FORMULATION AND EVALUATION OF HERBAL ORAL EMULGEL CONTAINING PSIDIUM GUAJAVA LINN. LEAVES EXTRACT (A PREVENTIVE ORAL CARE PREPARATION)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2021.v14i12.43218 ◽

2021 ◽

pp. 93-95

Author(s):

AKBAL AHMAD ◽

ABADHESH KUMAR NIRANJAN

Keyword(s):

Oral Care ◽

Research Work ◽

Ethanolic Extract ◽

Psidium Guajava ◽

Skin Permeability ◽

In Vitro Techniques ◽

Guava Leaves ◽

Permeability Studies

Objective: The present research work is based to establish the formulation and evaluation of herbal oral emulgel containing extracts of powdered Psidium guajava Linn. leaves extract. Methods: The guava leaves were collected and extracted by cold maceration process using different solvents, further ethanolic extract was used for the emulgel preparation. Emulsion and gel phase were prepared separately and mixed together in ratio of 1:1. Results: Different formulations ere developed and evaluated for the physical appearance, pH, homogeneity, spreadability, viscosity, extrudability, antibacterial activity, and in vitro skin permeability studies. In all formulated emulgel containing P. guajava Linn. Leaves, extract F2 formulation gives all satisfactory results as in discuss in this paper. Conclusion: Overall, concluded that the extract might contain anti-bacterial properties and further tests are required to prove for oral care activity using other in vivo or in vitro techniques.

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Anti-Inflammatory Effects of an Ethanolic Extract of Guava (Psidium guajava L.) Leaves In Vitro and In Vivo

Journal of Medicinal Food ◽

10.1089/jmf.2013.2936 ◽

2014 ◽

Vol 17 (6) ◽

pp. 678-685 ◽

Cited By ~ 22

Author(s):

Mi Jang ◽

Seung-Weon Jeong ◽

Somi K. Cho ◽

Kwang Seok Ahn ◽

Jong Hyun Lee ◽

...

Keyword(s):

Ethanolic Extract ◽

Psidium Guajava ◽

Anti Inflammatory

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Effect of Psidium guajava L on Biofi lm Forming Multidrug Resistant Extended Spectrum Beta Lactamase (ESBL) Producing Pseudomonas aeruginosa

Tribhuvan University Journal of Microbiology ◽

10.3126/tujm.v6i0.26574 ◽

2019 ◽

Vol 6 ◽

pp. 19-25

Author(s):

Bhagawati Khadka ◽

Moni Mahato ◽

Reshma Tuladhar ◽

Anjana Singh

Keyword(s):

Pseudomonas Aeruginosa ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Psidium Guajava ◽

Diffusion Method ◽

Clinical Samples ◽

Disk Diffusion Method ◽

Extended Spectrum Beta Lactamase ◽

Guava Leaves

Objectives: In order to investigate the relative efficacy of Psidium guajava L (guava) leave, in vitro antibacterial effect of ethanolic extract and leaf tea of guava against pathogenic Pseudomonas aeruginosa was carried out. Methods: This study was carried out at Microbiology laboratory of KIST medical college and teaching hospital, Lalitpur. Pseudomonas aeruginosa was isolated from different clinical samples. Antibiotic susceptibility of the isolates was performed by Kirby-Bauer disk diffusion method. Biofilm formation was detected by microtitre culture plate method and ESBL production by combine disk methods. Antimicrobial activity of guava leaf tea and ethanol extract of leaf were determined by agar well diffusion method. Results: Ethanol extract of fresh guava leaves exhibited higher antibacterial activity than dry and fresh leaves tea, but significantly less than the standard antibiotics. In this study, 7% of the total bacterial isolates were P. aeruginosa with 65.30% of these isolates being MDR. Similary, significant number of these MDR strains ie. 83.67% of these P. aeruginosa isolates produced biofilm. While as 6.12% of the isolated were ESBL producer. Conclusion: The leaves extract of guava have shown effective result against P. aeruginosa and could serve as good source of antibacterial agents. Guava leaves extract can be an economic alternative to antibiotics. However, active compound of this extract need to be purified and pharmacologically tested before its application.

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Morphometrical estimation of fetal heart growth at different gestational ages by In vivo and In vitro techniques

Indian Journal Of Applied Research ◽

10.15373/2249555x/may2013/155 ◽

2011 ◽

Vol 3 (5) ◽

pp. 491-494

Author(s):

Dr. Haritha Kumari Nimmagadda ◽

◽

Pooja Pant Pooja Pant ◽

Rajeev Mukhia ◽

Dr. Aruna Mukherjee

Keyword(s):

Fetal Heart ◽

In Vitro Techniques

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Current Status and Future Perspective for Research on Medicinal Plants with Anticancerous Activity and Minimum Cytotoxic Value

Current Drug Targets ◽

10.2174/1389450120666190429120314 ◽

2019 ◽

Vol 20 (12) ◽

pp. 1227-1243

Author(s):

Hina Qamar ◽

Sumbul Rehman ◽

D.K. Chauhan

Keyword(s):

