scholarly journals IN VITRO ANTIOXIDANT CAPACITIES AND ANTIDIABETIC PROPERTIES OF PLUCHEA LEAVES AND GREEN TEA MIXTURES AT VARIOUS PROPORTIONS

2017 ◽  
Vol 9 (8) ◽  
pp. 203 ◽  
Author(s):  
Paini Sri Widyawati ◽  
Yesiana D. W. Werdani ◽  
Christine Setiokusumo
Keyword(s):  
Free Radical ◽  
Green Tea ◽  
Reducing Power ◽  
Alpha Amylase ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Graph Pattern ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Objective: This study was done to determine the effect of pluchea leaves and green tea mixtures at various proportions to antioxidant and antidiabetic properties.Methods: The research used a single factor randomized block design. Research factors were pluchea and green tea mixture proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w), respectively. Each of factors was repeated five times. The parameters observed in this study were phytochemical compounds, total phenols, total flavonoids, free radical DPPH scavenging activity, iron ion reducing power, alpha amylase and alpha glycosidase inhibition activities. The data were statistically α = 5%, if the data analysis showed a significant analyzed using analysis of variance (ANOVA) at effect then it was continued with duncan's multiple range test (DMRT).Results: The phytochemical compounds containing in pluchea leaves and green tea mixtures were alkaloids, flavonoids, phenolics, saponins, tannins, triterpenoids, sterols, and cardiac glycosides. The qualitative assay of phytochemical compounds from aqueous extract had a different pattern with total phenolic content (TPC) and total flavonoid content (TFC), especially at 75:25; 50:50 and 25:75 % (w/w) from pluchea leaves and green tea mixture proportions. The 50:50% (w/w) proportions of samples had the highest TPC and TFC, it was proposed that there was an interaction between bioactive compounds of pluchea leaves and green tea. TPC and TFC were correlated with free radical DPPH scavenging activity, iron reducing power and alpha glycosidase inhibition activity, but the alpha amylase inhibition activity had the different graph pattern with the TPC and TFC.Conclusion: The antioxidant capacity of pluchea leaves and green tea mixtures at various proportions had the graph pattern similar to the alpha glycosidase inhibitory activity and different with the alpha amylase inhibitory activity.

scholarly journals PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY

2020 ◽  
pp. 55-58 ◽  
Author(s):  
YOHANES TANDORO ◽  
PAINI SRI WIDYAWATI ◽  
TARSISIUS DWI WIBAWA BUDIANTA ◽  
GRACE SUMARGO
Keyword(s):  
Antioxidant Activity ◽  
Cardiac Glycosides ◽  
Reducing Power ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Passiflora Foetida ◽  
Dpph Scavenging

Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract. Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm. Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base. Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.

scholarly journals Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound

2019 ◽  
Vol 30 (4) ◽  
pp. 240
Author(s):  
Dela Vrianty ◽  
Rismawati Laila Qodariah ◽  
Wahyu Widowati ◽  
Ade Putra Fratama Sinaga ◽  
Dewi Fibrina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
Final Concentration ◽  
Ananas Comosus ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Tyrosinase Enzyme ◽  
Dpph Scavenging

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.

Characterization of non polar-polar solvent extracts from some tea plantation shade tree leaves with special reference to antioxidant and antibacterial activities

2020 ◽  
pp. 15-21
Author(s):  
Arindam Ghosh ◽  
Soumya Majumder ◽  
Sumedha Saha ◽  
Malay Bhattacharya
Keyword(s):  
Cardiac Glycosides ◽  
Polar Solvent ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Shade Trees ◽  
Crude Fibre ◽  
Shade Tree ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively.  Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water,  hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.

scholarly journals Ganoderma lucidum Metanolik Ekstraktının Fitotoksik Etkilerinin ve Antioksidan Potansiyelinin Araştırılması

2016 ◽  
Vol 4 (3) ◽  
pp. 163 ◽  
Author(s):  
Fuat Bozok ◽  
Tülin Eker ◽  
Gökhan Sezer ◽  
Adnan Bozdoğan ◽  
Hasan Hüseyin Doğan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ganoderma Lucidum ◽  
Methanolic Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Phytotoxic Effect ◽  
Dpph Scavenging Activity ◽  
Allelopathic Effects ◽  
Dpph Scavenging

The aim of this study is to reveal allelopathic effects and antioxidant activities of methanolic extract of Ganoderma lucidum in Zorkun plateau (Osmaniye). It was determined that methanolic extract of G. lucidum has significantly phytotoxic effect by increasing the doses (1, 2, 4, 8 mg/mL) on Hordeum vulgare and Triticum aestivum. Total phenol and flavonoid amounts of G. lucidum were 114.55 mg/kg and 8.95 mg/kg, respectively. 2,2-diphenyl-1-picrylhydrazyl (DPPH), scavenging activity (60%, 91% and 92%), reducing power (0.83, 1.43 and 2.23 Abs), and nitric oxide (NO) scavenging activity (48%, 55% and 70%) of this mushroom at the different concentrations (1.25, 2.5 and 5 mg/mL) were determined, respectively. However, H2O2 scavenging activity of the different concentrations (0.0156-0.125 mg/mL) was found as 18%, 56%, 60% and 86%, respectively. Based on these findings, it can suggest that G. lucidum methanolic extract has significantly phytotoxic effect and antioxidant activity. The present study is the first report on the phytotoxic effects and antioxidant activities of G. lucidum which consumed and collected from Amanos Mountains.

