Incidence, Gross Morphology, Histopathology and Immunohistochemistry of Canine Mammary Tumors

The aim of present investigation was to study the gross morphology and incidence of canine mammary tumors (CMTs) based on age, sex, breed, reproductive status and location along with histopathological classification and immunohistochemical characteristics. A total of 56 CMTs samples were collected from 49 cases of dogs. Gross morphology was studied in 26 cases of canine mammary tumors. For histopathological classification, samples were fixed in 10% formalin, embedded in paraffin and sections (5 μm thick) obtained from each was stained with HandE stain. Immuno-histochemistry was carried out by using p63 antibody to confirm the histopathological types of CMTs. Malignant tumors and benign tumors were mostly observed in older dogs. Among 9 breeds affected, the highest incidence was recorded in a German shepherd. Caudal abdominal pair was most commonly affected. Most of the cases were observed in intact female dogs, except for one male dog. The tumors were oval and round in shape with 30–2000 g weight, soft to hard in consistency and grayish white cut surface. Out of 56 CMTs, the highest incidence was found of malignant neoplasms (36/56, 64.28%), followed by benign neoplasms (10/56, 17.85%) and non-neoplastic proliferation hyperplasia/dysplasia (10/56, 17.85%). Complex carcinoma, carcinoma, and malignant myoepithelioma and malignant myoepithelioma were confirmed by p63 antibody. In these neoplasms, myoepithelial cells showed strong immunoreactivity with p63.Development of nitrogen use efficient (NUE) rice varieties is inevitable for sustainability of environmental friendly and economical agricultural practices. Several management practices are being studied for increasing efficiency of spatial and temporal inputs of N under National Innovations for Climate Resilient Agriculture (NICRA). Attempts are being made to develop NUE rice varieties with multidisciplinary approach and conventional selection along with mapping and next generation sequencing strategies. Around 800 rice genotypes were characterized under low and recommended nitrogen for two consecutive seasons and the promising donors further evaluated to identify consistent NUE rice genotypes. Several mapping populations were developed using the NUE donors and popular rice varieties. QTL/genomic regions were identified for yield under low N using biparental and association mapping. Using minimum marker set of 50 rice SSR markers, 12 genomic regions were identified for yield and yield associated traits under low nitrogen. Several promising recombinants of yield and NUE were identified and these breeding lines were evaluated under multi-locations and stable performers were identified under AICRIP Trial - Evaluation of Radiation and Nitrogen use efficient promising rice genotypes -Plant Physiology during Kharif 2016, 2017 and 2018. As nitrogen is the building block of biomass, an optimum N is required for realizing the yield. The strategy should be to maximise uptake and improve utilization, so that remobilization of N to yield is achieved under low N.

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An Immunohistochemical Study on the Expression of Sex Steroid Receptors in Canine Mammary Tumors

ISRN Veterinary Science ◽

10.5402/2012/378607 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Leena Rajathy Port Louis ◽

Khub Chandra Varshney ◽

Madhavan Gopalakrishnan Nair

Keyword(s):

Estrogen Receptor ◽

Epithelial Cells ◽

Estrogen Receptor Alpha ◽

Malignant Tumors ◽

Steroid Receptors ◽

Estrogen Receptor Beta ◽

Smooth Muscles ◽

Mammary Tumors ◽

Benign Tumors ◽

Canine Mammary Tumors

Steroid hormones are found to play a major role in the genesis and progression of mammary tumors. The aim of this study was to immunohistochemically detect the presence of estrogen receptor alpha (ERα), estrogen receptor beta (ERβ), and progesterone receptor (PR) and also to study the association between these markers in 29 cases of benign (11) and malignant (18) canine mammary tumors. ERα immunostaining was noticed in only one case of carcinosarcoma specifically in the nuclei of epithelial and a few myoepithelial cells. ERβ immunostaining was noticed in the nuclei and cytoplasm of epithelial cells and smooth muscles lining the blood vessels. Immunoexpression of ERβ was 82% in benign tumors and 78% in malignant tumors. PR immunostaining was expressed in the nuclei of epithelial cells in both benign and malignant tumors. Among the 15 PR+ cases, 6 (55%) were of benign type, and 9 (50%) were of malignant type. The most common group of hormone receptor was the ERα−/PR+/ERβ+ (46%) in benign tumors and ERα−/PR−/ERβ+ (38%) in malignant tumors. Although there was no significant association between ERα and PR with ERβ, the findings indicated that ERβ was consistently expressed in both benign and malignant tumors, irrespective of ERα and PR status.