Side Effects ◽

Medicinal Plants ◽

Anticancer Agents ◽

Drug Targets ◽

Psidium Guajava ◽

Current Status ◽

Future Perspective ◽

Wide Range

Cancer is the second leading cause of morbidity and mortality worldwide. Although chemotherapy and radiotherapy enhance the survival rate of cancerous patients but they have several acute toxic effects. Therefore, there is a need to search for new anticancer agents having better efficacy and lesser side effects. In this regard, herbal treatment is found to be a safe method for treating and preventing cancer. Here, an attempt has been made to screen some less explored medicinal plants like Ammania baccifera, Asclepias curassavica, Azadarichta indica, Butea monosperma, Croton tiglium, Hedera nepalensis, Jatropha curcas, Momordica charantia, Moringa oleifera, Psidium guajava, etc. having potent anticancer activity with minimum cytotoxic value (IC50 >3μM) and lesser or negligible toxicity. They are rich in active phytochemicals with a wide range of drug targets. In this study, these medicinal plants were evaluated for dose-dependent cytotoxicological studies via in vitro MTT assay and in vivo tumor models along with some more plants which are reported to have IC50 value in the range of 0.019-0.528 mg/ml. The findings indicate that these plants inhibit tumor growth by their antiproliferative, pro-apoptotic, anti-metastatic and anti-angiogenic molecular targets. They are widely used because of their easy availability, affordable price and having no or sometimes minimal side effects. This review provides a baseline for the discovery of anticancer drugs from medicinal plants having minimum cytotoxic value with minimal side effects and establishment of their analogues for the welfare of mankind.

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Nyctanthes Arbor-Tristis and its Role in the Alleviation of Arthritic Pain: A Review

Current Bioactive Compounds ◽

10.2174/1573407216666200109105439 ◽

2020 ◽

Vol 16 (8) ◽

pp. 1147-1156

Author(s):

Ruchi Singh ◽

Syed M. Hasan ◽

Amit Verma ◽

Sanjay K. Panda

Keyword(s):

Research Work ◽

Plant Distribution ◽

Pharmacological Activities ◽

Whole Plant ◽

Modern Drug ◽

Active Chemical ◽

Drug Synthesis ◽

New Compounds

Background: A plant is a reservoir of potentially useful active chemical entities which act as drugs as well as intermediates for the discovery of newer molecules and provide newer leads for modern drug synthesis. The demand for new compounds in the field of medicine and biotechnology is centuries old and with a rise in chronic diseases and resistance to existing drugs in the field of anti-infective agents, the chemicals obtained from plant sources have been an area of attraction. The whole plant has possessed multiple pharmacological activities. This is scientifically established by in-vivo and in-vitro studies. Methods: Various electronic databases such as PubMed, Science Direct, Scopus and Google were searched to collect the data of the present review. All the collected information is categorized into different sections as per the aim of the paper. Results: Fifty-six research and review papers have been studied and were included in this review article. After a detailed study, we provide a significant description of various phytochemicals present in Nyctanthes arbor-tristis Linn., which is responsible for various pharmacological activities. Twenty of studied articles gives a general introduction and ethnobotanical information about the plant, two papers contained microscopic detail of leaf and fruit. Twenty papers contained information about the phytoconstituents present in different parts of Nyctanthes arbor-tristis plant and fourteen articles reported pharmacological activities like antioxidant, anti-inflammatory, antiarthritic, antimicrobial and immunobiotic activity. Conclusion: This review explores the published research work comprising the ethnobotanical description of the subjected plant, distribution, phytochemical profile, and arthritis-related pharmacological activities.

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Hemostatic wound dressings: Predicting their effects by in vitro tests

Journal of Biomaterials Applications ◽

10.1177/0885328219831095 ◽

2019 ◽

Vol 33 (9) ◽

pp. 1285-1297 ◽

Cited By ~ 2

Author(s):

Cornelia Wiegand ◽

Martin Abel ◽

Uta-Christina Hipler ◽

Peter Elsner ◽

Michael Zieger ◽

...

Keyword(s):

Blood Clot ◽

Wound Dressings ◽

In Vitro Tests ◽

Regenerated Cellulose ◽

Oxidized Regenerated Cellulose ◽

Inflammatory Reactions ◽

In Vitro Techniques ◽

Cotton Gauze

Background Application of controlled in vitro techniques can be used as a screening tool for the development of new hemostatic agents allowing quantitative assessment of overall hemostatic potential. Materials and methods Several tests were selected to evaluate the efficacy of cotton gauze, collagen, and oxidized regenerated cellulose for enhancing blood clotting, coagulation, and platelet activation. Results Visual inspection of dressings after blood contact proved the formation of blood clots. Scanning electron microscopy demonstrated the adsorption of blood cells and plasma proteins. Significantly enhanced blood clot formation was observed for collagen together with β-thromboglobulin increase and platelet count reduction. Oxidized regenerated cellulose demonstrated slower clotting rates not yielding any thrombin generation; yet, led to significantly increased thrombin-anti-thrombin-III complex levels compared to the other dressings. As hemostyptica ought to function without triggering any adverse events, induction of hemolysis, instigation of inflammatory reactions, and initiation of the innate complement system were also tested. Here, cotton gauze provoked high PMN elastase and elevated SC5b-9 concentrations. Conclusions A range of tests for desired and undesired effects of materials need to be combined to gain some degree of predictability of the in vivo situation. Collagen-based dressings demonstrated the highest hemostyptic properties with lowest adverse reactions whereas gauze did not induce high coagulation activation but rather activated leukocytes and complement.

