scholarly journals ANTIOXIDANT, ANTIMICROBIAL ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SAMBUCUS NIGRA (ELDERBERRY)

2020 ◽  
pp. 35-37
Author(s):  
N. SRINIVAS GOUD ◽  
GOBIND PRASAD
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Pseudomonas Putida ◽  
Colorimetric Method ◽  
Total Phenol ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Sambucus Nigra ◽  
Fruit Extract

Objective: The goal of this study was paying attention on the chemical characterization of phytochemical compounds and their antioxidant activity of Sambucus nigra L. Methods: Phytochemical analysis was performed by Sambucus nigra L fruit extract. Total Phenol and Flavonoids content of elderberry fruit extract also determined using Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method. Antibacterial activity was performed by disk diffusion method and Antioxidant capacity was investigated by DPPH assay, butylhydroxytoluene used as a standard. Results: The richest anthocyanin in elderberry fruits was cyanidin-3-O-sambubioside. The antioxidant capacity obtained for elderberry extract proved that elderberry shown highest antioxidant activity, being the richest anthocyanins. The antioxidant capacity of elderberry fruit methanolic extract was recorded 62.56±1.12 percentages of scavenging activity. We also investigated antibacterial activity against four species Escherichia coli, Pseudomonas putida, Bacillus cereus, and Staphylococcus aureus. There E. coli was recorded 12.0 mm and Pseudomonas putida was recorded 0.34 mm zone of inhibition. Conclusion: The conclusion of our study is that Sambucus nisgra fruit extract has very high antioxidant activity which makes it recommendable for food industry and dietary supplement.

In vitro Antioxidant, Antibacterial Activities and HPLC-TOF/MS Analysis of Anvillea radiata (Asteraceae) Extracts

2019 ◽  
Vol 15 (4) ◽  
pp. 376-383
Author(s):  
Abderrahim Benslama ◽  
Abdenassar Harrar ◽  
Fatih Gül ◽  
Ibrahim Demirtaş
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Antioxidant Capacity ◽  
Fumaric Acid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Ms Analysis ◽  
Tof Ms

Background:Algeria has a particular geographical position with a wide band of very varied vegetation including the aromatic and medicinal plants. These plants are able to produce much diversified natural compounds, which can be very useful health care and treatment of diseases.Objective:This study was carried out to evaluate the antioxidant capacity and antibacterial activity of Anvillea radiata (Asteraceae) extracts, one of the medicinal plants used as food and in folk medicine in Algeria.Methods:The extraction was carried on the aerial part by methanol and water to give Met.E and Aq.E. The ABTS and DPPH free radicals-scavenging tests are used to evaluate the antioxidant activity of extracts. Moreover, the antibacterial activity was evaluated using disc diffusion method. Finally, the extracts were subject to the phytochemical analysis using the HPLC-TOF/MS equipment.Results:The Met.E showed the highest amount of total phenolic and flavonoids content (266.12 µg EAG/mg E and 50.83 µg EQ/mg E, respectively). The results of the antioxidant activity revealed that the Met.E showed a good scavenging activity against ABTS and DPPH free radical with EC50=0.067 mg/ml and EC50=0.33 mg/ml, respectively. Moreover, the A. radiata extracts present a highest antibacterial activity against M. luteus NRRL B-4375, B. subtilis NRRL NRS-744 and S. aureus ATCC 25923, with inhibition zone ranging from 18 to 23 mm to Met.E and 12-18 mm to the Aq.E. In addition, the Met.E of A. radiata showed a better MIC against S. aureus, M. luteus and B. subtilis (MIC= 6.25, 25 and 25 µg/ml, respectively). The HPLC-TOF/MS analysis of Met.E reveals the presence of two major compounds, rutin and fumaric acid, when Aq.E contains the syringic acid as a major compound.Conclusion:The data of our study reported that the Met.E extract of A. radiata had a considerable antioxidant capacity and antibacterial effect, which may be due to their richness by phenolic compounds such as rutin and fumaric acid.

scholarly journals Antibacterial Potential of some Plant Extracts against Escherichia coli, along with its Phytochemical Analysis, Total Phenolic Content, and Antioxidant Properties

2019 ◽  
Vol 5 (02) ◽  
pp. 96-102
Author(s):  
Pooja Singh ◽  
Upma Srivastava
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Butylated Hydroxytoluene ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis

In vitro antibacterial activity of 21 plant methanolic extracts were investigated by disc diffusion method against gram-negative bacteria Escherichia coli. Amongst the extracts tested, the Datura stramonium L. and Ocimum basilicum L. showed significant antibacterial activity against the bacterial pathogen. D. stramonium showed the highest antibacterial activity followed by O. basilicum extract. Phytochemical analysis of extracts showed the presence of alkaloids, tannins, saponins, flavonoids, and phenols. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ABTS analysis of potent extract D. stramonium showed appreciable antioxidant activity. For DPPH and ABTS assay the highest antioxidant activity was observed at 60 mg ml-1 concentration with a percent inhibition of 71.66 and 77.19 with IC50 value 9.71 and 22.16 μg ml-1 respectively. Moreover, the radical scavenging activity of the extract was lower than that observed for the synthetic antioxidants butylated hydroxyanisole (BHA )and butylated hydroxytoluene (BHT). The total phenolic content of D. stramonium extract was found to be 0.592 mg GAE/gm which was assayed using Folin ciocalteau reagent. The results provide evidence that the extract of D. stramonium and O. basilicum can be further recommended in the treatment of the infections caused by E. coli; further D. stramonium is also a potential source of natural antioxidants.

