scholarly journals Anti-inflammatory Activity of Sulphated Polysaccharide Isolated from Ulva fasciata

2021 ◽  
Vol 9 (11) ◽  
pp. 2041-2043
Author(s):  
Shonima Govindan M
Keyword(s):  
Inflammatory Reaction ◽  
Tissue Injury ◽  
Inflammatory Activity ◽  
Leukocyte Infiltration ◽  
Oedema Formation ◽  
Sulphated Polysaccharide ◽  
Ulva Fasciata ◽  
Paw Oedema ◽  
Associated Symptoms ◽  
Anti Inflammatory

Abstract: There are various components to an inflammatory reaction that can contribute to the associated symptoms and tissue injury and these include Oedema formation, leukocyte infiltration and granuloma formation. Anti-inflammatory activity of the polysaccharide isolated from Ulva fasciata was analysed by carrageenan induced acute paw Oedema and formalin induced chronic paw Oedema. The isolated polysaccharide showed anti-inflammatory activity. Keywords: Ulva fasciata, Sulphated Polysaccharide, Paw Oedema, Anti-inflammatory Activity

scholarly journals Anti-Inflammatory Activity of Pleurotus ostreatus, a Culinary Medicinal Mushroom, in Wistar Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
W. J. A. Banukie N. Jayasuriya ◽  
Shiroma M. Handunnetti ◽  
Chandanie A. Wanigatunge ◽  
Gita H. Fernando ◽  
D. Thusitha U. Abeytunga ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Inflammatory Activity ◽  
No Production ◽  
Protective Effects ◽  
Paw Oedema ◽  
Freeze Dried ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Rat Paw Oedema

Context. Pleurotus ostreatus (P.o) is a culinary mushroom which is commonly called as “oyster mushroom” belonging to the Basidiomycetous fungi of the order Agaricales and family Pleurotaceae. Objectives. The present study investigates the anti-inflammatory potential of P.o and the underlying mechanisms of activity. Materials and Methods. Anti-inflammatory activity was evaluated using suspensions of freeze-dried and powdered (SFDP) P.o and acetone extract (AE) of P.o in normal and alloxan-induced diabetic rats using the carrageenan-induced rat paw oedema model. The mechanisms by which P.o is mediating the anti-inflammatory activity were studied using in vivo and in vitro assays. Results. At doses of 500–1000 mg/kg, the SFDP of P.o showed long-lasting activity at both early and late phases of carrageenan-induced rat paw oedema. The dose of 750 mg/kg showed the most potent inhibitory activity (92% inhibition) in healthy rats. The AE of P.o showed maximum inhibition of oedema of 87%. P.o exerted protective effects on the inflammatory pathologies in rats with diabetes. The possible mechanisms by which P.o mediates the anti-inflammatory activity were antihistamine activity (52.1%), inhibition of cell migration to the site of inflammation (45.4%), in vitro membrane stabilizing activity (52.6%), and inhibition of nitric oxide (NO) production (91.2%) (P<0.05). Dose-dependent inhibition of NO production was seen with in vitro treatment of rat peritoneal cells with AE of P.o (r = 0.95; P<0.05). Discussion and Conclusion. The promising activity of culinary mushroom P.o against inflammation suggests its potential application as a functional food during inflammatory conditions.

scholarly journals Anti-Inflammatory Activity of Hydroxytriazenes and their Vanadium Complexes

2008 ◽  
Vol 5 (s2) ◽  
pp. 1144-1148 ◽  
Author(s):  
Kalpana Singh ◽  
Peeyush Patel ◽  
A. K. Goswami
Keyword(s):  
Body Weight ◽  
Metal Complexes ◽  
Diclofenac Sodium ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Vanadium Complexes ◽  
Standard Drug ◽  
Standard Methods ◽  
Paw Oedema ◽  
Anti Inflammatory

The anti-inflammatory activity of hydroxytriazenes as well as their vanadium complexes has been studied using carrageenan induced hind paw oedema method in albino rats (Wister strain). Hydroxytriazenesviz., 3-hydroxy-1,3-diphenyltriazene(HT-1), 3-hydroxy-3-phenyl-1-(4-sulfonamido)phenyl triazene(HT-2), 3-hydroxy-3-p-chlorophenyl-1-(4-sulfonamido) phenyltriazene(HT-3), 3-hydroxy-3-m-chlorophenyl-1-(4-sulfonamido)phenyltriazene(HT-4)(HT-4) and their respective vanadium complexes C-1, C-2, C-3 and C-4 have been synthesized using standard methods, purified, characterized and used for studying their anti-inflammatory activities. The hind paw oedema was produced by subplanter injection of carrageenan and the paw volume was measured plethysmographically after 0.5, 1, 2, 3 and 5 h. The animals were givenHT-1, HT-2, HT-3andHT-4and also vanadium complexes C-1, C-2, C-3 and C-4 dissolved inDMSO(at dose 5 mg/Kg body weight). Diclofenac sodium (5 mg/kg) was used as a standard drug. The standard drug shows maximum inibition up to 1h as 81.73% which goes on increasing up to 3h (88.94%) but further reduces to 74.93% at the end of 5 h. The test compounds i.e. both ligands as well as their metal complexes show maximum percent inhibition only up to 1h. after which the efficacy reduces. Thus it can be said that both ligands as well as their vanadium complexes show very significant anti-inflammatory activity up to 1h which is comparable to standard drug.

