scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF BARK EXTRACTS OF OROXYLUM INDICUM (L) VENT

2017 ◽  
Vol 10 (8) ◽  
pp. 263
Author(s):  
Priyanka Saha ◽  
Prakash Roy Choudhury ◽  
Subrata Das ◽  
Anupam Das Talukdar ◽  
Manabendra Dutta Choudhury
Keyword(s):  
Hydrogen Peroxide ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Oroxylum Indicum ◽  
Soxhlet Apparatus ◽  
Reducing Power Assay

  Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extract of Oroxylum indicum (L) Vent, ethnomedicinally prescribed plant as hepatoprotective.Methods: Bark extracts were prepared using soxhlet apparatus. Total phenol, flavonoid, and total alkaloid were analyzed using Folin–Ciocalteu assay, aluminum chloride colorimetric assay, atropine standard, respectively. Antioxidant capacity and free radical scavenging potentialities were done by several in vitro methods, viz.; 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide scavenging activity, reducing power assay, metal chelating activity, ferric reducing ability of plasma (FRAP) assay, and thiobarbituric acid (TBA) method.Result: Total phenol, flavonoid, and alkaloid were found to be highest in methanolic extract of O. indicum (L) Vent. Methanolic extract of the bark showed very low inhibitory concentration (IC50) and effective concentration values for DPPH assay and reducing power assay compared to another extract. In hydrogen peroxide scavenging activity assay, the lowest IC50 value was recorded in methanolic extract with 153.45±0.06 μg/ml. The FRAP assay for O. indicum methanolic extract showed the highest activity at 940.09±0.07 μg/mL of ascorbic acid equivalent. The ferric reducing capacity and TBA values of the plant extracts confirmed the presence antioxidant principles in the bark of the said plant.Conclusion: These underused plants may be used for mitigating the detrimental effect of oxidative stress and reactive oxygen species-mediated disease and thus justifies its use in folklore medicines.

scholarly journals ANTIOXIDANT POTENTIAL PROFILE OF PAJANELIA LONGIFOLIA (WILLD.) K. SCHUMAN.; POTENTIAL NEW SOURCES OF NATURAL ANTIOXIDANT.

2017 ◽  
Vol 10 (6) ◽  
pp. 184
Author(s):  
Priyanka Saha ◽  
Anupam Das Talukdar ◽  
Manabendra Dutta Choudhury
Keyword(s):  
Hydrogen Peroxide ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Acetone Extract ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Soxhlet Apparatus ◽  
Reducing Power Assay

Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extracts of Pajanelia longifolia (Willd.) K. Schuman, ethnomedicinally prescribed plant as hepatoprotective. Methods: Bark extract was prepared using Soxhlet apparatus. Total phenol, flavonoid, total alkaloid were analyzed using Folin–Ciocalteu assay, aluminum chloride calorimetric assay, atropine standard, respectively. Antioxidant capacity and free radical scavenging potentialities were done by various in vitro methods, viz.; 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide scavenging activity, reducing power assay, metal chelating activity, ferric reducing antioxidant power (FRAP) assay, and thiobarbituric acid (TBA) method. Result: Total phenol, flavonoid, and alkaloid were found to be highest in acetone extract of P. longifolia (Willd.) K. Schuman. Acetone extracts of the bark of P. longifolia (Willd.) K. Schuman showed very low inhibitory concentration 50 (IC50) and effective concentration 50 values for DPPH assay and reducing power assay compared to other extracts. In hydrogen peroxide scavenging activity assay, the lowest IC50 value was recorded in acetone extract with 181.78±0.09 μg/ml in P. longifolia. The FRAP assay for P. longifolia acetone extract showed the highest activity at 967±0.69 μg/ml of ascorbic acid equivalent. The ferric reducing capacity and TBA values of the plant extracts confirmed the presence antioxidant principles in the bark of the said plant. Conclusion: These underused plants may be used for mitigating the detrimental effect of oxidative stress and reactive oxygen species-mediated disease and thus justifies its use in folklore medicines. 

