scholarly journals Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound

2019 ◽  
Vol 30 (4) ◽  
pp. 240
Author(s):  
Dela Vrianty ◽  
Rismawati Laila Qodariah ◽  
Wahyu Widowati ◽  
Ade Putra Fratama Sinaga ◽  
Dewi Fibrina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Enzyme Inhibition ◽  
Final Concentration ◽  
Ananas Comosus ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Tyrosinase Enzyme ◽  
Dpph Scavenging

Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.

scholarly journals PHYTOCHEMICAL IDENTIFICATION AND ANTIOXIDANT ACTIVITY OF PASSIFLORA FOETIDA FRUITS AND LEAVES EXTRACTS: A COMPARATIVE STUDY

2020 ◽  
pp. 55-58 ◽  
Author(s):  
YOHANES TANDORO ◽  
PAINI SRI WIDYAWATI ◽  
TARSISIUS DWI WIBAWA BUDIANTA ◽  
GRACE SUMARGO
Keyword(s):  
Antioxidant Activity ◽  
Cardiac Glycosides ◽  
Reducing Power ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Scavenging Activity ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Passiflora Foetida ◽  
Dpph Scavenging

Objective: The objective of this study was to compare the phytochemical composition and antioxidant activity of Passiflora foetida fruits and leaves extract. Methods: The parameters observed in this study were phytochemical compounds including alkaloid, flavonoid, phenolic, sterol, triterpenoid, saponin, tannin, and cardiac glycoside, total phenolic content Folin Ciocalteu method is based on reduction of Folin Ciocalteu reagent in alkaline medium; the metal complex produced measured at λmax: 760 nm; total flavonoids content with AlCl3 Colorimetric method based on complex formation of AlCl3 and flavonoid content in alkaline medium, the AlCl3-flavonoid complex produced measured at λmax: 510 nm; free radical DPPH scavenging activity; and ferric reducing power based on reduction of Fe3+ion into Fe2+ion that reacted with FeCl3 to form a ferric-ferrous complex that measured at λmax: 700 nm. Results: Passiflora leaves extract has phytochemical compound such as alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides, total phenol was 22.92±0.18 mg GAE/g sample dry base, total flavonoid was 7.01±0.10 mg CE/g sample dry base, DPPH scavenging activity was 2.77±0.02 mg GAE/g sample dry base and ferric reducing power was 3.20±0.04 mg GAE/g sample dry base meanwhile Passiflora fruits extract had phytochemical compounds such as alkaloid, phenolic, flavonoids, cardiac glycosides, total phenol was 6.53±1.02 mg GAE/g sample dry base, total flavonoids were 1.56±0.27 mg CE/g sample dry base, DPPH free radical scavenging activity was 1.00±0.15 mg GAE/g sample dry base, and ferric reducing power was 1.12±0.17 mg GAE/g sample dry base. Conclusion: Passiflora leaves extract has higher total phenol, total flavonoid and antioxidant activity measured by DPPH scavenging activity and ferric reducing power value compared with Passiflora fruits extract.

scholarly journals IN VITRO ANTIOXIDANT CAPACITIES AND ANTIDIABETIC PROPERTIES OF PLUCHEA LEAVES AND GREEN TEA MIXTURES AT VARIOUS PROPORTIONS