THYMUS AND INFLAMMATION

1963 ◽  
Vol 26 (2) ◽  
pp. 209-213 ◽  
Author(s):  
R. J. GIRERD ◽  
G. DI PASQUALE
Keyword(s):  
Inflammatory Activity ◽  
Paw Oedema ◽  
Cotton Pellet ◽  
Anti Inflammatory

SUMMARY The reversal of cortisone action on formaldehyde arthritis reported by Csaba, Törő, Horváth, Ács & Mold (1962) after thymectomy in the rat did not occur using either the cotton pellet, the granuloma pouch or the acute paw-oedema procedures. Thymectomy alone caused a reduction of inflammation in both the cotton pellet and the granuloma pouch methods but had no effect on acute formaldehyde-induced paw-oedema. It is concluded that the thymus is not essential for the anti-inflammatory activity of cortisone.

Analgesic and anti-inflammatory activity of Tectona grandis Linn. stem extract

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Sapna P. Giri ◽  
Sushilkumar B. Varma
Keyword(s):  
Analgesic Activity ◽  
Radiant Heat ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Writhing Test ◽  
Paw Oedema ◽  
Stem Extract ◽  
Heat Method ◽  
Anti Inflammatory

AbstractThe objective of this work was to study the analgesic and anti-inflammatory activity ofHealthy albino rats (150–200 g) and mice (25–50 g) were arranged and divided into five groups. Group 1 received distilled water and served as control, groups 2, 3, 4 were treated with TG stem extract (100, 200, 400 mg/kg) and group 5 received standard drug. For analgesic activity, the radiant heat method and writhing test were used, and for anti-inflammatory activity, the paw oedema model and granuloma pouch method were used. Statistical analysis was done by one-way analysis of variance followed by the t-test.In the radiant heat method, there was a dose-dependent increase in reaction time in TG stem extract (100, 200, 400 mg/kg) and standard drug (buprenorphine). In the writhing test, TG stem extract (200, 400 mg/kg) and aspirin decreased the number of writhes which was significant (p<0.01, p<0.001) as compared to controls. The percentage protection was 8.88%, 15.55%, 22.22% and 35.55% in groups 2, 3, 4 and 5, respectively. In the rat paw oedema test, TG stem extract (200, 400 mg/kg) and aspirin showed 20%, 40% and 54% inhibition of oedema which was statistically significant (p<0.05, p<0.01, p<0.001), respectively, as compared to control at the end of 4 h. In the granuloma pouch method, TG stem extract (100, 200, 400 mg/kg) and aspirin showed 13.33%, 22.22%, 33.33% and 66.66% inhibition of granuloma, respectively, which was significant (p<0.05, p<0.01, p<0.001).TG stem extract possesses significant anti-inflammatory and analgesic activity.

scholarly journals Biological Evaluation and Docking Analysis of Daturaolone as Potential Cyclooxygenase Inhibitor

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Abdur Rauf ◽  
Francesco Maione ◽  
Ghias Uddin ◽  
Muslim Raza ◽  
Bina S. Siddiqui ◽  
...  
Keyword(s):  
In Silico ◽  
Biological Evaluation ◽  
Inflammatory Activity ◽  
Active Constituent ◽  
Ligand Complex ◽  
Reference Drug ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Dose Dependent

This study deals with the isolation of the active constituent(s) from a methanolic extract ofPistacia integerrimaJ. L. Stewart barks and it was also oriented to evaluate thein vivoandin silicoanti-inflammatory activity. By NMR and crystallography techniques, we have isolated a triterpenoid identified as daturaolone (compound1). This compound showedin vivoa significant and dose dependent (1–30 mg/kg) anti-inflammatory activity on carrageenan-induced mouse paw oedema (ED50= 10.1 mg/kg) and on acetic acid-induced writhing responses in mice (ED50= 13.8 mg/kg). In thein vivoexperiments, the effect of tested compound was also evaluated in presence of the reference drug diclofenac (1–30 mg/kg). Moreover,in silicoanalysis of receptor ligand complex shows that compound1interacts with cyclooxygenases (COXs) binding sites displaying an interesting interaction with COX-1. These findings suggest that compound1isolated fromP.integerrimapossessesin vivoanti-inflammatory and antinociceptive potentials, which are supportedin silicoby an interaction with COXs receptors.

scholarly journals EVALUATION OF EFFECT OF AQUEOUS EXTRACT OF PORTULACA OLERACEA LINN. LEAVES IN ACUTE INFLAMMATION

2020 ◽  
pp. 1-2
Author(s):  
M. Medhabati ◽  
L. Babycha ◽  
L. Tarinita ◽  
Bikram Tewari ◽  
Saumya Kanti Sinha
Keyword(s):  
Aqueous Extract ◽  
Acute Inflammation ◽  
Portulaca Oleracea ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Paw Oedema ◽  
The Mean ◽  
Anti Inflammatory ◽  
Inflammatory Action

