Pharmacological Evaluation of Antipyretic and Antioxidant Activities of 80% Methanol Root Extract and Derived Solvent Fraction of Echinops kebericho M. (Asteraceae) in Mice Model

Background. Toxicity and untoward effects are very ostensible in most standard drugs including antipyretic agents. Searching for conceivable antipyretic drugs with minimal toxicities and side effects from traditional plants is a growing concern to date. Echinops kebericho M. (Asteraceae) is one of the most prominent traditional medicinal plants, which is frequently testified for its traditionally claimed uses of treating fever and different infectious and noninfectious disorders by traditional healers in Ethiopian folk medicine. However, this plant has not been scientifically assessed for its traditionally claimed uses. This study therefore is aimed at investigating the antipyretic and antioxidant activities of 80% methanol root extract and the derived solvent fraction of Echinops kebericho M. in mouse models. Methods. Successive solvent maceration with increased polarity was used as the method of extractions, and chloroform, ethyl acetate, methanol, and water were used as solvents. After extraction, the crude extract and its derived solvent fractions were assessed for their antipyretic activities using yeast-induced pyrexia while, the antioxidant activities were measured in vitro using the diphenyl-2-picrylhydrazyl (DPPH) assay method. Both the extract and solvent fractions were evaluated at the doses of 100, 200, and 400 mg/kg for its antipyretic activities, and the antioxidant activity was evaluated at the doses of 50, 100, 200, 400, 600, 800, and 1000 mg/kg. The positive control group was treated with standard drug (ASA 100 mg/kg), while normal saline-receiving groups were assigned as negative control. Result. E. kebericho crude extract along with its derived solvent fractions showed statistically significant ( p < 0.05 , 0.01, and 0.001) temperature reduction activities. The maximum percentage of temperature reduction was observed by the highest dose (400 mg/kg) of the crude extract. The aqueous fraction also showed significantly ( p < 0.05 and 0.01) higher temperature reduction than those of ethyl acetate and chloroform fractions. The free radical scavenging activities of the crude extract were also significantly high at the maximum dose, and the aqueous fraction showed the significantly highest antioxidant activity. Conclusion. In general, the data obtained from the present study clarified that the extract possessed significant antipyretic and antioxidant activities, upholding the traditionally claimed use of the plant.

Simulation of Oldroyd-B Viscoelastic Fluid in Axisymmetric Straight Channel by Using a Hybrid Finite Element/Volume Method

In this study, incompressible viscoelastic fluid through the axisymmetric circular channel is simulated with Oldroyd-B model. The simulation is performed based on a hybrid finite volume/element method, which consists of Taylor-Galerkin finite element discretisation, and a cell vertex fluctuation-distribution finite volume method. In this context, the momentum and continuity equations are treated with a finite element method, while a finite volume approach is applied to solve the Oldroyd-B constitutive model. Analytical expressions are presented for the velocity and stress components in fully developed channel flow of Oldroyd-B fluid. For this complex fluid, we see an excellent agreement between the analytic and the numerical solutions. The study of axisymmetric circular channel problem based on a hybrid numerical method represents a great challenge. The novelty here is to study the temporal convergence-rate of the system solution that is taken to be steady state, incompressible, axisymmetric, and laminar, which did not address by researchers previously. Here, the rate of convergence for all solution components is presented, where a large level of convergence is appeared for stress compared to the other solution components. Moreover, the pressure drops and stress response across the flow are provided with respect to difference in solvent-fraction and Weissenberg number . A significant effect from the viscoelastic parameters upon the level of the stress has been detected, while for the pressure response the change is semi-modest. For the stress response the findings reveal that, with decreasing solvent-fraction , the maxima level of stress components are strongly amplifies.

Anticancer activities of phenolic compounds from Moringa oleifera leaves: in vitro and in silico mechanistic study