2017 ◽  
Vol 9 (8) ◽  
pp. 203 ◽  
Author(s):  
Paini Sri Widyawati ◽  
Yesiana D. W. Werdani ◽  
Christine Setiokusumo
Keyword(s):  
Free Radical ◽  
Green Tea ◽  
Reducing Power ◽  
Alpha Amylase ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Graph Pattern ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Objective: This study was done to determine the effect of pluchea leaves and green tea mixtures at various proportions to antioxidant and antidiabetic properties.Methods: The research used a single factor randomized block design. Research factors were pluchea and green tea mixture proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w), respectively. Each of factors was repeated five times. The parameters observed in this study were phytochemical compounds, total phenols, total flavonoids, free radical DPPH scavenging activity, iron ion reducing power, alpha amylase and alpha glycosidase inhibition activities. The data were statistically α = 5%, if the data analysis showed a significant analyzed using analysis of variance (ANOVA) at effect then it was continued with duncan's multiple range test (DMRT).Results: The phytochemical compounds containing in pluchea leaves and green tea mixtures were alkaloids, flavonoids, phenolics, saponins, tannins, triterpenoids, sterols, and cardiac glycosides. The qualitative assay of phytochemical compounds from aqueous extract had a different pattern with total phenolic content (TPC) and total flavonoid content (TFC), especially at 75:25; 50:50 and 25:75 % (w/w) from pluchea leaves and green tea mixture proportions. The 50:50% (w/w) proportions of samples had the highest TPC and TFC, it was proposed that there was an interaction between bioactive compounds of pluchea leaves and green tea. TPC and TFC were correlated with free radical DPPH scavenging activity, iron reducing power and alpha glycosidase inhibition activity, but the alpha amylase inhibition activity had the different graph pattern with the TPC and TFC.Conclusion: The antioxidant capacity of pluchea leaves and green tea mixtures at various proportions had the graph pattern similar to the alpha glycosidase inhibitory activity and different with the alpha amylase inhibitory activity.

scholarly journals Phytochemical Evaluation of Hibiscus Sabdariffa Powder, Jam and Yoghurt

2020 ◽  
Author(s):  
Karlin Hamwenye ◽  
Helena Shindaadhi ◽  
Elizabeth Sivhute ◽  
Penny Hiwilepo-van Hal ◽  
Chinemba Samundengu
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic ◽  
Hibiscus Sabdariffa ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Phytochemical Content ◽  
Phytochemical Compounds ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phytochemical Contents

Abstract BackgroundHibiscus sabdariffa is popularly known as food and herbal drink with numerous health benefits. The phytochemical compounds present in Hibiscus sabdariffa calyces are important in developing nutraceutical foods. In this study hibiscus jam and yoghurt were produced from dried hibiscus calyces’ powder.MethodsThe phytochemical content and antioxidant activity of these products were then analysed in terms of Total phenolic content (TPC), Total flavonoid content (TFC), Condensed tannins (CT) and DPPH Scavenging activity.ResultsThe results showed the presence of all phytochemical compounds (TPC, TFC, CT) and antioxidant activity in all Hibiscus sabdariffa products. The hibiscus calyces powder showed the highest phytochemical contents of 35.24 mg GAE. g -1 , 0.91 mg QE. g -1 , 2.85 mg CAE. g -1 and 48.2 % inhibition for TPC, TFC, CT and DPPH Scavenging activity, respectively. Hibiscus jam and Hibiscus yoghurt had phytochemical contents of 6.44 and 4.81 mg GAE. g -1 , 0.19 & 0.24 mg QE. g -1 , 1.40 & 0.66 mg CAE. g -1 and 26.2 & 39.3 % inhibition for TPC, TFC, TC and DPPH Scavenging activity, respectively.ConclusionsThe results of the current study showed that there is potential in using Hibiscus sabdariffa to develop functional foods.

scholarly journals Sunflower (Helianthus annuus L.) Plants at Various Growth Stages Subjected to Extraction—Comparison of the Antioxidant Activity and Phenolic Profile

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 535 ◽  
Author(s):  
Francesco Gai ◽  
Magdalena Karamać ◽  
Michał A. Janiak ◽  
Ryszard Amarowicz ◽  
Pier Giorgio Peiretti
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Growth Stages ◽  
Scavenging Activity ◽  
Flowering Stage ◽  
Phenolic Profile ◽  
Aerial Parts ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The aim of this study was to evaluate the differences in the antioxidant activity and phenolic profile of sunflower (Helianthus annuus L.) extracts obtained from the aerial parts of plants harvested at five growth stages. In vitro assays were used to determine the antioxidant activity, i.e., ABTS•+ and DPPH• scavenging activity, the ferric-reducing antioxidant power (FRAP) and the ability to inhibit β-carotene–linoleic acid emulsion oxidation. Phenolic compounds, such as mono- and dicaffeoylquinic acid isomers and caffeic acid hexose, were identified using the LC–TOF–MS/MS technique. The predominant compound during the growth cycle of the plant was 3,5-di-O-caffeoylquinic acid, whose content was the highest at the mid-flowering stage. The total phenolic content was also the highest in sunflowers at the mid-flowering stage. The main phenolic compound contents were closely correlated with ABTS•+ and DPPH• scavenging activity and FRAP. No significant correlation was found between the total phenolic content and the antioxidant activity in the emulsion system. The highest antiradical activity and FRAP were generally determined in older plants (mid-flowering and late flowering stages). In conclusion, the aerial parts of sunflowers, in particular those harvested at the mid-flowering stage, are a good plant material from which to obtain phenolic compound extracts, albeit mainly of one class (esters of caffeic acid and quinic acid), with high antioxidant activity.