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Canine Mammary Tumors: Comparison of Classification and Grading Methods in a Survival Study

Veterinary Pathology ◽

10.1177/0300985818806968 ◽

2018 ◽

Vol 56 (2) ◽

pp. 208-219 ◽

Cited By ~ 13

Author(s):

Ana Canadas ◽

Miguel França ◽

Cristina Pereira ◽

Raquel Vilaça ◽

Hugo Vilhena ◽

...

Keyword(s):

Prognostic Factors ◽

Malignant Tumors ◽

Mammary Tumors ◽

Classification Systems ◽

World Health ◽

Benign Tumors ◽

Histological Subtype ◽

Canine Mammary Tumors ◽

Nottingham Grade ◽

Health Organization

Histopathology remains the cornerstone for diagnosing canine mammary tumors (CMTs). Recently, 2 classification systems (the World Health Organization [WHO] classification of 1999 and the proposal of 2011) and 2 grading methods based on the human Nottingham grade have been used by pathologists. Despite some evidence that the histological subtype and grade are prognostic factors, there is no comprehensive comparative study of these classification and grading systems in the same series of CMTs. In this study, the 2 classifications and the 2 grading methods were simultaneously applied to a cohort of 134 female dogs with CMTs. In 85 animals with malignant tumors, univariable and multivariable survival analyses were performed. Using the 2 systems, the proportion of benign (161/305, 53%) and malignant (144/305, 47%) tumors was similar and no significant differences existed in categorization of benign tumors. However, the 2011 classification subdivided malignant tumors in more categories—namely, those classified as complex, solid, and tubulopapillary carcinomas by the WHO system. Histological subtype according to both systems was significantly associated with survival. Carcinomas arising in benign tumors, complex carcinomas, and mixed carcinomas were associated with a better prognosis. In contrast, carcinosarcomas and comedocarcinomas had a high risk of tumor-related death. Slight differences existed between the 2 grading methods, and grade was related to survival only in univariable analysis. In this cohort, age, completeness of surgical margins, and 2 index formulas adapted from human breast cancer studies (including tumor size, grade, and vascular/lymph node invasion) were independent prognostic factors.

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Immunohistochemical Analysis of Na+/K+-ATPase and Bone Morphogenetic Protein-2 Expressions in Both Parenchymal and Stromal Cells from Benign and Malignant Canine Mammary Tumors

10.21203/rs.3.rs-431238/v1 ◽

2021 ◽

Author(s):

ozlem ozmen

Keyword(s):

Stromal Cells ◽

Malignant Tumors ◽

Breast Tumors ◽

Mammary Tumors ◽

Bone Morphogenetic Protein 2 ◽

Mammary Tissue ◽

Benign Tumors ◽

Ion Pump ◽

Human Breast ◽

Canine Mammary Tumors

Abstract Canine mammary tumors are the most common type of dog tumor, and they are similar to human breast tumors. Na+/K+-ATPase is a common plasma membrane ion pump with important physiological and pathophysiological functions. In mammary tumors, the tumor microenvironment was composed of a heterogeneous population of tumor cells and nearby endogenous stromal cells. Bone morphogenetic proteins (BMPs) regulate fetal development, tissue homeostasis and differentiation, and a variety of cellular functions. The purpose of this study is to examine the immunohistochemical expression of Na+/K+-ATPase and BMP-2 in tumoral and stromal cells from benign and malignant canine mammary tumors. In this study, ten benign and ten malignant mammary tumors from the archives of the Department of Pathology were used, with five normal breast tissues serving as controls. The results of the revealed that tumors had higher levels of Na+/K+-ATPase and BMP-2 expressions than normal mammary tissue. While both markers were expressed negatively or mildly in benign tumors, they increased significantly in malignant tumors. Both Na+/K+-ATPase and BMP-2 are expressed by tumoral and stromal cells in canine mammary tumors. When compared to compared to BMP-2, Na+/K+-ATPase expression was found to be more severe. This study found that Na+/K+-ATPase and BMP-2 can be used as markers of malignancy in canine mammary tumors and that stromal cells also play an important role in tumor progression. These findings also indicated that Na+/K+-ATPase and BMP-2 may be used for early diagnosis or as a potential target for treatment of canine and human breast tumors in the future.

