scholarly journals Study of Salivary Stress Factor Activity in Patients with Diabetes Mellitus During the Period of Adaptation to Complete Removable Laminar Dentures

2020 ◽  
Vol 27 (3) ◽  
pp. E202038
Author(s):  
Lyubov Leybyuk ◽  
Mykola Rozhko ◽  
Olena Rozhko
Keyword(s):  
Diabetes Mellitus ◽  
Salivary Cortisol ◽  
Acrylic Resin ◽  
Stress Factor ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase ◽  
Group I ◽  
Patients With Diabetes ◽  
Cortisol Levels ◽  
Edentulous Patients

Diabetes mellitus is referred to as non-communicable epidemic of the 21st century and the fight against diabetes is one of medical and social challenges. One of its complications is damage to periodontal tissues that is accompanied by tooth loss. Any removable denture is a foreign body being a stress factor for the oral cavity. The objective of the research was to study dynamic changes in the activity of stress factor indicators, namely salivary cortisol, glucose and alpha-amylase levels in diabetic patients after prosthodontic treatment with complete removable laminar dentures. Materials and Methods. For this study, patients at the age of 45 to 74 years were divided into two groups: Group I included 52 edentulous patients with type 2 diabetes mellitus who were treated with acrylic resin complete removable laminar dentures; Group II comprised 52 edentulous patients without endocrine pathology who were treated with acrylic resin complete removable laminar dentures as well. Saliva served as the study material. The enzyme-linked immunosorbent assay was used to determine salivary cortisol levels. Glucose concentration in saliva was determined by a unified enzymatic method. Alpha-amylase activity was determined by the enzymatic colorimetric method. Results. According to the research results, before prosthodontic treatment, salivary cortisol levels of patients with diabetes mellitus were 2.4 times higher as compared to patients without somatic pathology. In phase I of adaptation to complete removable laminar dentures, in patients with diabetes mellitus, salivary cortisol concentration increased drastically by 1.4 times as compared to the indicator before prosthodontic treatment (5.61±0.83 ng/ml vs. 4.02±0.65 ng/ml, p<0.01), while in the group of patients without endocrine pathology, cortisol levels increased by 1.2 times; salivary glucose level of patients with diabetes mellitus increased by 1.3 times, while in patients without endocrine pathology, it increased by 1.1 times; in patients of Group I, salivary alpha-amylase levels increased by 1.2 times, while in patients of Group II, salivary alpha-amylase concentration increased by 1.05 times. Conclusions. In patients with diabetes mellitus, changes in saliva composition indicate a significant reduction in the adaptation mechanisms, that complicates and significantly prolongs the period of adaptation to complete removable laminar dentures.

scholarly journals Salivary Alpha Amylase Enzyme and Salivary Cortisol Level in Depression after Treatment with Fluoxetine

2021 ◽  
Vol 9 (T3) ◽  
pp. 144-149
Author(s):  
Andi Jayalangkara Tanra ◽  
Hawaidah Madeali ◽  
Mayamariska Sanusi ◽  
Saidah Syamsuddin ◽  
Sonny Teddy Lisal
Keyword(s):  
Salivary Cortisol ◽  
Depressive Disorders ◽  
Rating Scale ◽  
Antidepressant Drug ◽  
Alpha Amylase ◽  
Salivary Alpha Amylase ◽  
Potential Biomarker ◽  
Salivary Cortisol Level ◽  
Healthy Control ◽  
Cortisol Levels

BACKGROUND: Hypothalamic-pituitary-adrenal axis and its end product cortisol have been extensively investigated in patients with depressive disorders for many years. Recently, salivary alpha-amylase (sAA) had emerged as a new biomarker with non-invasive and more convenience protocol for measuring sympathetic activity which were also associated with depression. Selective Serotonin Reuptake Inhibitor is antidepressant drug extensively used to treat depression. AIM: The aim of this study was to determine whether decrease of sAA and salivary cortisol levels could be observed in subjects with depression who were treated by fluoxetine. METHODS: The total subjects were 25 depressed subjects and ten healthy controls. sAA was examined before therapy, and after 2, 4, and 6 weeks of fluoxetine administration using a portable cocorometer. Salivary cortisol was examined before therapy, after 4 and 6 weeks of fluoxetine administration with Elisa method. The therapeutic effect was assessed with Hamilton Depression Rating Scale (HDRS). RESULTS: sAA and cortisol levels were significantly decreased after fluoxetine administration (p < 0.001), followed by at least 50% reduction of HDRS scores after 6 weeks of fluoxetine administration. Levels of sAA and cortisol were higher in the depression group than in the healthy control. CONCLUSIONS: Measurement of sAA levels can be used as a potential biomarker of therapeutic response in depressed patients in addition to salivary cortisol.