scholarly journals PHYSICOCHEMICAL, PHYTOCHEMICALS AND ANTIOXIDANT EVALUATION OF GUAZUMA ULMIFOLIA FRUIT

2018 ◽  
Vol 10 (9) ◽  
pp. 87
Author(s):  
Bhavani Duraiswamy ◽  
Malairajan Singanan ◽  
Vanitha Varadarajan
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Colorimetric Method ◽  
Water Soluble ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Guazuma Ulmifolia

Objective: The present study was designed to evaluate the physicochemical, qualitative, quantitative analysis and antioxidant activity of ethanol fruit extract of Guazuma ulmifolia. Studies were carried out to detect the bioactive component.Methods: The physiochemical properties such as loss on drying, extractive value for ethyl acetate, acetone, ethanol, total ash, acid insoluble ash, water-soluble ash and PH values; the phytochemical such as tannins, phenol, terpenoids, flavonoids, saponin, quinones, cardiac glycosides, coumarin, steroids and acids were found using standard methods. The total phenol and flavonoid content were estimated by Folinciocalteu and Aluminium chloride by colorimetric method. In vitro antioxidants, properties were evaluated by DPPH and FRAP assay.Results: Phytochemical analysis reveals the availability of secondary metabolites like tannins, phenol, terpenoids, flavonoids, coumarin, steroids, and cardiac glycosides in the different extract. The quantitative analysis for ethanolic fruit extract was done for total phenolic content (TPC), total flavonoids content (TFC). An ethanolic extract found to have high TPC [27.2797±0.1756] than TFC. Antioxidant activity was evaluated by DPPH assay (2, 2-diphenyl-1-picrylhydrazyl-hydrate) and FRAP (ferric Reducing Ability of Plasma).Conclusion: On the basis of this study, it was found that the Guazuma ulmifolia fruits have potential to act as a source of useful drugs due to the presence of various active phytochemical constituent, which will be helpful in preventing various diseases caused by the oxidative stress.

scholarly journals Assessment of antioxidant and antibacterial activity of Phoenix dactylifera L. seed extracts: Perspective for the development of new foods

2020 ◽  
Vol 4 (08) ◽  
pp. 298-308
Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Phoenix Dactylifera ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Dpph Method

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with the colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.

Development of Gel Dossage Form From Mulberry Fruit Extract as A Facial Treatment

2018 ◽  
Vol 9 (01) ◽  
Author(s):  
Dhanesh Arif ◽  
Diah Lia Aulifa ◽  
Driyanti Rahayu ◽  
Arni Praditasari
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Vitamin C ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Microdilution Method ◽  
Mulberry Fruit ◽  
Ic50 Value ◽  
Dpph Method

Skin care is very important in cosmetics, especially facial treatments. Black mulberry is rich in phenols and is therefor usable in the treatment of acne. It also contains anthocyanin, a well-known antioxidant and a potential source of sun protection. This research aimed to develop a gel dossage from black mulberries (Morus nigra) extracts that would function as an antibacterial, antioxidant and sun blocking facial treatment. This research started with black mulberry fruit extracted through the maceration method by using ethanol (96%). Then, the antibacterial activity of the extract was determined by the disc-diffusion method, while the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined by the microdilution method. Antioxidant activity of the extract was determined using the 1,1- diphenyl-2-picrilhidrazil (DPPH) method with vitamin C as a comparison. Furthermore, the extracts were formulated into gel formulas with variations of HPMC, Na-CMC, carbopol 934 and extract concentrations. The products were then physically evaluated for organoleptics, homogeneity, pH, viscosity, and dispersion as well as undergoing a hedonic test, an irritation test, an antioxidant activity test and determining the SPF value of the preparation. The results showed that the black mulberry fruit extract has antibacterial activity with MIC value of 0,025 g/mL against S. epidermidis and P. acnes, while MBC values were 0,025 g/mL and 0,05 g/mL, respectively. The black mulberry extract had antioxidant activity with IC50 value, i.e 146,73 μg/mL vitamin c i.e. 3,17 μg/mL. Formulation with best physical evaluation results showed ina formula containing a carbopol gel base of 934 0,015 g/mL with an extract cconcentration of 0,075 g/mL. This formula resulted in antioxidant activity with an IC50 value, i.e 1004,6 μg/mL, antibacterial activity with inhibition zone 6.83 ± 1.4 mm against and 6.76 ± 0.9 mm against S. epidermidis and P. acnes respectively and an SPF value of 1.9.