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF THE METHANOLIC EXTRACT OF LINDERNIA RUELLIOIDES (COLSM.) PENNELL

2020 ◽  
pp. 141-146
Author(s):  
IMTILEMLA A ◽  
VICKY BAREH ◽  
SAMIA BEGAM BARBHUIYA ◽  
LALZIKPUII SAILO
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Fluorescence Analysis ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Reducing Power Assay ◽  
Dpph Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective of the study was collection of plant materials, Extraction of phytoconstituents using a different solvent, to carry out preliminary phytochemical screening of different extracted solvent, to perform fluorescence analysis, to estimate the proximate composition of the leaves Lindernia ruellioides (Colsm.) Pennell, and to determine the presence of in vitro anti-oxidant of the methanolic extract of the plant. Method: Preliminary phytochemical screening of the methanolic extract of Lindernia ruellioides (Colsm.) Pennell, estimation of proximate composition of the leaves, fluorescence analysis, total phenolic content, total flavonoids content, and in vitro antioxidant activity of the methanol extract (DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity). Results: The result of phytochemical screening of methanolic extract of Lindernia ruellioides (Colsm.) Pennell contents the presence of amino acid, flavonoids, tannins, steroids, and triterpenoids. The moisture content and Ash value were found to be appropriate and the in vitro antioxidant activity of the methanolic extract showed potential antioxidant activity in terms of DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity. Conclusion: The work presented here suggests that the methanolic extract of Lindernia ruellioides (Colsm.) Pennell possesses potential antioxidant activity.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

scholarly journals ANTIOXIDANT ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SIBERIAN GINSENG ROOT

2020 ◽  
pp. 38-40
Author(s):  
NAGENDAR SHETTY ◽  
V. HARIKA ◽  
SUMITRA LOKRAS
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenol ◽  
Ginseng Root ◽  
Root Extract ◽  
Frap Assay ◽  
Vitro Assay ◽  
Siberian Ginseng

Objective: This study was examined to in vitro antioxidant activity and Total Phenol and Flavonoids content analysis of methanolic root extract of Eleutherococcus senticosus (Siberian ginseng). Methods: 1,1-dephenyl-2-picryl-hydrazyl free radical scavenging and FRAP assay propose that antioxidant activity of methanol root extract because of reducing capacity of the antioxidant against oxidative effects of reactive oxygen species. Results: Scavenging activity of Siberian ginseng root RC50 value was shown 713.42±11.55 µg/ml and reducing power 0.13±0.01 mmol/g was investigated. In addition, total phenol 12.6±1.13 mg GAE/g DW and total flavonoids 9.8±0.20 mg QE/g DW were recorded. Conclusion: Although all tests were performed in vitro assay, these results recommend that Siberian ginseng root may be a good source of antioxidant ingrediant.

scholarly journals In vitro antioxidant activity of the methanolic extract of leaves of a hill tract plant Begonia roxburghii

2021 ◽  
Vol 9 (1-2) ◽  
pp. 79-89
Author(s):  
Taslima Akter ◽  
Afra Nawar ◽  
Md Nur Alam ◽  
Md Rafiquzzaman
Keyword(s):  
Ascorbic Acid ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Assay Method ◽  
Scavenging Activity ◽  
Negative Results

The study was designed to investigate the antioxidant activities of the methanolic extract of leaves of Begonia roxburghii by five different in vitro methods namely Folin-Ciocalteu, total flavonoid content, phosphomolybdenum, total reducing power determination, and DPPH scavenging activity. Phenol content (Gallic acid equivalent) was found as 180.40±0.03 mg/g of the dry sample by the Folin-Ciocalteu method. Flavonoid assay method revealed the content of flavonoids (Quercetin equivalent) as 60.43±0.27 mg/g of the dry sample. Total antioxidant capacity of the extract was observed 243.43±0.11 mg/g ( ascorbic acid equivalent) by phosphomolybdenum method. Free radical scavenging activity (measured by the DPPH test) of the methanolic extract was found 158.36±0.22 μg/mL whereas ascorbic acid, the standard, showed 19.53±0.04 μg/mL. The methanolic extract of Begonia roxburghii leaves exhibited positive and linear dose dependent reducing power activity and its activity was comparable to the standard ascorbic acid for a given dose. Phytochemical screening of the extract showed positive results for alkaloids, carbohydrates, flavonoids, glycosides, saponins, steroids, and tannins, which demonstrate definite pharmacological actions on human body. The extract showed negative results for the steroids. Jahangirnagar University J. Biol. Sci. 9(1 & 2): 79-89, 2020 (June & December)

