scholarly journals Antidiabetic effect of Centella asiatica extract (whole plant) in streptozotocin nicotinamide-induced diabetic rats

2019 ◽  
Vol 6 (1) ◽  
pp. 14 ◽  
Author(s):  
Arwin Muhlishoh ◽  
Brian Wasita ◽  
Adi Magna Patriado Nuhriawangsa
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Food Intake ◽  
Centella Asiatica ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Whole Plant ◽  
One Way Anova

<p><strong>ABSTRAK</strong></p><p><strong>Latar belakang:</strong> Pegagan (<em>Centella asiatica</em>) telah digunakan untuk pengobatan diabetes mellitus. Namun dosis efek anti diabetes ini belum diteliti.</p><p><strong>Tujuan:</strong> Untuk menguji pengaruh variasi dosis ekstrak pegagan (seluruh bagian tanaman) terhadap kadar gula darah, asupan makanan, dan berat badan pada tikus model diabetes mellitus yang diinduksi Streptozotocin Nicotinamide.</p><p><strong>Metode:</strong> Tiga puluh enam tikus Wistar jantan dibagi menjadi enam kelompok perlakuan berbeda: kontrol negatif, kontrol positif, kontrol obat (metformin 45 mg/KgBB/hari), ekstrak pegagan 300 mg/KgBB/hari, 600 mg/KgBB/hari, 1200 mg / KgBB / hari. Pemberian ekstrak pegagan (seluruh bagian tanaman) dan metformin dilakukan selama 28 hari. Kadar gula darah dianalisis menggunakan Glucose Oxidase Phenol Aminoantipyrina Peroxidase (GOD-PAP) sebelum dan sesudah perlakuan. Berat badan dan asupan makanan diukur setiap satu minggu. Data dianalisis menggunakan One Way ANOVA dengan tingkat kepercayaan 95%.</p><p><strong>Hasil:</strong> Pemberian variasi dosis ekstrak pegagan dan metformin secara signifikan menurunkan kadar gula darah, meningkatkan berat badan dan memperbaiki asupan makanan (p = 0,00). Kadar gula darah dan asupan makanan pada kelompok ekstrak pegagan (seluruh bagian tanaman) dosis tinggi (1200 mg / KgBB / hari) lebih baik dibanding kelompok dosis rendah (300 mg / KgBB / hari) dan kelompok dosis sedang (600 mg / hari), dan memiliki efek yang sama dengan tikus yang diobati dengan metformin 45 mg / KgBB / hari.</p><p><strong>Kesimpulan:</strong> Dosis tinggi ekstrak pegagan (1200 mg/KgBB/hari) memiliki efek anti-diabetes yang lebih baik dibanding dosis lain, dan memiliki efek yang sama dengan kontrol obat.</p><p><strong>KATA KUNCI:</strong> gula darah, diabetes, pegagan, Streptozotocin nicotinamide</p><p><strong>ABSTRACT</strong></p><p><strong> Background:</strong> <em>Centella </em>asiatica has been used for healing diabetes mellitus. The dosage of this anti diabetic effect was yet to be explored.</p><p><strong>Objectives:</strong> To examine the effect of the variation in the dosage of C. asiatica extract (whole plant) on blood glucose levels, food intake, and body weight in Streptozotocin Nicotinamide induced diabetic rats.</p><p><strong>Methods:</strong> Thirty-six male Wistar rat were divided into six different groups of treatments: negative control, positive control, medication control (metformin 45 mg/KgBW/day), extract of<em> C. asiatica</em> 300 mg/KgBW/day, 600 mg/KgBW/day, 1200 mg/KgBW/day. Treatments of <em>C. asiatic</em>a extract (whole plant) and metformin was done for 28 days. Blood glucose was analyzed using the Glucose Oxidase Phenol Aminoantipyrina Peroxidase (GOD-PAP) before and after treatment. Body weight and food intake were measured every one week. The data were analyzed using One Way ANOVA with 95% confidence level.</p><p><strong>Results:</strong> The administration of the variation in the dosage of <em>C. asiatica</em> extract and metformin significantly decrease blood glucose levels, increase body weight and improve food intake (p =0.00). Blood glucose level and food intake among high dosage of <em>C. asiatica</em> extract (whole plant) group (1200 mg/KgBW/day) is better than the low dosage group (300 mg/KgBW/day) and moderate dosage group (600 mg/KgBW/ day), and have the same effect with the rats treated with metformin 45 mg/KgBW/day.</p><p><strong>Conclusion:</strong> High dosage of <em>C. asiatica</em> extract (1200 mg/KgBW/day) had a better anti-diabetic effe than other dosages, and had the same effect with the medication control.</p><p><strong>KEYWORDS:</strong> blood glucose,<em> Centella asiatica,</em> diabetes, nicotinamide, Streptozotocin</p>

