scholarly journals The Inhibition of Tobamovirus by Using the Extract of Banana Flower

2018 ◽  
Vol 22 (2) ◽  
pp. 181
Author(s):  
Nurviani Nurviani ◽  
Susamto Somowiyarjo ◽  
Sri Sulandari ◽  
Siti Subandiyah
Keyword(s):  
Plant Viruses ◽  
Musa Acuminata ◽  
Indicator Plant ◽  
Human Virus ◽  
Musa Balbisiana ◽  
Virus Inhibitor ◽  
Randomized Design ◽  
Necrotic Symptom ◽  
Mirabilis Jalapa ◽  
Banana Flower

Some extract of the banana plants parts have been identified to produce a compound that has efficacy as traditional medicine and human virus inhibitor. There was no previous report  on the use of the banana flower as the plants antiviral sources for plant pathogen. The objective of this study was to identify the potency  of two types of the banana flower as the Tobamovirus inhibitor substance. The antiviral was prepared from the extract of Ambon banana (Musa acuminata Colla) flower and Klutuk banana (Musa balbisiana Colla) flower, as the comparison extract of Mirabilis jalapa L. leaf which is widely known to have antiviral on various plant viruses was used. This study applied the Complete Randomized Design with three replications. Collected data includes the virus incubation period and the inhibitor power upon the local necrotic symptom on indicator plant. Results of this study showed that the extract of Ambon and Klutuk banana flower was able to inhibit the Tobamovirus with inhibition levels of 86.34% and 91.22%.IntisariBeberapa ekstrak bagian tanaman pisang diketahui memiliki kandungan senyawa yang berkhasiat sebagai obat tradisional dan zat yang dapat menghambat virus manusia. Belum pernah dilaporkan penggunaan bunga pisang sebagai sumber antiviral terhadap virus tumbuhan. Penelitian ini bertujuan untuk mengetahui potensi ekstrak bunga dua jenis pisang sebagai zat penghambat Tobamovirus. Antiviral disiapkan dari ekstrak bunga pisang Ambon (Musa acuminata Colla) dan pisang Klutuk (Musa balbisiana Colla), sebagai pembanding digunakan ekstrak daun Mirabilis jalapa L. yang sudah banyak diketahui mengandung antiviral pada berbagai virus tumbuhan. Penelitian ini dirancang menggunakan Rancangan Acak Lengkap dengan 3 ulangan. Data yang dikumpulkan meliputi masa inkubasi virus dan daya hambat gejala nekrotik lokal pada tanaman indikator. Hasil penelitian menunjukkan bahwa ekstrak bunga pisang jenis Ambon dan Klutuk mampu menghambat Tobamovirus dengan tingkat penghambatan sebesar 86,34% dan 91,22%. 

scholarly journals Genome-Wide Identification and Comparative Analysis of MYB Transcription Factor Family in Musa acuminata and Musa balbisiana

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 413
Author(s):  
Lin Tan ◽  
Usman Ijaz ◽  
Haron Salih ◽  
Zhihao Cheng ◽  
Nwe Ni Win Htet ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Expression Patterns ◽  
Musa Acuminata ◽  
Protein Domain ◽  
Myb Transcription Factor ◽  
Segmental Duplications ◽  
Musa Balbisiana ◽  
Functional Studies ◽  
Myb Genes ◽  
Gain Loss

MYB transcription factors (TFs) make up one of the most important TF families in plants. These proteins play crucial roles in processes related to development, metabolism, and stimulus-response; however, very few studies have been reported for the characterization of MYB TFs from banana. The current study identified 305 and 251 MYB genes from Musa acuminata and Musa balbisiana, respectively. Comprehensive details of MYBs are reported in terms of gene structure, protein domain, chromosomal localization, phylogeny, and expression patterns. Based on the exon–intron arrangement, these genes were classified into 12 gene models. Phylogenetic analysis of MYBs involving both species of banana, Oryza sativa, and Arabidopsis thaliana distributed these genes into 27 subfamilies. This highlighted not only the conservation, but also the gain/loss of MYBs in banana. Such genes are important candidates for future functional investigations. The MYB genes in both species exhibited a random distribution on chromosomes with variable densities. Estimation of gene duplication events revealed that segmental duplications represented the major factor behind MYB gene family expansion in banana. Expression profiles of MYB genes were also explored for their potential involvement in acetylene response or development. Collectively, the current comprehensive analysis of MYB genes in both species of banana will facilitate future functional studies.

scholarly journals Growth Induction of Cavendish Buds (Musa Acuminata L.) on Difference Concentration of IBA and BA in Vitro