scholarly journals COMPARATIVE EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT POTENCY OF DIFFERENT EXTRACTS OBTAINED FROM MEMECYLON SPECIES

2017 ◽  
Vol 9 (2) ◽  
pp. 187
Author(s):  
Tumkur Ramasetty Bharathi ◽  
Harishchandra Sripathy Prakash
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Methanol Extracts ◽  
Soxhlet Apparatus ◽  
Antioxidant Potency ◽  
Reducing Power Assay ◽  
Different Doses

<p><strong>Objective: </strong><em>Memecylon</em> species is being extensively used in traditional medicine for the treatment of skin disorders and it is proved to possess antidiabetic and anti-inflammatory properties. The present investigation was to study the effect of different solvent extracts of five <em>Memecylon</em> species such as <em>M. umbellatum</em>, <em>M. talbotianum</em>, <em>M. edule</em>, <em>M. malabaricum</em> and <em>M. wightii</em> on antidiabetic and antioxidant effects.</p><p><strong>Methods: </strong>Plant extracts were prepared using soxhlet apparatus using different solvents such as hexane, ethyl acetate, methanol and water and obtained extracts were subjected to antidiabetic (α-amylase and α-glucosidase inhibition assays) and antioxidant (2, 2-Diphenyl-2-Picryl Hydrazyl hydrate<strong> </strong>(DPPH), 2,2-Azino-bis (3-ethyl benzothiazoline-6-Sulfonic acid)diammonium salt<strong> </strong>(ABTS), Superoxide radical scavenging assay<strong> (</strong>SRSA) and reducing power assays) evaluated at different doses.</p><p><strong>Results: </strong>Methanol extracts of all five <em>Memecylon</em> species exhibited effective antidiabetic and antioxidant properties among them methanol extracts of <em>M. malabaricum</em> and <em>M. talbotianum</em> have highest biological activity. For α-amylase IC<sub>50</sub> value for both <em>M. malabaricum</em> and <em>M. talbotianum</em> was found to be 100 and 130 µg/reaction and IC<sub>50</sub> value for α-glucosidase was found to be 6.1 and 7.8 µg/reaction respectively. For DPPH the IC<sub>50</sub>value was found to be 190 µg/reaction, for ABTS 31-39 µg/reaction, for SRSA 950-1200 µg/reaction and for reducing power assay 420-490 µg/reaction respectively.</p><p><strong>Conclusion: </strong>The results indicate that methanol extracts of <em>M. malabaricum</em> and <em>M. talbotianum</em> possess potent <em>in vitro </em>antidiabetic and antioxidant activities compared to other <em>Memecylon</em> species.</p>

scholarly journals EXTRACTION, ISOLATION AND STRUCTURAL ELUCIDATION OF FLAVONOID FROM CHROZOPHORA PLICATA LEAVES AND EVALUATION OF ITS ANTIOXIDATIVE POTENTIALS

2017 ◽  
Vol 10 (4) ◽  
pp. 460
Author(s):  
Kadiri Sunil Kumar ◽  
Avanapu Srinivasa Rao
Keyword(s):  
Thin Layer ◽  
Superoxide Anion ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Reducing Power Assay ◽  
Isolated Compound ◽  
Layer Chromatography ◽  
Column Chromatographic