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Non-invasive skin sampling of tryptophan/kynurenine ratio in vitro towards a skin cancer biomarker

Scientific Reports ◽

10.1038/s41598-020-79903-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Skaidre Jankovskaja ◽

Johan Engblom ◽

Melinda Rezeli ◽

György Marko-Varga ◽

Tautgirdas Ruzgas ◽

...

Keyword(s):

Skin Cancer ◽

Relative Increase ◽

Cancer Biomarker ◽

Cancer Diagnostics ◽

Sampling Time ◽

Skin Permeability ◽

Experimental Conditions ◽

Non Invasive

AbstractThe tryptophan to kynurenine ratio (Trp/Kyn) has been proposed as a cancer biomarker. Non-invasive topical sampling of Trp/Kyn can therefore serve as a promising concept for skin cancer diagnostics. By performing in vitro pig skin permeability studies, we conclude that non-invasive topical sampling of Trp and Kyn is feasible. We explore the influence of different experimental conditions, which are relevant for the clinical in vivo setting, such as pH variations, sampling time, and microbial degradation of Trp and Kyn. The permeabilities of Trp and Kyn are overall similar. However, the permeated Trp/Kyn ratio is generally higher than unity due to endogenous Trp, which should be taken into account to obtain a non-biased Trp/Kyn ratio accurately reflecting systemic concentrations. Additionally, prolonged sampling time is associated with bacterial Trp and Kyn degradation and should be considered in a clinical setting. Finally, the experimental results are supported by the four permeation pathways model, predicting that the hydrophilic Trp and Kyn molecules mainly permeate through lipid defects (i.e., the porous pathway). However, the hydrophobic indole ring of Trp is suggested to result in a small but noticeable relative increase of Trp diffusion via pathways across the SC lipid lamellae, while the shunt pathway is proposed to slightly favor permeation of Kyn relative to Trp.

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The Use of the Chorioallantoic Membrane (CAM) of the Embryonic Chick for the Direct Assessment of Implant Toxicity

Alternatives to Laboratory Animals ◽

10.1177/026119298501300403 ◽

1985 ◽

Vol 13 (4) ◽

pp. 261-266

Author(s):

P.P. Monro ◽

D.P. Knight ◽

W.S. Pringle ◽

D.M. Fyfe ◽

J.R. Shearer

Keyword(s):

Chorioallantoic Membrane ◽

In Vitro Techniques ◽

Implant Materials ◽

Metal Foils ◽

Complex Interactions ◽

Cobalt Nickel ◽

Histological Criteria ◽

Fluid Environment

The toxicity of implant materials requires investigation prior to clinical use. We have developed a method where materials are directly applied to the chorioallantoic membrane (CAM) of 9-day-old chick embryos and toxicity is assessed using histological criteria. We evaluated the method using metal foils. The number and organisation of fibroblasts seemed to be the most useful criteria for assessing metal toxicity. Differences were greatest after 10 days of culture on the CAM. The method is sensitive enough to enable us to discriminate between the less toxic aluminium and titanium and the highly toxic cobalt, nickel and tungsten. The proposed method has advantages over in vitro techniques which provide an abnormal fluid environment and in which the more complex interactions that are possible between implant materials and tissue in vivo cannot be modelled.

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Adipose and Muscle Cell Co-Culture System: A Novel In Vitro Tool to Mimic the In Vivo Cellular Environment

Biology ◽

10.3390/biology10010006 ◽

2020 ◽

Vol 10 (1) ◽

pp. 6

Author(s):

Palaniselvam Kuppusamy ◽

Dahye Kim ◽

Ilavenil Soundharrajan ◽

Inho Hwang ◽

Ki Choon Choi

Keyword(s):

Drug Development ◽

Culture System ◽

Cell Types ◽

Culture Cell ◽

In Vitro Techniques ◽

Culture Systems ◽

Complex Interactions ◽

Cellular Environment

A co-culture system allows researchers to investigate the complex interactions between two cell types under various environments, such as those that promote differentiation and growth as well as those that mimic healthy and diseased states, in vitro. In this paper, we review the most common co-culture systems for myocytes and adipocytes. The in vitro techniques mimic the in vivo environment and are used to investigate the causal relationships between different cell lines. Here, we briefly discuss mono-culture and co-culture cell systems and their applicability to the study of communication between two or more cell types, including adipocytes and myocytes. Also, we provide details about the different types of co-culture systems and their applicability to the study of metabolic disease, drug development, and the role of secretory factors in cell signaling cascades. Therefore, this review provides details about the co-culture systems used to study the complex interactions between adipose and muscle cells in various environments, such as those that promote cell differentiation and growth and those used for drug development.

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