Bacteriophages carrying Shiga toxin genes: genomic variations, detection and potential treatment of pathogenic bacteria

2011 ◽  
Vol 6 (8) ◽  
pp. 909-924 ◽  
Author(s):  
Joanna M Łoś ◽  
Marcin Łoś ◽  
Grzegorz Węgrzyn
Keyword(s):  
Shiga Toxin ◽  
Pathogenic Bacteria ◽  
Potential Treatment ◽  
Genomic Variations ◽  
Toxin Genes ◽  
Shiga Toxin Genes

scholarly journals Shiga toxin genes (stx) in Norwegian sheep herds

2001 ◽  
Vol 127 (01) ◽  
Author(s):  
A. M. URDAHL ◽  
O. ALVSEIKE ◽  
E. SKJERVE ◽  
Y. WASTESON
Keyword(s):  
Shiga Toxin ◽  
Toxin Genes ◽  
Shiga Toxin Genes

scholarly journals Shiga Toxins, and the Genes Encoding Them, in Fecal Samples from Native Idaho Ungulates

2008 ◽  
Vol 75 (3) ◽  
pp. 862-865 ◽  
Author(s):  
Jeremy J. Gilbreath ◽  
Malcolm S. Shields ◽  
Rebekah L. Smith ◽  
Larry D. Farrell ◽  
Peter P. Sheridan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Wild Ungulates ◽  
Fecal Samples ◽  
Shiga Toxins ◽  
Toxin Genes ◽  
Genes Encoding ◽  
Shiga Toxin Genes

ABSTRACT Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle (∼19%).

Presence and Antimicrobial Resistance of Escherichia coli in Ready-to-Eat Foods in Shaanxi, China

2017 ◽  
Vol 80 (3) ◽  
pp. 420-424 ◽  
Author(s):  
Allah Bux Baloch ◽  
Hua Yang ◽  
Yuqing Feng ◽  
Meili Xi ◽  
Qian Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Shaanxi Province ◽  
Future Research ◽  
E Coli ◽  
Class 1 Integrons ◽  
Toxin Genes ◽  
Class 1 ◽  
Shiga Toxin Genes ◽  
Cooked Meat

ABSTRACT The aim of this study was to determine the presence and characteristics of Escherichia coli in ready-to-eat (RTE) foods. A total of 300 RTE foods samples were collected in Shaanxi Province, People's Republic of China: 50 samples of cooked meat, 165 samples of vegetable salad, 50 samples of cold noodles, and 35 samples of salted boiled peanuts. All samples were collected during summer (in July to October) 2011 and 2012 and surveyed for the presence of E. coli. E. coli isolates recovered were classified by phylogenetic typing using a PCR assay. The presence of Shiga toxin genes 1 (stx1) and 2 (stx2) was determined for these E. coli isolates by PCR, and all isolates were analyzed for antimicrobial susceptibility and the presence of class 1 integrons. Overall, 267 (89.0%) RTE food samples were positive for E. coli: 49 cold noodle, 46 cooked meat, 150 salad vegetable, and 22 salted boiled peanut samples. Of the 267 E. coli isolates, 73.0% belong to phylogenetic group A, 12.4% to group B1, 6.4% to group B2, and 8.2% to group D. All isolates were negative for both Shiga toxin genes. Among the isolates, 74.2% were resistant to at least one antimicrobial agent, and 17.6% were resistant to three or more antimicrobial agents. Resistance to ampicillin (75.6% of isolates) and tetracycline (73.1% of isolates) was most frequently detected; 26.2% of E. coli isolates and 68.8% of multidrug-resistant E. coli isolates were positive for class 1 integrons. All isolates were sensitive to amikacin. Our findings indicate that RTE foods in Shaanxi were commonly contaminated with antibiotic-resistant E. coli, which may pose a risk for consumer health and for transmission of antibiotic resistance. Future research is warranted to track the contamination sources and develop appropriate steps that should be taken by government, industry, and retailers to reduce microbial contamination in RTE foods.

scholarly journals Isolation of Shiga toxin-producing Escherichia coli harboring variant Shiga toxin genes from seafood

2018 ◽  
Vol 11 (3) ◽  
pp. 379-385 ◽  
Author(s):  
Sreepriya Prakasan ◽  
Parmanand Prabhakar ◽  
Manjusha Lekshmi ◽  
Binaya Bhusan Nayak ◽  
Sanath Kumar
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Toxin Genes ◽  
Shiga Toxin Genes

scholarly journals High-resolution melting real-time PCR assays for detection of Escherichia coli O26 and O111 strains possessing Shiga toxin genes

LWT ◽  
2020 ◽  
Vol 131 ◽  
pp. 109785
Author(s):  
Prashant Singh ◽  
Gabriel Cubillos ◽  
Gabrielle Kirshteyn ◽  
Joseph M. Bosilevac
Keyword(s):  
Escherichia Coli ◽  
High Resolution ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
High Resolution Melting ◽  
Toxin Genes ◽  
Pcr Assays ◽  
Shiga Toxin Genes
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