Evaluation of Glucose and Lipid Lowering Activity of Arganimide A in Normal and Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (4) ◽  
pp. 503-510 ◽  
Author(s):  
Mohamed Eddouks ◽  
Farid Khallouki ◽  
Robert W. Owen ◽  
Morad Hebi ◽  
Remy Burcelin
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lowering Activity

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

scholarly journals Pharmaceutical hit of anti type 2 Diabetes mellitus on the phenolic extract of Malaka (Phyllanthus emblica L.) flesh

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Musri Musman ◽  
Mauli Zakia ◽  
Ratu Fazlia Inda Rahmayani ◽  
Erlidawati Erlidawati ◽  
Safrida Safrida
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Body Weight ◽  
Blood Glucose ◽  
Phyllanthus Emblica ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Phenolic Extract

Abstract Background Ethnobotany knowledge in a community has shaped local wisdom in utilizing plants to treat diseases, such as the use of Malaka (Phyllanthus emblica) flesh to treat type 2 diabetes. This study presented evidence that the phenolic extract of the Malaka flesh could reduce blood sugar levels in the diabetic induced rats. Methods The phenolic extract of the P. emblica was administrated to the glucose-induced rats of the Wistar strain Rattus norvegicus for 14 days of treatment where the Metformin was used as a positive control. The data generated were analyzed by the two-way ANOVA Software related to the blood glucose level and by SAS Software related to the histopathological studies at a significant 95% confidence. Results The phenolic extract with concentrations of 100 and 200 mg/kg body weight could reduce blood glucose levels in diabetic rats. The post hoc Dunnet test showed that the administration of the extract to the rats with a concentration of 100 mg/kg body weight demonstrated a very significant decrease in blood glucose levels and repaired damaged cells better than administering the extract at a concentration of 200 mg/kg weight body. Conclusion The evidence indicated that the phenolic extract of the Malaka flesh can be utilized as anti type 2 Diabetes mellitus without damaging other organs.

scholarly journals HYPOGLYCEMIC ACTIVITY OF A NOVEL POLYHERBAL FORMULATION IN STREPTOZOTOCIN-INDUCED DIABETIC RATS: A THERAPEUTIC STUDY

2019 ◽  
pp. 218-223
Author(s):  
PULAK MAJUMDER ◽  
PARIDHAVI M
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Beta Cells ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Polyherbal Formulation ◽  
Blood Glucose Levels ◽  
Therapeutic Study ◽  
Glutamyl Transferase

Objective: The concept of the synergistic effect of poly-herbalism was as old as medicine history. Present novel polyherbal formulation (PHF) composed of five different herbs. The present investigation aimed to evaluate the synergistic therapeutic hypoglycemic potential of PHF against streptozotocin (STZ) (60 mg/kg b.w, ip)-induced diabetic rats. Methods: For this therapeutic study, the dose was framed orally once a day to the test objects after STZ dosing at 500 mg/kg/5 ml dosage levels for 21 days. The transformation of body weight and blood glucose level was examined, and the histopathological changes of beta cells of the pancreas, cellular architectures of liver and kidney were also perceived after scarification of the objects. Results: The outcomes were compared to that of glibenclamide (5 mg/kg) treated group. Declines of body weight and blood glucose levels were perceived in STZ-induced diabetic animals very significantly (p<0.01 or p<0.05). However, these diabetic changes were significantly (p<0.01 or p<0.05) decreased in PHF-dosing groups revealed more encouraging effects compared to that of glibenclamide. In the other hand, various liver function and enzymes test (creatinine, urea, total bilirubin, total albumin, alkaline phosphatase, gamma-glutamyl transferase, aspartate transaminases, and alanine transaminases) and lipid profile (total cholesterol, triglycerides, high-density lipoprotein cholesterol, total protein, low-density lipoprotein [LDL], and very LDL) studies strongly indicate the potential action of this novel formulation. Conclusions: It is deliberated that PHF has the favorable effect to normalize the blood glucose levels, and also rejuvenation and reproduction of beta cells lead a better futuristic ant diabetic therapy for diabetic management.

scholarly journals Antidiabetic activity of curry leaves Murraya koenigii on glucose level, kidney and islets of langerhans in streptozotocin induced diabetes in rats

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Imad M. Al-Ani ◽  
Rahajoe I. Santosa ◽  
Muhammad H. Yankuzo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Murraya Koenigii ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Curry Leaves ◽  
Aqueous Leaf Extract

