scholarly journals THE EFFECTS OF MIXTURES OF VARIOUS CONCENTRATIONS OF CARBOXYMETHYL CHITOSAN/AMORPHOUS CALCIUM PHOSPHATE WITH GYPSUM ON DENTIN REMINERALIZATION

2020 ◽  
pp. 13-15
Author(s):  
IKE DWI MAHARTI ◽  
ENDANG SUPRASTIWI ◽  
HASTI DWI SETIATI ◽  
ERLIYANA YAMIN ◽  
ANDI NURWALINI CAHYANI
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Carboxymethyl Chitosan ◽  
Phosphate Concentration ◽  
X Ray ◽  
Compound Material ◽  
Collagen Protein ◽  
One Way Anova ◽  
Demineralized Dentin ◽  
Scanning Electron

Objective: Carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) is a non-collagen protein analog with the ability of dentinremineralization, and gypsum is a compound material than can facilitate application of CMC/ACP. CMC/ACP concentration is claimed to increasecalcium and phosphate concentration, which indicates dentin remineralization. The aim of this study is to observe the effects of different concentrationsof CMC/ACP mixed with gypsum on dentin remineralization.Methods: Occlusal cavities were created in 18 freshly extracted teeth, and the teeth were divided into three groups. The first group consisted ofuntreated demineralized dentin, and the second and third groups were treated with mixtures of 2.5% and 5% CMC/ACP and gypsum. All groupswere checked and analyzed on the 14th day using scanning electron microscopy/energy-dispersive X-ray spectroscopy and one-way ANOVA (p<0.05).Results: Significant differences (p=0.001) of calcium and phosphate concentrations were found between Groups I, II, and III (p=0.001), whereas nodifferences were found between Groups II and III.Conclusion: Gypsum and CMC/ACP work synergistically to induce dentin remineralization. The highest dentin remineralization was shown by 5%CMC/ACP-gypsum mixture, but the level was not statistically different from that of 2.5% CMC/ACP-gypsum mixture.

scholarly journals Kemampuan Bioaktif Glass (Novamin) dan Casein Peptide Amorphous Calcium Phosphate (CPP-ACP) terhadap Demineralisasi Enamel

2019 ◽  
Vol 7 (2) ◽  
pp. 111
Author(s):  
Jeanny Kathleen H ◽  
Cecilia G.J. Lunardhi ◽  
Ari Subiyanto
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Pathological State ◽  
X Ray ◽  
Group A ◽  
Ph Cycling ◽  
Casein Phosphopeptide ◽  
Group D ◽  
Scanning Electron ◽  
Better Than

Background: Oral cavity is always associated with a dynamic atmosphere where the process of demineralization and remineralization will continue to occur. Caries is a pathological state of continuous demineralization process. Prevention of the occurrence of demineralization process can be done by enhancing remineralization, materials that can be used materials that can trigger a process of remineralization include fluoride, cpp-acp, and novamin. Purpose: The aim of this study was to evaluate and compare the potential of bioactive-Glass (Novamin) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing dentifrice on enamel demineralization. Method: A total of 24 sound human premolars were divided into 4 groups and continued into pH cycling regime to be evaluated with Scanning Electron Microscope – Energy Dispersive X-Ray. Result: Group D showed significantly higher values (P < 0.05) when compared with less demineralization lesion than Group A, B, and C. Conclusions: The ability of Novamin on demineralization lesions better than CPP-ACP.

scholarly journals Interaction of storage medium and silver diamine fluoride on demineralized dentin

2021 ◽  
Vol 49 (2) ◽  
pp. 030006052098533
Author(s):  
Jing-Yuan Peng ◽  
Michael George Botelho ◽  
Jukka Pekka Matinlinna ◽  
Hao-Bo Pan ◽  
Edwin Kukk ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Silver Chloride ◽  
Metallic Silver ◽  
Storage Medium ◽  
Silver Diamine Fluoride ◽  
X Ray ◽  
Whole Saliva ◽  
Demineralized Dentin ◽  
Scanning Electron

Objective The effects of saliva on demineralized dentin and silver diamine fluoride (SDF) were investigated in vitro. Methods Dentin samples stored in deionized water (DIW), buffer solution (BS), basal medium mucin (BMM), and unstimulated whole saliva (UWS) were demineralized for 3 days and immersed in the same storage media. SDF as a 38 mass% solution was applied to the dentin samples for 3 minutes after they had been replaced in their respective medium. Surfaces were analyzed by scanning electron microscopy, energy-dispersive X-ray analysis (EDX), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD). Results Scanning electron microscopy showed various surface deposits and coatings, including occlusion of dentinal tubules. DIW resulted in the thinnest coating, whereas BMM resulted in the thickest. EDX and XPS showed the formation of metallic silver and silver compounds in all four media, with the greatest formation in BS. XRD indicated that the main product was silver chloride except in DIW. Sulphur was found in BMM and UWS. EDX and XPS detected fluoride and XRD detected calcium fluoride and fluorohydroxyapatite in BS, BMM, and UWS. Conclusion The interaction between SDF and demineralized dentin was dependent upon the storage medium. BMM provided an outcome most similar to human saliva.

