scholarly journals CYTOTOXIC EFFECT OF CRUDE EXTRACT AND FRACTION FROM CALOTROPIS GIGANTEA LEAVES ON HUMAN COLON CANCER WIDR CELL LINES

2016 ◽  
Vol 9 (1) ◽  
pp. 83 ◽  
Author(s):  
Roihatul Mutiah ◽  
Sukardiman . ◽  
Aty Widyawaruyanti
Keyword(s):  
Colon Cancer ◽  
Ethyl Acetate ◽  
Crude Extract ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Water Fraction ◽  
Widr Cell

<p><strong>Objectives: </strong>This paper sought to understand and determine the cytotoxic’s effects of crude extract and its fraction from <em>Calotropis gigantea</em> leaves on human colon cancer WiDr cell lines.</p><p><strong>Methods: </strong>The ethanolic extract was fractionated gradually with certain substances to yield four fractions. The substances were dichloromethane, ethyl acetate, and butanol. The four fractions resulted in dichloromethane fraction, ethyl acetate fraction, butanol fraction, and a water fraction. These fractions were then investigated for their cytotoxic effects on WiDr cells. The cell viability was assessed using MTT colorimetric assay.</p><p><strong>Results: </strong>The result indicated that the cytotoxic effects of the ethanolic extract (IC<sub>50</sub>48.5 μg/ml), ethyl acetate fraction (IC<sub>50</sub>41.79 μg/ml), and dichloromethane fraction (IC<sub>50</sub>40.57μg/ml) produced a much more potent effect than the butanol fraction (IC<sub>50</sub> 737.74 μg/ml) and water fraction (IC<sub>50</sub>8493 μg/ml).</p><p><strong>Conclusion: </strong>The ethanolic extract, ethyl acetate fraction and dichloromethane fraction exhibited a potent cytotoxic effect on human colon cancer WiDr cell line. The crude extract and fractions are potential to be developed as an anticancer agent in colon cancer therapy.</p>

scholarly journals Cytotoxic Activities of (Graptophyllum pictum (L.) Griff) Ethanolic Extract and Its Fractions on Human Colon Cancer Cell WiDr

2020 ◽  
Vol 25 (1) ◽  
pp. 29
Author(s):  
Azizah Amin ◽  
Andayana Puspitasari Gani ◽  
Retno Murwanti
Keyword(s):  
Colon Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Ethanolic Extract ◽  
Human Colon ◽  
Cytotoxic Activities ◽  
Human Colon Cancer ◽  
Water Fraction ◽  
Cancerous Cell ◽  
Ic50 Values

Colon cancer is the third most common cancer in the world. Treatments for colon cancer might cause major side effects, hence increase opportunities for the development of new cancer drugs. One of the plants that potential to develop as an anticancer agent is daun ungu (Graptophyllum pictum (L.) Griff). From previous studies, G.pictum had cytotoxic activity against several cancerous cell lines. Traditionally, G.pictum leaves have been used for hemorrhoid treatment. The purpose of this study was to determine the cytotoxic activity of G.pictum ethanolic extract and its fractions on human colon cancer WiDr cells and to elucidate the compounds contained in most active extracts/fractions. G.pictum was extracted using 70% ethanol and fractionated using n-hexane, chloroform, and ethyl acetate, respectively. The yield of the extract obtained was 18.9%. The yield of hexane, chloroform, ethyl acetate, and ethanol-water fractions were 1.07%, 2.93%, 10.26%, and 84.82%, respectively. The cytotoxic activity was carried out on WiDr cells using the MTT assay. Cytotoxic activity was determined based on IC50 values. IC50 value of extract, hexane, chloroform, ethyl acetate and ethanol-water fraction obtained on WiDr cells were 1527,58; 143,97; 507,19; 3538,67 and 3186,60 μg/mL. The hexane fraction containing terpenoids and phenolics showed the highest cytotoxic activities against WiDr colon cancer cells.

Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: A potential natural anti-cancer substance

2009 ◽  
Vol 276 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Masahiko Imai ◽  
Hidetomo Kikuchi ◽  
Takanori Denda ◽  
Kunio Ohyama ◽  
Chieko Hirobe ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Line ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Anti Cancer

scholarly journals Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

2015 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Nyi Mekar Saptarini ◽  
Irma Erika Herawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Ficus Benjamina ◽  
Water Fraction ◽  
Free Radical Formation ◽  
Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as <em>Ficus benjamina </em>and <em>Annona reticulata</em>. The study aimed to compare the antioxidant activity of extracts and fractions of <em>Ficus benjamina </em>and <em>Annona reticulata</em> leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC<sub>50</sub> values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC<sub>50</sub> value of <em>Ficus benjamina </em>was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC<sub>50</sub> value of <em>Annona reticulata</em> was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the <em>Ficus benjamina</em> water fraction was the best antioxidant compared to other extract and fraction.