Background Moringa oleifera is a common vegetable in many countries since ancient times, possesses numerous phenolic compounds having a wide array of biological activities. It possesses anticancer activity that can be used to develop new drugs for treatment of various types of cancers. The current study was conducted to evaluate the composition of phenolic compounds and in vitro and in silico anticancer activities of M. oleifera leaves extracts. The leaves of M. oleifera were subjected to extraction for solvent fraction using n-hexane, chloroform, ethyl acetate, butanol, and aqueous solvents. The solvent fractions were tested for anticancer activity in vitro against Hela cancer cell line and screened for phenolic compounds through reversed-phase high-performance liquid chromatography. The molecular docking approach was employed to check binding conformations of phytochemicals against the target protein. Result The result revealed that all the solvent fractions possess in vitro anticancer activity against Hela cancer cell line. The n-hexane fraction showed a 50% reduction in Hela cancer cell viability at 416 μg mL−1 as compared to control. The extracts of solvent-fraction contained 10 phenolic compounds viz. quercetin, gallic acid, sinapic acid, vanillic acid, 4-hydroxy-3-methoxy benzoic acid, p-coumaric acid, m-coumaric acid, 4-hydroxy-3-methoxy cinnamic acid, caffeic acid, and syringic acid. Molecular docking studies revealed that the ligands bind within the active site of target protein have good binding energy values. Conclusion This study shows that M. oleifera leaves may have the potential to inhibit cancer cell growth and improving human health in addition to food ingredient innovations. Based on in vitro and in silico results, the phytochemicals from M. oleifera leaves can be used as leading drugs to treat cancer. Graphical abstract

A Tangent-Line Approach for Effective Density Used in the Ideal Mixing Rule: Part II—Evaluation of Mixing Characteristics of Oil/Gas Systems and Application Criteria

Summary Although in Part I of this study (Chen and Yang 2020) we developed a tangent-line approach for effective density that is more general, robust, and flexible than the methods proposed by Saryazdi (2012) and Saryazdi et al. (2013), its application is only limited to heavy-oil/bitumen-associated mixtures [i.e., specifically, it has only been applied to bitumen-rich liquid phase (denoted as L2)]. As indicated in Part I, the density of nitrogen (N2)/hydrocarbon mixtures cannot be accurately predicted by using the ideal mixing rule (IM) with either real density or effective density. Not only do we need to explain and evaluate the observed deviations and patterns, but also the density prediction of solvent/Fraction 1 systems [i.e., Fraction 1 of the Athabasca bitumen, which has a molecular weight (MW) of 268.8 g/mol, as reported in Azinfar et al. (2018a, 2018b, 2018c)] needs to be improved for practical use. In this study, we evaluate the mixing characteristics of different molecules in a mixture using the tangent-line approach. By evaluating and comparing performances of the IM with effective density (IM-E) and the IM with real density (IM-R), the observed patterns and deviations together with those calculated from the Westman equation indicate that the oil/gas molecules somewhat behave like solid particles in mixing. Accordingly, we further modify the effective density used in the IM to bridge the gap between the IM-E and the IM-R. The database has been extended to light-oil/gas systems such as black oils, volatile oils, gas condensates, carbon dioxide (CO2) miscible fluids, sour gases, and wet/dry gases. The IM with modified effective density (IM-ME) has also been applied to solvent/Fraction 1 systems and the C2 or C3 or n-C4-extraction L1 phase (bitumen-related mixtures) with better accuracy. Also, we develop new criteria for the uses of the IM-E, IM-ME, and IM-R that can cover the density predictions for almost all types of oil/gas systems in the petroleum industry with high accuracy. The performances of the IM are thoroughly evaluated and compared with the volume-translated (VT) Peng-Robinson equation of state (EOS) (VT PR EOS), from which the deviations provide new insights for accurately quantifying the mixture density in a more robust and reliable manner.

AKTIVITAS ANTIFOTOOKSIDASI DAN FOTOPROTEKSI FRAKSI PELARUT DARI DAUN GEDI (Abelmuschus manihot L)

