scholarly journals PHYSICOCHEMICAL, PHYTOCHEMICALS AND ANTIOXIDANT EVALUATION OF GUAZUMA ULMIFOLIA FRUIT

2018 ◽  
Vol 10 (9) ◽  
pp. 87
Author(s):  
Bhavani Duraiswamy ◽  
Malairajan Singanan ◽  
Vanitha Varadarajan
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Colorimetric Method ◽  
Water Soluble ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Fruit Extract ◽  
Guazuma Ulmifolia

Objective: The present study was designed to evaluate the physicochemical, qualitative, quantitative analysis and antioxidant activity of ethanol fruit extract of Guazuma ulmifolia. Studies were carried out to detect the bioactive component.Methods: The physiochemical properties such as loss on drying, extractive value for ethyl acetate, acetone, ethanol, total ash, acid insoluble ash, water-soluble ash and PH values; the phytochemical such as tannins, phenol, terpenoids, flavonoids, saponin, quinones, cardiac glycosides, coumarin, steroids and acids were found using standard methods. The total phenol and flavonoid content were estimated by Folinciocalteu and Aluminium chloride by colorimetric method. In vitro antioxidants, properties were evaluated by DPPH and FRAP assay.Results: Phytochemical analysis reveals the availability of secondary metabolites like tannins, phenol, terpenoids, flavonoids, coumarin, steroids, and cardiac glycosides in the different extract. The quantitative analysis for ethanolic fruit extract was done for total phenolic content (TPC), total flavonoids content (TFC). An ethanolic extract found to have high TPC [27.2797±0.1756] than TFC. Antioxidant activity was evaluated by DPPH assay (2, 2-diphenyl-1-picrylhydrazyl-hydrate) and FRAP (ferric Reducing Ability of Plasma).Conclusion: On the basis of this study, it was found that the Guazuma ulmifolia fruits have potential to act as a source of useful drugs due to the presence of various active phytochemical constituent, which will be helpful in preventing various diseases caused by the oxidative stress.

scholarly journals Phytochemical Analysis and In-vitro Antioxidant Activity of Aerial Parts of Trichodesma indicum

2020 ◽  
Vol 11 (2) ◽  
pp. 1386-1393
Author(s):  
Hamsalakshmi ◽  
Suresh Joghee ◽  
Akassh M
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Ethyl Acetate ◽  
Physicochemical Analysis ◽  
Water Soluble ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Aerial Parts

This study was conducted to assess the physicochemical analysis, qualitative and quantitative phytochemical analysis and antioxidant activity of various solvent (n-hexane, ethyl acetate, ethanol, and water) extract obtained from Trichodesma indicum aerial parts. The physicochemical analysis was carried out using standard reported methods. Successive solvent extraction was carried out using n-hexane, ethyl acetate, and ethanol by the Soxhlet method, followed by maceration with water. Qualitative phytochemical screening was done using standard procedures. The extracts were analyzed for its total phenolic and total flavonoid contents by Folin - ciocalteu and Aluminium chloride colorimetric methods. For the extracts, antioxidant activity was screened using three different methods, 1, 1-diphenyl-2-picryl-hydrazyl assay, Nitricoxide assay, and Superoxide dismutase assay. Ascorbic acid has been used for antioxidant activity as a positive control. The percentage of inhibition was measured at various levels of concentration. The total ash, water-soluble ash, acid insoluble ash, sulfated ash, and the moisture content of the powdered drug was found to be 15.30, 07.50, 03.99, 21.00, 05.00%w/w. The qualitative phytochemicals found in the extracts are carbohydrates, proteins, and amino acids, flavonoids, phenols, phytosterols, saponins, tannins, and glycosides. The total phenolic and total flavonoid content of the extracts was found in the sequence of ethyl acetate > n-hexane > ethanol > aqueous. All the extracts demonstrated significant concentration-dependent antioxidant activity in DPPH assay, Nitric oxide assay, and Superoxide dismutase assay. The findings indicated that all solvent extracts of Trichodesma indicum have promising antioxidant activity and that could be the presence of phytochemicals present in the extract.

scholarly journals Determination of total phenolic and flavonoid contents of jackfruit peel and in vitro antiradical test