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Mast cells and angiogenesis in canine mammary tumor

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/s0102-09352010000600008 ◽

2010 ◽

Vol 62 (6) ◽

pp. 1348-1351 ◽

Cited By ~ 6

Author(s):

G.E Lavalle ◽

A.C Bertagnolli ◽

W.L.F Tavares ◽

M.A.N.D Ferreira ◽

G.D Cassali

Keyword(s):

Mast Cells ◽

Microvessel Density ◽

Malignant Tumors ◽

Mammary Tumors ◽

Blue Staining ◽

Benign Tumors ◽

Toluidine Blue Staining ◽

Canine Mammary Tumors ◽

Significant Difference ◽

Malignant And Benign Tumors

The correlation between microvessel density and mast cells density in canine mammary tumors was studied. Sixty-five samples of canine mammary tumors, being 24 benign and 41 malignant, were analyzed. The routine Toluidine Blue staining method was used to assess the mast cells. To evaluate angiogenesis, the immunohistochemical expression of CD31 was assessed. There was no significant difference in either mast cells (P=0.44) or microvessel density (P=0.77) between malignant and benign tumors. A positive correlation was observed between microvessel density and mast cells (r=0.39; P=0.011) in malignant tumors. These results suggest that mast cells may play a role in canine mammary malignant tumors development, promoting angiogenesis, similar to some tumors described in the human species

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Number of Nucleoli and Nucleolar Organizer Regions per Nucleus and Nucleolus — Prognostic Value in Canine Mammary Tumors

Veterinary Pathology ◽

10.1177/030098589603300507 ◽

1996 ◽

Vol 33 (5) ◽

pp. 527-532 ◽

Cited By ~ 10

Author(s):

M. BratuliĆ ◽

Ž GrabareviĆ ◽

B. ArtukoviĆ ◽

D. Capak

Keyword(s):

Malignant Tumors ◽

Organizer Region ◽

Nucleolar Organizer Region ◽

Nucleolar Organizer ◽

Staining Technique ◽

Mammary Tumors ◽

World Health ◽

Nucleolar Organizer Regions ◽

Canine Mammary Tumors ◽

Significant Difference

Twenty-eight canine mammary tumors were evaluated for histopathologic classification as recommended by the World Health Organization and silver-binding nucleolar organizer region (AgNOR) and nucleolus counts. Samples of surgically excised tumors and tumors taken at necropsy were fixed in neutral formalin, embedded in paraffin, and cut into 1-3-μm-thick sections. Two sections were taken from each tumor: one was stained with hematoxylin and eosin and the other was treated with the silver staining technique for the demonstration of AgNORs. After histopathologic classification, the number of nucleoli and the number of AgNORs/nucleus and AgNORs/nucleolus were determined. Statistical analysis (Student's t-test) showed a significant difference in the mean number of nucleoli ( P < 0.005), mean number of AgNORs/nucleolus ( P < 0.001), and mean number of AgNORs/nucleus ( P < 0.005) between benign and malignant canine mammary tumors. There was no significant differences between metastatic and nonmetastatic malignant tumors.

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A Statistical Analysis of Risk Factors and Biological Behavior in Canine Mammary Tumors: A Multicenter Study

Animals ◽

10.3390/ani10091687 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1687

Author(s):

Giovanni P. Burrai ◽

Andrea Gabrieli ◽

Valentina Moccia ◽

Valentina Zappulli ◽

Ilaria Porcellato ◽

...

