vitro Study

2012 ◽  
Vol 13 (2) ◽  
pp. 153-156
Author(s):  
Abhay Kamra ◽  
Shantanu Choudhari ◽  
Sarvesha Bhondwe ◽  
S Srilatha ◽  
Neha Chhasatia Desai ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Gutta Percha ◽  
Significant Difference ◽  
Relationship Of ◽  
The Relationship ◽  
Apical Leakage ◽  
One Way Anova

ABSTRACT Aim The aim of this in vitro study was to evaluate if there is any significant difference in apical leakage when gutta-percha is removed immediately after obturation for postspace preparation or after a week. Materials and methods Two commonly used sealers AH26 and tubliseal were used in four groups each consisting of 20 teeth each. The tooth was sectioned at the amelocemental junction to leave a root portion of 12 to 14 mm. Canals were checked for patency and prepared to No-55 K file size. Two people, using a stereomicroscope, independently evaluated each tooth-half for the extent of apical leakage. Results The leakage results were analyzed using a one-way ANOVA according to present study, immediate postpreparation is preferable than delayed postpreparation. The relationship of in vitro leakage measurements to the in vivo situation has not been established. Clinical implication Hence, immediate postpreparation is preferable than delayed postpreparation. How to cite this article Bhondwe S, Kamra A, Choudhari S, Srilatha S, Desai NC, Pandit VS. Effect of Immediate and Delayed Postpreparation on the Integrity of the Apical Seal: An in vitro Study. J Contemp Dent Pract 2012;13(2):153-156.

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