scholarly journals The Effect of Ethanolic Extract of Black and White Rice Bran (Oryza sativa L.) on Cancer Cells

2017 ◽  
Vol 21 (1) ◽  
pp. 63
Author(s):  
Rizal Maarif Rukmana ◽  
Nyoman Puniawati Soesilo ◽  
Rumiyati Rumiyati ◽  
Rarastoeti Pratiwi
Keyword(s):  
Cancer Cells ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
Probit Analysis ◽  
Rice Cultivars ◽  
Chromatography Method ◽  
White Rice ◽  
Wide Range ◽  
Black Rice Bran

Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source ofphytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activitiesof the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and‘IR­64’ (white rice) on cancer cells and to determine the compounds groups of those extracts. First step,rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphomacancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure thecytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics,flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed.The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 valuesof ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cellswere 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 onVero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. Theethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids.However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxicactivities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similarkinds of organic compounds groups but vary in the Rf values.

scholarly journals Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia

2018 ◽  
Vol 22 (1) ◽  
pp. 49 ◽  
Author(s):  
Pratiwi Apridamayanti ◽  
Rarastoeti Pratiwi ◽  
Yekti Asih Purwestri ◽  
Woro Anindito Sri Tunjung ◽  
Rumiyati Rumiyati
Keyword(s):  
Antioxidant Activity ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Rice Cultivars ◽  
Black Rice ◽  
Nutrient Contents ◽  
Macro And Micronutrients ◽  
Black Rice Bran

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

scholarly journals POTENCY OF Γ-ORYZANOL-RICH BLACK RICE BRAN (ORYZA SATIVA L. INDICA) EXTRACT FOR TYROSINASE INHIBITION

2020 ◽  
pp. 90-93
Author(s):  
AFIFAH VARDHANI ◽  
MAHDI JUFRI ◽  
ERNI PURWANINGSIH
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
High Performance ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Black Rice ◽  
Black Rice Bran ◽  
Skin Lightening

Objective: The objectives of this study were to quantify γ-oryzanol in an ethanolic extract of Oryza sativa L. Indica (black rice) bran and to evaluate its activity as a tyrosinase inhibitor. Methods: Black rice bran was extracted via maceration in 96% ethanol, and the γ-oryzanol concentration in the extract was measured through high-performance liquid chromatography. The applicability of the extract as a skin lightening agent was determined by evaluating its tyrosinase inhibition activity. Results: The dry rice bran contained 118.572 mg/g of γ-oryzanol, and the extract inhibited tyrosinase activity at an IC50 of 74.8%. Conclusion: The black rice bran extract was sufficiently potent for use in skin lightening formulations.

scholarly journals Evaluating the Efficacy of Lotion Containing Black Rice Bran (Oryza sativa L. indica) Extract as Skin Brightening Agent: A Clinical Trial

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mahdi Jufri ◽  
Afifah Vardhani ◽  
Erni Purwaningsih
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Ethanolic Extract ◽  
P Value ◽  
Rice Grain ◽  
Black Rice ◽  
Rice Bran Extract ◽  
Black Rice Bran ◽  
Skin Lightening

Background: Ultraviolet exposure is an extrinsic factor to initiate melanogenesis, the process of melanin formation in the skin. Nowadays, natural ingredients tend to be more prevalent in cosmetic formulations due to consumers’ concern about synthetic ingredients and the risks they may represent for human health. Rice bran, the outer layer of a rice grain, can be utilized as a skin-lightening agent. Objectives: This study aimed to determine the efficacy of a lotion containing black rice bran (Oryza sativa L. indica) ethanolic extract as a skin lightening agent. Methods: The black rice bran ethanolic extract was formulated into oil in water (o/w) lotion. In this study, 34 women applied the lotion at one side of the forearm and base placebo lotion as control at the other side of forearm. The results were tested with a paired t-test by GraphPad Prism 8.3.0 software. Results: There was a significant decrease in the melanin index and erythema index in the forearm with a lotion containing black rice bran extract (P-value < 0.0001). Conclusions: The lotion containing 10% black rice bran extract was effective as a skin lightener because it effectively reduced skin melanin production when applied topically.

