scholarly journals Gambaran aktivitas antioksidan pada ekstrak beras hitam (Oryza sativa L.) kultivar Pare Ambo Sulawesi Selatan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Raven G. Katiho ◽  
Shirley E. S. Kawengian ◽  
Nelly Mayulu
Keyword(s):  
Antioxidant Activity ◽  
Oryza Sativa L ◽  
Total Content ◽  
Free Radical Scavengers ◽  
Rice Cultivars ◽  
Black Rice ◽  
South Sulawesi ◽  
Total Antioxidant ◽  
Good Potential

Abstract: The objective of this research is to determine the antioxidant activity of the extracts of black rice cultivars Pare Ambo, South Sulawesi. Black rice Pare Ambo cultivars, South Sulawesi extracted with 70% ethanol by maceration. After that, the extract tested antioxidant activity using DPPH with concentration 50, 100, 200, 400, 500 ppm, and methods FRAP for the determination of total content of antioxidants at concentrations 600, 700, 800, 900, 1000 ppm. Research results using DPPH antioxidant test showed a concentration of 50 ppm has an activity that is the lowest free-radical scavengers 22% and 400 ppm had a prophylactic activity of free radicals highest 84,60%. While FRAP method showed a concentration of 600 ppm had the lowest total antioxidant that is 177,64 mmol/g and a concentration of 1000 ppm has the highest total antioxidant that is 272,94 mmol/g. Results of this research concluded that the extract of black rice cultivars Pare Ambo, South Sulawesi has a good potential antioxidant activity.Keywords: black rice, extract, antioxidants, DPPH, FRAPAbstrak: Tujuan penelitian ini adalah untuk menentukan aktivitas antioksidan dari ekstrak beras hitam kultivar Pare Ambo, Sulawesi Selatan. Beras hitam kultivar Pare Ambo, Sulawesi Selatan diekstrak dengan pelarut etanol 70% dengan cara maserasi. Setelah itu, ekstrak diuji aktivitas antioksidan menggunakan metode DPPH dengan konsentrasi 50, 100, 200, 400, 500 ppm, dan metode FRAP untuk penentuan kandungan total antioksidan dengan konsentrasi 600, 700, 800, 900, 1000 ppm. Hasil penelitian pengujian antioksidan menggunakan metode DPPH menunjukkan konsentrasi 50 ppm memiliki aktivitas penangkal radikal bebas terendah yaitu 22% dan konsentrasi 400 ppm memiliki aktivitas penangkal radikal bebas tertinggi yaitu 84,60%. Sedangkan metode FRAP menunjukkan konsentrasi 600 ppm memiliki total antioksidan paling rendah yaitu 177,64 mmol/g dan konsentrasi 1000 ppm memiliki total antioksidan paling tinggi yaitu 272,94 mmol/g. Hasil penelitian ini menyimpulkan bahwa ekstrak beras hitam kultivar Pare Ambo, Sulawesi Selatan memiliki potensi aktivitas antioksidan yang baik.Kata kunci: beras hitam, ekstrak, antioksidan, DPPH, FRAP

scholarly journals Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

2018 ◽  
Vol 22 (1) ◽  
pp. 49 ◽  
Author(s):  
Pratiwi Apridamayanti ◽  
Rarastoeti Pratiwi ◽  
Yekti Asih Purwestri ◽  
Woro Anindito Sri Tunjung ◽  
Rumiyati Rumiyati
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Rice Cultivars ◽  
Black Rice ◽  
Nutrient Contents ◽  
Macro And Micronutrients ◽  
Black Rice Bran

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

scholarly journals Gambaran aktivitas dan stabilitas antioksidan ekstrak beras hitam (Oryza sativa L.) kultivar Enrekang Sulawesi Selatan

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Pricilia Tan ◽  
Nelly Mayulu ◽  
Shirley Kawengian
Keyword(s):  
Antioxidant Activity ◽  
Oryza Sativa ◽  
Nutritional Value ◽  
Oryza Sativa L ◽  
Rice Paddy ◽  
Rice Cultivar ◽  
Black Rice ◽  
South Sulawesi ◽  
The People ◽  
Dpph Method

