scholarly journals Application of Fasudil in the Treatment of Contrast-Induced Acute Renal Insufficiency Based on Computerized Tomography Intelligent information monitoring (Preprint)

2020 ◽  
Author(s):  
Xiuming Zhang ◽  
Tao Liu ◽  
Chunhua Tian ◽  
Lucien Herbert
Keyword(s):  
Renal Insufficiency ◽  
Renal Disease ◽  
Contrast Agent ◽  
Computerized Tomography ◽  
Disease Model ◽  
Control Group ◽  
Model Group ◽  
High Group ◽  
Acute Renal Insufficiency ◽  
Intelligent Information

BACKGROUND Background: Contrast-induced acute kidney injury (CI-AKI) is one of the main causes of hospital-acquired acute kidney failure. At present, the exact pathogenesis of CI-AKI is not yet clear. However, the contrast agent reduces renal blood flow and filtration rate, which is its direct toxic effect on renal tubules. Fasudil can relax vascular smooth muscle and act on the kidney, which has a very obvious effect on the expansion of the renal arterioles; also, by adjusting the vascular tone of the microcirculation before and after the glomerulus, it can regulate renal hemodynamics. OBJECTIVE Objective: The objective was to explore the application of Fasudil in the treatment of contrast-induced acute renal insufficiency based on computerized tomography intelligent information monitoring, and explore the application of single-photon emission computerized tomography (SPECT) in the diagnosis of renal insufficiency. METHODS Methods: 32 BALB/C mice were randomly divided into 4 groups, i.e., the renal disease model group (the model group), the renal disease model plus Fasudil high dosage group (the Fasudil high group), the renal disease model plus Fasudil low dosage group (the Fasudil low group), and the control group. After the mice models were grouped, they were banned from drinking water for 12 h. Mice in the model group, the Fasudil high group, and the Fasudil low group were injected with iodixanol injections at a dosage of 4 gI/kg through caudal veins. Mice in the control group were injected with the same dosage of saline. Respectively at 12 h and 2 h before contrast agent injection and 4 h after contrast agent injection, mice in the two Fasudil groups were administered with Fasudil through intraperitoneal injections. The dosage for the Fasudil high group was 10 mg/kg, and the dosage for the Fasudil low group was 10 mg/kg. At the same time nodes, mice in the model group were administered with the same dosage of saline. The histopathological changes in renal tissues, the variations in renal functions, as well as the changes in renal hemodynamics, were observed. RESULTS Results: (1) Compared with the control group, in the model group, the serum creatinine (Cr) and blood urea nitrogen (BUN), as well as the urine N-acetyl-β-D-glucosaminidase (NAG) levels, increased significantly (P<0.05). Compared with the model group, in the Fasudil high group, the serum Cr and BUN, as well as the urine NAG levels, increased significantly (P<0.05). (2) After high-dose fasudil intervention treatment, the destruction of kidney structure in mice was significantly reduced, and the condition of renal tubular epithelial cells swelling was improved. (3) Compared with the control group, in the model group, the RABF values of mice decreased significantly (P<0.05). In the high Fasudil group, after the intervention of high dosed Fasudil, the RABF values of mice increased significantly (P<0.05). CONCLUSIONS Conclusion: The therapy of high-dosage Fasudil for acute renal damages of mice induced by contrast agents could effectively reduce the serum Cr, serum BUN, and urine NAG levels and act as an anti-apoptotic and anti-infective agent. In addition, Fasudil could resist the oxidative stress, thereby improving the contrast agent-induced vasoconstriction responses. The special functional imaging of SPECT can objectively reflect the function of living organs. CLINICALTRIAL

scholarly journals Effect of amber powder on endometrial ultrastructure and MAPK pathway in endometriosis model rats

2021 ◽  
Vol 18 (9) ◽  
pp. 1845-1851
Author(s):  
Xiaona Ma ◽  
Yanhui Wu ◽  
Bingbing Li ◽  
Zheng Wang ◽  
Xiujuan Zhu ◽  
...  
Keyword(s):  
Dose Group ◽  
Mapk Pathway ◽  
Morphological Changes ◽  
Disease Model ◽  
Control Group ◽  
High Dose ◽  
Model Group ◽  
Sprague Dawley ◽  
Blank Group ◽  
Endometrial Cells

