scholarly journals OPTIMIZATION AND CHARECTERIZATION OFINTRACELLULAR ORANGE FLUORESCENT PIGMENT FROM BACILLUS ENDOPHYTICUS (AVP-9(Kf527823)

2017 ◽  
pp. 67-74
Author(s):  
Mantri Sai Ram ◽  
Nokku Pradeep Kumar ◽  
C. V. S. Bhaskar ◽  
Amrutha V. Audipudi
Keyword(s):  
Antibacterial Activity ◽  
Maximum Yield ◽  
Chemical Properties ◽  
High Yield ◽  
Agricultural Field ◽  
Orange Pigment ◽  
Fluorescent Pigment ◽  
Cultural Conditions ◽  
Physico Chemical ◽  
Bacillus Endophyticus

Objective: Our study aimed to characterize and optimize the physico-chemical properties which render the high yield of intracellular orange fluorescent pigment (IOFP) and its antibacterial activity against clinical pathogens.Methods: Intracellular orange fluorescent pigment (IOFP) extracted from Bacillus endophyticus AVP-9(Kf527823) a rhizobacteria of chilli agricultural field using different solvent systems, fractioned by TLC and analyzed for absorption maxima. Purified pigment was screened for antimicrobial activity against clinical pathogen, Optimization of cultural conditions for maximizing the yield of pigment and compared the yield in different broth media.Results: AVP-9(Kf527823) showed circular, large orange color colonies which fluoresced orange under U. V light,. Intracellular pigment affectively extracted in acetone showed a bright fluorescent orange spot on the TLC plate, and absorption maxima at 493 nm. Antibacterial activity of isolated pigment showed highest zone of inhibition against E. coli and least to Pseudomonas. Maximum yield of the pigment was achieved in modified nutrient brot (MNB)h medium containing 2% w/v nutrient broth containing1% w/v glucose,1% w/v peptone and0.5% methionine at 35 °C and pH 7.0 with 48 h of incubation period. The yield was observed maximum in MNB. Compared to lactose broth, Tryptone soya broth and peptone water.Conclusion: Bright fluorescent orange pigment of Bacterium avp-9(Kf527823) with absorption maxima at 493 indicating that the pigment shows the characters of carotenoids. Yield maximum in MNB and its potential antibacterial activity needs further investigation for pharmaceutical applications.

SYNTHESIS OF SPATIALLY-HINDERED METHYLCYCLOALKYLPHENOLS AND SOME PECULIARITIES OF THEIR AMINOMETHYLATION REACTIONS BY AMINOETHYLNONYLIMIDAZOLINE

1970 ◽  
Author(s):  
Zaur Z. Aghamaliyev ◽  
Vagif M. Abbasov ◽  
Chingiz K. Rasulov ◽  
Igrar G. Nazarov ◽  
Nigar S. Rzaeva ◽  
...  
Keyword(s):  
Maximum Yield ◽  
Chemical Properties ◽  
Mannich Bases ◽  
Point Of View ◽  
Molar Ratio ◽  
Theoretical Point ◽  
Target Product ◽  
Substituted Phenols ◽  
Catalyst Amount ◽  
Physico Chemical

The paper deals with the results of cycloalkylation of phenol with 1-methylcyclopentene, 1(3)-methylcyclohexene in the presence of aluminum phenolate catalyst and influence of various parameters on the yield of the target product. The reaction temperature was varied from 220 to 280 °C, the reaction time - from 1 to 7 h, molar ratio of phenol to cyclene – from 1:1 to 1:3 mol/mol, the catalyst amount – from 10 to 25%. Maximum yield of 2,6-di(1(3)-methylcycloalkyl)phenols is obtained under the following conditions: temperature - 260-280 °C, duration - 5-6 h, molar ratio of phenol to 1(3)-methylcycloalkene - 1:2 mol/mol and the catalyst amount is 20% based on taken phenol. Simultaneously, the yield of the target products - 2,6-di-(1(3)-methylcycloalkyl)phenols is 44.3-47.1% per taken phenol, the selectivity is 67.4 71.2% on the target product. As a result of the chromatographic studies of the products of phenol cycloalkylation with 1(3)-methylcycloalkenes in the presence of aluminum phenolate catalyst it became clear that the alkylate mainly contains 2,6-dicycloalkyl-substituted phenols (87.4-92.3%). After rectification of the alkylate at low pressure (20 mm Hg), the target products were obtained with a purity of 96.7-98.1% and their physico-chemical properties were determined. The resulting 2,6-di-(1(3)-methylcycloalkyl)phenols were aminomethylated by formaldehyde and aminoethylnonylimidazoline at the ratio of 1:2:2.From theoretical point of view, Mannich bases were obtained with yield of 65.7-71.7% by the interaction of 2,6-di-[1(3)-methylcycloalkyl]phenols with formaldehyde and aminoethylnonyl imidazoline. Physico-chemical properties of synthesized 4-hydroxy-3,5-di-(1(3)-methylcycloalkyl)benzylaminoethylnonylimidazolines were determined.

