Effects of Different Ethanolic Extract of Azadirachata indica on Blood Glucose Level in Alloxan Induced Diabetic Swiss Albino Mice

AbstractThe current study aimed to evaluate the in vivo hypoglycemic potential of Myristica fragrans seed extract co-administered with glimepiride in Swiss albino mice. Computational tools were used to further verify the in vivo findings and to help compare this combination to the glimepiride-pioglitazone combination in terms of the binding affinity of the ligands to their respective target protein receptors and the relative stability of the drug-protein complexes. The effect of the combined therapy was observed both in alloxan- and glucose-induced hyperglycemic Swiss albino mice. The mean fasting blood glucose level of the test groups was measured and statistically evaluated using Student’s t test. The combined therapy significantly reduced the blood glucose level in a time-dependent manner compared to glimepiride alone. The binding affinity of glimepiride was found to be −7.6 kcal/mol with sulfonylurea receptor 1 in molecular docking. Conversely, macelignan-peroxisome proliferator-activated receptor (PPAR) α and macelignan-PPAR γ complexes were stabilized with −9.2 and −8.3 kcal/mol, respectively. Molecular dynamic simulation revealed that macelignan-PPAR α and γ complexes were more stable than pioglitazone complexes. The combination shows promise in animal and computer models and requires further trials to provide evidence of its activity in humans.

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Effect of Curcumin on Blood Glucose Level and Some Neurobehavioral Responses in Alloxan-induced Diabetic Swiss Albino Mice

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2017/34323 ◽

2017 ◽

Vol 14 (1) ◽

pp. 1-7

Author(s):

U Garkuwa ◽

A Alhassan ◽

Y Tanko

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Swiss Albino Mice ◽

Albino Mice

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Curcuma longa supplement increases anxiety-like behavior and blood glucose level in Swiss albino mice

Neuroscience Research Notes ◽

10.31117/neuroscirn.v4i1.60 ◽

2021 ◽

Vol 4 (1) ◽

pp. 1-6

Author(s):

Usman Garkuwa ◽

Buhari Ibrahim ◽

Aisha Balanmalam ◽

Sayyadatu Muhammad ◽

Mustapha Muazu ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Curcuma Longa ◽

Fasting Blood Glucose ◽

Control Group ◽

Fasting Blood Glucose Level ◽

In Vivo Models ◽

Swiss Albino Mice ◽

Albino Mice

Curcuma longa (C. longa), also known as curcumin, is a lipophilic polyphenol substance proven to have cholesterol-lowering, anti-diabetic, anti-inflammatory, anti-oxidant, and anti-cancer properties in both in vitro and in vivo models. Most previous studies investigated the effect of C. longa on diabetic mice and therefore, there is a need to investigate the effect of C. longa on normoglycemic mice. Depression is a common consequence of anxiety that affects 21% of the world’s population. Since the prevalence of diabetes and depression is on the rise globally, it is important to search for safer and cost-effective management for these disorders. In doing so, it is therefore essential to investigate its effect in normoglycemic mice. The current study determines the effect of C. longa on blood glucose level and anxiety-like behavior in normoglycemic Swiss albino mice. A total of 20 mice were divided into four groups of five (n=5 per group). Group I (control) received distilled water 10 ml/kg, groups II, III, and IV received C. longa at 5%, 10%, and 20%, respectively, for 14 days. We found that 20% C. longa group showed a significant (p<0.05) increase in fasting blood glucose level (195.84±14.46 mg/dl) after 14 days of administration compared with the control group (134.60±4.52 mg/dl). We also found that 20% C. longa increased the anxiety-like behavior in normoglycemic Swiss albino mice compared with the control group. However, there was no significant (p>0.05) difference in both fasting blood glucose level and anxiety-like behavior between the mice treated with 5% and 10% C. longa and the control group. This study indicates that C. longa at high concentration is unsafe for consumption by normoglycemic Swiss albino mice.

