scholarly journals Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

2018 ◽  
Vol 22 (1) ◽  
pp. 49 ◽  
Author(s):  
Pratiwi Apridamayanti ◽  
Rarastoeti Pratiwi ◽  
Yekti Asih Purwestri ◽  
Woro Anindito Sri Tunjung ◽  
Rumiyati Rumiyati
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Rice Cultivars ◽  
Black Rice ◽  
Nutrient Contents ◽  
Macro And Micronutrients ◽  
Black Rice Bran

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

Phenolic Profiles and Antioxidant Activity of Black Rice Bran of Different Commercially Available Varieties

2010 ◽  
Vol 58 (13) ◽  
pp. 7580-7587 ◽  
Author(s):  
Ming Wei Zhang ◽  
Rui Feng Zhang ◽  
Fang Xuan Zhang ◽  
Rui Hai Liu
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Black Rice ◽  
Phenolic Profiles ◽  
Black Rice Bran

scholarly journals Encapsulation of Anthocyanins from Black Rice (Oryza Sativa L.) Bran Extract using Chitosan-Alginate Nanoparticles

2017 ◽  
Vol 6 (3) ◽  
pp. 40 ◽  
Author(s):  
Rodel M. Bulatao ◽  
John Paulo A. Samin ◽  
Joel R. Salazar ◽  
Juvy J. Monserate
Keyword(s):  
Particle Size ◽  
Encapsulation Efficiency ◽  
Oryza Sativa L ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Scavenging Activity ◽  
Black Rice ◽  
Rice Varieties ◽  
Freeze Dried

This study was conducted to extract and encapsulate anthocyanins from black rice bran using chitosan-alginate nanoparticles. Ten black rice varieties were screened for the anthocyanin content and the variety with the highest anthocyanins was used for the encapsulation. The anthocyanins were extracted by defatting the bran with n-hexane and soaking it with 85% acidified ethanol. The crude anthocyanin extract (CAE) was freeze-dried at -110°C for 48 h and then encapsulated in chitosan-alginate nanoparticles using two processes: ionic pre-gelation and polyelectrolyte complex formation. The mass ratio of chitosan and alginate polymers used in this study was 100:10. The treatments applied were as follows: T0-0 mg CAE, T1-10 mg CAE, T2-20 mg CAE, and T3-30 mg CAE. The resulting capsules were characterized in terms of chemical properties, surface morphology, particle size, polydispersive index, encapsulation efficiency, and 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity. Screening of rice samples indicated that Ominio bran had the highest anthocyanin content (36.11 mg/g). Anthocyanins were successfully encapsulated in the matrix as shown by the Scanning Electron Microscopy images and Fourier Transform Infrared spectra of the anthocyanin-loaded chitosan-alginate nanoparticles. Among the different concentrations of CAE, T3 had the highest encapsulation efficiency (68.9%) and antioxidant scavenging activity (38.3%) while T1 and T2 had the lowest. Ascending particle size was observed for T0 (358.5 nm), T3 (467.9 nm), T1 (572.3 nm), and T2 (635.9 nm). All anthocyanin-loaded capsules were found to be of nano-size (<1000 nm). The study concluded that chitosan-alginate nanoparticles can be a good encapsulating material for anthocyanin.

scholarly journals The Effect of Ethanolic Extract of Black and White Rice Bran (Oryza sativa L.) on Cancer Cells

2017 ◽  
Vol 21 (1) ◽  
pp. 63
Author(s):  
Rizal Maarif Rukmana ◽  
Nyoman Puniawati Soesilo ◽  
Rumiyati Rumiyati ◽  
Rarastoeti Pratiwi
Keyword(s):  
Cancer Cells ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
Probit Analysis ◽  
Rice Cultivars ◽  
Chromatography Method ◽  
White Rice ◽  
Wide Range ◽  
Black Rice Bran

Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source ofphytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activitiesof the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and‘IR­64’ (white rice) on cancer cells and to determine the compounds groups of those extracts. First step,rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphomacancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure thecytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics,flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed.The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 valuesof ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cellswere 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 onVero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. Theethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids.However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxicactivities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similarkinds of organic compounds groups but vary in the Rf values.