scholarly journals Gastroprotective Activities of Ethanol Extract of Black Rice Bran (Oryza sativa L.) in Rats

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3812
Author(s):  
Peerachit Tonchaiyaphum ◽  
Warangkana Arpornchayanon ◽  
Parirat Khonsung ◽  
Natthakarn Chiranthanut ◽  
Pornsiri Pitchakarn ◽  
...  
Keyword(s):  
Gastric Ulcer ◽  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Water Immersion ◽  
Rice Variety ◽  
Ethanol Extract ◽  
Gastric Ulcers ◽  
Black Rice ◽  
Black Rice Bran

Black rice is a type of rice in the Oryza sativa L. species. There are numerous reports regarding the pharmacological actions of black rice bran, but scientific evidence on its gastroprotection is limited. This study aimed to evaluate the gastroprotective activities of black rice bran ethanol extract (BRB) from the Thai black rice variety Hom Nil (O. sativa L. indica) as well as its mechanisms of action, acute oral toxicity in rats, and phytochemical screening. Rat models of gastric ulcers induced by acidified ethanol, indomethacin, and restraint water immersion stress were used. After pretreatment with 200, 400, and 800 mg/kg of BRB in test groups, BRB at 800 mg/kg significantly inhibited the formation of gastric ulcers in all gastric ulcer models, and this inhibition seemed to be dose dependent in an indomethacin-induced gastric ulcer model. BRB could not normalize the amount of gastric wall mucus, reduce gastric volume and total acidity, or increase gastric pH. Although BRB could not increase NO levels in gastric tissue, the tissue MDA levels could be normalized with DPPH radical scavenging activity. These results confirm the gastroprotective activities of BRB with a possible mechanism of action via antioxidant activity. The major phytochemical components of BRB comprise carotenoid derivatives with the presence of phenolic compounds. These components may be responsible for the gastroprotective activities of BRB. The 2000 mg/kg dose of oral BRB showed no acute toxicity in rats and confirmed, in part, the safe uses of BRB.

scholarly journals Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells

2016 ◽  
Vol 20 (1) ◽  
pp. 69 ◽  
Author(s):  
Rarastoeti Pratiwi ◽  
Woro Anindito Sri Tunjung ◽  
R. Rumiyati ◽  
Alfi Rizqi Amalia
Keyword(s):  
Cervical Cancer ◽  
Cancer Cells ◽  
Rice Bran ◽  
Cancer Cell Line ◽  
Cervical Cancer Cell Line ◽  
Black Rice ◽  
Pigmented Rice ◽  
Cell Line Hela ◽  
Black Rice Bran ◽  
Human Cervical Cancer

Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyaninincluding cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) hasbeen evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT testwas performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain thefractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmentedrice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentageof apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract withIC50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration ofcyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively.The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fractionF3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) whilepeonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.

scholarly journals Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells

2019 ◽  
Vol 51 (1) ◽  
pp. 47-59
Author(s):  
Rarastoeti Pratiwi ◽  
◽  
Alfi Rizqi Amalia ◽  
Woro Anindito Sri Woro Anindito Sri ◽  
Rumiyati Rumiyati ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cancer Cells ◽  
Rice Bran ◽  
Breast Cancer Cells ◽  
S Phase ◽  
Phase Cell ◽  
Black Rice ◽  
Cycle Arrest ◽  
Black Rice Bran

Microwave (MW) Energy in Enzyme Deactivation: Stabilization of Rice Bran from Few Widely Consumed Indigenous Rice Cultivars (Oryza sativa L.) from Eastern Himalayan Range