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EVALUATION OF ANTIHYPERGLYCAEMIC EFFECT OF BUTEA MONOSPERMA LEAF EXTRACT ON ADRENALIN INDUCED AND HIGH GLUCOSE FEED ANIMAL MODEL

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21501 ◽

2017 ◽

Vol 10 (12) ◽

pp. 301

Author(s):

Biswaranjan Ray ◽

Santosh Kumar Mahapatra ◽

Pradipta Kumar Behera ◽

Ashok K Panigrahi

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

High Glucose ◽

Ethanolic Extract ◽

Biologically Active ◽

Antidiabetic Activity ◽

Blood Levels ◽

Active Components ◽

Butea Monosperma

Objective: As per the ethnopharmacological information has Butea monosperma been used to treat diabetes mellitus by the tribal people of tropical and subtropical areas. However, there is no much more scientific report available about the antidiabetic property of the leaves of the plant. Hence, the study was undertaken to evaluate the antidiabetic effect of ethanolic extract of B. monosperma on blood levels of adrenaline-induced and glucose feed diabetic rabbits.Methods: The three different doses of the extracts (100, 200, and 400 mg/kg) were administered orally to an experimental animal. The animals were induced diabetics by adrenaline and high glucose diet. Blood glucose level was measured accordingly. For antidiabetic activity, photocolorimeter was used to monitoring the blood glucose level with crest kit box (GOP-POD method).Results: The extracts showed considerable dose-dependent activity. However, the dose 400 mg/kg showed considerable lower of blood glucose level. p<0.01 indicates the significance result. 8 hrs reading 182.5±3.83 for 400 mg does is most effective for reducing blood sugar.Conclusion: The study indicates that the ethanolic extract of B. monosperma leaves possesses antidiabetic properties which suggest the presence of biologically active components.

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PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2018v10i1.21250 ◽

2018 ◽

Vol 10 (1) ◽

pp. 71

Author(s):

Shibu Narayan Jana ◽

Papiya Mitra Mazumder

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Cholesterol Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Fasting Blood Glucose Level ◽

Β Cells ◽

Partial Pancreatectomy ◽

Swiss Albino Mice ◽

Diabetes Status

Objective: The present study was aimed at the development of partial pancreatectomy in a murine model for diabetes.Methods: Diabetes model was successfully developed by partial pancreatectomy method. In this study, cyclosporine was used for influenced the glycaemic status. Diabetes status was evaluated by fasting blood glucose level (FBG), lipid profile (by estimation of total cholesterol level and HDL-level), serum amylase and biochemical assay like glutathione estimation.Results: We report here the restoration of euglycaemic status in cyclosporine-induced diabetic in swiss albino mice after 30% pancreatectomy. In this study, Pancreatectomised (partial) group of animals showed a rapid elevation of glycaemic status, starting from 15th post observational d, but the level of significance decreased gradually from 15th (P<0.01) to 60th (P<0.05) d. This was probably due to nesidioblastotic activity which shifted the fasting blood glucose level towards normoglycaemic status with β-cells regeneration. Cyclosporine treated a group of mice showed normoglycaemic status throughout the whole experimental period, but the cholesterol level remained significant (P<0.001) till the end of the experimental d. Gradually decrements in glycaemia of the diabetic pancreatectomised animals demonstrate islets neogenesis occurring after the operative activity, leading to normoglycaemic condition, probably attributed to β-cells proliferation.Conclusion: The biochemical and histopathological evaluations suggest that there is the development of the diabetic model in the pancreatectomized group and diabetes status induced by pancreatectomy is curable to a certain extent due to the regeneration of β-cells.

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Antidiabetic Potential of Alcoholic and Aqueous Extracts of Ficus racemosa Linn. Bark In Normal and Alloxan Induced Diabetic Rats

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2009.010106 ◽

2009 ◽

Vol 1 (01) ◽

Cited By ~ 1

Author(s):

Nikhil K. Sachan ◽

Yatindra Kumar ◽

Seema Pushkar ◽

R. N. Thakur ◽

Sudhir S. Gangwar ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Aqueous Extract ◽

