Population Dynamics of Aerobic and Anaerobic Ammonia Oxidizers in an Autotrophic Nitrogen Removal Membrane Biofilm Reactor

2008 ◽  
Vol 2008 (13) ◽  
pp. 3209-3220
Author(s):  
Kevin R. Gilmore ◽  
Nancy G. Love ◽  
Barth F. Smets ◽  
Akihiko Terada ◽  
Jay L. Garland
2006 ◽  
Vol 53 (12) ◽  
pp. 149-159 ◽  
Author(s):  
L. Gut ◽  
E. Płaza ◽  
J. Trela ◽  
B. Hultman ◽  
J. Bosander

One-year (2004) comprehensive investigations in a semi-industrial pilot plant (5 m3) were carried out with the aim of assessing the influence of operational parameters on the partial nitritation/Anammox system performance. In the system designed as a moving-bed biofilm reactor, the influent nitrogen load to the Anammox reactor was progressively increased and a stable Anammox bacterial culture was obtained. Interaction between subsequent aerobic and anaerobic conditions in the partial nitritation and Anammox reactors, respectively, granted conditions to remove nitrogen through the nitrite route. It implies that the oxygen supply can be limited to a high extent. A control strategy for the partial nitritation step relied on concomitant adjustment of the air supply with a variable influent nitrogen load, which can be monitored by both pH and conductivity measurements. In the Anammox reactor, an influent nitrite-to-ammonium ratio plays a vital role in obtaining efficient nitrogen removal. During the 1-year experimental period, the Anammox reactor was operated steadily and average nitrogen removal efficiency was 84% with 97% as the maximum value.


2016 ◽  
Vol 74 (11) ◽  
pp. 2666-2674 ◽  
Author(s):  
A. Sarti ◽  
A. W. Lamon ◽  
A. Ono ◽  
E. Foresti

This study proposes a new approach to selecting a biofilm carrier for immobilization using dissolved oxygen (DO) microsensors to measure the thickness of aerobic and anaerobic layers in biofilm. The biofilm carriers tested were polyurethane foam, mineral coal (MC), basaltic gravel, and low-density polyethylene. Development of layers in the biofilm carrier surface was evaluated using a flow cell device, and DO profiles were conducted to determine the size of the layers (aerobic and anaerobic). MC was the biofilm carrier selected due to allowing the development of larger aerobic and anaerobic layers in the biofilm (896 and 1,058 μm, respectively). This ability is supposed to improve simultaneous nitrogen removal by nitrification and denitrification biological processes. Thus, as a biofilm carrier, MC was used in a fixed-bed sequencing batch biofilm reactor (FB-SBBR) for treatment of wastewater with a high ammonia concentration (100–400 mgNH4+-N L−1). The FB-SBBR (15.0 L) was filled with matrices of the carrier and operated under alternating aeration and non-aeration periods of 6 h each. At a mean nitrogen loading rate of 0.55 ± 0.10 kgNH4+-N m−3 d−1, the reactor attained a mean nitrification efficiency of 95 ± 9% with nitrite as the main product (aerobic period). Mean denitrification efficiency during the anoxic period was 72 ± 13%.


2015 ◽  
Vol 36 (14) ◽  
pp. 1819-1827 ◽  
Author(s):  
Kai Li ◽  
Fang Fang ◽  
Jinsong Guo ◽  
Youpeng Chen ◽  
Jixiang Yang ◽  
...  

2020 ◽  
Vol 81 (5) ◽  
pp. 1071-1079
Author(s):  
Caimeng Wang ◽  
Lirong Lei ◽  
Fangrui Cai ◽  
Youming Li

Abstract In this study, the completely autotrophic nitrogen removal over nitrite (CANON) process was initiated in a sequencing batch biofilm reactor (SBBR). Then the reactor was operated under different IC/N ratios. The total inorganic nitrogen removal efficiency (TINRE) at IC/N ratios of 0.75, 1.0, 1.25, 1.5 and 2.0 were 37.0 ± 11.0%, 58.9 ± 10.2%, 73.9 ± 3.2%, 73.6 ± 1.8% and 72.6 ± 2.0%, respectively. The suitable range of IC/N ratio in this research is 1.25–2.0. The poor nitrogen removal performance at IC/N ratio of 0.75 was due to the lack of growth substrate for AnAOB and low pH simultaneously; at IC/N ratio of 1.0 this was because the substrate concentration was insufficient for fully recovering the AnAOB activities. Microbial analysis indicated that Nitrosomonas, Nitrospira and Candidatus Brocadia were the main ammonium oxidation bacteria (AOB), nitrite oxidation bacteria (NOB) and anammox bacteria (AnAOB), respectively. In addition, at IC ratios of 1.25 or higher, denitrification was promoted with the rise of IC/N ratio, which might be because the change of IC concentrations caused cell lysis of microorganisms and provided organic matter for denitrification.


2011 ◽  
Vol 378-379 ◽  
pp. 428-432
Author(s):  
Yu Qin ◽  
Jing Song Guo ◽  
Fang Fang

PCR-DGGE was applied to analyze the relationship between pH and the microbial community structure of Sequence Batch Biofilm Reactor (SBBR) autotrophic nitrogen removal process. The reactor was possessed of a high nitrogen removal efficiency at pH=8.0 where the similarity of microbial community structure between active sludge and biofilm samples was the lowest about 84.6% and the richness of bacterial community was the most abundant in biofilm compared with other pH conditions. pH=7.0 was good for the microbes in active sludge but unfavorable for anaerobic bacteria. At pH=9.0, the effects were presented with both bacterial activities and microbial community structure and when pH=6.0 the amount of microbial types dramatically dropped


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