scholarly journals Antioxidant and Anti-tyrosinase Activities of Aloe vera Rind and Gel Extracts

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Sonya Bonifacia Mahadi ◽  
Rr. Anisa Siwianti Handayani ◽  
Wahyu Widowati ◽  
Wilsen Wilsen ◽  
Yunita Dewani ◽  
...  
Keyword(s):  
Free Radicals ◽  
Biomedical Research ◽  
Aloe Vera ◽  
The Body ◽  
Research Center ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Aging is a natural process in human that can be characterized by the appearance of black spot on the skin due to hyperpigmentation. Aging may occur due to an excessive amount of free radicals in the body. Antioxidants possess ability to capture free radicals and inhibit tyrosinase which induces skin aging. Aloe vera has been used in traditional medicine because it contains several bioactive compounds that act as antioxidant and prevent aging process. This study aims to determine phytochemical content, antioxidant activity and tyrosinase inhibition activity of Aloe vera rind (AVRE) and gel (AVGE) extract. This research was carried out at the laboratorium of Aretha Medika Utama-Biomolecular and Biomedical Research Center in Bandung city in September–November 2018. Phytochemical assay was determined using modified Farnsworth method. Antioxidant assay was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and antiaging assay was obtained using tyrosinase inhibition assay. AVRE contains flavonoid, phenol, steroid, and alkaloid. Meanwhile, AVGE contains steroid and alkaloid. IC50 DPPH scavenging activity of AVRE was 113.18 µg/mL followed by AVGE was 291.96 µg/mL. IC50 tyrosinase inhibition activity of AVRE was 65.04 µg/mL followed by AVGE was 111.89 µg/mL. AVRE had more active DPPH scavenging activity and tyrosinase inhibition activity than AVGE. AKTIVITAS ANTIOKSIDAN DAN ANTITIROSINASE KULIT DAN DAGING LIDAH BUAYA (ALOE VERA)Penuaan merupakan proses alamiah pada manusia. Penuaan dapat terjadi akibat kadar radikal bebas yang tinggi di dalam tubuh. Antioksidan memiliki kemampuan memerangkap radikal bebas dan menghambat kerja enzim yang berperan dalam proses penuaan. Lidah buaya (Aloe vera) telah digunakan dalam pengobatan tradisional karena diketahui mengandung senyawa bioaktif yang bermanfaat dalam menangkal radikal bebas dan menghambat penuaan. Penelitian ini bertujuan mengetahui kandungan fitokimia dalam ekstrak etanol kulit lidah buaya (EKLB) dan ekstrak etanol daging lidah buaya (EDLB), mengetahui aktivitas antioksidan, pemerangkapan 2,2-difenil-1-pikrilhidrazil (DPPH), serta aktivitas antitirosinase EKLB dan EDLB. Penelitian ini dilakukan pada September–November 2018 di laboratorium Aretha Medika Utama Biomolecular and Biomedical Research Center (BBRC). Analisis fitokimia dilakukan menggunakan metode Farnsworth yang dimodifikasi. Aktivitas antioksidan dianalisis menggunakan metode pemerangkapan DPPH, sementara uji antipenuaan dilakukan menggunakan metode uji aktivitas antitirosinase. Hasil uji fitokimia menunjukkan EKLB mengandung flavonoid, fenol, steroid, dan alkaloid; sedangkan EDLB mengandung senyawa steroid dan alkaloid. EKLB dan EDLB memiliki aktivitas pemerangkapan DPPH dengan nilai IC50 secara berurutan 113,18 µg/mL dan 291,96 µg/mL. Penghambatan tirosinase EKLB dan EDLB dengan nilai IC50 secara berurutan 65,04 µg/mL dan 111,89 µg/mL. EKLB memiliki aktivitas antioksidan dan penghambatan tirosinase yang lebih baik dibanding dengan EDLB.

scholarly journals Evaluation of Thermal Effects on the Bioactivity of Curcumin Microencapsulated with Porous Starch-Based Wall Material Using Spray Drying

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 172 ◽  
Author(s):  
Chenwei Huang ◽  
Shengwen Wang ◽  
Huaiwen Yang
Keyword(s):  
Free Radical ◽  
Heating Temperature ◽  
Radical Scavenging ◽  
Water Bath ◽  
Wall Material ◽  
Scavenging Activity ◽  
Significant Difference ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Water Bath Heating