Purpose: To explore the therapeutic role of amber powder in endometriosis by investigating its effect on endometrial ultrastructure, ERK1/2, p38MAPK, and NF-κB mRNA pathways and CSRC/EFR/ERK1/2 proteins. Methods: Sprague Dawley (SD) rats were randomly divided into blank group, disease model group (untreated), amber powder high-dose group, amber powder medium-dose group, amber powder lowdose group and danazol group. Morphological changes in endometrial cells were studied using transmission electron microscopy. The expression of ERK1/2, p38MAPK, and NF-κB mRNA in endometrial tissues of each group was determined using quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry was utilized for the measurement of C-SRC/EFR/ERK1/2 pathway protein expression. Results: The endometriosis rats treated with a high-, medium- and low-dose amber powder showed a decrease in the volume of ectopic lesions, compared with the untreated disease model group. The expressions of ERK1/2, p38MAPK, NF-κB mRNA, and C-SRC/EFR/ERK1/2 protein were higher in the eutopic and ectopic endometrial tissues in untreated disease group than those in normal control group. Moreover, treatment of endometriosis rats with amber powder revealed a reduction in the expressions of ERK1/2, p38MAPK, NF-κB mRNA and C-SRC/EFR/ERK1/2 proteins in eutopic and ectopic endometrium tissues. Conclusion: Amber powder reduces ectopic lesions and slows down the development of endometriosis, probably via inhibition of MAPK pathway genes in eutopic and ectopic endometrial tissues.

The Treatment of Acute Renal Insufficiency

1950 ◽  
Vol 34 (2) ◽  
pp. 509-523 ◽  
Author(s):  
I. Snapper
Keyword(s):  
Renal Insufficiency ◽  
Acute Renal Insufficiency

MR Contrast Agent Poses Danger in Renal Disease

2007 ◽  
Vol 41 (2) ◽  
pp. 54-55
Author(s):  
BRUCE K. DIXON
Keyword(s):  
Renal Disease ◽  
Contrast Agent

Educational technology for patient with chronic renal disease: integrative review / Tecnologia educativa para paciente com doença renal crônica: revisão integrativa / Tecnología educativa voltada al paciente con enfermedad renal crónica: revisión integradora

2018 ◽  
Vol 7 (2) ◽  
pp. 72
Author(s):  
Cristiano Batista Gonçalves
Keyword(s):  
Health Promotion ◽  
Educational Technology ◽  
Renal Insufficiency ◽  
Renal Disease ◽  
Chronic Renal Disease ◽  
Integrative Review ◽  
Tecnología Educativa

Objetivo: analisar a produção científica nacional e internacional sobre tecnologias educativas voltadas à promoção da saúde do paciente com doença renal crônica. Metodologia: trata-se de uma revisão integrativa da literatura; utilizou-se a questão norteadora: Quais as evidências disponíveis na literatura científica sobre tecnologias educativas voltadas à promoção da saúde do paciente com doença renal crônica?; não houve imposição de limite de tempo, e utilizou-se as bases de dados SciELO, MEDLINE, LILACS e CINAHL, com cruzamento dos seguintes descritores: renal insufficiency, educational technology, health promotion. Resultados: incluíram-se seis estudos nesta revisão e elencou-se quatro categorias sobre as tecnologias educativas: software para computador ou dispositivo móvel, material impresso, programa educacional via telefone e website. Conclusão: diferentes tecnologias educativas construídas evidenciam a preocupação dos profissionais da saúde em promover a longitudinalidade do cuidado e integralidade da saúde aos indivíduos com doença renal crônica, como também lhes instiga a tornarem-se protagonistas no cuidado da sua saúde.Descritores: Insuficiência Renal Crônica. Tecnologia Educacional. Promoção da Saúde.

scholarly journals Study on the Mechanism of Shugan Xiaozhi Fang on Cells with Non-alcoholic Fatty Liver Disease

2019 ◽  
Vol 17 (1) ◽  
pp. 1328-1338
Author(s):  
Yufeng Xing ◽  
Chuantao Zhang ◽  
Fenfen Zhai ◽  
Tianran Zhou ◽  
Xiang Cui ◽  
...  
Keyword(s):  
Liver Disease ◽  
Fatty Liver Disease ◽  
Dose Group ◽  
Control Group ◽  
High Dose ◽  
Model Group ◽  
Alcoholic Fatty Liver ◽  
Oil Red O Staining ◽  
Alcoholic Fatty Liver Disease ◽  
Oil Red O