Obtaining Tetracalcium Pohosphate and Hydroxyapatite in Powder Form by Wet Method

2010 ◽  
Vol 660-661 ◽  
pp. 954-958
Author(s):  
Sara Verusca de Oliveira ◽  
Marcus Vinícius Lia Fook ◽  
Elaine Patrícia Araújo ◽  
Keila Machado Medeiros ◽  
Guilherme Portela Rabello ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Advanced Materials ◽  
Direct Reaction ◽  
Powder Form ◽  
X Ray Diffraction ◽  
X Ray ◽  
Regeneration Of Bone Tissue ◽  
Regeneration Of Bone ◽  
Hydroxyapatite Phase ◽  
Scanning Electron

The development of research in the area of advanced materials and tissue engineering has increased greatly in recent years found that bioceramics are outstanding in the replacement and regeneration of bone tissue, mainly formed by the calcium phosphate ceramics. The objective of this research is to obtain the calcium phosphate where Ca/P = 1.67 and 2.0, to observe the formation of phases after having subjected these materials to heat treatment. The calcium phosphate was produced by the wet method using a direct reaction of neutralization and characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and X-ray microanalysis (EDS). The XRD results confirm the presence of hydroxyapatite phase in the sample with Ca/P = 1.67, where as the phosphates prepared with Ca/P = 2.0 ratio show a combination of hydroxyapatite and phase β- tricalcium phosphate. The micrographs obtained are characteristic of ceramic material called calcium phosphate. EDS confirmed the presence of Ca, P and O in the material.

Tracking the interaction of drug molecules with individual mesoporous amorphous calcium phosphate/ATP nanocomposites – an X-ray spectromicroscopy study

2020 ◽  
Vol 22 (23) ◽  
pp. 13108-13117
Author(s):  
Ying-Ying Jiang ◽  
Zhi-Qiang Wang ◽  
Jia-Tang Chen ◽  
Jun Li ◽  
Ying-Jie Zhu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Adenosine Triphosphate ◽  
Amorphous Calcium Phosphate ◽  
Drug Carrier ◽  
X Ray ◽  
Drug Molecules ◽  
Important Drug

Adenosine triphosphate (ATP) biomolecules play critical roles during the formation of amorphous calcium phosphate composites (ACPC), and ACPC is an important drug carrier due to its significant advantages of biocompatibility and biodegradability.

scholarly journals REMINERALIZATION EFFECT OF POLYASPARTIC ACID IN THE POLYMER-INDUCED LIQUID PRECURSOR PROCESS ON THE INTRAFIBRILLAR DENTIN (SCANNING ELECTRON MICROSCOPE AND ENERGY DISPERSIVE X-RAY ANALYSIS)

2020 ◽  
pp. 45-48
Author(s):  
RIVANTI IRMADELA DEVINA ◽  
ADELINE CLARISSA ◽  
NILAKESUMA DJAUHARIE ◽  
KAMIZAR NAZAR ◽  
RATNA MEIDYAWATI
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Energy Dispersive ◽  
Control Group ◽  
Polyaspartic Acid ◽  
Liquid Precursor ◽  
X Ray ◽  
Demineralized Dentin ◽  
Scanning Electron

Objective: This study aims to analyze remineralization that occurred in demineralized dentin following polyaspartic acid (pAsp) in PILP immersion.Methods: Sixteen dentin block samples were immersed in demineralized solution. The samples were divided into controls (no pAsp immersion) orpAsp immersion for 3, 7, or 14 days (n’s=4). The samples were evaluated using a scanning electron microscope (SEM) to observe morphology andenergy-dispersive X-ray (EDX) to observe calcium and phosphate levels.Results: SEM revealed that pAsp immersion resulted in significantly higher dentin remineralization compared to the control group. However, therewere no significant differences among 3, 7, and 14 days of immersion. EDX revealed that remineralization occurred by the deposition of calcium andphosphate ions.Conclusion: Including pAsp in the PILP process produced rapid remineralization of demineralized dentin.