Introduction: This study examined the antihyperglycemic effect of curry leaves, Murraya koenigii “MK” aqueous extract, and to examine its possible protective effects on the Islets of Langerhans and kidneys in streptozotocin (STZ) induced diabetic rats.  Methods: Thirty healthy adult male Sprague Dawley rats were randomized into five groups (n=6); normal control, normal treated with “MK” control, diabetic control (non-treated with “MK”), diabetic treated with 200mg/kg MK aqueous leaf extract and diabetic treated with 400mg/kg MK aqueous leaf extract. Blood glucose levels and body weight were monitored. The animals were sacrificed on the 30th day; the kidney and pancreatic tissues were processed for histological studies. Results: The diabetic control group significantly (p<0.001) showed considerable loss of body weight and increase in blood glucose levels and degeneration of the glomeruli and renal convoluted tubules and atrophied islets with disintegration of β-cells. Treatment of diabetic rats with aqueous extract showed significant (p<0.001) improvement in blood glucose levels and body weight gain.  The MK extract also caused an improvement in tissue injury induced by STZ injection in the kidney and endocrine pancreas.  Conclusions: These findings highlighted the beneficial effects of MK aqueous extract against cellular oxidative damage in STZ-induced diabetic rats.

Potent Antihyperglycemic Effects of an Endemic Plant from Morocco (Matthiola maroccana Coss.) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Nadia Lahrach ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Endemic Plant ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The study aims to evaluate the antidiabetic effect of Matthiola maroccana (Coss.). Background: Matthiola maroccana (Coss.) belongs to Brassicaceae family is an endemic plant from Morocco. Objective: The objective of the study was to evaluate the effect of its aqueous extract on blood glucose levels in normal and diabetic rats. Method: Single dose (6 hours) and daily oral administration for seven days of the Aerial Part Aqueous Extract (A.P.A.E) of Matthiola maroccana (Coss.) (M. maroccana) at a dose of 20 mg/kg body weight on blood glucose levels both in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, body weight, oral glucose tolerance test, liver histopathological examination, phytochemical screening, and in vitro antioxidant activity of A.P.A.E were performed in this study. Results: The results showed that M. maroccana A.P.A.E exerts potent hypoglycemic and antihyperglycemic effects in normal and STZ-induced diabetic rats (p<0.0001). Also, it was able to restore body weight in diabetic rats (p<0.05). Furthermore, the aqueous extract has been shown to regenerate hepatic tissues in diabetic rats. In addition, A.P.A.E revealed the presence of several phytochemical constituents (polyphenols, flavonoids, tannins, saponins, alkaloids, sterols and terpenoids), and possessed an antioxidant activity. Conclusion: In conclusion, our findings showed that A.P.A.E of M. maroccana (A.P.A.E MM) possesses a significant antihyperglycemic and hypoglycemic activity.

Effects of Aqueous Root Extract of Jatropha curcas on Hyperglycaemic and Haematological Indices in Alloxan-induced Diabetic Rats

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
R A Aladodo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Cell ◽  
Jatropha Curcas ◽  
Diabetic Rats ◽  
Haematological Parameters ◽  
Root Extract ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Aqueous Root Extract

Hypoglycaemic potentials of aqueous root extract of Jatropha curcas (250 and 450 mg/kg body weight of rats) was investigated in alloxan-induced diabetic rats. Effect of the extract on some haematological parameters was also investigated. Thirty albino rats (Rattus norvegicus) of mean weight 165.0 ± 2.50 g were randomly selected into normal and diabetic groups, each with six rats. Diabetic treated rats were placed on 14.2 mg/kg body weight glucophage (reference drug), 250 mg/kg and 450 mg/kg body weight of extract. All treatments were administered orally, once daily for fifteen days. The blood glucose levels were monitored and the haematological parameters like haemoglobin (Hgb), red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), percentage lymphocytes (LYM) and neutrophils (NEUT) were also analyzed. A significant (p<0.05) reduction in fasting Blood Glucose Levels (BGL) was observed in alloxan-induced diabetic rats treated with the extract. There was a significant (p<0.05) reduction in Hgb, PCV, RBC and WBC and its differentials of the diabetic untreated rats while administration of glucophage, 250 and 450 mg/kg body weight of the aqueous extract (Jc250 and Jc450) to diabetic rats significantly (p<0.05) increase all these parameters. These results suggested that the aqueous root extract of Jatropha curcas possesses hypoglycaemic activity and also improved the aberrations in the blood parameters of alloxan-induced diabetic rat.