Abstract: Rice is the main type of food that is very essential to the people of Asia, especially in Indonesia. Color pigments, which are located on the outer layer of black rice paddy, have been reported to contain a mixture of anthocyanin compounds. Anthocyanin compounds act as antioxidants and free radical catchers, which serve to prevent some degenerative diseases. Black rice contains a nutritional value that is superior to other types of rice. It includes high content of protein, vitamins and minerals although there are distinct varieties depending on the location of production. This research has intended to determine the description of the activity and stability of the antioxidant from black rice extract (Oryza sativa L.) cultivars in Enrekang, South Sulawesi at 100 degrees Celsius heating with the periods of 0, 10, 20 and 30 minutes, and the antioxidant activity has been tested using DPPH and FRAP methods. Based on the results of the research using DPPH method, it was discovered that the activity of radical scavengers increased by the effect of the temperature from extracts of the black rice cultivar in Enrekang heated for 0, 10, 20, and 30 minutes at a temperature of 100 ° C and the antioxidant activity of the extracts of the black rice was relatively stable against heating that was tested using FRAP.Keywords: black rice, antioxidants, DPPH, FRAPAbstrak: Beras merupakan jenis pangan utama yang penting bagi masyarakat di Asia, terutama di Indonesia. Pigmen warna yang terletak pada lapisan luar padi dari beras hitam telah dilaporkan mengandung campuran senyawa antosianin, senyawa antosianin berfungsi sebagai antioksidan dan penangkap radikal bebas, sehingga berperan untuk mencegah terjadinya beberapa penyakit degeneratif. Beras hitam memiliki kandungan nilai gizi yang lebih unggul dari beras jenis lainya. Seperti kandungan protein yang tinggi, vitamin dan mineral. Meskipun terdapat perbedaan varietas tergantung pada lokasi produksinya. Penelitian ini bertujuan untuk mengetahui gambaran aktivitas dan stabilitas antioksidan dari ekstrak beras hitam (Oryza sativa L.) kultivar Enrekang, Sulawesi Selatan pada pemanasan 100oc dengan lama pemanasan 0, 10, 20, dan 30 menit dan diuji aktivitas antioksidan menggunakan metode DPPH dan FRAP. Berdasarkan hasil penelitian menggunakan metode DPPH, didapatkan bahwa aktivitas penangkal radikal bebas meningkat dengan efek pemanasan pada ekstrak beras hitam (Oryza sativa L.) kultivar Enrekang Sulawesi Selatan dengan pemanasan 0, 10, 20, dan 30 menit pada suhu 100oC. Dan aktivitas antioksidan pada ekstrak beras hitam relative stabil terhadap pemanasan yang diuji menggunakan metode FRAP.Kata kunci: beras hitam, antioksidan, DPPH, FRAP

scholarly journals Chemical Composition of White Wines Produced from Different Grape Varieties and Wine Regions in Slovakia

2021 ◽  
Vol 11 (22) ◽  
pp. 11059
Author(s):  
Silvia Jakabová ◽  
Martina Fikselová ◽  
Andrea Mendelová ◽  
Michal Ševčík ◽  
Imrich Jakab ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Mean Value ◽  
Total Phenolic ◽  
White Wines ◽  
Tartaric Acids ◽  
Total Antioxidant ◽  
Total Polyphenolic Content ◽  
Wine Region ◽  
Grape Varieties