Ethanolic Extract ◽

Diabetic Rats ◽

Alcoholic Extract ◽

Glucose Lowering ◽

Ficus Racemosa ◽

Lowering Activity

The present investigation aims to examine the diabetic potential of the plant Ficus racemosa in normal and alloxan induced diabetic rats. The bark extract with water, petroleum ether and with alcohol were screened for blood glucose lowering activity and the alcoholic extract having better therapeutic potential was prepared through Soxhlet extraction for further study. Alcoholic and aqueous extract of bark of Ficus racemosa at a dose of 400 mg/Kg was given to normal and alloxan induced diabetic rats and the blood samples taken from the retero-orbital plexus vein were analyzed for blood glucose level as per standard protocol with available kits through Auto-analyzer. The comparison of blood sugar level as per model schedule showed that in normal group the ethanolic extract, at a dose of 400 mg/Kg intra-peritoneal, the blood glucose lowering 28.66 % while in aqueous extract given group it was 25.90 %. In alloxan induced diabetic rats decrease in blood glucose level in aqueous and ethanolic extract group was found to be 27.01 % and 45.03 % respectively. In conclusion, the ethanolic extract of Ficus racemosa reflected anti-diabetic potential through its glucose lowering activity in experimental animals. It supported the folklore claim of anti-diabetic activity of the plant.

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Hypoglycemic Effect of Scarlet Spiral Flag (Costus woodsonii) to Alloxan-Induced Diabetic Mice: An In Vivo Study

UI Proceedings on Health and Medicine ◽

10.7454/uiphm.v4i1.255 ◽

2020 ◽

Vol 4 (1) ◽

Author(s):

Roderic John R. Magtulis ◽

Emvie Loyd P. Itable, RN, MAN

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Blood Sugar ◽

Glucose Level ◽

Ethanolic Extract ◽

Positive Control ◽

Hypoglycemic Effect ◽

Diabetic Mice ◽

Alloxan Monohydrate

Objective: Diabetes Mellitus is a leading illness to the society and despite of a lot of researches, not a single cure has been discovered. Most of the time, people focused on fighting diabetes and medicinal plants were utilized. The purpose of this study was to investigate the hypoglycemic effect of Scarlet Spiral Flag (Costus woodsonii) and to be reviewed for preclinical trials.Methods: 20 male Swiss mice were made diabetic by inducing Alloxan monohydrate. Then separated randomly into 4 groups with different treatments administered daily for 7 days; Control, Positive Control (600 µg/kg Glibenclamide), T1 (250mg/kg Scarlet Spiral Flag ethanolic extract) and T2 (500mg/kg Scarlet Spiral Flag ethanolic extract). Their blood sugar was monitored onset after 1st treatment (6th, 12th and 24th hours) and during the week within the 1st, 4th and 8th day.Results: Both the onset and fasting blood sugar monitoring showed a significant decrease in the mean average blood glucose level of the mice.Conclusion: The ethanolic extract of Scarlet Spiral Flag (Costus woodsonii) has a potent antidiabetic effect in Alloxan-induced diabetic mice. Since it is under the same Genus of the Insulin plant, it has a common effect in terms of lowering the blood glucose level.Keywords: Acclimatization, Alloxan, Costus woodsonii, Ethanolic Extract, Oral Gavage

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Effect of different extracts of Aloe Vera gel (Aloe Barbadensis) on blood glucose level of alloxan induced hyperglycaemic mice

Bangladesh Medical Journal ◽

10.3329/bmj.v44i2.27239 ◽

2016 ◽

Vol 44 (2) ◽

pp. 61-66

Author(s):

Shammin Haque ◽

Ferdous Ara ◽

Md Jalaluddin Iqbal ◽

Sheikh Nazrul Islam

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Aloe Vera ◽

Ethanolic Extract ◽

Cost Effective ◽

Aloe Barbadensis ◽

Aloe Vera Gel ◽

Dried Extract

This study was conducted to evaluate the effect of different extracts of Aloe Vera gel in alloxan induced hyperglycaemic mice.Three different extracts of Aloe Vera gel (dried extract, ethanolic extract and fresh raw extract) were orally administered at 300 mg/kg body weight for 28 days. The fasting blood glucose level was estimated both in normal and alloxan induced hyperglycaemic mice. It was found that, when compared with the control, there was a significant reduction in blood glucose level in all three experimental groups. Ethanolic and fresh raw gel extracts were more effective than the dried extract. The extracts produced similar results when compared with gliclazide. It can be concluded that, the administration of Aloe Vera gel extract significantly decreases blood glucose level in hyperglycaemic mice. Aloe Vera gel can therefore be a natural remedy and a cost effective resource for the management of diabetes.Bangladesh Med J. 2015 May; 44 (2): 61-66