2019 ◽  
Vol 8 (2) ◽  
pp. 178-187
Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani
Keyword(s):  
Musa Acuminata ◽  
Laboratory Research ◽  
Independent Effect ◽  
Randomized Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growth Induction

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.

scholarly journals Standardization of surface sterilization protocols for culture establishment in banana cv. Karpurachakkarakeli(AAB) male flower budsin vitro

2021 ◽  
Keyword(s):  
Bacterial Contamination ◽  
Male Flower ◽  
Surface Sterilization ◽  
Flower Meristem ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Short Time ◽  
Banana Flower ◽  
Banana Cultivar

Plant tissue culture is a proven technique for producing banana seeds in large quantities, uniformly and in a short time to support good quality banana seeds. The banana flower meristem can be a potential explant. The banana flower meristem offers the opportunity to regenerate plants with agronomic characteristics. This study aimed to regenerate banana flowers in vitro with different sucrose and BA (Benzyladenine) concentrations after standardized surface sterilization protocols. The study used a Completely Randomized Design (CRD), two factorial designwith surface sterilents and gelling agents. The results showed that the treatmentT15 (Sodium hypochlorite (1%) + HgCl2 (0.1%)) in G1 (0.25% gelrite) recorded the lowest fungal and bacterial contamination (0.00, 0.00) & (0.73, 0.53) respectively, in in vitro cultures of male flower buds of banana cultivar KarpuraChakkarakeli (AAB). While, the combination of BA (4 mgL-1) and sucrose (30 mgL-1) concentration had directly induced organogenesis in banana male flower explants.

scholarly journals A potent plant virus inhibitor found in Mirabilis jalapa L.

1990 ◽  
Vol 56 (4) ◽  
pp. 481-487 ◽  
Author(s):  
Susumu KUBO ◽  
Tsutomu IKEDA ◽  
Seiko IMAIZUMI ◽  
Yoichi TAKANAMI ◽  
Yoichi MIKAMI
Keyword(s):  
Plant Virus ◽  
Virus Inhibitor ◽  
Mirabilis Jalapa

scholarly journals Insights into Musa balbisiana and Musa acuminata species divergence and development of genic microsatellites by transcriptomics approach

Plant Gene ◽  
2015 ◽  
Vol 4 ◽  
pp. 78-82 ◽  
Author(s):  
Ravishankar Kundapura Venkataramana ◽  
Megha Hastantram Sampangi-Ramaiah ◽  
Rekha Ajitha ◽  
Ganesh N. Khadke ◽  
Veerraju Chellam
Keyword(s):  
Musa Acuminata ◽  
Species Divergence ◽  
Musa Balbisiana ◽  
Genic Microsatellites

Ascertaining maternal and paternal lineage within Musa by chloroplast and mitochondrial DNA RFLP analyses

Genome ◽  
2002 ◽  
Vol 45 (4) ◽  
pp. 679-692 ◽  
Author(s):  
F Carreel ◽  
D Gonzalez de Leon ◽  
P Lagoda ◽  
C Lanaud ◽  
C Jenny ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Chloroplast Dna ◽  
The Philippines ◽  
Musa Acuminata ◽  
Maternal Transmission ◽  
Musa Balbisiana ◽  
Molecular Analyses ◽  
Paternal Lineage ◽  
Wild Accessions

In banana, the maternal transmission of chloroplast DNA and paternal transmission of the mitochondrial DNA provides an exceptional opportunity for studying the maternal and paternal lineage of clones. In the present study, RFLP combined with hybridization of heterologous mitochondrial and chloroplastic probes have been used to characterize 71 wild accessions and 131 diploid and 103 triploid cultivated clones. In additon to Musa acuminata and Musa balbisiana, other species from the four Musa sections were studied to investigate their contribution to the origin of cultivated bananas. These molecular analyses enable the classification of the Musa complex to be discussed. Results ascertain relationships among and between the wild accessions and the mono- and interspecific diploid and triploid bananas, particularly for the acuminata genome. Parthenocarpic varieties are shown to be linked to M. acuminata banksii and M. acuminata errans, thus suggesting that the first center of domestication was in the Philippines – New Guinea area.Key words: Musa, RFLP, cpDNA, mtDNA, lineage.

scholarly journals PINZOTE de Musa balbisiana y Musa acuminata como Fuente de Fibras para Papel

2009 ◽  
Vol 20 (4) ◽  
Author(s):  
José Turrado ◽  
Alma R Saucedo ◽  
Rubén Sanjuán ◽  
Belkis Sulbaran
Keyword(s):  
Musa Acuminata ◽  
Musa Balbisiana
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