scholarly journals Optimization of the Extraction and Stability of Antioxidative Peptides from Mackerel (Pneumatophorus japonicus) Protein

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Xueqin Wang ◽  
Huahua Yu ◽  
Ronge Xing ◽  
Xiaolin Chen ◽  
Song Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Protein Hydrolysate ◽  
Scientific Basis ◽  
Enzyme Concentration ◽  
Scavenging Activity ◽  
Preparation Conditions ◽  
Low Concentrations ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
The Stability

This study optimizes the preparation conditions for mackerel protein hydrolysate (MPH) by response surface methodology (RSM) and investigates the stability of the antioxidant activity of MPHs (<2.5 kDa). The optimal conditions were as follows: enzyme concentration of 1726.85 U/g, pH of 7.00, temperature of 39.55°C, time of 5.5 h, and water/material ratio of 25 : 1, and the maximum DPPH scavenging activity was 79.14%. The MPHs indicated significant cellular antioxidant activity at low concentrations. Furthermore, the temperature and freeze-thaw cycles had little effect on the antioxidative stability while pH had significant effect on the antioxidative stability. In addition, the MPHs were sensitive to the metal ions, such as Fe2+, Fe3+, Zn2+, and Cu2+. Notably, when the concentrations of Fe2+and Fe3+were 5 mM, the DPPH scavenging activities were only 1.1% and 0.6%, respectively; furthermore, Cu2+at a 5 mM concentration could completely inhibit the DPPH scavenging activity of MPHs. In contrast, K+and Mg2+had no notable effect on the antioxidant activity of MPHs. These results may provide a scientific basis for the processing and application of MPHs.

Antioxidant profiling of Hippophae salicifolia growing in sacred forests of Sikkim, India

2011 ◽  
Vol 38 (9) ◽  
pp. 697 ◽  
Author(s):  
Arvind K. Goyal ◽  
Bharat C. Basistha ◽  
Arnab Sen ◽  
Sushil K. Middha
Keyword(s):  
Antioxidant Activity ◽  
Sea Buckthorn ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Methanol Extracts ◽  
Hippophae Salicifolia ◽  
Total Antioxidant ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging ◽  
Phenolic And Flavonoid

Hippophae commonly known as sea-buckthorn, is a versatile plant with multipurpose uses including the control of soil erosion, biological nitrogen fixation and medicinal properties. In India, two species of sea-buckthorn; namely, Hippophae salicifolia D. Don and Hippophae rhamnoides L., are very common. H. salicifolia has a shrub-to-tree habit and is restricted to the Himalayan region, whereas H. rhamnoides is bushy, growing at higher altitude in India, and widely distributed in Europe and Asia. In this study, aqueous, methanol and acetone extracts of various parts of female and male plants of H. salicifolia were evaluated for total antioxidant activity, phenolic and flavonoid contents, and correlations were made. The total phenolic and flavonoid content in all the samples ranged from 99 ± 32 to 1459 ± 53 mg gallic acid equivalent g–1, 135 ± 12 to 707 ± 62 mg quercetin equivalent g–1 of dry extract, respectively. The highest free radical (DPPH) scavenging activity was found in methanol extracts of female bark and male leaf. Methanol extracts of male bark and male leaf showed maximum H2O2 activity at a concentration of 1 mg mL–1. Antioxidant activity (FRAP method) ranged from 0.021 to 1.041 at concentrations ranging from 20 to 200 µg mL–1 with a reducing capacity of extracts as water > methanol > acetone. Strong correlation (P < 0.05) was observed between DPPH scavenging activity and total phenolic content from all the extracts under study.

