ANTIOXIDANT ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SIBERIAN GINSENG ROOT

Objective: This study was examined to in vitro antioxidant activity and Total Phenol and Flavonoids content analysis of methanolic root extract of Eleutherococcus senticosus (Siberian ginseng). Methods: 1,1-dephenyl-2-picryl-hydrazyl free radical scavenging and FRAP assay propose that antioxidant activity of methanol root extract because of reducing capacity of the antioxidant against oxidative effects of reactive oxygen species. Results: Scavenging activity of Siberian ginseng root RC50 value was shown 713.42±11.55 µg/ml and reducing power 0.13±0.01 mmol/g was investigated. In addition, total phenol 12.6±1.13 mg GAE/g DW and total flavonoids 9.8±0.20 mg QE/g DW were recorded. Conclusion: Although all tests were performed in vitro assay, these results recommend that Siberian ginseng root may be a good source of antioxidant ingrediant.

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Quantification of the Antioxidant Activity of Plant Extracts: Analysis of Sensitivity and Hierarchization Based on the Method Used

Antioxidants ◽

10.3390/antiox9010076 ◽

2020 ◽

Vol 9 (1) ◽

pp. 76 ◽

Cited By ~ 3

Author(s):

Natividad Chaves ◽

Antonio Santiago ◽

Juan Carlos Alías

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Plant Species ◽

Radical Scavenging ◽

Reducing Power ◽

Trolox Equivalent Antioxidant Capacity ◽

Antioxidant Compounds ◽

Frap Assay ◽

Trolox Equivalent

Plants have a large number of bioactive compounds with high antioxidant activity. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to determine which species have the highest antioxidant activity. The aim of this work was to verify whether different methods show the same sensitivity and/or capacity to discriminate the antioxidant activity of the extract of different plant species. To that end, we selected 12 species with different content of phenolic compounds. Their extracts were analyzed using the following methods: 2,2-di-phenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay, ferric reducing (FRAP) assay, Trolox equivalent antioxidant capacity (ABTS) assay, and reducing power (RP) assay. The four methods selected could quantify the antioxidant capacity of the 12 study species, although there were differences between them. The antioxidant activity values quantified through DPPH and RP were higher than the ones obtained by ABTS and FRAP, and these values varied among species. Thus, the hierarchization or categorization of these species was different depending on the method used. Another difference established between these methods was the sensitivity obtained with each of them. A cluster revealed that RP established the largest number of groups at the shortest distance from the root. Therefore, as it showed the best discrimination of differences and/or similarities between species, RP is considered in this study as the one with the highest sensitivity among the four studied methods. On the other hand, ABTS showed the lowest sensitivity. These results show the importance of selecting the proper antioxidant activity quantification method for establishing a ranking of species based on this parameter.

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Inhibition of Fe2+ Induced Lipid Peroxidation in Rats Brain by Extract of Euphorbia heterophylla in vitro

European Journal of Medicinal Plants ◽

10.9734/ejmp/2019/v27i130103 ◽

2019 ◽

pp. 1-8

Author(s):

Leye Jonathan Babatola ◽

Oluwakemisola B. Oshanimi ◽

Olanrewaju M. Oluba ◽

Lawrence Okoror ◽

Adewale Agboola Odutuga

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Methanol Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenol ◽