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Anti-diabetic Activity of Ethanolic Extract of Elaeocarpus serratus L. in Streptozotocin-Induced Diabetic Rats

International Journal of Pharmaceutical Sciences and Drug Research ◽

10.25004/ijpsdr.2016.080106 ◽

2016 ◽

Vol 8 (01) ◽

Author(s):

DH Geetha ◽

Indhiramuthu Jayashree ◽

M. Rajeswari

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Ethanolic Extract ◽

Diabetic Rats ◽

The Body ◽

Marker Enzymes ◽

Metabolizing Enzymes ◽

Liver Marker Enzymes

To investigate the anti-diabetic activity of Elaeocarpus serratus fruit in streptozotocin-induced (STZ) diabetic rats. The dose-dependent effects of 30days oral treatment with ethanol extracts of fruit (200 and 400 mg/kg) of E. serratus on body weight, blood glucose level, total protein, albumin, liver marker enzymes and carbohydrate metabolizing enzymes were evaluated in STZ-induced diabetic rats. Oral administration ethanolic extract of fruit of E. serratus showed significant restoration of the body weight and decrease in the blood glucose level, liver marker enzymes (ALT, AST ALP) and carbohydrate metabolizing enzymes were observed in diabetic rats. These results suggest that fruit extract of E. serratus has valuable anti-diabetic activity in STZ-induced diabetic rats which is comparable to the standard drug glibenclamide and hence might be of use in the management of diabetes.

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In vivo Assessment of Antidiabetic Potential and Mapping of Pharmacological Properties of Ethanolic Extract of Leaves of Coccinia grandis on Alloxan-induced Diabetic Rats

Asian Journal of Advanced Research and Reports ◽

10.9734/ajarr/2019/v7i230169 ◽

2019 ◽

pp. 1-9

Author(s):

Zahirul Islam ◽

Md. Rafat Tahsin ◽

Ahmad Ullah Faisal ◽

Tanzia Islam Tithi ◽

Tasnova Tasnim Nova ◽

...

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Creatinine Level ◽

Glucose Level ◽

Safety Profile ◽

Leaf Extract ◽

Physiological State ◽

Ethanolic Extract ◽

Diabetic Rats ◽

Coccinia Grandis

Diabetes is a metabolic disease and plant derived products are used to combat this deadly disease. Plant is a diverse source of numerous therapeutic compounds which can be used to ameliorate diabetes. Leaves of Coccinia grandis (L.) (Family: Cucurbitaceae) is one of the leafy vegetable that is used for this purpose traditionally. It has been used against diabetes for a very long time. Our aims were to identify the hypoglycemic effect of extract of leaves of Coccinia grandis as well as to determine its safety profile so that we could use the plant material to improve the diabetic condition. Diabetes was induced in rats by intraperitoneal injection of alloxan at a dose of 150 mg/Kg bodyweight and ethanolic extract of leaves of Coccinia grandis was fed to the rats at a dose of 750 mg/kg. We measured blood glucose level, and safety profile by measuring SGOT, SGPT and creatinine level on diabetic and non-diabetic rats before and after administration of the extract. After measuring blood glucose level, it was found that the hypoglycemic efficacy was comparable to that of metformin (p> 0.05) which was given at a dose of 500 mg/kg. Safety profile were investigated by checking SGOT, SGPT and creatinine level. It was seen that both metformin and leaf extract of Coccinia grandis improved the pathological condition induced by diabetes. Furthermore, in healthy individual rats both metformin and leaf extract of Coccinia grandis did not significantly alter the normal physiological state. It might, therefore, be inferred that the extract of leaves of Coccinia grandis could be used as a good alternative therapy to treat diabetes.

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