Objective: This investigation involves the extraction, isolation, and characterization of flavonoid from a Euphorbiaceae family plant Chrozophoraplicata followed by evaluation of its antioxidant principles.Methods: The dried leaves were subjected to sequential soxhlation with polar and nonpolar solvents. Methanolic extract reveals the presence of largeamount of flavonoids. Methanolic extract was subjected to isolation using column chromatographic analysis with solvents such as petroleum ether,chloroform, hexane, ethyl acetate, methanol, and water. Further, the isolated compound was subjected to thin layer chromatography technique andspectral analysis such as infrared, 1HNMR, 13CNMR, mass spectroscopy, and high performance thin layer chromatography (HPTLC) finger printingtechniques. The compound was evaluated for in vitro antioxidant studies using 2,2-diphenyl-1-picrylhydrazyl (DPPH), NO assay, reducing power assay,H2O2 scavenging assay, superoxide anion scavenging assay and β-Carotene linoleate system and in vivo antioxidative studies using carbon tetrachloride(CCl4), and acetaminophen intoxicated rats.Results: The compound was isolated in methanol:water in the ratio of 80:20 using column chromatographic technique. On the basis of phytochemical,chromatographic, and spectral analysis, the isolated compound was identified as kaempferol and finally with HPTLC finger printing technique it wasfound that the Rf value of the isolated compound was found to be 0.58 which is nearly similar to the Rf value of standard kaempferol (0.55). Hence,the isolated compound was confirmed as kaempferol and is structurally elucidated as 3,5,7-trihydroxy-2-(4-hydroxyphenyl)chromen-4-one. Thiscompound was isolated for the first time from the C. plicata leaves. The in vitro antioxidant assay of isolated flavonoid has shown a dose-dependentincrease in free radical scavenging activity using DPPH, no assay, reducing power assay, H2O2 scavenging assay, superoxide anion scavenging assay, andβ-carotene linoleate system. Further, the methanolic extract of C. plicata (MECP) leaves was subjected to single dose acute toxicity study for 14 days infemale rats on the basis of OECD guidelines 423 and the therapeutically selected doses were 200 mg/kg and 400 mg/kg. In vivo antioxidant studies inCCl4 and acetaminophen intoxicated rats indicated that the MECP leaves have significantly decreased lipid peroxidation in a dose-dependent mannerand increased the levels of catalase, superoxide dismutase, and glutathione.Conclusions: By the above results, it was concluded that the isolated compound from C. plicata leaves was confirmed as kaempferol and it possessessignificant antioxidative potentials.Keywords: Chrozophora plicata leaves, Flavonoids, Extraction, Isolation, Characterization, Methanolic extract, Antioxidant activity, Carbontetrachloride, Acetaminophen.

scholarly journals PHYTOCHEMICAL SCREENING AND EVALUATION OF LEAF PARTS OF HYPOESTES PHYLLOSTACHYA ROSEA FOR IN VITRO ANTIOXIDANT ACTIVITIES

2020 ◽  
pp. 54-58
Author(s):  
JENSON JACOB ◽  
MEENU JOHN
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Standard Procedure ◽  
Reducing Power ◽  
Phytochemical Analysis ◽  
Soxhlet Apparatus ◽  
Total Antioxidant ◽  
Phytochemical Compounds ◽  
Reducing Power Assay

Objective: The aim of this study was focussed on phytochemical analysis, in vitro antioxidant activities of different solvent extracts of Hypoestes phyllostachya leaf. Methods: The leaves were washed efficiently, shade dried and coarsely powdered. The powdered leaf material of Hypoestes phyllostachya was successively extracted with hexane, chloroform, methanol and water using Soxhlet apparatus. Preliminary phytochemical screenings for polyphenolics, proteins, phytosteroids, flavonoids, tannins, glycosides, alkaloids carbohydrates, polyphenolics, saponins were done by following standard procedure. In vitro antioxidant activities of various solvent extracts were assessed using DPPH, ABTS, Hydroxy radical, total antioxidant capacity and reducing power assay. Polyphenolics and flavonoids were also estimated. Results: The results showed that most of the phytochemical compounds such as carbohydrates, proteins, steroids, fixed oils and fatty acids, flavonoids, alkaloids, saponins, tannins, polyphenolics and glycosides present in the methanolic and aqueous extract of Hypoestes phyllostachya. The in vitro antioxidant activity of Hypoestes phyllostachya leaf clearly showed that it has better antioxidant activity, which was confirmed by different assays. Conclusion: From this work, it can be concluded that Hypoestes phyllostachya has the potential to be a good antioxidant but have only a lesser amount of flavonoids and polyphenolic contents. This was the first report on the pharmacological activity of the Hypoestes phyllostachya.