Keyword(s):

Machine Learning ◽

Risk Factors ◽

Statistical Analysis ◽

Multicenter Study ◽

Malignant Tumors ◽

Mammary Tumors ◽

Supervised Machine Learning ◽

Biological Behavior ◽

Clinical Staging ◽

Canine Mammary Tumors

Canine mammary tumors (CMTs) represent a serious issue in worldwide veterinary practice and several risk factors are variably implicated in the biology of CMTs. The present study examines the relationship between risk factors and histological diagnosis of a large CMT dataset from three academic institutions by classical statistical analysis and supervised machine learning methods. Epidemiological, clinical, and histopathological data of 1866 CMTs were included. Dogs with malignant tumors were significantly older than dogs with benign tumors (9.6 versus 8.7 years, p < 0.001). Malignant tumors were significantly larger than benign counterparts (2.69 versus 1.7 cm, p < 0.001). Interestingly, 18% of malignant tumors were smaller than 1 cm in diameter, providing compelling evidence that the size of the tumor should be reconsidered during the assessment of the TNM-WHO clinical staging. The application of the logistic regression and the machine learning model identified the age and the tumor’s size as the best predictors with an overall diagnostic accuracy of 0.63, suggesting that these risk factors are sufficient but not exhaustive indicators of the malignancy of CMTs. This multicenter study increases the general knowledge of the main epidemiologica-clinical risk factors involved in the onset of CMTs and paves the way for further investigations of these factors in association with CMTs and in the application of machine learning technology.

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Spontaneous Tumors and Non-Neoplastic Proliferative Lesions in Pet Degus (Octodon degus)

Veterinary Sciences ◽

10.3390/vetsci7010032 ◽

2020 ◽

Vol 7 (1) ◽

pp. 32

Author(s):

Tanja Švara ◽

Mitja Gombač ◽

Alessandro Poli ◽

Jožko Račnik ◽

Marko Zadravec

Keyword(s):

Malignant Tumors ◽

Single Case ◽

Case Reports ◽

Hepatocellular Adenoma ◽

Benign Tumors ◽

Malignant Neoplasms ◽

Octodon Degus ◽

Hind Limbs ◽

Neoplastic Lesion ◽

Veterinary Faculty

In recent years, degus (Octodon degus), rodents native to South America, have been becoming increasingly popular as pet animals. Data about neoplastic diseases in this species are still sparse and mainly limited to single-case reports. The aim of this study was to present neoplastic and non-neoplastic proliferative changes in 16/100 pet degus examined at the Veterinary Faculty University of Ljubljana from 2010 to 2015 and to describe the clinic-pathological features of these lesions. Twenty different lesions of the integumentary, musculoskeletal, genitourinary and gastrointestinal systems were diagnosed: amongst these were 13 malignant tumors, six benign tumors, and one non-neoplastic lesion. Cutaneous fibrosarcoma was the most common tumor (7/16 degus). It was detected more often in females (6/7 degus) and lesions were located mainly in hind limbs. The gastrointestinal tract was frequently affected, namely with two malignant neoplasms - an intestinal lymphoma and a mesenteric mesothelioma, four benign tumors – two biliary cystadenomas, an oral squamous papilloma and a hepatocellular adenoma, and a single non-neoplastic proliferative lesion. In one animal, two organic systems were involved in neoplastic lesions.

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Retrospective Study of Mammary Lesions in Bitches - Uberlândia, MG, Brazil

Acta Scientiae Veterinariae ◽

10.22456/1679-9216.80788 ◽

2017 ◽

Vol 45 (1) ◽

pp. 8

Author(s):

Mariana Batista Andrade ◽

Ednaldo Carvalho Guimarães ◽

Arlinda Flores Coleto ◽

Nicolle Pereira Soares ◽

Alessandra Aparecida Medeiros-Ronchi

Keyword(s):