AbstractCells with non-alcoholic fatty liver disease (NAFLD) were studied to determine the mechanism of liver deficiency via the AdipoR2-PPARa pathway. NAFLD cells were randomly divided into a normal control group, blank control group, model group, low dose group, medium dose group, and high dose group. The NAFLD models were established by incubating the cells with linoleic acid (LA) and palmitic acid (PA) (2:1) for 24 h. The test groups were incubated with different doses of Shugan Xiaozhi Fang extract. The pathological changes in cells that accumulated lipids were detected by Oil Red O staining. Malondialdehyde (MDA) and triglyceride (TG) levels were measured. The apoptosis of cells was evaluated by flow cytometry. The levels of AdipoR2, PPARa, CD36, acyl-CoA mRNA, and protein were confirmed by RT- PCR and Western blot. The results of the Oil Red O staining demonstrated that the NAFLD cell model was successfully established. Compared with the model group, the levels of TG and MDA in the groups that received low, medium, and high doses of Shugan Xiaozhi were significantly lower (P<0.01), and a dose effect was evident. In addition, the expression of AdipoR2, PPARa, CD36, acyl-CoA protein, and mRNA in the Shugan Xiaozhi-treated groups was upregulated. Furthermore, the levels of AdipoR2, PPAR, CD36, acyl-CoA protein, and mRNA in all drug treatment groups that were extracted from L-O2 normal human hepatocytes were significantly upregulated (P<0.01). Moreover, the factor pattern of HepG2 human liver carcinoma cells was similar to that of L-O2. The levels of AdipoR, CD36, acyl-CoA, and AdipoR mRNA in the HepG2 low group were increased (P<0.05). AdipoR, PPAR, CD36, and acyl-CoA protein levels and AdipoR mRNA expression were significantly increased in the intermediate dose group and high dose group (P<0.01). Shugan Xiaozhi Fang attenuates hepatic lipid deposition in NAFLD induced by incubating with LA and PA for 24 h, which is associated with the activation of the AdipoR2-PPARα pathway.

scholarly journals Hepatoprotective effect of galangin on carbon tetrachloride-induced hepatotoxicity via the LKB1/AMPK pathway

2021 ◽  
Vol 19 ◽  
pp. 205873922110008
Author(s):  
Meng Chen ◽  
Xinyan Song ◽  
Jifang Jiang ◽  
Lei Xing ◽  
Pengfei Wang
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Toxicity ◽  
Corn Oil ◽  
Histopathological Examination ◽  
Hepatoprotective Effect ◽  
Control Group ◽  
Group Model ◽  
Protective Effects ◽  
Model Group ◽  
Expression Levels

To investigate the protective effects of galangin on liver toxicity induced by carbon tetrachloride (CCl4) in mice. Mouse hepatotoxicity model was established by intraperitoneal injection (i.p.) of 10 ml/kg body weight CCl4 that diluted with corn oil to a proportion of 1:500 on Kunming mice. The mice were randomly divided into five groups named control group, model group, and 1, 5, and 10 mg/kg galangin group. The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed by ELISA. Liver histopathological examination was observed via optical microscopy. The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and glutathion (GSSG) were analyzed to assess oxidative stress. Finally, western blot assay was carried out to analyse the expression levels of total AMP-activated protein kinase (AMPK), phospho-AMPK (p-AMPK), total liver kinase B1 (LKB1), and phospho-LKB1 (p-LKB1). Compared with the control group, in the model group, the levels of AST, ALT, MDA, and GSSG increased significantly ( p < 0.01); the activity of SOD and GSH decreased significantly ( p < 0.01); and the histopathological examination revealed liver necrosis. However, treatment with galangin (5 and 10 mg/kg) significantly reversed these CCl4-induced liver damage indicators. Furthermore, treatment with galangin (10 mg/kg) significantly increased the p-AMPK and p-LKB1 expression levels ( p < 0.01). This study supports the hepatoprotective effect of galangin against hepatotoxicity, perhaps occurring mainly through the LKB1/AMPK-mediated pathway.