scholarly journals The STANDARDIZATION OF A TRADITIONAL POLYHERBAL FORMULATION WITH PHARMACOGNOSTIC STUDY; ITS PHYTOCHEMICAL CONTENT, ANTIOXIDANT, AND ANTIDIABETIC ACTIVITY

2019 ◽  
pp. 182-190
Author(s):  
ARUNIKA SUBBA ◽  
PALASH MANDAL ◽  
Arunika Subba
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Acetone Extract ◽  
In Vivo Studies ◽  
Antidiabetic Activity ◽  
Scavenging Activity ◽  
Phytochemical Content ◽  
Soxhlet Apparatus

Objective: Pharmacognostic study, evaluation of antioxidant and antidiabetic activity along with phytochemical contents of an ethnomedicine (AR) which is used for the treatment of arthritis and diabetes in some villages of West Sikkim. Methods: The herbal formulation was extracted in a Soxhlet apparatus successively in ten solvents from low to high polarity. The extracts were subjected to antioxidant activity, qualitative and quantitative phytochemical estimation as well as in vitro antidiabetic activity. For pharmacognostic characterization, parameters such as fluorescence activity, physicochemical values, powder microscopy, and thin-layer chromatography (TLC) were performed. Mean values with p<0.05 were considered significant in statistical analysis. Results: Pharmacognostic study revealed various plants tissues. Ash values suggested the presence of earthy materials and moisture content near to the maximum range. Variation of colors was exhibited by AR in fluorescence analysis. TLC expressed the presence of phytoconstituents and the Rf values were noted down to be used in the future for authentication of the sample. Potential antioxidant capacity was observed in AR, phenolics significantly contributing in 1,1-diphenyl-2-picrylhydrazyl scavenging activity, 2, 2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid)+ scavenging activity and reducing power. Non-polar solvent showed the presence of alkaloid and steroids. The antidiabetic activity was very high in some extracts of AR with acetone extract showing the highest enzyme inhibiting activity. IC50 of acetone extract was 0.26±0.003 mg/ml. Conclusion: Overall study established a basic reference for the formulation AR. It was considered to possess antioxidant activity, but the interesting part of the study was its antidiabetic activity which is needed to be validated with in vivo studies and toxicity assessment.

scholarly journals In vitro antioxidant and antinociceptive potentialities of methanolic extract of Litsea glutinosa

2013 ◽  
Vol 47 (4) ◽  
pp. 401-406 ◽  
Author(s):  
NN Rumzhum ◽  
MM Rahman ◽  
AA Sharukh ◽  
SA Chowdhury ◽  
MN Pervin
Keyword(s):  
Methanolic Extract ◽  
Antinociceptive Effect ◽  
Reducing Power ◽  
Antioxidant Property ◽  
Scavenging Activity ◽  
Total Antioxidant ◽  
Litsea Glutinosa

The methanolic extract of Litsea glutinosa (Lauraceae) was evaluated for in vitro antioxidant activity by determination of hydrogen peroxide scavenging activity, total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test and in vivo antinociceptive effect in acetic acid induced writhing model in swiss albino mice. The results revealed the presence of pronounced antioxidant property as compared with ascorbic acid used as standard and a dose-dependent (250 and 500 mg/kg) analgesic effect. The antioxidant and antinociceptive properties observed seem to be in good accordance with the traditional uses of Litsea glutinosa. Bangladesh J. Sci. Ind. Res. 47(4), 401-406, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.14069

Sign in / Sign up

Export Citation Format

Share Document