Curcumin was microencapsulated by porous starch using a spray dryer with a particle size between 1.5 and 2.0 µm and subjected to water bath (40–100 °C) and oven heating (150–200 °C) in comparison to non-encapsulated samples. The minimum possible encapsulation rate ranged from 26.75 to 52.23%. A reasonable thermal stability was observed after water bath heating with regard to 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) scavenging activity. On the other hand, the increase in oven heating temperature caused significant alterations compared with the control samples (p < 0.05). The encapsulated particles subjected to oven heating at 170 °C demonstrated serious collapse. The DPPH scavenging activity of non-encapsulated curcumin was significantly reduced (p < 0.05) from 48.94% ± 3.72% (control, 0 °C) to 40.42% ± 2.23% (oven heating, 160 °C); however, remained stable for the encapsulated samples (51.18% ± 4.86%–50.02% ± 1.79%) without significant difference (p < 0.05). The ABTS scavenging activity was promoted as a function of the oven heating temperature. Both DPPH and ABTS free radical scavenging activities remained stable after water bath. Nevertheless, the color of microencapsulated curcumin was better preserved in comparison to the controls.

scholarly journals A Study on Organic Tomatoes: Effect of a Biostimulator on Phytochemical and Antioxidant Activities

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Varinder Sidhu ◽  
Dilip Nandwani ◽  
Li Wang ◽  
Ying Wu
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Treatment Groups ◽  
Significant Difference ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The objective of the present study is to investigate nutritional and antioxidant activity of four types of organic tomato cultivars. The differences in tomato quality are also tested between groups with or without treatment using an organic biostimulator, Stimplex. Total phenolic compounds (TPC), lycopene,β-carotene, DPPH free radical scavenging activity, reducing power, and color parameters were investigated in the current study. The results showed that there was no significant difference in TPC among cultivars regardless of Stimplex treatment. Higher lycopene andβ-carotene were obtained in Stimplex treated tomatoes. Lycopene andβ-carotene contents were significantly different among cultivars (P<0.05). DPPH scavenging activity in controlled group was significantly higher than that in the Stimplex treated tomatoes (P<0.05). No significant difference in reducing power was detected among cultivars treatment groups. The study showed that the darker the tomato color, the higher the lycopene andβ-carotene contents and the stronger the reducing power.

scholarly journals Lactic Acid Fermented Green Tea with Levilactobacillus brevis Capable of Producing γ-Aminobutyric Acid

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 110
Author(s):  
Young Hun Jin ◽  
Jong Hyoung Hong ◽  
Jun-Hee Lee ◽  
Hyeock Yoon ◽  
Alixander Mattay Pawluk ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Green Tea ◽  
Bioactive Compounds ◽  
Growth Potential ◽  
Rrna Gene ◽  
Scavenging Activity ◽  
Gaba Production ◽  
Green Tea Leaves ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The antioxidative activity and bioactive compounds content of lactic acid fermented green tea (LFG) fermented with an outstanding GABA-producing strain under optimised fermentation conditions were evaluated. Levilactobacillus strain GTL 79 was isolated from green tea leaves and selected based on acid production, growth potential, catechin resistance, and GABA production to be applied to LFG. Through 16S rRNA gene sequence analysis, the strain was identified as Levilactobacillus brevis. The optimised conditions were defined as fermentation at 37 °C with supplementation of 1% fermentation alcohol, 6% glucose, and 1% MSG and was determined to be most effective in increasing the lactic acid, acetic acid, and GABA content in LFG by 522.20%, 238.72% and 232.52% (or 247.58%), respectively. Initial DPPH scavenging activity of LFG fermented under the optimised conditions was 88.96% and rose to 94.38% by day 5. Polyphenols may contribute to the initial DPPH scavenging activity, while GABA and other bioactive compounds may contribute to the activity thereafter. Consequently, as GABA and other bioactive compounds found in green tea have been reported to have health benefits, future studies may prove that optimally fermented LFG by L. brevis GTL 79 could be useful in the food and health industries.

scholarly journals Determination of Antioxidant Activity of Leave Extracts of Albizia chevalieri Using Free Radical Scavenging Activity Assay

2019 ◽  
pp. 1-6
Author(s):  
Alhassan M. Garba ◽  
Habiba R. Isa ◽  
Sadiq Abubakar ◽  
Saudat Ja’afar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Activity Assay ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Dried and powdered leaves of Albizia chevalieri were extracted using ethanol. The extract was fractionated to give methanol, chloroform and pet-ether. The four extracts obtained; ethanol, chloroform, methanol and pet-ether were evaluated for antioxidant activity using 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) free radical scavenging activity assay. The results of the DPPH scavenging activity indicated a concentration-dependent antioxidant activity. The DPPH scavenging activity of the ethanol, chloroform and methanol extracts were found to be promising. There is no significant difference in the antioxidant activity between the ethanol, chloroform and methanol extracts with that of standard Ascorbic acid at 10, 25, 250 and 500 μg/ml concentrations. This showed that the ethanol, chloroform and methanol leave extracts of the plant has the potency of scavenging free radicals in vitro and may provide leads in the ongoing search for natural antioxidants from Nigerian medicinal plants to be used in treating diseases related to free radical reactions.

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