Study of confounding factors influence on lung cancer diagnostics effectiveness using gas chromatography–mass spectrometry analysis of exhaled breath

2021 ◽  
Author(s):  
Elina Gashimova ◽  
Anna Osipova ◽  
Azamat Temerdashev ◽  
Vladimir Porkhanov ◽  
Igor Polyakov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Lung Cancer ◽  
Gas Chromatography ◽  
Cancer Patients ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diagnostic Model ◽  
Exhaled Breath ◽  
Lung Cancer Patients ◽  
Chromatography Mass Spectrometry

Aim: The purpose of this study was to estimate volatile organic compounds (VOCs) ability to distinguish exhaled breath samples of lung cancer patients and healthy volunteers and to assess the effect of smoking status and gender on parameters. Patients & methods: Exhaled breath samples of 40 lung cancer patients and 40 healthy individuals were analyzed using gas chromatography–mass spectrometry. Influence of other factors on the exhaled breath VOCs profile was investigated. Results: Some parameters correlating with the disease status were affected by other factors. Excluding these parameters allows creating a logistic regression diagnostic model with 83% sensitivity and 81% specificity. Conclusion: Influence of other factors on the exhaled breath VOCs profile has to be taken into account to avoid misleading results.

scholarly journals Comprehensive Two-Dimensional Gas Chromatography–Mass Spectrometry Analysis of Exhaled Breath Compounds after Whole Grain Diets

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2667
Author(s):  
Kaisa Raninen ◽  
Ringa Nenonen ◽  
Elina Järvelä-Reijonen ◽  
Kaisa Poutanen ◽  
Hannu Mykkänen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Metabolic Effects ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Wheat Bread ◽  
Exhaled Breath ◽  
Two Dimensional ◽  
Whole Grain ◽  
Chromatography Mass Spectrometry

Exhaled breath is a potential noninvasive matrix to give new information about metabolic effects of diets. In this pilot study, non-targeted analysis of exhaled breath volatile organic compounds (VOCs) was made by comprehensive two-dimensional gas chromatography–mass spectrometry (GCxGC-MS) to explore compounds relating to whole grain (WG) diets. Nine healthy subjects participated in the dietary intervention with parallel crossover design, consisting of two high-fiber diets containing whole grain rye bread (WGR) or whole grain wheat bread (WGW) and 1-week control diets with refined wheat bread (WW) before both diet periods. Large interindividual differences were detected in the VOC composition. About 260 VOCs were detected from exhaled breath samples, in which 40 of the compounds were present in more than half of the samples. Various derivatives of benzoic acid and phenolic compounds, as well as some furanones existed in exhaled breath samples only after the WG diets, making them interesting compounds to study further.

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