In this work, chemical parameters such as sugar (glucose and fructose) content, organic acid (total acids, malic and tartaric acids), total phenolic content and the antioxidant activity of 12 white wines (chardonnay, pinot blanc and pinot gris) from various wine regions in Slovakia were studied in order to identify differences among the varieties and wine-growing regions. The wine samples were examined by Fourier-transform infrared spectroscopy (FTIR) and UV-VIS spectrophotometry (for determination of total polyphenolic content (TPC) and total antioxidant activity (TAA)) methods. Content of alcohol ranged between 11.50% and 13.80% with the mean value 12.52%. Mean content of total acids varied between 4.63 ± 0.09 and 6.63 ± 0.05 g.L−1, tartaric acid varied between 1.62 ± 0.09 and 2.93 ± 0.03 g L−1, malic acid was found in the concentrations ranged from 0.07 ± 0.05 and 2.50 ± 0.08 g L−1 and lactic acid was present between 1.53 and 0.01 g L−1. The content of fructose was, in general, higher in the samples from the Južnoslovenská and Nitrianska wine regions and glucose was higher in the Malokarpatská wine region. Chardonnay wines showed the highest content of total polyphenols and the antioxidant activity in the samples ranged from 51.06 ± 027 to 72.53 ± 0.35% inhibition of DPPH. The PCA analysis based on chemical descriptors distinguished the Nitrianska and Stredoslovenská wine regions. According to similarities among the wine samples, four main classes were formed by cluster analysis.

scholarly journals Influence of various factors (temperature, humidity, light) on the chemical composition and antioxidant activity of bisquis

2021 ◽  
pp. 7-14
Author(s):  
A. N. Gulyaeva ◽  
M. S. Voronina ◽  
N. V. Makarova
Keyword(s):  
Antioxidant Activity ◽  
Raw Materials ◽  
Sugar Content ◽  
Total Content ◽  
Total Phenolic ◽  
Black Currant ◽  
Confectionery Products ◽  
High Sugar Content ◽  
Total Antioxidant ◽  
Phenolic Substances

Confectionery - food products, usually with a high sugar content, characterized by high calorie content and digestibility. They are divided into groups: sugar, flour confectionery, chocolate, cocoa. The following types of products are used as the main raw materials for the preparation of confectionery products: flour (wheat, rye, corn, rice, oatmeal, etc.), sugar, honey, fruits and berries, milk and cream, fats, eggs, yeast, starch, cocoa, nuts, food acids, gelling agents, flavoring and aromatic additives, food dyes and baking powder. The high content of carbohydrates, proteins and fats, as well as polyunsaturatedfatty acids and some vitamins determines the significant value of confectionery products. The influence of shelf life on biscuit semi-finished products prepared according to the classical recipe using pomace powder and concentrated juice of black currant and blueberry berries is shown. Methods used in the experimental part: measurement of the total phenolic content using the Folin-Chekelau reagent, the total content of flavonoids and anthocyanins, the level of free radical capture DPPH (2,2-diphenyl-1-picrilhydrazyl), the total antioxidant activity by the FRAP method and the antioxidant activity in the linoleic acid system. It was found that the content of phenolic substances, flavonoids, anthocyanins and antioxidant activity is greatly influenced by high humidity and a constant light source. These indicators are significantly reduced, but when stored in a vacuum, this decrease in indicators can be slowed down.

Changes in the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of their fattening

2020 ◽  
Vol 1 (12) ◽  
pp. 112-118
Author(s):  
O. A. Voronina ◽  
◽  
A. A. Savina ◽  
N. S. Kolesnik ◽  
N. V. Bogolyubova ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Water Soluble ◽  
Laboratory Studies ◽  
Blood Samples ◽  
Amperometric Method ◽  
Significant Difference ◽  
Total Antioxidant ◽  
First Time

The influence of fattening conditions on the digestive and metabolic processes in animals is actively studied to increase the production of animal food. The aim of the study was to estimate the total amount of water-soluble antioxidants in the blood serum of Duroc boars depending on the duration of fattening. Experiments were conducted on 102 animals to confirm the results of laboratory studies, as well as determination of the total antioxidant activity of pig blood serum by amperometric method. For the first time, a significant difference in antioxidant activity was found in blood samples of Duroc boars with the duration of their fattening from 81 to 101 days.

scholarly journals New Functional Ingredients Based on Microencapsulation of Aqueous Anthocyanin-Rich Extracts Derived from Black Rice (Oryza sativa L.)

