scholarly journals SUB-ACUTE TOXICITY STUDY OF ETHANOLIC EXTRACT OF PACING (COSTUS SPECIOSUS) IN MALE MICE

2016 ◽  
Vol 8 (12) ◽  
pp. 97 ◽  
Author(s):  
Ika Puspita Sari ◽  
Arief Nurrochmad
Keyword(s):  
Acute Toxicity ◽  
Research Result ◽  
Ethanolic Extract ◽  
Male Mice ◽  
Glucose Levels ◽  
Costus Speciosus ◽  
Blood Glucose Levels ◽  
Acute Toxicity Study ◽  
Food And Drink ◽  
Significant Disturbance

<p><strong>Objective</strong>:<strong> </strong>The aim of this research was to evaluate the safety of <em>Costus speciosus</em> extract (CSE) after a 90-day administration in male mice.</p><p><strong>Methods</strong>:<strong> </strong>Sub-acute toxicity test was performed using the OECD 408 method. CSE at 275-1100 mg/kg/day was administered to male mice for 90 d. The appearance of toxic symptoms was observed every day, followed by measurement of food and drink intakes. Change in body weight, the result of a routine blood check and blood biochemistry were observed and statistical analysis was performed on them. Test animals were killed at the end of the study and the weights of vital organs were examined before organ histology was examined.</p><p><strong>Results</strong>:<strong> </strong>The research result showed that the administration of CSE at 275-1100 mg/kg/day for 90 d did not show any significant disturbance in all parameters, except for reductions of cholesterol and blood glucose levels of test animals (p&lt;0.05).</p><p><strong>Conclusion</strong>:<strong> </strong>The results obtained in this research indicated the safety of CSE as a candidate of standardized herbal medicine for male contraception.</p>

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Ketoksikan Akut dari Ekstrak Etanolik Daun Jarak Pagar (Jatropa curcas) pada Mencit Jantan Galur Balb/C

2014 ◽  
Vol 15 (1) ◽  
pp. 52
Author(s):  
Hanif Nasiatul Baroroh ◽  
Eka Prasasti Nur Rachmani
Keyword(s):  
Acute Toxicity ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Mice ◽  
Animal Test ◽  
Group I ◽  
Liver And Kidney ◽  
Single Dosage ◽  
Histopathology Examination ◽  
Lung And Kidney

The acute toxicity of Jatropa curcas leaves on Balb/C male mice was studied in rats. This research aimed to determine acute toxicity, evaluate spectrum of toxic effect and mechanism that caused the death of animal test after administration of ethanolic extract of J. curcas leaves, single dosage orally on 24 hours observation. The research used male mice, which are divided into 5 groups. Group I was negative control with CMC-Na. Group II, III, IV, and V were given extract with dose of 1400 mg/kgBW, 2240 mg/kgBW, 3584 mg/kgBW and 5734 mg/kgBW, respectively. Evaluation of the toxic symptoms and death of animal test was done for 24 hours. If the animal test was died before 24 hours then it underwent surgery to take the heart, liver, lung, and kidney. In the end of the evaluation, all mice were killed to take the vital organs for histopathologic examination. No mortality was observed during study. The test resulted LD50 of ethanolic extract from J. curcas leaves using Balb/C male mice was 5734 mg/kg of BW. It was categorized as practically not toxic. Administration of the extract did not cause alterations of animal behaviours. Histopathology examination shows inflammation in lung, liver, and kidney after administration of the extract.

scholarly journals Comparative evaluation of anti-diabetic action and pancreatic histopathology of rats treated with two alkaloidal plant extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1841-1846
Author(s):  
Bonagiri Sreedevi ◽  
Vijaya Kuchana ◽  
Shobharani S
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Standard Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