Food Research ◽  
2020 ◽  
Vol 5 (1) ◽  
pp. 84-90
Author(s):  
Yamin ◽  
Ruslin ◽  
Mistriyani ◽  
Sabarudin ◽  
S. Ihsan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Colorimetric Method ◽  
Ethyl Acetate Fraction ◽  
Premature Aging ◽  
Natural Antioxidant ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Free radical is any molecular species that have unpaired free electrons in their outer orbital shell that make radicals highly reactive, resulting in pathogenesis conditions such as cellular injury, premature aging, cancer, hepatic disorders, neurodegenerative diseases, cardiovascular disease, and kidney disease. One source of natural antioxidant is jackfruit. The purpose of this research was to determine the phenolic and flavonoid contents in the extracts and fractions of jackfruit peel and their potential as antioxidants. Jackfruit peel powder was extracted from maceration. The total phenolic content was determined by the Folin-Ciocalteu method. Meanwhile, flavonoid content was determined using the aluminium chloride complex colorimetric method. Measurements of antioxidant activity were conducted using the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The ethyl acetate fraction had high phenolic and flavonoid contents, which were 49.667±1.508 g GAE/100 g of sample and 70.199±0.374 g of quercertin equivalent/100 g. The ethyl acetate fraction had the strongest antioxidant activity with IC50 value of 4.539±0.201 µg/ mL and correlation value (R2 ) of 0.5881 for phenols and R2 of 0.7241 for flavonoids. Ethyl acetate fraction of jackfruit peel is very potential to be developed as a natural antioxidant and functional food.

scholarly journals Phytochemical Analysis, Biochemical and Mineral Composition and GC-MS Profiling of Methanolic Extract of Chinese Arrowhead Sagittaria trifolia L. from Northeast China

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3025 ◽  
Author(s):  
Maqsood Ahmed ◽  
Mingshan Ji ◽  
Aatika Sikandar ◽  
Aafia Iram ◽  
Peiwen Qin ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Chemical Composition ◽  
Northeast China ◽  
Biochemical Analysis ◽  
Chemical Compounds ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Human Beings

Sagittaria trifolia is a medicinal foodstuff of China and East Asia belonging to the family Alismataceae. Samples of S. trifolia tubers were collected from Meihekow, Siping, Jilin, Harbin and Wuchang from Northeast China. The current study was aimed to evaluate the qualitative and quantitative analysis, antioxidant activity, biochemical analysis and chemical composition of different populations of S. trifolia. By using Folin–Ciocalteu, aluminium chloride colourimetric and 1,1-diphenyl-1-picrylhydrazyl (DPPH), total phenol and flavonoids content and antioxidant activity was analysed. Furthermore, chemical composition, biochemical analysis and mineral substances were also determined. The results showed the presence of flavonoids, phenols, saponins, tannins, glycosides and steroids except for alkaloids and terpenoids by qualitative analysis. Quantitative analysis revealed that highest total phenol, flavonoids content and antioxidant potential identified from Meihekow, i.e., 2.307 mg GAE/g, 12.263 mg QE/g and 77.373%, respectively. Gas chromatography-mass spectrometry results showed the presence of 40 chemical compounds corresponding to 99.44% of total extract that might be responsible for antioxidant properties. Mineral and biochemical analysis revealed the presence of calcium, magnesium, potassium, sodium, iron, copper, zinc and, carbohydrate, protein, fibre and fat contents, respectively. Interestingly, all S. trifolia populations collected from different locations possess similar composition. The dietary values, phytoconstituents, antioxidant activities and nutritional and curative chemical compounds of S. trifolia are beneficial for the nutritherapy of human beings.

scholarly journals Antioxidant activity of Clematis brachiata Thunb. leaf

2018 ◽  
Vol 53 (3) ◽  
pp. 185-190 ◽  
Author(s):  
M Mostafa ◽  
S Ahmed ◽  
AJ Afolayan
Keyword(s):  
Antioxidant Activity ◽  
Dry Matter ◽  
Methanol Extract ◽  
Total Phenolics ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Good Source ◽  
Diammonium Salt ◽  
Phenolic And Flavonoid