SALIVARY CORTISOL LEVELS TO ESTABLISH A CAUSE AND EFFECT RELATIONSHIP IN PROGRESSION OF ORAL POTENTIALLY MALIGNANT DISORDERS

2021 ◽  
pp. 4-7
Author(s):  
Shefali Shefali ◽  
Saurabh Juneja ◽  
Anshi Jain ◽  
Devi Charan Shetty ◽  
Nikita Gulati
Keyword(s):  
Salivary Cortisol ◽  
Study Groups ◽  
Oral Potentially Malignant Disorders ◽  
Group Iii ◽  
Complex Interactions ◽  
Group I ◽  
Potentially Malignant Disorders ◽  
Potentially Malignant ◽  
Elisa Technique ◽  
Cortisol Levels

The progression and development of OPMDs is inuenced by a multitude of factors which include complex interactions between physiological, psychological, behavioral and social factors. The persistent activation of HPA axis through tobacco usage probably impairs immune response and has a role in progression of OPMDs. The quantication of salivary cortisol facilitates the assessment of nicotine impact on the oral mucosa and in the progression of OPMDs. This study was undertaken to estimate the salivary cortisol levels in the OPMDs with and without habits thereby signifying the importance of salivary cortisol in the causation of disease or as an effective biomarker for disease progression during the pathogenetic process of the disease. Salivary cortisol levels were estimated by ELISA technique in 29 cases of differing grades of oral potentially malignant disorders consisting of individuals with habit and lesions (Group I), 32 cases of individuals having habit without lesions (Group II) and 3 cases of individuals having lesion without habit (Group III) and 8 cases with neither habit nor lesions (Group IV). Salivary cortisol levels were correlated within the different study groups and were analyzed using SPSS (version 20). Salivary cortisol levels were raised in group I as compared to all other groups. Clarity in the present study has been achieved that salivary cortisol levels can be researched to the causation of the disease as an important step forward. This study could open up newer avenues in understanding the pathogenetic mechanisms in Oral Potentially malignant disorders.

scholarly journals Electroneurographic parameters in patients with metabolic syndrome

2014 ◽  
Vol 4 (1) ◽  
pp. 45-49
Author(s):  
Suljo Kunić ◽  
Emir Tupković ◽  
Mediha Nišić ◽  
Semiha Salihović
Keyword(s):  
Diabetes Mellitus ◽  
Metabolic Syndrome ◽  
Glycemic Control ◽  
Action Potential ◽  
Median Nerve ◽  
Ulnar Nerve ◽  
Sensory Nerve ◽  
Group I ◽  
Patients With Diabetes ◽  
Group Ii

Introduction: The aim of this study was to measure electroneurographic (ENG) parameters of the median and ulnar nerve in patients with metabolic syndrome and to determine whether the large imbalance in glycemic control came to neuropathic changes to the template.Methods: The study included 100 patients with metabolic syndrome diagnosed according to the criteria of the National Cholesterol Education Program - Adult Treatment Panel III (NCEP-ATP III). The patients were divided into two groups. Group I – patients with normal glycemic control and Group II - patients with diabetes mellitus for up to five years. We measured sensory conductive velocity (SCV), the amplitude of sensory nerve action potential (SNAP), motor conductive velocity (MCV), terminal motor latency (TML) and compose muscle action potential after distal stimulation (CMAP-I) and after proximal stimulation (CMAP-II) for the ulnar and median nerve.Results: Sensory and motor parameters in Group II were amended to neuropathic pattern compared to Group I. There were significant differences in: SNAP amplitude for all tested nerves, SCV values for both left and right median and ulnar nerve; MCV and TML for left median nerve; MCV, TML and CMAP-I for right median nerve area; MCV and TML for left ulnar nerve; MCV, CMAP-I and CMAP-II for right ulnar nerve area.Conclusion: Patients with metabolic syndrome and diabetes mellitus duration of five years have the significant changes in sensory and motor peripheral nerves. Neuropathic changes are possible in patients with metabolic syndrome and normal glycemic control.

scholarly journals Parameters of cardiac hemodynamics in patients with diabetes mellitus with impairment of renal function

HYPERTENSION ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 5-10
Author(s):  
I.I. Topchiy ◽  
O.N. Kirienko ◽  
P.S. Semyonovykh ◽  
D.O. Kirienko ◽  
O.I. Tsygankov ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Renal Function ◽  
Structural Parameters ◽  
Left Ventricular ◽  
Control Group ◽  
Group I ◽  
Patients With Diabetes