scholarly journals Comparison of the Efficacy of Different Fluoride Varnishes on Dentin Remineralization During a Critical pH Exposure Using Quantitative X-Ray Microtomography

2018 ◽  
Vol 43 (6) ◽  
pp. E308-E316
Author(s):  
A Sleibi ◽  
A Tappuni ◽  
D Mills ◽  
GR Davis ◽  
A Baysan
Keyword(s):  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Mineral Content ◽  
Test Group ◽  
Control Group ◽  
Mineral Concentration ◽  
Voxel Size ◽  
X Ray ◽  
Casein Phosphopeptide

SUMMARY Objectives: The objective of this in vitro study was to quantify the amount of mineral change in demineralized dentin at pH 5.5 after the application of dental varnishes containing fluoride with casein phosphopeptide–amorphous calcium phosphate, fluoride and bioglass, or fluoride alone. Methods and Materials: A total of 12 extracted human sound mandibular premolar root samples were coated with an acid-resistant varnish, leaving a 2 × 3 mm window at the outer root surface. These root specimens were then randomly divided into four groups and separately subjected to the demineralizing cycle at a pH of 4.8 for five days to create artificial caries-like lesions in dentin. Subsequently, each sample was imaged using quantitative x-ray microtomography (XMT) at a 15-μm voxel size. Each test group then received one of the following treatments: dental varnish containing casein phosphopeptide–amorphous calcium phosphate and fluoride (CPP-ACP, MI varnish, GC Europe), bioglass and fluoride (BGA, Experimental, Dentsply Sirona), or fluoride alone (NUPRO, Dentsply Sirona), as well as a control group, which received no treatment. These samples were kept in deionized water for 12 hours. The thin layer of varnish was then removed. All samples including the nonvarnish group were subjected to the second demineralizing cycle at pH 5.5 for five days. The final XMT imaging was then carried out following the second demineralizing cycle. XMT scan was also carried out to varnish samples at 25 μm voxel size. The change in mineral concentration in the demineralized teeth was assessed using both qualitative and quantitative image analysis. Results: There was an increase in radiopacity in the subtracted images of all varnish groups; a significant increase in mineral content, 12% for the CPP-ACP and fluoride (p≤0.05 and p≤0.001), 25% BGA (p≤0.001), and 104% fluoride alone varnish (p≤0.001). There was an increase in the size of radiolucency in the lesion area with a significant decrease in mineral content in the nonvarnish group, 10% (p≤0.05 and p≤0.001). Conclusions: There was encouraging evidence of a remineralization effect following the application of dental varnish on dentin and also an observed resistance to demineralization during the acidic challenge in all cases. However, a dental varnish containing fluoride alone appeared to have a much greater effect on dentin remineralization when compared with CPP-ACP with fluoride and bioglass with fluoride.

Preparation of Amorphous Calcium Phosphate/Triclcium Silicate Composite Powders

2009 ◽  
Vol 79-82 ◽  
pp. 1643-1646 ◽  
Author(s):  
Qing Lin ◽  
Yan Bao Li ◽  
Xiang Hui Lan ◽  
Chun Hua Lu ◽  
Zhong Zi Xu
Keyword(s):  
Calcium Phosphate ◽  
Shell Structure ◽  
Amorphous Calcium Phosphate ◽  
Chemical Precipitation ◽  
Precipitation Method ◽  
Core Shell ◽  
X Ray Diffraction ◽  
Composite Powders ◽  
X Ray ◽  
Core Shell Structure

The amorphous calcium phosphate (ACP)/tricalcium silicate (Ca3SiO5, C3S) composite powders were synthesized in this paper. The exothermal behavior of C3S determined by isothermal conduction calorimetry indicated that the ACP could be synthesis by chemical precipitation method during the induction period (stage II) of C3S. The composite powders were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The results indicated that nanosized ACP particles deposited on the surface of C3S particles to form core-shell structure at pH=10.5, and the nCa/nP of ACP could be controlled between 1.0 and 1.5. The core-shell structure is stable after sintered at 500 oC for 3 h to remove the β-cyclodextrin (β-CD). As compared with the irregular C3S particles (1~5 μm), the composite powders particles are spherical with a diameter of 40~150 μm. Therefore, to obtain the smaller size of composite powders, it is expected to avoid the aggregate of C3S particles in the aqueous solution by addition of dispersant. As compared with C3S, the composite powders may contribute better injectability, strength and biocompatibility.

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