2015 ◽  
Vol 11 (3) ◽  
pp. 240-245 ◽  
Author(s):  
Ashma Begum ◽  
Jahnabi Sarma ◽  
Preetismita Borah ◽  
Pinky Moni Bhuyan ◽  
Ruprekha Saikia ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Rice Bran ◽  
Oryza Sativa L ◽  
Rice Cultivars ◽  
Enzyme Deactivation

scholarly journals Phenotypic diversity of the tidal swamp rice (Oryza sativa L.) germplasm from South Kalimantan, Indonesia

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 386-394
Author(s):  
Dindin H. Mursyidin ◽  
Purnomo ◽  
Issirep Sumardi ◽  
Budi S. Daryono
Keyword(s):  
Plant Height ◽  
Quantitative Traits ◽  
Phenotypic Diversity ◽  
Oryza Sativa L ◽  
Leaf Sheath ◽  
Phenotypic Traits ◽  
Rice Cultivars ◽  
Tidal Swamp ◽  
Wide Range ◽  
The Future

Tidal swamp rice is a valuable source for developing new rice cultivars in the future because they possess some important traits for, adapted to a wide range of abiotic and biotic stresses. In this study, twenty-seven cultivars of the tidal swamp rice, comprising 26 from the South Kalimantan and one of Sumatera Island, Indonesia (an outgroup), were characterized phenotypically. Following Biodiversity International procedure, 14 qualitative and 15 quantitative traits were observed. The results show that this germplasm has a unique diversity in phenotypic traits. The secondary branch of panicle was the highest diversity of qualitative traits with an index of 0.93. Similarly, the plant height was the highest for quantitative traits (0.70). The correlation analysis confirmed that plant height, strongly correlated to culm diameter, the number of tillers and grain ratio. The PCA indicated that several independent traits, such as coleoptile anthocyanin coloration, basal leaf sheath color, and grain length, have positively contributed to the diversity, accounting 62.99%. Based on PCA analysis, the tidal swamp rice were clustered into four groups. According to UPGMA, this germplasm separated into six main groups, where Sardani (an outgroup) was very closely related to Lakatan Pacar and most distantly to Siam Perak. This information would be useful for the future rice breeding program, particularly in developing new rice cultivars in the tidal swamp areas.

scholarly journals Agarwood Branch Ethanolic Extract Affects Expression of Apoptotic Genes in MCF-7 Breast Cancer Cells

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Phirdaous Abbas ◽  
Nurhusna Samsudin ◽  
Nur Iffah Ishak ◽  
Hamzah Mohd Salleh ◽  
Saripah Salbiah Syed Abd. Azziz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Pathway Analysis ◽  
Scientific Evidence ◽  
Ethanolic Extract ◽  
Cancer Therapeutics ◽  
Wide Range ◽  
Apoptotic Pathways ◽  
Mcf 7

Breast cancer is one of the most common death causes among women worldwide. Treatment is usually associated with chemically synthesized drugs with serious side effects which shifted the attention of cancer researchers towards development of natural product alternatives. Ethnopharmaceutical evidence showed that Aquilaria spp. have been used to treat a wide range of disorders. However, scientific evidence is still lacking to support and extend the traditional applications to cancer. This study aims to investigate differential gene expression (DEG) of MCF-7 cells treated with agarwood branch ethanolic extract (ABEE) to provide insights into its cell growth-inhibiting effects. Methods: cDNA synthesis from RNA of MCF-7 cells treated with the 8 µg/ml ABEE and DMSO-treated cells (control), respectively, were subjected to RT2 Profiler Array Human Cell Death Finder™ containing 84 genes related to cell death mechanism. Pathway analysis was carried out using the online KEGG Pathway tool. Results: 48 genes that met the threshold fold regulation cut-off of 2 and p < 0.05; 41 DEGs from the list were down-regulated, and 7 were up-regulated. Pathway analysis suggested ABEE may have caused apoptosis of MCF-7 cells through extrinsic and/or intrinsic apoptotic pathways, including activation of p53 that could be the first step towards apoptotic elimination of the cancer cells upon treatment of ABEE.  Conclusion: Results obtained supported the growth inhibition effects of ABEE against MCF-7 cells that can serve as the basis for further work towards extending the use of agarwood as cancer therapeutics.

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