Currently, diabetes mellitus (DM) is a complex global problem, which is increasing every year. So, in 2019, diabetes mellitus was detected in 463 million adults (from 20 to 79 years old) in the world. And the main cause of death in patients with diabetes mellitus is cardiovascular complications. The study was aimed investigate the features of functional and structural changes in the heart in patients with type 2 diabetes mellitus and nephropathy. A total of 98 patients with type 2 diabetes mellitus were examined, out of which 78 patients had diabetic nephropathy (DN) of varying severity. The control group consisted of 20 healthy patients. After a clinical examination, depending on the state of renal function, all patients were divided into the following groups: group I — patients with type 2 diabetes mellitus without signs of nephropathy (n = 36), group II — patients with type 2 diabetes mellitus with normal glomerular filtration rate (GFR) and albuminuria (n = 33), group III — patients with type 2 diabetes mellitus with decreased GFR and albuminuria (n = 29). To study changes in hemodynamics and structural parameters of the heart, patients underwent transthoracic echocardiography on an ULTIMA PA ultrasound machine (Radmir, Ukraine) using a sectoral phased transducer with a frequency range of 2–3 MHz according to the standard technique based on the recommendations of the American Echocardiographic Society. To determine indexing indicators, the patients underwent anthropometric measurements. Patients with DN and albuminuria and decreased GFR showed an increase in the linear dimensions of the heart in comparison with controls and patients without signs of nephropathy. With DN, patients have a significant increase in left ventricular mass and a significant increase in the detection rate of left ventricular hypertrophy up to 91.3 % in patients with albuminuria and preserved renal function and up to 100 % with a decrease in GFR.

scholarly journals Salivary cortisol levels: the importance of clown doctors to reduce stress

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Flávia G. Saliba ◽  
Natanael S. Adiwardana ◽  
Eliane U. Uehara ◽  
Renata N. Silvestre ◽  
Victor V. Leite ◽  
...  
Keyword(s):  
Medical School ◽  
Visual Analog Scale ◽  
Salivary Cortisol ◽  
Healing Process ◽  
Saliva Sample ◽  
Stress Factor ◽  
Hospitalized Children ◽  
Analog Scale ◽  
Pediatric Ward ◽  
Cortisol Levels

This study was designed to correlate entertainment of clown-doctors (CD) activities on hospitalized children and aphysiological biomarker. For this purpose we collected saliva samples and verified children satisfaction with these activities by using a visual analog scale (VAS). Children from 6 to 7 years-old, with diagnosis of any acute pathology, interned in the Pediatric Ward of the Botucatu Medical School Hospital (São Paulo, Brazil) were interviewed. Two groups were taken into consideration: lunchCD and dinnerCD. The following protocol was applied in each group (lunch and dinner): collection of the first saliva sample and presentation of VAS prior to CD activities, followed by collection of a second saliva sample and another VAS assessment after CD activities. The salivary cortisol was reduced in both groups comparing the first saliva sample. The satisfaction of the intervention was evident for lunchCD. The CD intervention is effective in decreasing an important physiological biomarker of stress factor, cortisol, in hospitalized children, been effective for the healing process.

scholarly journals The Prevalence of Nondiabetic Renal Diseases in Patients with Diabetes Mellitus in the University Hospital of Ribeirão Preto, São Paulo

2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Diego Agra Souza ◽  
Gyl Eanes Barros Silva ◽  
Igor Lima Fernandes ◽  
Dyego José Araújo de Brito ◽  
Monique Pereira Rêgo Muniz ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Membranous Glomerulonephritis ◽  
Sao Paulo ◽  
Group Iv ◽  
University Hospital ◽  
Renal Diseases ◽  
Group I ◽  
Patients With Diabetes ◽  
The University ◽  
Time Since Diagnosis

Objective. To evaluate the prevalence of nondiabetic renal diseases (NDRDs) in renal biopsies of patients with diabetes mellitus (DM) in the University Hospital of Ribeirão Preto, São Paulo. Research Design and Methods. We conducted a retrospective study including kidney biopsies performed in diabetic patients between 1987 and 2013. We evaluated 79 biopsies during this period. The primary variable was the prevalence of NDRD in patients with DM. The secondary variables were the presence of systemic arterial hypertension (SAH), hematuria, time since diagnosis of DM, serum creatinine, and proteinuria levels. The cases were divided into the following groups: isolated diabetic nephropathy (DN—group I), isolated nondiabetic renal diseases (NDRD—group II), associated NDRD/DN (group III), and associated NDRD+NDRD/DN (group IV). Results. Most of the patients (58.22%) presented only alterations arising from DN. NDRDs were present in 41.77% of the patients. Membranous glomerulonephritis (30.3%) and IgA nephropathy (24.24%) were the most prevalent NDRDs. We found no differences between female and male patients with NDRD when assessing the secondary variables. A time since diagnosis of five years or less revealed a statistical difference (p=0.0005) in the comparison between the isolated DN (group I) and the NDRD+NDRD/DN (group IV). The other secondary variables were not significant in the comparison of the groups. Conclusions. We concluded that the prevalence of NDRD is 41.77%. Membranous glomerulonephritis was the most prevalent NDRD in our study. We also conclude that the probability of the presence of NDRD with or without concomitant DN is greater for patients who had biopsies with a time since diagnosis of five years or less. A time since diagnosis of ten years or more does not allow the exclusion of the presence of NDRD.