ABSTRAK Penelitian ini bertujuan untuk menentukan aktivitas antifotoksidasi mengukur nilai SPF dari ekstrak metanol daun Gedi (Abelmoschus manihot L). Daun gedi diekstraksi dengan cara sokletasi. Dalam penelitian ini sampel diekstraksi dengan pelarut metanol, difraksinasi dengan pelarut petroleum eter, etil asetat, butanol dan akuades. Dari hasil fraksinasi tersebut kemudian diuji aktivitas antifotooksidasi dan fotoprotektif. Kandungan fenolik menggunakan asam galat sebagai larutan standart. Uji Aktivitas antifotooksidasi ditentukan dengan menghitung nilai diena terkojugasi yang merupakan pembentukan awal oksidasi pada lipid. Semakin rendah nila diena terkonjugasi, maka semakin baik sebagai antifotooksidasi. Untuk penentuan fotoprotektif, dilakukan dengan mneggunakan spektrofotometer UV-Vis pada panjang gelombang 290-320 nm dengan konsentrasi 100 μg/mL. Hasil penelitian ini menunjukkan bahwa fraksi etil asetat memiliki kandungan fenolik, aktivitas antioksidan, antifotooksidasi, dan fotoprotektif yang paling tinggi. Hasil yang diperoleh menunjukkan total fenol paling tinggi pada fraksi eti asetat yaitu 110.204 µg/mL, sedangkan untuk aktivitas antioksidan 57.72%. Hasil pada aktivitas antifotooksidasi menunjukkan bahwa fraksi Etil asetat menunjukkan kemampuan yang paling baik dan untuk fotoprotektik memiliki nilai SPF yang tinggi adalah etil asetat yaitu 9.03. ABSTRACT This study aimed to determine the antifotoxidation activity measuring the SPF value of the solvent fraction of the leaves of Gedi (Abelmoschus manihot L). Gedi leaves extracted by socletation. In this study the samples were extracted with methanol solvent, fractionated with petroleum ether, ethyl acetate, butanol and aquades. The results of the fractionation were then tested for antifotooxidation and photoprotective activity. Phenolic content uses gallic acid as a standard solution. The antifotoxidation activity test is determined by calculating the conjugated diene value which is the initial formation of oxidation in the lipid. The lower the conjugated diene tilapia, the better it is antifotoxidation. For photoprotective determination, a UV-Vis spectrophotometer was carried out at a wavelength of 290-320 nm with a concentration of 100 μg / mL. The results of this study indicate that the highest ethyl acetate fraction has the highest phenolic content, antioxidant activity, antifotoxidation and photoprotective. The results obtained showed the highest total phenol in the eti acetate fraction of 110,204 µg / mL, while for antioxidant activity 57.72%. The results of the anti photoxidation activity showed that the ethyl acetate fraction showed the best ability and for the photoprotectic the high SPF value was ethyl acetate namely 9.03.

IDENTIFIKASI FRAKSI AIR SENYAWA ANTIOKSIDAN MINUMAN SINOM (Curcuma domestica Val.- Tamarindus indica L.)

<p>ABSTAK<br />Sinom meminum makanan fungsional analternatif. Bahan-bahannya adalah curcumin dan daun tamarin muda, mudah didapat, relatif mahal yang dikonsumsi, mengandung senyawa antioksidan. Penelitian ini bertujuan untuk mengidentifikasi senyawa antioksidan minuman sinom terhadap perlakuan fraksi pelarut air secara in vitro. Dengan metode analisis GCMS dan TLC. Kapasitas antioksidan dalam fraksi minuman sinom air (13,91%) adalah AAEAC 1990,9 ppm dan hasil (10,94%). Fraksi air mengandung senyawa: asam lemak 9-oktadecanoid (69,43%), asam askorbat (32,7%), Ribovlafin (37,1%), ar-tumeron (1,31%), tumeron (2,85) %) dan curlon (1,09%.). Penelitian ini dengan analisis blok acak. Hasil penelitian menunjukkan fraksi air minuman sinom mengandung senyawa: asam lemak 9-oktadecanoid, asam askorbat, ribovlafin, artumeron, tumeron dan curlon. <br />Kata kunci: Minuman Sinom, fraksi pelarut air, kapasitas antioksidan, Identifikasi <br />ABSTRACT<br />Sinom beverages analternative functional food. The ingredients are curcumin and young tamarin leaves, it is easily available, relatively in expensive expected that is consumed, it contains antioxidant compounds. This study aimed to identification of sinom beverages antioxidant compounds to treatment of water solvent fraction in vitro. With GCMS and TLC analysis of method. The antioxidant capacity in beverages sinom water fraction (13,91%) is 1990,9 ppm AAEAC and yield (10,94%). The water fraction contain compounds: 9-oktadecanoid fatty acids (69,43%), ascorbic acid (32,7%), Ribovlafin (37,1%), ar-tumeron (1,31%), tumeron (2,85%) and curlon (1,09%.). This research with randomized block analysis. The results showed water fraction of sinom beverages contains compound: 9-oktadecanoid fatty acids, ascorbic acid, Ribovlafin, ar-tumeron, tumeron and curlon. <br />Keywords: Sinom beverage, water solvent fraction, antioxidant capacity, Identification</p>