scholarly journals Chemical Constituents Antioxidant and Anticholinesterasic Activity ofTabernaemontana catharinensis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Carla Nicola ◽  
Mirian Salvador ◽  
Adriana Escalona Gower ◽  
Sidnei Moura ◽  
Sergio Echeverrigaray
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Neurodegenerative Diseases ◽  
Chemical Constituents ◽  
Ethanolic Extract ◽  
Alkaloid Content ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

The present work aimed to analyze the alkaloid content of the ethanolic extract ofTabernaemontana catharinensis(Apocynaceae family) and its fractions as well as to evaluate their antioxidant and anticholinesterasic activities. The analyses of the ethanolic extract ofT. catharinensisby mass spectrometry allowed identifying the presence of the alkaloids 16-epi-affinine, coronaridine-hydroxyindolenine, voachalotine, voacristine-hydroxyindolenine, and 12-methoxy-n-methyl-voachalotine, as well as an alkaloid withm/z385.21 whose spectrum suggests a derivative of voacristine or voacangine. The extract and its alkaloid rich fractions showed antioxidant activity, especially those that contain the alkaloidm/z385.21 or 16-epi-affinine with DPPH scavenging activity (IC50) between 37.18 and 74.69 μg/mL. Moreover, the extract and its fractions exhibited anticholinesterasic activity, particularly the fractions characterized by the presence of 12-methoxy-n-methyl-voachalotine, with IC50= 2.1 to 2.5 μg/mL. Fractions with 16-epi-affinine combined good antioxidant (IC50= 65.59 to 74.69 μg/mL) and anticholinesterasic (IC50= 7.7 to 8.3 μg/mL) activities, representing an option for further studies aimed at treating neurodegenerative diseases.

Preparation and characterization of phloretin by complexation with cyclodextrins

2020 ◽  
Vol 44 (14) ◽  
pp. 5218-5223
Author(s):  
Xiaona Hu ◽  
Ziruo Zhou ◽  
Lijun Han ◽  
Shujing Li ◽  
Wei Zhou
Keyword(s):  
Antioxidant Activity ◽  
Inclusion Complexes ◽  
Scavenging Activity ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

RP and DPPH scavenging activity tests revealed that the antioxidant activity of phloretin improved after the formation of inclusion complexes.

scholarly journals In vitro studies on antioxidant, ? – amylase and ? – glucosidase inhibitory activities of ethanol extracts of Syzygium cumini seeds

Biomedicine ◽  
2020 ◽  
Vol 39 (2) ◽  
pp. 258-262
Author(s):  
M. Narmatha ◽  
S. Maneemegalai
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Extract ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Syzygium Cumini ◽  
Total Alkaloids ◽  
Dpph Scavenging Activity ◽  
Inhibitory Activities ◽  
Dpph Scavenging

Introduction and Aim: Diabetes mellitus is a metabolic disorder leads to many secondary complications. The drugs used for treatment causes serious side effects. Syzygium cumini is used in traditional medicine for treating many diseases. The aim of the present study is to estimate phytochemical contents, antioxidant activity, ? – amylase and ? – glucosidase inhibitory activities of ethanol extract of seeds of S. cumini by in vitro methodology. Materials and Methods: The powdered seeds were extracted with ethanol. Quantitative analysis of Total alkaloids, Total phenols, Total flavonoids, Total tannins, Total saponins and Total steroids were carried out. DPPH scavenging activity, ? – amylase and ? – glucosidase inhibitory activities were measured with varying concentration of extract. Results: Total alkaloids content was observed to be higher followed by Total phenol content. Total saponin was found to be present in lesser amount among the tested phytochemicals. 500 µg/ml and above concentrations of seed extract possess above 90% DPPH scavenging activity; 1000 µg/ml concentration of the extract exhibited 43.20% and 19.80% inhibition activity on ? – amylase and ? – glucosidase enzymes. Conclusion: The above results indicate a higher antioxidant activity and appreciable inhibitory activities of enzymes responsible for elevated circulation of glucose. These activities are due to the presence of phytochemicals present in the seeds extract of S. cumini and can be utilized for the management of Diabetes.  