Comparative Antioxidant Activity on The Ficus benjamina and Annona reticulata Leaves

2015 ◽  
Vol 4 (1) ◽  
pp. 21
Author(s):  
Nyi Mekar Saptarini ◽  
Irma Erika Herawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Natural Antioxidants ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Ficus Benjamina ◽  
Water Fraction ◽  
Free Radical Formation ◽  
Annona Reticulata

Antioxidants can prevent free radical formation. Natural antioxidants found in many plants, such as <em>Ficus benjamina </em>and <em>Annona reticulata</em>. The study aimed to compare the antioxidant activity of extracts and fractions of <em>Ficus benjamina </em>and <em>Annona reticulata</em> leaves against 1,1-diphenyl-2-picrilhydrazyl. The steps of this study consist of extraction, fractionation with n-hexane, ethyl acetate and water, phytochemical screening, antioxidant activity determination, and comparing the IC<sub>50</sub> values. Percentage scavenging activity of the extracts and fractions against DPPH was calculated to determine the antioxidant activity. The IC<sub>50</sub> value of <em>Ficus benjamina </em>was 127.86 ppm for ethanolic extract, 94.01 ppm for water fraction, 115.48 ppm for ethyl acetate fraction, and 335.50 ppm for n-hexane fraction. The IC<sub>50</sub> value of <em>Annona reticulata</em> was 274.31 ppm for ethanolic extract, 211.42 ppm for water fraction, 367.91 ppm for ethyl acetate fraction, and 741.08 ppm for n-hexane fraction. The results showed that the <em>Ficus benjamina</em> water fraction was the best antioxidant compared to other extract and fraction.

The Flavonol Isorhamnetin Exhibits Cytotoxic Effects on Human Colon Cancer Cells

2010 ◽  
Vol 58 (20) ◽  
pp. 10869-10875 ◽  
Author(s):  
Sara Jaramillo ◽  
Sergio Lopez ◽  
Lourdes M. Varela ◽  
Rocio Rodriguez-Arcos ◽  
Ana Jimenez ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Human Colon Cancer Cells

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

scholarly journals Morphomolecular identification, metabolic profile, anticancer, and antioxidant capacities of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 isolated from Egyptian remote cave

2021 ◽  
Author(s):  
Waill A. Elkhateeb ◽  
Walaa S. A. Mettwally ◽  
Shireen A. A. Saleh ◽  
Walid Fayad ◽  
Ibrahim M. Nafady ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Colon Cancer ◽  
Ethyl Acetate ◽  
Human Breast Cancer ◽  
Mcf7 Cell ◽  
Human Colon ◽  
Human Breast ◽  
Human Colon Cancer ◽  
Breast Cancer Mcf7 Cell

Abstract Searching for remote locations to screen for microorganisms, identify their metabolites, and investigate their bioactivities against lethal diseases such as cancer is of critical importance. In the current study, two fungal strains where isolated from a remote cave in Asyut governorate, Egypt. These isolates were morphologically and molecularly identified through sequencing their ITS region as Penicillium sp. NRC F1, and Penicillium sp. NRC F16. Investigating the metabolic profiles of the silylated ethyl acetate extracts of these fungi through conducting GC-Ms analysis revealed presence of 114 compounds belonging to different chemical classes. On the other hand, studying the in vitro bioactivity of both extracts showed moderate antioxidant activities. Penicillium sp. NRC F1 extract exhibited higher DPPH scavenging activity (74.41 ± 0.59%) at concentration of 200 µg/ml, in comparison with that exerted by Penicillium sp. NRC F16 extract at the same concentration (65.58 ± 1.55%). Moreover, studying the cytotoxicity of extracts against human colon cancer (HCT116), and human breast cancer (MCF7) cell lines revealed that cytotoxicity of both extracts was dose dependent. Promising cytotoxic effect was achieved against human colon cancer HCT116 using 200 µg/ml of Penicillium sp. NRC F1 extract (95.72 ± 1.13 % cytotoxicity), while Penicillium sp. NRC F16 ethyl acetate extract caused a cytotoxicity of 95.43 ± 1.4 %. Similarly, investigating the in vitro cytotoxicity of the extracts against human breast cancer MCF7 cell line resulted in observing promising activity of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 extracts, and they exhibited 97.29 ± 0.61 %; and 97.08 ± 1.07 % cytotoxicity, respectively. Results from this study nominate those strains as promising isolates and encourage for conducting further in vivo investigations to evaluate their potency.

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