The antioxidant activity, total phenolic and flavonoid contents of different extracts of the Clematis brachiata Thunb leaves were determined. The antioxidant activity was evaluated using spectroscopic methods against 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2′-azinobis [3-ethylbenzothiazoline-6-sulphonic acid] diammonium salt radical cation (ABTS). Folin Ciocalteu method was used to determine the total phenolics and Aluminium Chloride Colorimetric method was used to determine the flavonoids contents in these extracts. The results showed that the methanol extract of the leaf exhibited the highest antioxidant activity with the value of 180.45 ±2.4 μg mL-1 in DPPH and 60 ±0.80 μg mL-1 in ABTS assay among the extracts. The methanol extract contains more phenolic compounds (178±2.20 mg/g as galic acid equivalent per g dry matter) and the acetone extract contains more flavonoids (135.11±1.20 mg/g as quercitin equivalent per g dry matter) among the extracts. This study provides the evidence that the leaves of the Clematis brachiata Thunb could be a good source of natural antioxidant.Bangladesh J. Sci. Ind. Res.53(3), 185-192, 2018

scholarly journals ANTIOXIDANT, ANTIMICROBIAL ACTIVITY AND TOTAL PHENOL AND FLAVONOIDS ANALYSIS OF SAMBUCUS NIGRA (ELDERBERRY)

2020 ◽  
pp. 35-37
Author(s):  
N. SRINIVAS GOUD ◽  
GOBIND PRASAD
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Pseudomonas Putida ◽  
Colorimetric Method ◽  
Total Phenol ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Sambucus Nigra ◽  
Fruit Extract

Objective: The goal of this study was paying attention on the chemical characterization of phytochemical compounds and their antioxidant activity of Sambucus nigra L. Methods: Phytochemical analysis was performed by Sambucus nigra L fruit extract. Total Phenol and Flavonoids content of elderberry fruit extract also determined using Folin-Ciocalteu colorimetric method and aluminum chloride colorimetric method. Antibacterial activity was performed by disk diffusion method and Antioxidant capacity was investigated by DPPH assay, butylhydroxytoluene used as a standard. Results: The richest anthocyanin in elderberry fruits was cyanidin-3-O-sambubioside. The antioxidant capacity obtained for elderberry extract proved that elderberry shown highest antioxidant activity, being the richest anthocyanins. The antioxidant capacity of elderberry fruit methanolic extract was recorded 62.56±1.12 percentages of scavenging activity. We also investigated antibacterial activity against four species Escherichia coli, Pseudomonas putida, Bacillus cereus, and Staphylococcus aureus. There E. coli was recorded 12.0 mm and Pseudomonas putida was recorded 0.34 mm zone of inhibition. Conclusion: The conclusion of our study is that Sambucus nisgra fruit extract has very high antioxidant activity which makes it recommendable for food industry and dietary supplement.

scholarly journals Phytochemical Content, Antibacterial and Antioxidant Potential of Endemic Plant Anabasis aretioïdes Coss. & Moq. (Chenopodiaceae)

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Souad Senhaji ◽  
Fatima Lamchouri ◽  
Hamid Toufik
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Endemic Plant

This study aims to investigate the biological activities of endemic plant Anabasis aretioïdes harvested in eastern Morocco. Various extracts were prepared from aerial part by aqueous and organic extraction using a Soxhlet and cold maceration. Preliminary phytochemical analysis was carried out on the powder and on the different extracts by standard phytochemical tests, and was confirmed by a quantitative analysis based on the determination of total polyphenols and cathechic tannins. Antioxidant activity was evaluated in vitro by five methods: H2O2, DPPH, ABTS, FRAP, and RP, and the antibacterial activity was carried out by disc diffusion method and the determination of MIC and MBC. Phytochemical screening revealed the presence of cathechic tannins, saponins, and sterols and quantitative analysis showed that Ethyl acetate extract presented the high level of phenolic and cathechic tannins contents (46.79 ± 0.75 µg GAE/mg E and 46.46 ± 0.67 µg CE/mg E). A highest hydrogen peroxide activity was observed in aqueous macerated extract (7.84 ± 0.44%) and the macerated methanol extract has the highest rates for the other four antioxidant activity tests: It was able to reduce DPPH with an IC50 of 52.91 ± 0.24 µg/ml, the highest ABTS•+ radical scavenging capacity (48.99 ± 1.316 µg TE/mg E), it showed also the highest antioxidant activity by the FRAP and reducing power test (99.73 ± 3.570 µg TE/mg E and 72.176 ± 0.540 µg AAE/mg E). Antibacterial screening showed that the maximum zone of inhibition was noted for ethyl acetate extract against Staphylococcus aureus (13.5 mm). The lowest MIC value was obtained with methanolic and macerated methanolic extracts against Protéus mirabilis strain (MIC = 3.125 mg/ml). Principal component analysis showed that the four methods ABTS, DPPH, FRAP, and RP are highly correlated and a correlation between the antioxidant activity and the total phenolic contents of the extracts indicated that phenolic compounds were the dominant contributors to the antioxidant activity of the plant.

