scholarly journals Pengaruh variansi konsentrasi ekstrak kulit batang jambu mete terhadap sitotoksikitas sel fibroblas

2017 ◽  
Vol 2 (1) ◽  
pp. 6
Author(s):  
H. Harsini ◽  
Ahmad Febri
Keyword(s):  
Cell Viability ◽  
Optical Density ◽  
Fibroblast Cell ◽  
Stem Bark ◽  
Bark Extract ◽  
Cytotoxicity Test ◽  
Plate Reader ◽  
Stem Bark Extract ◽  
Elisa Plate ◽  
The Mean

The influence of cashew stembark extract on citotoxicity fibroblast. The aim of this study was to determine the effect of variation in the concentration of cashew stem bark extract as the base material of mouthwash of the cytotoxic effect on fibroblast cells. The material used in this study was cashew stem bark extracted by maceration method using 70% of ethanol. A total of 15 samples were grouped into 5, each of which consisted of 3 samples (ISO 10993-5). Concentrations used were 1.6%, 0.8%, 0.4%, 0.2% and 0.05%. Cytotoxicity test used the MTT method by comparing the optical density (ELISA plate reader) between treated groups with control groups. Cell viability was obtained by comparing the treated groups with control groups. Cell viability data was analyzed using one-way ANOVA and LSD. The results showed that cashew stem bark has an anticardia acid. Cytotoxicity test used the mean of fibroblast cell viability due to various cashew stem bark extracts successively from concentrations 1.6%, 0.8%, 0.4%, 0.2% and 0.05% with the mean of 15.35 ± 0.443%, 30.84% ± 1.59, 47.78 ± 8.09%, 65.74% ± 3.20, 74.95 ± 7.26%. ANOVA showed a significant influence of various cashew stem bark on cell viability (p<0,05). The results of LSD showed a significant difference between treated groups except between concentrations 0.95% and 0.2%. In conclusion, Cashew extract have anacardic acid and there was influence on various cashew stem bark extract concentrations on the cytotoxicity of fibroblast cell. The concentration of 2% was not cytotoxic.ABSTRAKTujuan penelitian ini adalah untuk mengetahui kandungan ekstrak kulit batang jambu mete dan pengaruh variasi konsentrasi terhadap sitotosisitas sel fibroblas. Penelitian ini menggunakan bahan kulit batang jambu mete (Mojolegi) yang diindentifikasi dan diekstrak menggunakan metode maserasi dengan pelarut etanol 70%. Ekstrak diuji kandungannya menggunakan metode KLT (Kromatografi  Lapis Tipis). Uji sitotoksikistas menggunakan sampel sejumlah 15 dikelompokkan menjadi 5, masing-masing kelompok 3 (ISO-10993-5). Variasi konsentrasi adalah 1,6%, 0,8%, 0,4%, 0,2% dan 0,05%. Uji sitotoksikitas menggunakan metode MTT dengan cara membandingkan optical density (ELISA plate reader) antar kelompok perlakuan dengan kelompok kontrol. Viabilitas sel didapatkan dengan membandingkan nilai optical density pada kelompok perlakuan dan kelompok kontrol. Data viabilitas sel dianalisis menggunakan ANAVA satu jalur dan LSD. Hasil penelitian menunjukkan rerata ekstrak mengandung senyawa asam anakardat dan asam galat. Uji sitotoksikistas sel fibroblas akibat variasi ekstrak kulit batang jambu mete secara berturut-turut dari konsentrasi 1,6%, 0,8%, 0,4%, 0,2% dan 0,05% dengan rerata sebesar 15,35% ± 0,443, 30,84% ± 1,59, 47,78% ± 8,09, 65,74% ± 3,20, 74,95% ± 7,26. Uji ANAVA menunjukkan adanya pengaruh variasi konsentrasi ekstrak kulit batang jambu mete bermakna terhadap viabilitas sel (p<0,05). Hasil uji LSD menunjukkan bahwa terdapat perbedaan yang bermakna (p<0,05) antar kelompok perlakuan, kecuali antara konsentrasi 0,05% dengan konsentrasi 0,2%. Kesimpulan Ekstrak kulit batang jambu mete mengandung asam anakardat dan asam galat dan terdapat pengaruh variasi konsentrasi ekstrak kulit batang jambu mete terhadap sitotoksikitas sel fibroblas. Konsentrasi 0,2% merupakan konsentrasi yang tidak toksis terhadap sel fibroblas secara in vitro. 

scholarly journals Protective Effects Of Tecomella Undulata Stem Bark Extract On Isoniazid Induced Hepatotoxicity: Based On Liver Enzymes And Histopathology In Rat Model

2019 ◽  
Vol 10 (1) ◽  
pp. 49-52
Author(s):  
Zubaida Umar Zehri ◽  
Shabih Zehra ◽  
Abdul Lateef Mahesar ◽  
M. Anwar ◽  
Syed Ijaz Hussain Zaidi ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Stem Bark ◽  
Bark Extract ◽  
Significant Difference ◽  
Function Test ◽  
Group A ◽  
Stem Bark Extract ◽  
The Mean ◽  
Group B ◽  
Group D