This study aimed to understand Strychnosnuxvomica and Holarrhena pubescens Stem bark extract action towards M3 receptor in controlling blood glucose levels. Strychnos nux vomica  and Holarrhena pubescens are both alkaloidal drugs can help in controlling Hyperglycemic level. This will be useful in the formulation of a new herbal drug molecule for treating diabetes. Chloroform and ethanolic extracts of selected alkaloidal plants were extracted using the soxhlet apparatus and obtained quotes were tested for acute toxicity studies and carried out anti-diabetic action on Wister albino rats for 21 days. Results obtained from Blood glucose levels and histopathological study of test groups are compared with blood glucose levels of standard group, and highly significant action was identified by the chloroform extract of Strychnos nux vomica and Holarrhena pubescens group. Moderate anti-diabetic action was observed remaining two groups of ethanolic extracts. Strychnos nux vomica and Holarrhena pubescens ethanolic extract groups are acting on M3 receptors and controlling Hyperglycemic levels.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Taxithelium napalense acts against free radicals and diabetes mellitus

2017 ◽  
Vol 12 (2) ◽  
pp. 16 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Kishore Naidu Killari ◽  
Vedula Girija Sastry ◽  
Alekhya Ketha
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Video Clip ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Radical Scavenging Properties ◽  
Histopathological Studies

<p class="Abstract">In this work, <em>Taxithelium nepalense</em> ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the <em>T. nepalense</em> has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 50 sec   <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a>   <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a></p>

scholarly journals ACUTE TOXICITY STUDY OF THE LEAVES ETHANOLIC EXTRACT OF PICRIA FEL-TERRAE LOUR.

2018 ◽  
Vol 11 (13) ◽  
pp. 55
Author(s):  
Popi Patilaya ◽  
Dadang Irfan Husori ◽  
Imam Bagus Sumantri ◽  
Simon Sihombing
Keyword(s):  
Acute Toxicity ◽  
Plant Extract ◽  
Aspartate Aminotransferase ◽  
Alanine Aminotransferase ◽  
Ethanolic Extract ◽  
Male Mice ◽  
Plant Leaves ◽  
Liver And Kidney

 Objective: Picria fel-terrae belongs to family Linderniaceae is also known as Pugun tano by Indonesian people. The ethanolic extract of plant leaves has several potential pharmacological activities including antidiabetic, anthelmintic, and antioxidant. However, the toxicity of the plant extract is rarely explored. This work was to investigate toxicity of the leaf ethanolic extract of P. fel-terrae on Artemia salina and male mice.Methods: Acute toxicity of the plant extract was studied by in vitro and in vivo methods. In vitro study was carried out by exposing nauplii to the plant extract at concentrations of 10, 100, 200, 500, and 1000 μg/ml for 48 h. In vivo study was performed on male mice that divided into four groups. Groups I, II, III, and IV were treated with sodium carboxymethyl cellulose 0.5%, the ethanolic extract of plant leaves at doses of 1000, 2000, and 5000 mg/kg bw, respectively. The animal toxic symptoms were observed every day for 14 days. On day 15, the blood of mice was collected to measure alanine aminotransferase, aspartate aminotransferase, and creatinine levels. The effects of plant extract on vital animal organs such as heart, liver, and kidney were also studied. Statistical analysis of data was performed using analysis of variance and followed by Tukey post hoc.Results: The results showed that the leaf ethanolic extract of P. fel-terrae to have weakly toxicity on A. salina with the LC50 of 768.07 μg/ml. At in vivo studies, the toxic symptoms of mice were not identified during experiment with all doses of the plant extract for 14 days. In addition, aspartate aminotransferase and creatinine levels were no significantly different between control and all treatment groups (p>0.05). However, alanine aminotransferase level changed when mice were exposed by the plant extract at the doses of 2.000 and 5.000 mg/kg bw. Although the mice were not dead during experiment, the animal organs such as heart, liver, and kidney were histologically changed.Conclusion: This study suggests that the ethanolic extract of P. fel-terrae leaves has weakly toxicity on A. salina and causes histological changes on male mice organs at the high doses.

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