scholarly journals Sympathetic Nervous System Activity in Patients with Metabolic Syndrome, Type 2 Diabetes Mellitus and Chronic Kidney Disease. Role of Renalase and Alpha Amylase

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Hernández LE ◽  
◽  
Hernández GP ◽  
Aguilar CA ◽  
ánchez DCV ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Metabolic Syndrome ◽  
Type 2 Diabetes Mellitus ◽  
Alpha Amylase ◽  
Control Group ◽  
Salivary Alpha Amylase ◽  
Sympathetic Hyperactivity ◽  
Esrd Patients

Aim: To evaluate serum renalase and salivary alpha amylase concentrations and their relationship with plasma catecholamine levels in patients with Metabolic Syndrome (MS), Type 2 Diabetes Mellitus (T2DM), and End-Stage Renal Disease (ESRD). Method: In cross-sectional study 163 patients were enrolled; 43 patients with MS, 93 withT2DM, 13 with ESRD, and 14 healthy subjects (control group). All patients had their ascription to the UMAA/UMF No. 75, in Morelia, Michoacan, Mexico. Glucose, creatinine, cholesterol, triglycerides, HDL, and renalase were measurement in serum, and Dopamine (DA), Epinephrine (E) and Norepinephrine (NE) in plasma. An aliquot of saliva was collected for alpha amylase determination. Results: High plasma concentrations of DA, E and NE (p<0.0001) was founded in T2DM and ESRD patients. Renalase was lower in ESRD compared to MS and T2DM patients (p<0.0001). High concentrations of alpha amylase were found in MS, T2DM, and ESRD patients in comparison with control group (p<0.0001). Catecholamines correlated positively with alpha amylase and diabetes evolution. Conclusion: Sympathetic hyperactivity in MS, T2DM and ESRD patients was founded. Renalase could be proposed as biomarker of renal function and salivary alpha amylase as sympathetic hyperactivity. Additional studies are required to evaluate the pathophysiological mechanisms involved of SNS in CKD development.

Comparative Evaluation of Salivary Cortisol Levels and Peripheral Blood Neutrophil Functions in Chronic Periodontitis Patients with and without Stress – A Case Control Study

2021 ◽  
Vol 13 (2) ◽  
pp. 23-29
Author(s):  
Sachin nil ◽  
Savita AM ◽  
Sunil S ◽  
Tejashwini MA ◽  
Archana Naik ◽  
...  
Keyword(s):  
Salivary Cortisol ◽  
Chronic Periodontitis ◽  
Control Group ◽  
Stress Profile ◽  
Clinical Attachment Loss ◽  
Peripheral Blood Neutrophil ◽  
Published Evidence ◽  
Group I ◽  
Healthy Control ◽  
Cortisol Levels

Background and aim: Development of chronic periodontitis (CP) is related to the presence of periopathogenic bacteria and several other conditions including stress. In the successful management of chronic periodontitis, early detection of psychological stress plays a crucial role. Salivary cortisol is found to be associated with stress, however not many published evidence exists suggesting the association of stress and salivary cortisol levels in chronic periodontitis. Hence, this study was carried out with the aim to assess salivary cortisol levels and neutrophil functions in chronic periodontitis patients with and without stress. Materials and methods: Cortisol levels were determined using ELISA method from saliva samples collected, analysis of neutrophil functions such as chemotaxis, phagocytosis and intracellular killing was done using nitro blue tetrazolium (NBT) assay, and stress was assessed using Derogatis Stress Profile scale in 60 participants. Study participants were categorized into two groups based on their periodontal condition, Group I: Healthy control (n=30) and Group II: Chronic periodontitis (CP) (n=30) with a probing depth of ≥ 4mm and Clinical attachment loss (CAL) AL >1mm. Results: The difference in mean stress score of the chronic periodontitis group (0.52 ± 0.12) and healthy control group (0.46 ± 0.08) was statistically significant (P < 0.05). Salivary cortisol levels in the healthy (7.77 ± 5.48) and chronic periodontitis (8.77 ± 8.75) groups did not differ significantly. Neutrophil functions defects in the CP group (32.70 ± 12.15) and healthy group (20.33 ± 10.17) differed in a statistically significant manner. Conclusion: Statistically significant differences were observed in the stress levels, salivary cortisol levels and neutrophil functions between healthy and CP subjects with and without stress, thus demonstrating a possible link between CP and stress.

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