The effect of Syzygium cumini (L.) skeels on post-prandial blood glucose levels in non-diabetic rats and rats with streptozotocin-induced diabetes mellitus

1997 ◽  
Vol 56 (3) ◽  
pp. 209-213 ◽  
Author(s):  
Claudio Coimbra Teixeira ◽  
Luciano Pilla Pinto ◽  
Felix Henrique Paim Kessler ◽  
Lia Knijnik ◽  
Cristiano Pilla Pinto ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Diabetic Rats ◽  
Syzygium Cumini ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Streptozotocin Induced Diabetes

scholarly journals Antidiabetic and antihyperlipidemic activities of extracts of Barleria cuspidata Heyne ex Nees on streptozotocin induced diabetic rats

2021 ◽  
Vol 12 (1) ◽  
pp. 643-652
Author(s):  
Manohar Reddy ◽  
Raja Sundararajan
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Glycosylated Hemoglobin ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Serum Triglycerides ◽  
Methanol Extracts ◽  
Glucose Levels ◽  
Blood Glucose Levels

Traditionally, Barleria cuspidata Heyne ex Nees is utilized for antidiabetic action with the absence of logical investigation. Thus, the current examination was attempted to explore for its antidiabetic and antihyperlipidemic movement in streptozotocin instigated diabetic animal models. Blood glucose levels were estimated in normoglycemic rats at initial, 60th and 120th minutes intervals and in glucose feed hyperglycemic rats at initial, 30, 60, 90 and 120 minutes after a solitary portion of streptozotocin at 55 mg/kg body weight intraperitoneal were made diabetic in albino rats. Blood glucose levels were estimated at week by week spans after everyday administration of chloroform and methanol extracts of Barleria cuspidata at dosages of 250 and 500 mg/kg body weight. Other biochemical boundaries of serum triglycerides, cholesterol, HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, total protein, albumin, globulin, uric acid, creatinine, urea, transaminases, alkaline phosphatase, alanine aminotransaminase, insulin and glycosylated hemoglobin were likewise estimated toward the finish of the investigation. Chloroform and methanol extracts of Barleria cuspidata by an oral organization for 21 days altogether (P<0.001) decreases the elevated blood glucose extents in diabetic rats whereas in normoglycemic rats it doesn't adjust the blood glucose amounts altogether and in glucose feed hyperglycemic rats significantly decreases the raised blood glucose levels. Likewise, the chloroform and methanol extracts of Barleria cuspidata improved other biochemical boundaries related to diabetes. Moreover, the extracts of Barleria cuspidata favourable affect the histopathological changes of pancreas in streptozotocin initiated diabetic rats. Delayed consequences legitimize the traditional utilization of Barleria cuspidata for its anti-diabetic action.

scholarly journals Effect of Sorghum-tigernut Ibyer (A Traditional Gruel) on the Fasting Blood Glucose Levels of Alloxan-induced Diabetic Rats

2019 ◽  
pp. 260-268
Author(s):  
Shalem Shiekuma ◽  
Moses Ukeyima ◽  
Msendoo, Janet Ahuah ◽  
Idoko Blessing ◽  
Terzungwe Tughgba
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Saline Water ◽  
Fasting Blood Glucose ◽  
Sensory Attributes ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Sorghum Flour ◽  
Blood Glucose Levels ◽  
Alloxan Monohydrate

Background: There is growing interest in the use of natural foods in the management of chronic diseases like diabetes. Ibyer is a fibre rich gruel consumed amongst the Tiv people of Benue State made from whole sorghum or millet flours. Aim: The aim of the study was to evaluate the effect of sorghum-tigernut ibyer on the fasting blood glucose levels and body weight of alloxan monohydrate-induced diabetic rats. Methods: Sorghum flour (SF) and tigernut flour (TNF) were blended at different proportions (100:00; 90:10; 80:20; 70:30) for the purpose of ibyer production. The flour samples were subjected to proximate analysis using standard analytical procedures, the sensory attributes of ibyer produced from the different flour samples was evaluated on a 9-point hedonic scale. Thirty (30) male Wistar rats (100–180 g body weight) were grouped into five (1-5) each group containing six rats. They were induced with diabetes by injecting them with 150ml/kg of body weight with alloxan monohydrate dissolved in saline water (0.9% NaCl) except for group 1. Blood samples were collected from the tail of the rats, prior to induction, 48hrs after induction and 72 hrs after three days of continuous feeding with test diet. Fasting blood glucose was measured using a standard glucometer and test strips. Results: The sensory attributes indicated that ibyer produced from the flour samples were generally acceptable. Fasting blood glucose levels after 72 hrs of feeding were found to be lowered more in groups giving flours with a higher proportion of Tigernut. Conclusion: The results indicated that sorghum-tigernut ibyer exerted hypoglycaemic effect on the experimental animals.

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