scholarly journals Antioxidant and Anti-tyrosinase Activities of Aloe vera Rind and Gel Extracts

2019 ◽  
Vol 7 (3) ◽  
Author(s):  
Sonya Bonifacia Mahadi ◽  
Rr. Anisa Siwianti Handayani ◽  
Wahyu Widowati ◽  
Wilsen Wilsen ◽  
Yunita Dewani ◽  
...  
Keyword(s):  
Free Radicals ◽  
Biomedical Research ◽  
Aloe Vera ◽  
The Body ◽  
Research Center ◽  
Scavenging Activity ◽  
Inhibition Activity ◽  
Tyrosinase Inhibition ◽  
Dpph Scavenging Activity ◽  
Dpph Scavenging

Aging is a natural process in human that can be characterized by the appearance of black spot on the skin due to hyperpigmentation. Aging may occur due to an excessive amount of free radicals in the body. Antioxidants possess ability to capture free radicals and inhibit tyrosinase which induces skin aging. Aloe vera has been used in traditional medicine because it contains several bioactive compounds that act as antioxidant and prevent aging process. This study aims to determine phytochemical content, antioxidant activity and tyrosinase inhibition activity of Aloe vera rind (AVRE) and gel (AVGE) extract. This research was carried out at the laboratorium of Aretha Medika Utama-Biomolecular and Biomedical Research Center in Bandung city in September–November 2018. Phytochemical assay was determined using modified Farnsworth method. Antioxidant assay was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and antiaging assay was obtained using tyrosinase inhibition assay. AVRE contains flavonoid, phenol, steroid, and alkaloid. Meanwhile, AVGE contains steroid and alkaloid. IC50 DPPH scavenging activity of AVRE was 113.18 µg/mL followed by AVGE was 291.96 µg/mL. IC50 tyrosinase inhibition activity of AVRE was 65.04 µg/mL followed by AVGE was 111.89 µg/mL. AVRE had more active DPPH scavenging activity and tyrosinase inhibition activity than AVGE. AKTIVITAS ANTIOKSIDAN DAN ANTITIROSINASE KULIT DAN DAGING LIDAH BUAYA (ALOE VERA)Penuaan merupakan proses alamiah pada manusia. Penuaan dapat terjadi akibat kadar radikal bebas yang tinggi di dalam tubuh. Antioksidan memiliki kemampuan memerangkap radikal bebas dan menghambat kerja enzim yang berperan dalam proses penuaan. Lidah buaya (Aloe vera) telah digunakan dalam pengobatan tradisional karena diketahui mengandung senyawa bioaktif yang bermanfaat dalam menangkal radikal bebas dan menghambat penuaan. Penelitian ini bertujuan mengetahui kandungan fitokimia dalam ekstrak etanol kulit lidah buaya (EKLB) dan ekstrak etanol daging lidah buaya (EDLB), mengetahui aktivitas antioksidan, pemerangkapan 2,2-difenil-1-pikrilhidrazil (DPPH), serta aktivitas antitirosinase EKLB dan EDLB. Penelitian ini dilakukan pada September–November 2018 di laboratorium Aretha Medika Utama Biomolecular and Biomedical Research Center (BBRC). Analisis fitokimia dilakukan menggunakan metode Farnsworth yang dimodifikasi. Aktivitas antioksidan dianalisis menggunakan metode pemerangkapan DPPH, sementara uji antipenuaan dilakukan menggunakan metode uji aktivitas antitirosinase. Hasil uji fitokimia menunjukkan EKLB mengandung flavonoid, fenol, steroid, dan alkaloid; sedangkan EDLB mengandung senyawa steroid dan alkaloid. EKLB dan EDLB memiliki aktivitas pemerangkapan DPPH dengan nilai IC50 secara berurutan 113,18 µg/mL dan 291,96 µg/mL. Penghambatan tirosinase EKLB dan EDLB dengan nilai IC50 secara berurutan 65,04 µg/mL dan 111,89 µg/mL. EKLB memiliki aktivitas antioksidan dan penghambatan tirosinase yang lebih baik dibanding dengan EDLB.

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