scholarly journals Comparative study on the antituberculous effect and mechanism of the traditional Chinese medicines NiuBeiXiaoHe extract and JieHeWan

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Li-Yao Duan ◽  
Yan Liang ◽  
Wen-Ping Gong ◽  
Yong Xue ◽  
Jie Mi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chinese Medicine ◽  
Treatment Group ◽  
Mechanism Of Action ◽  
Cell Damage ◽  
Control Group ◽  
Therapeutic Effects ◽  
Model Group ◽  
Lung Histopathology ◽  
Th17 Cytokines

Abstract Background The traditional Chinese medicine NiuBeiXiaoHe (NBXH) extract and Chinese medicine preparation JieHeWan (JHW) exhibit anti-tuberculosis effects. The anti- tuberculosis effect of NBXH was compared with that of JHW to elucidate the mechanism of action of NBXH. Methods BALB/c mice aged 6-8 weeks were randomly divided into a normal control group, Tuberculosis (TB) model group, JHW treatment group, and NBXH treatment group. After 3 and 13 weeks of treatment, the therapeutic effect in each group was evaluated by comparing lung histopathology, lung and liver colony counts, the number of spots representing effector T cells secreting IFN-γ in an ELISPOT, and the levels of Th1, Th2, and Th17 cytokines, which were measured by a cytometric bead array (CBA). Mouse RNA samples were subjected to transcriptome sequencing. Results After 13 weeks of treatment, the mean histopathological lesion area of the NBXH group was significantly smaller than that of the TB model group (P < 0.05). Compared with those in the TB model group, the lung colony counts in the JHW and NBXH groups were significantly decreased (P < 0.05), and the IL-2 and IL-4 levels in the NBXH group were significantly increased (P < 0.05). NBXH partly restored significant changes in gene expression caused by Mycobacterium tuberculosis (M. tuberculosis) infection. According to GO and KEGG analyses, the changes in biological process (BP), cell composition (CC) and molecular function (MF) terms and in signaling pathways caused by NBXH and JHW treatment were not completely consistent, but they were mainly related to the immune response and inflammatory response in the mouse TB model. Conclusions NBXH had therapeutic effects similar to those of JHW in improving lung histopathology, reducing lung colony counts, and regulating the levels of cytokines. NBXH restored significant changes in gene expression and repaired cell damage caused by M. tuberculosis infection by regulating immune-related pathways, which clarified the mechanism of action of NBXH.

scholarly journals ICAM-1-carrying targeted nano contrast agent for evaluating inflammatory injury in rabbits with atherosclerosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ping Li ◽  
Lin Jin ◽  
Lan Feng ◽  
Yingchun Wang ◽  
Rong Yang
Keyword(s):  
Contrast Agent ◽  
Outer Membrane ◽  
Signal Intensity ◽  
Vascular Wall ◽  
Control Group ◽  
Imaging Method ◽  
Vascular Perfusion ◽  
Inflammatory Injury ◽  
Modeling Group ◽  
Group B

AbstractTo investigate the feasibility of using ICAM-1-targeted nano ultrasonic contrast to evaluate the degree of inflammatory injury at different stages in the abdominal aorta of rabbits with atherosclerosis (AS). Twenty-five experimental rabbits were assigned to five groups: the control group (A); the week-4 after modeling group (B); the week-8 after modeling group (C); the week-12 after modeling group (D); the week-16 after modeling group (E). All groups were given 2D ultrasonography, conventional ultrasonic contrast (SonoVue), and ICAM-1-targeted nano ultrasonic contrast, respectively. Signal intensity of vascular perfusion was evaluated. Signal intensity of ICAM-1-targeted nano ultrasonic contrast was substantially enhanced and prolonged in the vascular wall of the abdominal bubble aorta increased in B, C, D, and E groups (all P < 0.05). A positive linear correlation between intensity and the expression of ICAM-1 (r = 0.895, P < 0.001). The intensity of outer membrane was enhanced from week 4 to week 12, and both the intima-media membrane and outer membrane were enhanced with double-layer parallel echo at week 16, which was in line with the progression of atherosclerotic plaque inflammatory injury. ICAM-1-targeted nano contrast agent would be possibly a novel non-invasive molecular imaging method for plaque inflammatory injury and site high expression of specific adhesion molecules in early atherosclerotic lesions.

Sign in / Sign up

Export Citation Format

Share Document