Dependent Manner ◽

Significant Difference

This study is sought to determine the antioxidant activity and protective ability of aqueous and methanol extractible phytochemicals from Euphorbia heterophylla leaves on lipid peroxidation induced in rat brain by pro-oxidant, in vitro. The extracts of the leaves were prepared, and the ability of the extracts is to inhibit 25 µM FeSO4 induced lipid peroxidation in isolated rats’ brain, were determined. Thereafter, total phenol content, reducing power (FRAP), Fe (II) chelating, and DPPH* free radical scavenging ability of the extracts was determined and considered as an index of antioxidant activity. The results revealed that the extracts inhibit malondialdehyde (MDA) production in the basal and pro-oxidant induced lipid peroxidised rats in a dose-dependent manner, [methanol 80.11%, aqueous 70.3%] with the methanol extract (MEE) significantly (P< 0.05) than that of aqueous extract (AEE). The methanol extract (0.74 ± 0.6 mg/g) had higher total phenol contents than the aqueous (0.57 ± 1.2 mg/g); likewise the methanol extract had higher reducing power (0.08 ± 0.2, 0.03 ± 0.1 mg/g), but had no significant difference in Fe (II) chelating ability (EC50= 0.34, 0.36) with DPPH* scavenging ability (EC50=0.075, 0.075). This antioxidant properties and the protective effect of this leaf could be harnessed in the management and prevention of degenerative diseases in association with oxidative stress.

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Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities

Food Research ◽

10.26656/fr.2017.5(6).253 ◽

2021 ◽

Vol 5 (6) ◽

pp. 187-196

Author(s):

R. Ghosh ◽

P. Barua ◽

O. Sikder ◽

S. Saha ◽

S. Mojumder ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Content ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

In Vitro Models ◽

Frap Assay ◽

Reducing Power Assay ◽

Dpph Scavenging

Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.

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The In Vitro Antioxidant Activity and Phytochemicals of Locally Consumed Plant Foods from Quezon Province, Philippines

Acta Medica Philippina ◽

10.47895/amp.v54i2.1531 ◽

2020 ◽

Vol 54 (2) ◽

Author(s):

Gracia Fe B. Yu ◽

Rachel Camille R. Cabrera ◽

Paolo Robert P. Bueno ◽

Isidro C. Sia

Keyword(s):

Nitric Oxide ◽

Antioxidant Activity ◽

Hydroxyl Radical ◽

Radical Scavenging ◽

Reducing Power ◽

Butylated Hydroxytoluene ◽

Phytochemical Analysis ◽

Frap Assay ◽

Plant Foods

Background. Quezon province has been one of the primary agricultural sources of vegetables and crops in southern Luzon due to its diverse agricultural topography. Having increased dietary awareness, consumption of antioxidant-rich foods has become relevant. Objectives. Thirteen (13) methanolic extracts of endemic Quezon plant foods were evaluated for phytochemical constituents and antioxidant potential.Methods. The plant extracts were subjected to in vitro antioxidant assays, which include DPPH [2,2-diphenyl-1picrylhydrazyl], FRAP [Fluorescence recovery after photobleaching], metal chelation, superoxide, nitric oxide, hydroxyl radical scavenging activities and MTT [ (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] reduction. Qualitative phytochemical analysis were also employed. Results. P. umbellatum, locally known as kamamba and C. longa, locally known as luyang dilaw showed high antioxidant activity using DPPH and MTT assays by 82.46±0.75% and 82.35±1.79% and 78.46±0.71% and 60.97±2.60% at 66.67 μg/mL, respectively. FRAP assay revealed a comparable reducing power with BHT (butylated hydroxytoluene) (93.61±0.56%) at 72.15 μg/mL in C. longa (92.49±1.32%), P. umbellatum (92.38±0.64%), and Z. officinale (90.33±2.06%) (luya), but found with low metal chelating activity. The highest activity against hydroxyl radical were observed in S. edule (sayote) (34.30±1.88%), P. edulis (pasyonaryo) (33.48±1.34%) and D. philippinensis (katmon) (34.71±0.85%) at 45.5 μg/mL. On the other hand, Z. officinale, C. esculenta (gabi), V. unguiculata (sitaw) fruit and D. philippinensis showed dual action, as antioxidant and as pro-oxidant, in Superoxide Scavenging and Nitric Oxide assays. Quezon province plant foods contain flavonoids, phenolic compounds, glycosides and coumarins and quinones which may explain their behavior as antioxidant.Conclusion. The study revealed that different plant foods showed different capacity to scavenge particular oxidants. However, P. umbellatum and C. longa may be considered promising sources of natural antioxidants.