Total phenolic content and antioxidant activity of plants used in traditional Romanian herbal medicine

2011 ◽  
Vol 6 (3) ◽  
pp. 388-396 ◽  
Author(s):  
Iuliana Spiridon ◽  
Ruxanda Bodirlau ◽  
Carmen-Alice Teaca
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Total Antioxidant Activity ◽  
Origanum Vulgare ◽  
Total Antioxidant

AbstractA number of herbal plants from Romania widely used as natural food additives or for health promotion in traditional medicine were investigated for their antioxidant activity. Methanol extracts were obtained from plants belonging to the Lamiaceae family (lavender Lavandula angustifolia L.; lemon balm Melissa officinalis; sage Salvia officinalis; oregano Origanum vulgare L.; rosemary Rosmarinus officinalis L.; thyme Thymus vulgaris L.; mullein Verbascum phlomoides; mint Mentha longifolia), Clusiaceae family (St John’s wort Hypericum perforatum L.), and Compositae family (elecampane Inula helenium). Total phenolic concentration was determined using the Folin-Ciocalteu phenol reagent method, while total flavonoids were measured using the aluminium chloride colorimetric method. Relationships between total antioxidant activity and composition of plant extracts were evaluated. Origanum vulgare extract showed the highest antioxidant activity and total phenolic content compared to the other plants extracts. A positive correlation was observed between total antioxidant activity and total phenolic content of the analyzed extracts.

scholarly journals Pharmacognostic Standardization, Phenolic and in Vitro Antioxidant Activity of Silene villosa (Family: Caryophyllaceae)

2017 ◽  
Vol 9 (3) ◽  
pp. 106 ◽  
Author(s):  
Ahmed I. Foudah
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Property ◽  
Water Soluble ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Microscopic Features

The present study was designed to explore some of the quality principles and <em>in vitro</em> antioxidant exploration of <em>Silene villosa</em> “a non-intentional” herb. The pharmacognostical study and phytochemical evaluation of the herbs of <em>S. villosa</em> was carried out following the reported methods. The total phenolic contents and antioxidant potential of the methanol extract was also evaluated applying the standard methods. Microscopic features of the Transverse Section (T.S) of the root were observed as epidermis, medullary rays, xylem cells, phloem cells. The microscopic study of the powder showed the presence of spiral vessels, lignified fibre or tannin containing cells. The extractive value showed the water soluble materials were higher than the alcohol soluble materials. Primarily phytochemical analysis and total phenolic contents (11.78±0.169 %) in powder and extract respectively were conducted. The methanol extract of <em>S. villosa</em> showed dose dependent antioxidant activity. The present study helps in authentication of <em>S. villosa</em> and enhances further exploration of the health benefits due to the presence of phenolic contents and antioxidant property.

scholarly journals Physicochemical, Qualitative and Quantitative Phytochemical Analysis of the Leaf and Bark of Bombax Ceiba L (Red Silk Cotton Tree)

2018 ◽  
Vol 8 (6-s) ◽  
pp. 105-110
Author(s):  
Sarita Karole ◽  
Girendra Gautam ◽  
Shailesh Gupta
Keyword(s):  
Quantitative Analysis ◽  
Manufacturing Industry ◽  
Hiv Infections ◽  
Morphological Characters ◽  
Aluminium Chloride ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Test Protocol ◽  
Qualitative And Quantitative

