scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT ACTIVITY OF THE METHANOLIC EXTRACT OF LINDERNIA RUELLIOIDES (COLSM.) PENNELL

2020 ◽  
pp. 141-146
Author(s):  
IMTILEMLA A ◽  
VICKY BAREH ◽  
SAMIA BEGAM BARBHUIYA ◽  
LALZIKPUII SAILO
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Fluorescence Analysis ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Reducing Power Assay ◽  
Dpph Scavenging ◽  
In Vitro Antioxidant Activity

Objective: The objective of the study was collection of plant materials, Extraction of phytoconstituents using a different solvent, to carry out preliminary phytochemical screening of different extracted solvent, to perform fluorescence analysis, to estimate the proximate composition of the leaves Lindernia ruellioides (Colsm.) Pennell, and to determine the presence of in vitro anti-oxidant of the methanolic extract of the plant. Method: Preliminary phytochemical screening of the methanolic extract of Lindernia ruellioides (Colsm.) Pennell, estimation of proximate composition of the leaves, fluorescence analysis, total phenolic content, total flavonoids content, and in vitro antioxidant activity of the methanol extract (DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity). Results: The result of phytochemical screening of methanolic extract of Lindernia ruellioides (Colsm.) Pennell contents the presence of amino acid, flavonoids, tannins, steroids, and triterpenoids. The moisture content and Ash value were found to be appropriate and the in vitro antioxidant activity of the methanolic extract showed potential antioxidant activity in terms of DPPH scavenging activity, reducing power assay, and nitric oxide scavenging activity. Conclusion: The work presented here suggests that the methanolic extract of Lindernia ruellioides (Colsm.) Pennell possesses potential antioxidant activity.

In vitro antioxidant and preliminary phytochemical screening of methanolic extract of Macaranga denticulata (Blume) Müll.Arg.

2018 ◽  
Vol 18 (3) ◽  
pp. 92-97
Author(s):  
R. Lalmuanawmi ◽  
Zothanpuia
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reference Standards ◽  
Butylated Hydroxytoluene ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
In Vitro Antioxidant Activity

Macaranga denticulata (Blume) Müll.Arg. (family Euphorbiaceae) is an evergreen tree and a common pioneer species in moist open and secondary forest. It is commonly known as Kharpa in Mizoram. Traditionally, the species of Macaranga are used in the treatment of swelling, cuts, sores, boils and bruises. Preliminary phytochemical screening and evaluation of in vitro antioxidant activity were carried out on the methanolic extract obtained from the bark of M. denticulata. The presence of alkaloids, tannins, flavonoids, saponins, steroids and triterpenoids was indicated by the tests conducted. The in vitro antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and hydrogen peroxide radical scavenging activity. Ascorbic acid and butylated hydroxytoluene (BHT) were used as reference standards. The methanolic extract of the plant shows a strong antioxidant activity comparable to that of the reference standards.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Methanolic Extract of Tropaeolum majus L. Seed

2017 ◽  
Vol 19 (3) ◽  
pp. 1-6
Author(s):  
Muhammad Hayat ◽  
Muhammad Wajid ◽  
Muhammad Iqbal ◽  
Anjum Khursheed ◽  
Muhammad Uzair ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Methanolic Extract ◽  
Phytochemical Screening ◽  
Tropaeolum Majus ◽  
In Vitro Antioxidant Activity

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF PHYLLODIUM PULCHELLUM L. DESV - AN THREATENED MEDICINAL PLANT

2017 ◽  
Vol 10 (10) ◽  
pp. 282
Author(s):  
Gopal Murugan Velmurugan ◽  
Subramaniam Parvathi Anand
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Threatened Medicinal Plant ◽  
In Vitro Antioxidant Activity

  Objectives: In this study, we determined the in vitro antioxidant capacity of Phyllodium pulchellum of aqueous, ethanol, and chloroform leaf extracts.Methods: In this context, the in vitro antioxidant activity was demonstrated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis(3- ethylbenzothiazolone-6-sulfonic acid) (ABTS+) radical scavenging assay, the total antioxidant activity of phosphomolybdenum assay and hydroxyl radical scavenging activity in different leaf extracts of P. pulchellum. The antioxidant activity of the extracts was compared to standard ascorbic acid.Results: All the four methods of antioxidant showed good reducing power and reducing capacity with increasing concentration again taking the ethanol leaf extract to the top position. Remarkable of antioxidant activity was observed in ethanol leaf extract on the hydrogen peroxide scavenging activity with the lowest inhibitory concentration 50 values of (155.40 μg/ml) followed by DPPH (432.90 μg/ml) and ABTS+ (524.40 μg/ml).Conclusion: These results suggest that the leaf of P. pulchellum could be a valuable source of new antioxidant properties, from the above results it seen that this plant exhibits pharmaceutical activity. 

scholarly journals Comparison of phenolic content and antioxidant activity of two common fruits of Bangladesh in solvents of varying polarities