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In-vitro Antioxidant and Anti-lipid Peroxidation Activity of Ethanolic Extracts of Bougainvillea shubhra, Bougainvillea peruviana and Bougainvillea bhuttiana Golden Glow: A Comparative Study

Journal of Natural Remedies ◽

10.18311/jnr/2015/475 ◽

2015 ◽

Vol 15 (1) ◽

pp. 43 ◽

Cited By ~ 1

Author(s):

Mosmi Medpilwar ◽

Darshil Maru ◽

Meenakshi Upadhyay ◽

Neha Lavania ◽

Madhavi Vernekar ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Frap Assay ◽

Ethanolic Extracts ◽

In Vitro Inhibition ◽

Reducing Power Assay

The aim of our present study was to evaluate the antioxidant and anti-lipid peroxidation activity of ethanolic extracts of Bougainvillea species viz B. shubhra, B. peruviana and B. bhuttiana golden glow. Phytochemical constituents viz. total phenolics, flavonoids and tannins were assayed using standard protocol. The antioxidant activity was studied by DPPH assay, FRAP assay, reducing power assay and in-vitro inhibition of lipid peroxidation. Of the three species, B. bhuttiana golden glow showed highest amount of phenols (6.78±0.001), flavonoids (27.7±0.012) and tannin (11.08±0.008) contents. It also showed highest antioxidant activity of 15.15± 0.008 and 8.08± 0.018 as determined by FRAP assay and reducing power assay respectively. The percent DPPH radical scavenging activity was 82.72% and in-vitro inhibition of lipid peroxidation in mitochondrial membrane was also found to be highest in B. bhuttiana golden glow.

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Curcumin-based antioxidant and glycohydrolase inhibitor compounds: Synthesis and in vitro appraisal of the dual activity against diabetes

Medicinal Chemistry ◽

10.2174/1573406416666200506083718 ◽

2020 ◽

Vol 16 ◽

Author(s):

Sajjad Esmaeili ◽

Nazanin Ghobadi ◽

Donya Nazari ◽

Alireza Pourhossein ◽

Hassan Rasouli ◽

...

Keyword(s):

Antioxidant Activity ◽

Enzyme Inhibitor ◽

Radical Scavenging Activity ◽

Curcuma Longa ◽

Radical Scavenging ◽

Reducing Power ◽

Inhibitory Effect ◽

Curcumin Derivatives ◽

Reducing Power Assay

Background: Curcumin, as the substantial constituent of the turmeric plant (Curcuma longa), plays a significant role in the prevention of various diseases, including diabetes. It possesses ideal structure features as enzyme inhibitor, including a flexible backbone, hydrophobic nature, and several available hydrogen bond (H-bond) donors and acceptors. Objective: The present study aimed at synthesizing several novel curcumin derivatives and further evaluation of these compounds for possible antioxidant and anti-diabetic properties along with inhibitory effect against two carbohydrate-hydrolyzing enzymes, α-amylase and α-glucosidase, as these enzymes are therapeutic targets for attenuation of postprandial hyperglycemia. Methods: Therefore, curcumin-based pyrido[2,3-d]pyrimidine derivatives were synthesized and identified using an instrumental technique like NMR spectroscopy and then screened for antioxidant and enzyme inhibitory potential. Total antioxidant activity, reducing power assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH• ) radical scavenging activity were done to appraisal the antioxidant potential of these compounds in vitro. Results: Compounds L6-L9 showed higher antioxidant activity while L4, L9, L12 and especially L8 exhibited the best selectivity index (lowest α-amylase/α-glucosidase inhibition ratio). Conclusion: These antioxidant inhibitors may be potential anti-diabetic drugs, not only to reduce glycemic index but also to limit the activity of the major reactive oxygen species (ROS) producing pathways.