Bombax ceiba L is a big deciduous tree found in tropical and subtropical regions of Asia, Africa and Australia. Conventional systems of medicine such as Ayurveda, Siddha and Unani have been highlighted the use of B. ceiba parts (bark, leaves and flower) for the treatment of manydiseases like hypertension, HIV infections, inflammation, catarrhal affection, ulcer, acne, gynecological disorders, fever, dysentery, algesia, hepatotoxicity,  piles and urinary infections. The aim of the present study was to evaluate pharmacognostic, physicochemical, qualitative and quantitative phytochemical analysis of leaf and bark of B. ceiba collected from Bhopal region of Madhya Pradesh. The pharmacognostic studies out in terms of various investigations like organoleptic or morphological characters, microscopic or anatomical studies, physicochemical evaluations (loss on drying, ash value, extractive values and acid insoluble ash value). Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenolics and flavonoids were determined by the well-known test protocol available in the literature. The detail microscopy of bark revealed the presence of cork cell, lignified fibre, calcium oxalate crystals, xylem vessels. Physiochemical parameters such as percentage of foreign matters, ash values, loss on drying swelling index, extractive values were determined. Phytochemical analysis revealed the presence of phenols, flavonoids, tannins, alkaloids and glycosides.  In the procedure of quantitative analysis of flavonoids and phenolic   compound was carried out by aluminium chloride Folins and Ciocalteau reagent method .In this methodthe totalflavonoids content and phenolic content of B. ceiba ethanolic and aqueous barks extracts was found to be 6.272, 3.363 and 2.607, 1.607mg /100mg respectively and total phenolic and flavonoids content of B. ceiba ethanolic, aqueous leaves extracts was found to be 7.381, 4.590 and 3.200, 1.792 mg/100mg respectively. These information will also be helpful to differentiate B. ceiba from the closely related other species. The diverse array of phytochemicals present in the plant thus suggests its therapeutic potentials which may be explored in drug manufacturing industry as well as in traditional medicine.

scholarly journals HPLC-UV profile of Genista ulicina Spach. (Fabaceae) extracts and in vitro antioxidant activity

2016 ◽  
Vol 8 (3) ◽  
pp. 398 ◽  
Author(s):  
Kaoutar Chebbah ◽  
Eric Marchioni ◽  
Djamel Sarri ◽  
Ahmed Menad ◽  
Ratiba Mekkiou ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Detector ◽  
Etoac Extract ◽  
Phenolic And Flavonoid ◽  
Β Carotene

<p>To perform a qualitative and quantitative analysis of the phenolic and flavonoid contents and evaluate the antioxidant activity of ethyl acetate (EtOAc) and <em>n</em>-butanol (<em>n</em>-BuOH) extracts of the aerial parts of <em>Genista ulicina </em>Spach. from Algeria.<strong> </strong>The qualitative analysis of plant extracts was carried out by RP-HPLC using UV detector, whereas the quantification of total phenolic and flavonoid contents was completed according to the Folin-Ciocalteu procedure and aluminium chloride colorimetric method respectively. To evaluate the extract's antioxidant activity, Two in vitro antioxidant tests were employed: DPPH and β-carotene bleaching assay. The HPLC/DAD chromatogram showed several peaks indicating the presence of phenolic acids, flavonoids and isoflavonoids in both extracts. The total phenolic content (TPC) ranged from 62.56 and 50.45 mgGAE/g extract, while the total flavonoids content varied between 53.1 and 48.4 mgQE/g extract for EtOAC and <em>n</em>-BuOH respectively. EtOAc extract showed a maximum inhibition value (78.15%) at 150µg/mL using DPPH test and highest antioxidative power (82.42%) using β-carotene bleaching assay comparing with standards. The HPLC-UV analysis showed the richeness of both extracts in phenolic and flavonoid contents. The EtOAc<em> </em>extract exhibited good antioxidant activities comparing to the <em>n</em>-BuOH extract. Thus <em>Genista ulicina</em> could be indicated as a plant of phytopharmaceutical importance.<strong></strong></p>

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