Food Research ◽  
2021 ◽  
Vol 5 (6) ◽  
pp. 187-196
Author(s):  
R. Ghosh ◽  
P. Barua ◽  
O. Sikder ◽  
S. Saha ◽  
S. Mojumder ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
In Vitro Models ◽  
Frap Assay ◽  
Reducing Power Assay ◽  
Dpph Scavenging

Phenolic content and antioxidant activity of two common fruits of Bangladesh, namely Phyllunthus emblica and Elaeocarpus floribundus, were measured in water, methanol, ethanol, acetone and hexane extracts. Several in vitro models including phosphomolybdenum assay, DPPH free radical scavenging assay, FRAP assay and reducing power assay were used to assess the antioxidant activity of these extracts in comparison with reference antioxidants. Between the two fruits, P. emblica showed higher phenolic content and antioxidant activity in all the solvents used. In the DPPH scavenging assay, the activity of P. emblica extracts was close to reference antioxidants, ascorbic acid and BHT. Besides, considering the solvents used, extracts of both fruits had the highest phenolic and antioxidant activity in polar solvents. The correlation coefficient between total phenolics and antioxidant activities was found statistically significant. These findings suggest that P. emblica could be an excellent antioxidant resource for industries like food, pharmaceutical, and cosmetics.

scholarly journals PRELIMINARY PHYTOCHEMICAL, PHYSICOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF CLEMATIS MONTANA LEAVES

2016 ◽  
Vol 8 (4) ◽  
pp. 33 ◽  
Author(s):  
Hemlata Bhatt ◽  
Sarla Saklani ◽  
Kumud Upadhayay
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Reducing Power ◽  
Assay Method ◽  
E Coli ◽  
Antimicrobial Studies ◽  
Dpph Method ◽  
Reducing Power Assay ◽  
In Vitro Antioxidant Activity

Objective: To evaluate the phytochemical, physicochemical, antimicrobial and in-vitro antioxidant activity of Clamentis montana (Family: Rananculaceae).Methods: The extracts were evaluated for antibacterial activity against S. aureus, B. subtilis, E. coli, P. aeruginosa by cup plate method. In-vitro antioxidant activity was done by DPPH, ferrous chelating and reducing power assay method. The physicochemical parameter like a loss on drying, total ash value, foreign matter, etc. was evaluated by standard protocol.Results: The extract showed significant antibacterial activity against all test strains when compared with standard drugs amoxicillin. The extract showed significant antioxidant activity by DPPH method, reducing power assay and ferrous chelating method.Conclusion: The extract showed a dose-dependent significant antibacterial and antioxidant activity.

scholarly journals In-vitro Cytotoxicity and Antioxidant Studies of Elaeocarpus serratus

1970 ◽  
Vol 2 (2) ◽  
pp. 86-90 ◽  
Author(s):  
Most Nazma Parvin ◽  
Shammy Sarwar ◽  
Sadia Afreen Chowdhury ◽  
Hasan Mohammed Zakaria ◽  
Naz Hasan Huda
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Reducing Power ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Ic50 Value

The pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract of  stem bark of Elaeocarpus serratus were investigated for cytotoxic activity using brine shrimp lethality bioassay .The methanolic extract was also evaluated for possible antioxidant activity using nitric oxide scavenging activity and reducing power assays. In Elaeocarpus serratus, the LC50 value were found to be 14.94, 0.831 & 3.288 μg/mL in pet-ether soluble fraction (PEF), Carbon tetrachloride soluble fraction (CTSF) and Chloroform soluble fraction (CFSF) of the methanolic extract respectively. The extract showed significant antioxidant activity in nitric oxide scavenging activity and reducing power assay. In nitric oxide scavenging activity, the IC50 value of Elaeocarpus serratus extract was 89.325μg/mL while the IC50 value of ascorbic acid was 47.684μg/mL. It was found that scavenging of nitric oxide by the extract was concentration dependent.  Key words: Elaeocarpus serratus; Cytotoxicity; Antioxidant; Reducing power.DOI: 10.3329/sjps.v2i2.5829Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 86-90

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY OF BARK EXTRACTS OF OROXYLUM INDICUM (L) VENT

2017 ◽  
Vol 10 (8) ◽  
pp. 263
Author(s):  
Priyanka Saha ◽  
Prakash Roy Choudhury ◽  
Subrata Das ◽  
Anupam Das Talukdar ◽  
Manabendra Dutta Choudhury
Keyword(s):  
Hydrogen Peroxide ◽  
Methanolic Extract ◽  
Reducing Power ◽  
Total Phenol ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Oroxylum Indicum ◽  
Soxhlet Apparatus ◽  
Reducing Power Assay