Molecules ◽  
2019 ◽  
Vol 24 (18) ◽  
pp. 3389 ◽  
Author(s):  
Iuliana Aprodu ◽  
Ștefania Adelina Milea ◽  
Roxana-Mădălina Anghel ◽  
Elena Enachi ◽  
Vasilica Barbu ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Oryza Sativa ◽  
Hydrophobic Interactions ◽  
Oryza Sativa L ◽  
Milk Proteins ◽  
Added Value ◽  
Black Rice ◽  
Coating Materials ◽  
Good Thermal Stability

The aqueous anthocyanin-rich extract derived from black rice (Oryza sativa L.) was encapsulated by freeze drying using milk proteins and peptides as coating materials. The molecular modelling approach indicated that all major casein fractions and whey proteins were able to bind at least one anthocyanin molecule. The hydrophobic interactions and hydrogen bonding across the interfaces appeared to be mainly responsible for the stabilizations of the complexes formed between the coating material and bioactive compounds. Two dark purple colored powders, differentiated by the ratio of the encapsulation materials used, rich in phytochemicals were obtained, with an encapsulation efficiency of up to 99%. The powders were tested for antioxidant activity, cytocompatibility, and thermal stability. The morphological structure of the powders highlighted the presence of encapsulated anthocyanins. Both powders showed a remarkable antioxidant activity of about 46 mM Trolox/g D.W., and cytocompatibility on the L929 fibroblast culture. At certain concentrations, both powders stimulated cell proliferation. The powders showed a good thermal stability between 75 and 100 °C for 15 min. The powders were tested in a food model system and checked for stability of phytochemicals during storage. The added value of the powders was demonstrated throughout the antioxidant activity, which remained unchanged during storage.

scholarly journals Influence of the processing method on the chemical composition and antioxidant activity of raspberries

2021 ◽  
pp. 388-392
Author(s):  
И.А. Громова ◽  
М.С. Воронина ◽  
Н.В. Макарова
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Antioxidant Properties ◽  
Great Influence ◽  
Water Extract ◽  
Total Content ◽  
Waste Products ◽  
Fresh Frozen ◽  
Ethanol Extracts

Большое влияние на здоровье человека оказывают вещества, проявляющие антиоксидантный эффект, в значительной степени содержащиеся в ягодах малины и отходах сокового производства от данной ягоды, а именно свежезамороженной ягоде, свежевыжатом соке, водной вытяжке, сырых и высушенных выжимках и концентрированном соке. Целью работы стало изучение химического состава и антиоксидантной активности ягод малины и отходов сокового производства, наблюдение за изменением показателей исследуемых характеристик при механической и тепловой обработке ягод малины и выбор оптимального варианта. При проведении исследований были использованы следующие методы: определение общего содержания антоцианов; определение общего содержания фенольных соединений; определение общего содержания флавоноидов; определение антирадикальной активности; определение восстанавливающей силы; исследование антиоксидантных свойств с использованием модельной системы линолевой кислоты. Все методы по определению содержания антиоксидантов проводились на этанольных экстрактах. Установлено, что использование экстракции для ягод и отходов сокового производства позволяет более точно определить содержание исследуемых показателей. Выявлено, что наибольшая антиоксидантная активность 94,5-98,4 % отмечена в малине и водной вытяжке из нее. A great influence on human health is exerted by substances exhibiting an antioxidant effect, largely contained in raspberries and their juice waste products, specifically in fresh-frozen berries, fresh squeezed juice, water extract, raw and dried pomace and concentrated juice. The aim of the work was to study chemical composition and antioxidant activity of raspberries and their juice waste products, monitor changes in the parameters of the studied characteristics during mechanical and thermal processing of raspberries and select the optimal option. The following methods were used during the research: determination of the total content of anthocyanins; determination of the total content of phenolic compounds; determination of the total content of flavonoids; determination of antiradical activity; determination of regenerating power; study of antioxidant properties using a model system of linoleic acid. All methods for determining the content of antioxidants were carried out on ethanol extracts. It was established that using of extraction for berries and juice waste products made it possible to determine more precisely the content of the studied indicators. It was also revealed that the highest antioxidant activity of 94.5% - 98.4% was observed in raspberries and their water extract.

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