scholarly journals DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR STANDARDIZATION OF A NOVELMUCILAGE OBTAINED FROM OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH) FRUIT

2021 ◽  
pp. 28-41
Author(s):  
SOMA DAS ◽  
ANANYA GHOSH ◽  
RUSHAM DAS ◽  
GOURANGA NANDI ◽  
LAKSHMI KANTA GHOSH
Keyword(s):  
Quality Control ◽  
Ascorbic Acid ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Thin Layer ◽  
High Performance ◽  
Control Parameters ◽  
Fruit Extract ◽  
Quality Control Parameters ◽  
Layer Chromatography

Objective: The main objective was to focus on qualitative and quantitative analysis of isolated okra mucilage by High-performance Thin Layer Chromatography to set up the quality control parameters for the isolated mucilage. Methods: High-performance thin-layer chromatography (HPTLC) were applied for the identification of components present in methanolic and ethanolic fruit extract of okra at 254 nm and 356 nm. Quantitative analysis of amino acid ascorbic acid and total polyphenol content was determined. Results: The results showed that the yield percentage for methanolic and ethanolic fruit extract of the okra fruit mucilage was found to be 13.5 and12.5% respectively. HPTLC determination of methanolic and ethanolic okra fruit extract showed the presence of 8 components with Rf values in the range of 0.14 to 0.62 and 0.14 to 0.54 respectively when detected at wavelengths 254 nm and at 356 nm. The total amino acid in okra fruit methanolic extract was found to be 11.45%w/w. The ascorbic acid content in methanolic okra fruit extract and ethanolic okra fruit extract was found to be 0.24 %w/w and 0.18% w/w respectively. The total phenolic contents (tannic acid equivalents, mg/g) in the methanolic and ethanolic okra fruit extracts were calculated to be 4.6 % w/wand 5.3% w/w respectively. Conclusion: The data revealed dual benefits like it can act as a potential novel functional ingredient with health-promoting application due to the presence of ascorbic acid and total phenolics contents and at the same time the data provided guidelines for quality control parameters for the isolated okra fruit

scholarly journals Quantitative Analysis of Sulpiride and Impurities of 2-Aminomethyl-1-Ethylpyrrolidine and Methyl-5-Sulphamoyl-2-Methoxybenzoate in Pharmaceuticals by High-Performance Thin-Layer Chromatography and Scanning Densitometry

1999 ◽  
Vol 82 (4) ◽  
pp. 825-829 ◽  
Author(s):  
Danica Agbaba ◽  
Tatjana Miljkovic ◽  
Valentina Marinkovic ◽  
Dobrila Zivanov-Stakic ◽  
Sote Vladimirov
Keyword(s):  
Quantitative Analysis ◽  
Thin Layer ◽  
High Performance ◽  
Methylene Chloride ◽  
Solvent System ◽  
Ammonia Solution ◽  
Dosage Forms ◽  
Raw Material ◽  
Layer Chromatography

Abstract A simple and reliable thin-layer chromatographic method for determining sulpiride and impurities of 2-aminomethyl-1-ethylpyrrolidine and methyl-5-sulphamoyl-2-methoxybenzoate was developed and validated. A methylene chloride–methanol–ammonia solution (25%; 18 + 2.8 + 0.4, v/v) solvent system is used for separation and quantitative evaluation of chromatograms. The chromatographic plate is first scanned at 240 nm to locate chromatographic zones corresponding to sulpiride and methyl-5-sulphamoyl-2-methoxybenzoate. Then 2-aminomethyl-1-ethylpyrrolidine is derivatized in situ with ninhydrin, and resulting colored spots are measured at 500 nm. The method is reproducible and convenient for quantitative analysis and purity control of sulpiride in its raw material and in its dosage forms.

scholarly journals Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

2019 ◽  
Vol 102 (3) ◽  
pp. 726-733 ◽  
Author(s):  
Wan-Xin Zhang ◽  
In-Cheng Chao ◽  
De-jun Hu ◽  
Farid Shakerian ◽  
Liya Ge ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Quality Control ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Aerial Parts ◽  
Alpinia Officinarum ◽  
Layer Chromatography ◽  
Different Parts ◽  
Tof Ms

Abstract Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

scholarly journals Separation of 9-Fluorenylmethyloxycarbonyl Amino Acid Derivatives in Micellar Systems of High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Beata Polak ◽  
Adam Traczuk ◽  
Sylwia Misztal
Keyword(s):  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Amino Acid Derivatives ◽  
Solute Retention ◽  
Micellar Systems ◽  
Layer Chromatography

AbstractThe problems with separation of amino acid mixtures in reversed-phase mode are the result of their hydrophilic nature. The derivatisation of the amino group of mentioned above solutes leads to their solution. For this purpose, 9-fluorenylmethoxycarbonyl chloroformate (f-moc-Cl) as the derivatisation reagent is often used. In our study, the separation of some f-moc- amino acid derivatives (alanine, phenylalanine, leucine, methionine, proline and tryptophan) with the use of micellar systems of reversed-phase high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) is investigated. The effect of surfactant concentration, its type (anionic, cationic and non-ionic) and mobile phase buffer pH on the discussed above solute migration distances are presented. Our work reveals that the increase of sodium dodecylsulphate concentration in the mobile phase has a different effect on solute retention in HPTLC and PPEC. Moreover, it also affects the order of solutes in both techniques. In PPEC, in contrast to the HPTLC technique, the mobile phase pH affects solute retention. The type of surfactant in the mobile phase also impacts solute retention and migration distances. A mobile phase containing SDS improves system efficiency in both techniques. Herein, such an effect is presented for the first time.

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