  Objectives: The aim of the present work is to screen the antioxidant potentiality of the bark extract of Oroxylum indicum (L) Vent, ethnomedicinally prescribed plant as hepatoprotective.Methods: Bark extracts were prepared using soxhlet apparatus. Total phenol, flavonoid, and total alkaloid were analyzed using Folin–Ciocalteu assay, aluminum chloride colorimetric assay, atropine standard, respectively. Antioxidant capacity and free radical scavenging potentialities were done by several in vitro methods, viz.; 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide scavenging activity, reducing power assay, metal chelating activity, ferric reducing ability of plasma (FRAP) assay, and thiobarbituric acid (TBA) method.Result: Total phenol, flavonoid, and alkaloid were found to be highest in methanolic extract of O. indicum (L) Vent. Methanolic extract of the bark showed very low inhibitory concentration (IC50) and effective concentration values for DPPH assay and reducing power assay compared to another extract. In hydrogen peroxide scavenging activity assay, the lowest IC50 value was recorded in methanolic extract with 153.45±0.06 μg/ml. The FRAP assay for O. indicum methanolic extract showed the highest activity at 940.09±0.07 μg/mL of ascorbic acid equivalent. The ferric reducing capacity and TBA values of the plant extracts confirmed the presence antioxidant principles in the bark of the said plant.Conclusion: These underused plants may be used for mitigating the detrimental effect of oxidative stress and reactive oxygen species-mediated disease and thus justifies its use in folklore medicines.

scholarly journals IN VITRO ANTIOXIDANT ACTIVITY STUDIES OF AQUEOUS- AND FREEZE-DRIED FRUIT EXTRACT OF RHUS CHINENSIS AND JUICE OF CITRUS MACROPTERA, THEIR COMPARATIVE STUDIES

2020 ◽  
pp. 106-109
Author(s):  
SETINGLA SANGTAM ◽  
THANZAMI K
Keyword(s):  
Antioxidant Activity ◽  
Comparative Studies ◽  
Reducing Power ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Rhus Chinensis ◽  
Freeze Dried ◽  
Reducing Power Assay ◽  
In Vitro Antioxidant Activity

Objectives: The objective of the study is to evaluate in vitro antioxidant activity of aqueous- and freeze-dried fruits extracts of Rhus chinensis and Citrus macroptera and their comparative studies. Methods: The sumac fruit extracts were prepared by macerating in distilled water for 36 h, strained, and concentrated. Hatkora juice was used as it is for further concentration. Both the sample were dried using two methods, that is, evaporation in the water bath at 50°C and freeze-dried in a lyophilizer. The total phenolic content and total flavonoid content were determined using Folin–Ciocalteu and aluminum chloride method. The extracts were evaluated for antioxidant activity by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power assay. Results: Both sumac extract and Hatkora juice show potent antioxidant activity. When compared, the freeze-dried sample shows better activity as compared to the aqueous dried sample in both DPPH and reducing power assay. Conclusions: This study result shows high phenolic and flavonoid content which is responsible for its high antioxidant activity. This suggests that the fruits can be viewed as a potential source of natural antioxidants that can provide valuable functional ingredients useful for the prevention of diseases related to oxidative stress, dietary supplements, functional food, nutraceuticals, etc.

scholarly journals Evaluation of Antioxidant Activity of Cedrela toona Roxb. Leaf Extracts

2021 ◽  
pp. 24-31
Author(s):  
Kinjal H Shah ◽  
Piyush M Patel
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Antioxidant Potency ◽  
Reducing Power Assay ◽  
Β Carotene

Antioxidant potency of fruits of Cedrela toona Roxb. crude methanol and its fractionated extracts (hexane, acetone and water) have been investigated, employing three different established in vitro testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and β-carotene method. The methanol extract of fruits of Cedrela toona Roxb. showed the best DPPH scavenging activity with the lowest EC 50 34 μg/ml, followed by the acetone (EC 50 39 μg/ml), hexane (EC 50 48 μg/ml) and water extract (EC 50 54 μg/ml). The reducing power of Cedrela toona Roxb. fruit extracts increased steadily with increasing concentrations and varied significantly with different concentrations. The methanol and acetone extracts appeared to possess the highest significant reducing activity among the extracts. The stronger reducing power in the methanol and acetone extracts was probably due to the concentration of antioxidant compounds like flavonoids and phenolics in the extract. In the β-carotene bleaching assay, the antioxidant activities of all the fruits extracts gradually increased with increasing concentration of the extracts and varied significantly with different concentrations. The water extract showed the lowest significant antioxidant activity, while the methanol extract showed the highest significant antioxidant activity. The high antioxidant activity of methanol extract tested using β-carotene model may be correlated with the high phenolic content of the methanol extract.  In conclusion, antioxidant study suggested that fruits of Cedrela toona Roxb. are potential source of natural antioxidants. However, further investigations on in vivo antioxidant activities are highly recommended. In the present study, the antioxidant potency of Cedrela toona Roxb. crude methanol and its fractionated extracts (hexane, acetone and water) have been investigated, employing three different established in vitro testing systems, such as scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, reducing power assay and β-carotene method. There is no antioxidant study reported for Cedrela toona Roxb.Thus, antioxidant activity of Cedrela toona Roxb. was evaluated as it had not been determined previously.

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