Retrospective Study ◽

Tumor Size ◽

Hormone Receptors ◽

Malignant Tumors ◽

Histological Grade ◽

Mammary Tumors ◽

Mammary Glands ◽

Malignant Neoplasms ◽

The Mean ◽

Inguinal Glands

Background: Mammary tumors are a type of neoplasia that are most commonly found in female dogs and are mostly malignant. The aim of this study, performed in the Laboratory of Veterinary Pathology of the Federal University of Uberlândia (LVP-FUU) from 2004 to 2014, was to determine the prevalence of mammary tumors in bitches and to verify the relationship between the epidemiological factors (age and breed) and clinicopathological aspects (ulceration, tumor size, and malignancy) in the occurrence of tumors.Materials, Methods & Results: A retrospective study was carried out using histopathological information retrieved from the LPV-UFU database. We collected the information on age and breed of female dogs, as well as about the location, macroscopic aspects, and histological diagnosis of mammary lesions. Only female dogs were considered for this study; a total of 911 histopathological protocols (with only one diagnosis) were analyzed along with 36 protocols that presented more than one diagnosis of mammary tumor. The age of animals ranged from one to 20 years, and the mean age was 9.99 years. The most affected breeds of dogs were: Cross breed (39.56% - 288/728) and Poodle (20.19% - 147/728). The inguinal glands were most affected by the malignant tumors (P < 0.05). A prevalence of tumors bigger than 5 cm in diameter (T3) was observed in the elderly animals (P = 0.0154) and in the inguinal mammary glands (P = 0.044). Simple carcinoma was the most frequent histological type.Discussion: Research shows that more than 40% of the tumors in bitches are located in the mammary glands, emphasizing the importance of this type of neoplasia in female dogs. Mammary tumors develop more frequently in the middle-aged and elderly bitches, with the highest occurrence being in the age range of 8 and 10 years, corroborating our observation in the present study that the mean age of bitches was 9.99 years. In this survey, a higher incidence was observed in mongrel bitches compared to that in the Poodle breed. Some authors affirm that there is no racial predisposition for the occurrence of this pathology; however, a compilation of data suggests a predisposition of at least 10 breeds, with the involvement of an as yet unidentified genetic component. Of these, six breeds (Poodle, Cocker Spaniel, Pointer, Maltese, Yorkshire Terrier, and Dachshund) were found to be predisposed to mammary tumors in this study. The percentage (49.23%) of malignant tumors found in the inguinal glands is consistent with the findings reported in literature, and might be associated with a greater amount of parenchyma, abundance of hormone receptors in these glands, and vascularization provided by the caudal superficial epigastric artery and vulvar branches of external pudendal artery. Tumor size is considered to be a prognostic factor and tumors ≤ 3 cm in diameter (T1) have a better prognosis. Consequently, the prevalence of tumors ≥ 5 cm in diameter (T3) in elderly animals is probably related to malignancy of the lesions, because tumors usually progress to a worse histological grade with time. The higher occurrence of T3 in inguinal glands might be related to the abundance of parenchyma and/or hormonal receptors in them. As in the present study, data from literature refer to the superiority of malignant histological types, with prevalence varying between 68 and 91%. When prolonged, the time between the onset of tumor and clinical evaluation may be a determinant in the progression from benign to malignant tumors. Among the malignant neoplasms, simple carcinoma was observed to be prevalent, followed by mixed tumors with carcinoma, in agreement with the results of several studies. It is concluded that mammary tumors are more prevalent in older mongrel dogs and Poodle. Attention should be paid to inguinal mammary tumors, because these are mainly malignant.

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Risk Factors of Malignant Neoplasms of Female Genital Organs (Review of Literature)

Journal of New Medical Technologies ◽

10.12737/3326 ◽

2014 ◽

Vol 21 (1) ◽

pp. 115-121 ◽

Cited By ~ 2

Author(s):

Сулейманова ◽

N. Suleymanova

Keyword(s):

Malignant Tumors ◽

Pathological Process ◽

The Body ◽

Morbidity Rate ◽

Benign Tumors ◽

Malignant Neoplasms ◽

Scientific Review ◽

Papillomavirus Infection ◽

Hormone Homeostasis ◽

Female Genital

The scientific review discusses the correlation between the MN morbidity rate of the female genitalia and the factors of the external and internal environment of the body: genetic (hereditary) and environmental (exogenous and endogenous). The author notes that the significance of the factors in the development of oncological process is different depending on the form and localizations of malignant tumors. Identified genes are responsible for the appearance of hereditary ovarian cancer (however, the genes of predisposition to cancer of body and cervix of the uterus don’t identified so far). The role of human papillomavirus infection (in the genesis of pre-cancerous lesions and cervical cancer) in hormone homeostasis due to functional and anatomical changes in the hypothalamic-pituitary-ovarian system (in formation of cancer of the womb and ovaries) is proved, including the background processes and pre-cancerous changes in the occurrence of all forms of genital cancer. A number of researchers consider benign tumors as an intermediate in the pathological process changes that lead with time under the influence of certain factors to be precancerous and malignant transformation. Significant fluctuations in the frequency of malignant tumors of female genital organs in different ethnic groups of the population are scientifically confirmed. Correlation frequency of cancer of the genitalia in women with age, and state of the immune system are noted.

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