Objective: To evaluate the hepatoprotective effects of Tecomellaundulata stem bark extract on isoniazid induced hepatic damage based on liver enzymes and Liver function test in rat models. Study design and Setting: An experimental study conducted at Department of Pharmacology at Al-Tibri Medical College and Hospital, Isra University Karachi Campus and Dow University of Health Sciences, Karachi. Methodology: Total twenty-four rats were studied. The albino rats that were male, healthy, and weighing 200-250grams were included in this study. Rats were divided into four groups, each group having six rats and treated once daily orally for 30 days. Group A was control group and treated with normal animal diet and water; Group B was Isoniazid treated group and induced by oral administration of Isoniazid (INH) 50mg/kg. Group C was treated with Isoniazid 50mg/kg and Tecomellaundulata bark extract with low dose of 200mg/kg . Group D was treated with Isoniazid 50mg/kg and Tecomellaundulata bark extract with high dose of 400mg/kg . All the animals were weighed before commencement of the study. Liver enzymes were noted after the end of experiment. P value of <0.05 was taken as significant. Results: While comparing the mean values of AST,ALT, ALP and GGT in all four groups group; the statistical significant difference (p<0.001) was found. The mean levels in of total Bilirubin in group A was 0.69 ±0.01, group B 1.04 ±0.04, in group C was 1.15 ±0.39, and in group D was 1.04 ±0.44 with the significant difference (p=0.004). Conclusion: Tecomellaundulata has a protective effect on isoniazid induced toxicity on liver as evidenced by liver function test on rat models.

scholarly journals Anticonvulsant actions of ethanol stem bark extract of Trichilia roka (Meliaceae) in mice and chicks

2015 ◽  
Vol 4 (4) ◽  
pp. 231-234
Author(s):  
Kamaluddeen Garba ◽  
◽  
Abdullahi Yaro Hamza ◽  
Keyword(s):  
Anticonvulsant Activity ◽  
Stem Bark ◽  
Bark Extract ◽  
Maximal Electroshock ◽  
Phytochemical Screening ◽  
Bioactive Constituents ◽  
Significant Prolongation ◽  
Stem Bark Extract ◽  
The Mean ◽  
Sizeable Number

The current antiseizure drugs present with side effects, some of which can lead to discontinuation of epilepsy pharmacotherapy, and a sizeable number of other drugs being less efficacious. Medicinal plants are used for the treatment of epilepsy in Nigeria. The aim of this study is to evaluate the anticonvulsant activity of the stem bark extract of Trichilia roka in mice and chicks. Anticonvulsant screening was carried using pentylenetetrazole (PTZ), strychnine (STN) and picrotoxin (PCT) induced seizures while Maximal electroshock (MEST) test in day old chicks. Preliminary phytochemical screening of the extracts was carried out on the extract using conventional protocols. The LD50 was determined in mice. The intraperitoneal LD50 was calculated to be 118.32 mg/kg in mice. Flavonoids, tannins, alkaloids, saponins, glycosides, anthraquinones and steroids were found to be present. Significant (p≤0.05) prolongation of the mean onset of seizures was recorded with Trichilia roka (15 and 30 mg/kg) compared with normal saline treated groups in PTZ induced seizures. Trichilia roka at all the doses tested did not significantly reduce the mean recovery time of seizures induced by MEST. The extract at 7.5 and 15 mg/kg prolonged the latency of convulsion induced by STN. The extract failed to show significant prolongation of the latency of seizures induced by picrotoxin. It can then be inferred, that Trichilia roka contained bioactive constituents that are beneficial in petit mal epilepsy and justify their use in Traditional Medicine.

Sequestered uptake of chromium(VI) by Irvingia gabonensis stem bark extract anchored silica gel

2021 ◽  
Author(s):  
James F. Amaku ◽  
Segun A. Ogundare ◽  
Kovo G. Akpomie ◽  
Comfort M. Ngwu ◽  
Jeanet Conradie
Keyword(s):  
Silica Gel ◽  
Stem Bark ◽  
Bark Extract ◽  
Chromium Vi ◽  
Irvingia Gabonensis ◽  
Stem Bark Extract

scholarly journals Carbohydrate digestive enzymes are inhibited by Poincianella pluviosa stem bark extract: relevance on type 2 diabetes treatment

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Camila Gabriel Kato-Schwartz ◽  
Anacharis Babeto de Sá-Nakanishi ◽  
Ana Carolina Guidi ◽  
Geferson de Almeida Gonçalves ◽  
Fernanda Giacomini Bueno ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Digestive Enzymes ◽  
Stem Bark ◽  
Bark Extract ◽  
Diabetes Treatment ◽  
Type 2 Diabetes Treatment ◽  
Stem Bark Extract

scholarly journals Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Carica Papaya ◽  
Stem Bark ◽  
Negative Control ◽  
Diabetic Rats ◽  
Cholesterol Concentration ◽  
Bark Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

Sub-chronic Study of Aqueous Stem Bark Extract of Senna siamea in Rats

2012 ◽  
Vol 5 (6) ◽  
pp. 314-321 ◽  
Author(s):  
A. Mohammed ◽  
S.B. Mada ◽  
H.M. Yakasai
Keyword(s):  
Stem Bark ◽  
Bark Extract ◽  
Senna Siamea ◽  
Chronic Study ◽  
Stem Bark Extract
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