scholarly journals Assessment of antioxidant and antibacterial activity of Phoenix dactylifera L. seed extracts: Perspective for the development of new foods

2020 ◽  
Vol 4 (8) ◽  
pp. 298-308
Author(s):  
Lidia Ait Ouahioune ◽  
Fatma Bara ◽  
Karim Bariz ◽  
Karim Houali ◽  
Djamel Djenane
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Phoenix Dactylifera ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phoenix Dactylifera L ◽  
Dpph Method

Background: Conventional food additives were associated with potential harm. Palm seeds are an interesting source of high-added value bioactive compounds, regarding their potential use in food industries. Aim: Date seeds extract (DSE) of Algerian Phoenix dactyliefra L. was studied for its phytochemical, antioxidant, and antibacterial activity and to identify its potential uses in foods. Material and methods: A total soluble solid of DSE (°Brix) was determined by refractometry. The total phenolic contents (TPCs) were determined according to the Folin-Cioclateu method and the total flavonoid and flavonols were also estimated with colorimetric method. The DPPH method was used to determine the antioxidant activity of DSE. The IC50 values were also calculated and the antioxidant activity index (AAI) was determined. The agar well diffusion method was employed for the antibacterial activity of the aqueous DSE against various pathogens. Results: Moisture, ash, and fat contents (%) determined were 11.06, 1.33, and 7.06, respectively. The physicochemical analysis of the extract showed a pH value of 4.67, titratable acidity value of 0.64 citric acid/100 g, °Brix value = 3%, sugars values of 0.12, 0.47, and 0.35 g/L for reducing sugar, total sugar, and sucrose, respectively. The determination of phenolic compounds was carried out on three groups, namely total phenols = 229.67 mg GAEq/g, flavonoids = 201.12 mg QEq/g, and flavonols = 173.03 mg QEq/g, respectively. The DSE demonstrates to be effective as an antioxidant “in vitro” and showed a strong antibacterial activity with different inhibition levels, depending on the bacterial strains. Conclusion: The current investigations present knowledge about the possible role of palm industry by-products as a novel perspective for the development of new foods. We suggest that date seeds can be exploited in some food applications utilizing their high levels of bioactive molecules. ​Keywords: Phoenix dactylifera L., Degla-Baïda, seeds extract, bioactive molecules, biological activity, in vitro, novel perspective.

Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Magdalena Woźniak ◽  
Lucyna Mrówczyńska ◽  
Anna Sip ◽  
Marta Babicka ◽  
Tomasz Rogoziński ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Pathogenic Bacteria ◽  
Radical Scavenging Activity ◽  
Colorimetric Method ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Content ◽  
Diffusion Method ◽  
Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

2021 ◽  
Vol 2 (1) ◽  
pp. 23-33
Author(s):  
Abdul Aziz ◽  
Veggy Nadya Yuliawan ◽  
Paula Mariana Kustiawan
Keyword(s):  
Antibacterial Activity ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Polar Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phytochemical Content ◽  
Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2  mm ± 1.5) at  200µg/mL concentration.

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF GLYCYRRHIZA GLABRA ROOT EXTRACTS

2020 ◽  
pp. 166-170
Author(s):  
SHUSHIL BHUSAL ◽  
KHAGA RAJ SHARMA
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Infections ◽  
Plant Secondary Metabolites ◽  
Glycyrrhiza Glabra ◽  
Antioxidant Potential ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Meoh Extract ◽  
Etoac Fraction

Objectives: The present study was designed to investigate the phytochemical analysis, antioxidant potential, and antibacterial activities of the traditionally used medicinal plant Glycyrrhiza glabra. Methods: The plant secondary metabolites were extracted through cold percolation using methanol (MeOH) as a solvent. The MeOH extract was further fractionated in different solvents in increasing order of polarity. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl assay. The antibacterial activity was studied by agar well diffusion method. Results: The antioxidant potential IC50 was found 43.13, 104.83, and 200.11 μg/ml for ethyl acetate (EtOAc), MeOH, and chloroform (CHCl3) extracts, respectively. The EtOAc fraction showed the potent antioxidant with IC50 43.13 μg/ml compared to the standard ascorbic acid 58.76 μg/ml. The antimicrobial activity exhibited by MeOH extract against Bacillus subtilis (ATCC 6051) and Staphylococcus aureus (ATCC 6538P) zone of inhibition was 18 mm and 17 mm, for chloroform extracts 15 mm and 13 mm, and for EtOAc fraction 11 mm against Bacillus subtilis. The highest dilution that yielded no single bacteria colony on the nutrient agar plates for Bacillus subtilis and S. aureus of MeOH extract was found 0.39 mg/ml and 6.25 mg/ml, for chloroform extract 3.125 mg/ml and 6.25 mg/ml and EtOAc fraction against Bacillus subtilis was 12.50 mg/ml as minimum bactericidal concentration. Conclusion: The plant extracts showed potent antioxidant and antibacterial activity. The results support for using the G. glabra in bacterial infection which provides partial scientific validation for using the plant against bacterial infections.

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