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ASSESSMENT OF PHYTOCHEMICALS, TOTAL PHENOL, FLAVONOID CONTENT AND IN VITRO ANTIOXIDANT PROPERTY OF LARGE CARDAMOM EXTRACTS

INDIAN DRUGS ◽

10.53879/id.58.10.12627 ◽

2021 ◽

Vol 58 (10) ◽

pp. 34-41

Author(s):

Kanthlal S. K. ◽

Jipnomon Joseph ◽

Bindhu P. Paul ◽

Vijayakumar M ◽

Rema Shree A. B. ◽

...

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Reducing Power ◽

Antioxidant Property ◽

Bioactive Constituents ◽

Preliminary Assessment ◽

Reducing Power Assay ◽

Large Cardamom

Amomum subulatum, commonly known as large or black cardamom, is a commonly used spice in Indian kitchens and is traditionally used to treat various ailments. To add more knowledge about the medicinal values of the fruit, this study was conducted to evaluate the in vitro antioxidant activities of aqueous, methanol, ethanol, hydro alcohol, ethyl acetate, acetone and chloroform extracts of the fruit. Preliminary assessment was done to detect the presence of phytoconstituents using identification tests. The antioxidant activity was measured by employing methods such as diphenylpicrylhydrazyl (DPPH) radical scavenging assay, total antioxidant activity equivalent to ascorbic acid, reducing power assay and superoxide anion scavenging assay. The antioxidant activities were compared with their respective phenol and flavonoid contents. Preliminary assessment revealed that large cardamom fruit is a good source of all the bioactive constituents as well as phenol and flavonoid essential for medicinal values. The extract obtained by polar solvents showed the highest antioxidant efficacy in relation to its phenol content. Also, all the solvent-soluble fractions showed a concentration-dependent antioxidant effect. Results from our study prove that large cardamom can alleviate oxidative stress, suggesting the potential of large cardamom as a functional food

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EVALUATION OF ANTIOXIDANT AND FREE RADICAL SCAVENGING ACTIVITIES OF DIFFERENT SOLVENT EXTRACTS OF LEAVES OF PIPER UMBELLATUM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15570 ◽

2017 ◽

Vol 10 (2) ◽

pp. 274

Author(s):

Sethupandian Geetha ◽

Kokkaiah Irulandi ◽

Palanichamy Mehalingam

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Ethyl Acetate ◽

Methanol Extract ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Free Radical Scavenging ◽

Frap Assay ◽

Radical Scavenging Properties

Objective: This study was designed to determine the Total phenol, flavonoid content, antioxidant and free radical scavenging properties of different solvent extracts of Piper umbellatum. Methods: Different solvent extracts evaluated with DPPH radical scavenging activity and Reducing power activity.Results: The presence of phenol and flavonoid showed highly in the methanol extracts than ethyl acetate and acetone extracts. All the extracts have various level of antioxidant activity. Methanol solvent extract have good extraction and show significant antioxidant activity. The effect of reducing power of methanol extract revealed good antioxidant activity compare with other tested extracts.Conclusion: On the basis of the above results we concluded that methanol extract of Piper umbellatum whole plant extracts shows significant antioxidant activity than ethyl acetate and acetone extracts.Keywords: Piper umbellatum, Antioxidant, DPPH, FRAP assay, Polar, Non polar solvents

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Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3308 ◽

2020 ◽

Vol 11 (4) ◽

pp. 6262-6267

Author(s):

Krishnamoorthy Meenakumari ◽

Giridharan Bupesh ◽

Mayur Mausoom Phukan

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Biological Properties ◽

Scavenging Activity ◽

Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

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In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Biomolecules ◽

10.3390/biom10101435 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1435 ◽

Cited By ~ 1

Author(s):

Stella Maria Andrade Gomes Barreto ◽

Cesar Orlando Muñoz Cadavid ◽

Rafael Amir de Oliveira Moura ◽

Giovanna Melo Martins Silva ◽

Samara Vitória Ferreira de Araújo ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Direct Action ◽

Radical Scavenging ◽

Reducing Power ◽

New Production ◽

Industrial Residue ◽

Agave Sisalana

Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

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