Hepatoprotective and antioxidant activity of anthocyanins in black rice bran on carbon tetrachloride-induced liver injury in mice

2013 ◽  
Vol 5 (4) ◽  
pp. 1705-1713 ◽  
Author(s):  
Fangli Hou ◽  
Ruifen Zhang ◽  
Mingwei Zhang ◽  
Dongxiao Su ◽  
Zhencheng Wei ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Rice Bran ◽  
Black Rice ◽  
Black Rice Bran

scholarly journals Gastroprotective Activities of Ethanol Extract of Black Rice Bran (Oryza sativa L.) in Rats

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3812
Author(s):  
Peerachit Tonchaiyaphum ◽  
Warangkana Arpornchayanon ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Pornsiri Pitchakarn ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Water Immersion ◽  
Rice Variety ◽  
Ethanol Extract ◽  
Gastric Ulcers ◽  
Black Rice ◽  
Black Rice Bran

Black rice is a type of rice in the Oryza sativa L. species. There are numerous reports regarding the pharmacological actions of black rice bran, but scientific evidence on its gastroprotection is limited. This study aimed to evaluate the gastroprotective activities of black rice bran ethanol extract (BRB) from the Thai black rice variety Hom Nil (O. sativa L. indica) as well as its mechanisms of action, acute oral toxicity in rats, and phytochemical screening. Rat models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress were used. After pretreatment with 200, 400, and 800 mg/kg of BRB in test groups, BRB at 800 mg/kg significantly inhibited the formation of gastric ulcers in all gastric ulcer models, and this inhibition seemed to be dose dependent in an indomethacin-induced gastric ulcer model. BRB could not normalize the amount of gastric wall mucus, reduce gastric volume and total acidity, or increase gastric pH. Although BRB could not increase NO levels in gastric tissue, the tissue MDA levels could be normalized with DPPH radical scavenging activity. These results confirm the gastroprotective activities of BRB with a possible mechanism of action via antioxidant activity. The major phytochemical components of BRB comprise carotenoid derivatives with the presence of phenolic compounds. These components may be responsible for the gastroprotective activities of BRB. The 2000 mg/kg dose of oral BRB showed no acute toxicity in rats and confirmed, in part, the safe uses of BRB.

scholarly journals Gambaran aktivitas antioksidan pada ekstrak beras hitam (Oryza sativa L.) kultivar Pare Ambo Sulawesi Selatan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Raven G. Katiho ◽  
Shirley E. S. Kawengian ◽  
Nelly Mayulu
Keyword(s):  
Antioxidant Activity ◽  
Oryza Sativa L ◽  
Total Content ◽  
Free Radical Scavengers ◽  
Rice Cultivars ◽  
Black Rice ◽  
South Sulawesi ◽  
Total Antioxidant ◽  
Good Potential

Abstract: The objective of this research is to determine the antioxidant activity of the extracts of black rice cultivars Pare Ambo, South Sulawesi. Black rice Pare Ambo cultivars, South Sulawesi extracted with 70% ethanol by maceration. After that, the extract tested antioxidant activity using DPPH with concentration 50, 100, 200, 400, 500 ppm, and methods FRAP for the determination of total content of antioxidants at concentrations 600, 700, 800, 900, 1000 ppm. Research results using DPPH antioxidant test showed a concentration of 50 ppm has an activity that is the lowest free-radical scavengers 22% and 400 ppm had a prophylactic activity of free radicals highest 84,60%. While FRAP method showed a concentration of 600 ppm had the lowest total antioxidant that is 177,64 mmol/g and a concentration of 1000 ppm has the highest total antioxidant that is 272,94 mmol/g. Results of this research concluded that the extract of black rice cultivars Pare Ambo, South Sulawesi has a good potential antioxidant activity.Keywords: black rice, extract, antioxidants, DPPH, FRAPAbstrak: Tujuan penelitian ini adalah untuk menentukan aktivitas antioksidan dari ekstrak beras hitam kultivar Pare Ambo, Sulawesi Selatan. Beras hitam kultivar Pare Ambo, Sulawesi Selatan diekstrak dengan pelarut etanol 70% dengan cara maserasi. Setelah itu, ekstrak diuji aktivitas antioksidan menggunakan metode DPPH dengan konsentrasi 50, 100, 200, 400, 500 ppm, dan metode FRAP untuk penentuan kandungan total antioksidan dengan konsentrasi 600, 700, 800, 900, 1000 ppm. Hasil penelitian pengujian antioksidan menggunakan metode DPPH menunjukkan konsentrasi 50 ppm memiliki aktivitas penangkal radikal bebas terendah yaitu 22% dan konsentrasi 400 ppm memiliki aktivitas penangkal radikal bebas tertinggi yaitu 84,60%. Sedangkan metode FRAP menunjukkan konsentrasi 600 ppm memiliki total antioksidan paling rendah yaitu 177,64 mmol/g dan konsentrasi 1000 ppm memiliki total antioksidan paling tinggi yaitu 272,94 mmol/g. Hasil penelitian ini menyimpulkan bahwa ekstrak beras hitam kultivar Pare Ambo, Sulawesi Selatan memiliki potensi aktivitas antioksidan yang baik.Kata kunci: beras hitam, ekstrak, antioksidan, DPPH, FRAP

scholarly journals Antioxidant activity and phytocemical analysis from black rice bran

2019 ◽  
Vol 335 ◽  
pp. 012019
Author(s):  
Amirotul Muniroh ◽  
Slamet Budijanto ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Black Rice ◽  
Black Rice Bran
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