Autoclave-assisted green synthesis of silver nanoparticles using A. fumigatus mycelia extract and the evaluation of their physico-chemical properties and antibacterial activity

2018 ◽  
Vol 7 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Sarah Ghanbari ◽  
Hamideh Vaghari ◽  
Zahra Sayyar ◽  
Mohammad Adibpour ◽  
Hoda Jafarizadeh-Malmiri
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Chemical Properties ◽  
Heating Time ◽  
Gram Negative Bacteria ◽  
Synthesis Conditions ◽  
High Antibacterial Activity ◽  
Physico Chemical ◽  
Ft Ir

Abstract Silver nanoparticles (AgNPs) were synthesized using Aspergillus fumigatus (A. fumigatus) mycelia extract via the hydrothermal method. The main reducing and stabilizing groups and components of A. fumigatus extract, such as amine, hydroxyl, amid, protein, enzymes, and cell saccharide compounds, were identified by Fourier transform infrared (FT-IR). Central composition design was used to plan the experiments, and response surface methodology was applied to evaluate of the effects of independent variables, including the amount of the prepared extract (5–7 ml) and heating time (10–20 min) at 121°C and 1.5 bar), on the particle size of the synthesized AgNPs, as manifested in broad emission peak (λmax). More stable and spherical monodispersed AgNPs, with mean particle size, polydispersity index (PDI) value, and maximum ζ potential value of 23 nm, 0.270, and +35.3 mV, respectively, were obtained at the optimal synthesis conditions using 7 ml of A. fumigatus extract and heating time of 20 min. The synthesized AgNPs indicated high antibacterial activity against both Gram-positive and Gram-negative bacteria.

scholarly journals A Novel Approach towards Synthesis and Characterization of Non-Cytotoxic Gold Nanoparticles Using Taurine as Capping Agent

Nanomaterials ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 45 ◽  
Author(s):  
Akash Kumar ◽  
Nabojit Das ◽  
Neeraj Kumar Satija ◽  
Kapil Mandrah ◽  
Somendu Kumar Roy ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Critical Role ◽  
Chemical Properties ◽  
High Yield ◽  
Liver Carcinoma ◽  
Capping Agent ◽  
Gold Nanospheres ◽  
Final Size ◽  
X Ray ◽  
Physico Chemical

Metal gold nanoparticles are of great interest due to their unique physico-chemical properties and their potential to be used as nano-probes in biosensors, drug delivery, and therapeutic applications. Currently, many capping agents are used for metal gold nanoparticles, such as cetyltrimethylammonium bromide (CTAB) and tri-sodium citrate that have been reported to be toxic and hinders biological applications. To address this issue, we report, for the first time, the use of taurine as a stable non-cytotoxic capping agent for synthesizing gold nanoparticles by using an in situ wet-chemical method. This facile method resulted in monodisperse gold nanospheres with a high yield and stability. Monodisperse gold nanospheres with average diameters of 6.9 nm and 46 nm were synthesized at a high yield with controlled morphology. Temperature played a critical role in determining the size of the taurine-capped gold nanoparticles. The subtle changes in the reaction parameters had a tremendous effect on the final size of nanoparticles and their stability. The synthesized nanoparticles were characterized by using optical spectroscopy, a ZetaSizer, a NanoSight, Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), X-ray Photon Spectroscopy (XPS) and Electron Microscopy to understand their physico-chemical properties. Taurine was explored as a capping and stabilizing agent for gold nanospheres, which were evaluated for their toxicity responses towards human liver carcinoma cells (HepG2) via MTT assay.

scholarly journals PHOTO EFFECT AND BIO-AUTOGRAPHIC STUDIES OF INTRACELLULAR ORANGE FLUORESCENT PIGMENT PRODUCED BY Bacillus endophyticus

2021 ◽  
Vol 4 (3) ◽  
pp. e202
Author(s):  
Sairam Mantri ◽  
Chandini Sulthana Syed ◽  
Naga Rathna Supriya G ◽  
Amrutha Valli Audupudi
Keyword(s):  
Antibacterial Activity ◽  
Shigella Flexneri ◽  
Salmonella Typhi ◽  
Pigment Production ◽  
Live Cells ◽  
Human Pathogens ◽  
Fluorescent Pigment ◽  
Bacillus Endophyticus ◽  
Back Ground ◽  
Bacterium Bacillus

Present study has focused on the effect of chemical (solvents) and physical (photo) conditions on pigment production and its bioactivity of intracellular orange fluorescent pigment (IOFP) extracted from soil bacterium Bacillus endophyticus. Standardization of pigment and its colour stability was confirmed by using different solvents (70% & 100% ethanol, hexane, heptane, ethyl acetate, acetone, petroleum ether, chloroform, methanol and distilled water), photo conditions (Dark, U.V light and White light) on pigment production and its bio-activeness by antibacterial activity using agar cup plate method against gram-positive (Staphylococcus aureus, streptococcus pyogenes and Listeria monocytogenes) and gram-negative (Salmonella typhi, Vibrio cholera, Shigella Flexneri and E.coli) human pathogens and purification of pigment by TLC coupled with bio-autographic studies. Acetone is proved to be the best solvent for extraction and the pigment was stable in all solvents without changing its colour except heptane. When compared to control (dark incubation) antibacterial activity of IOFP produced in U.V and W. Light was effective against all tested pathogens with slight differences in their antibacterial activity. TLC bio-autographic studies reveal that the separated pure band shows clear zone of inhibition under red back ground of live cells stating that, the compound is active against human bacterial pathogens. Hence this study concludes that, the production and biological activity of the IOFP was independent of light incubation, and TLC guided bio-autographic approach offers a rapid detection technique that avoids the testing of purified fraction once again.

scholarly journals Gamma radiation as a green method to enhance the dielectric behaviour, magnetization, antibacterial activity and dye removal capacity of Co–Fe LDH nanosheets

RSC Advances ◽  
2019 ◽  
Vol 9 (56) ◽  
pp. 32544-32561 ◽  
Author(s):  
Rafat M. Amin ◽  
Mohamed Taha ◽  
S. A. Abdel Moaty ◽  
Fatma I. Abo El-Ela ◽  
Hossam F. Nassar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Gamma Radiation ◽  
Dye Removal ◽  
Chemical Properties ◽  
Dielectric Behaviour ◽  
Green Method ◽  
Removal Capacity ◽  
Physico Chemical

Nowadays, improving the physico-chemical properties of nanomaterials to enhance their performance towards various applications is urgent.

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