In the present study, the anti-inflammatory activity of aqueous extract of Portulaca oleracea (POE 200, 400 and 600mg/kg) was studied using carrageenan induced paw oedema in albino rats. The mean increase in paw volume was recorded. POE produced significant (p<,0.01) anti-inflammatory activity when compared to the control. The anti-inflammatory action of POEcan be attributed to its flavonoid contents which are known to act through inhibition of prostaglandin biosynthesis. However, the test drug at maximum dose (600mg/kg) was found to be less effective than the standard drug, aspirin (100mg/Kg).

scholarly journals Investigation of anti-inflammatory and antipyretic activity of polymorphic modification of n-(trifluoromethylphenyl)-4-hydroxy-2,2-dioxo-1h-2λ6,1-benzothiazine-3-carboxamide derivative in rats

2021 ◽  
Vol 25 (1) ◽  
pp. 27-31
Author(s):  
P. S. Bondarenko ◽  
N. І. Voloshchuk
Keyword(s):  
Inflammatory Reaction ◽  
White Male ◽  
Statistical Processing ◽  
Biologically Active ◽  
Inflammatory Activity ◽  
Polymorphic Modification ◽  
Reference Drug ◽  
Antipyretic Effect ◽  
Antipyretic Activity ◽  
Anti Inflammatory

Annotation. Polymorphism among biologically active substances is an extremely important factor that modifies the therapeutic properties of pharmaceutical substances and dosage forms, significantly affects the parameters of their biological activity and pharmacokinetics. Chemical synthesis among the derivatives of 4-R-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxylic acids gave the leader compound N-(4-trifluoromethylphenyl)-4-hydroxy-2,2-dioxo-1H-2λ6,1-benzothiazine-3-carboxamide, the crystalline polymorph of which in the form of plates has a pronounced analgesic activity. The aim of the study was to investigate the anti-inflammatory activity of compound B by its effect on various components of the inflammatory reaction, as well as the antipyretic effect of this compound in rats. The anti-alterative activity was determined on the model of planar skin wounds, the antiproliferative effect - on the model of cotton granuloma, the anti-exudative effect - on the model of toxic pulmonary edema in rats. The antipyretic effect was studied in a model of milk fever in rats. The study was performed on 91 white male Wistar rats. Statistical processing of the obtained results was performed using the program "STATISTICA 6.1" by methods of variation statistics using parametric and nonparametric methods. The results showed that the polymorphic compound B has anti-inflammatory activity on all components of the inflammatory reaction - alteration, exudation and proliferation. In terms of efficacy in the model of experimental inflammation, this substance is superior to meloxicam, as its ED50 when administered orally is 5.2 mg/kg, while the reference drug - 9.1 mg/kg. Compound B exhibits a pronounced antipyretic effect in a rat fever model. Therefore, the polymorphic modification of the carboxamide derivative in the form of plates is promising for further research in order to create on its basis a drug with analgesic, anti-inflammatory and antipyretic properties.

scholarly journals ANTI-PYRETIC AND ANTI-INFLAMMATORY ACTIVITY OF AQUEOUS EXTRACT OFSOLANUM XANTHOCARPUM BERRIES IN SUITABLE ANIMAL MODELS

2021 ◽  
Vol 9 (11) ◽  
pp. 954-959
Author(s):  
Leisangthem Tarinita Devi ◽  
◽  
Mayanglambam Medhabati Devi ◽  
Florence Lalvarmawi ◽  
Swagata Datta ◽  
...  
Keyword(s):  
Animal Models ◽  
Aqueous Extract ◽  
Inflammatory Activity ◽  
Active Principle ◽  
Traditional Medicines ◽  
Control Drug ◽  
Paw Oedema ◽  
Study Results ◽  
Anti Inflammatory ◽  
Solanum Xanthocarpum

Background: Use of traditional medicines for treating various diseases have become a topic of global importance because of their safety, less side effects and cost-effectiveness. The present study was undertaken to evaluate the anti-pyretic and anti-inflammatory activity of aqueous extract of Solanum xanthocarpum berries (SXB) in suitable animal models. Methods: Anti-pyretic activity was assessed by dried yeast induced pyrexia in rats. Anti-inflammatory activity was evaluated using carrageenan induced paw oedema in rats. Three doses of the plant extract (500, 1000 and 1500 mg/kg) prepared by dissolving the drugs in 2% gum acacia were used. Paracetamol 33 mg/kg and aspirin 100 mg/kg were used as standard drugs for anti-pyretic and anti-inflammatory activity respectively. Vehicle served as a control drug. Results: Acute toxicity study results demonstrated no mortality of animals after 24 hours. The aqueous extract of the plant significantly decreased the rectal temperature of the rats and significantly prevented increase in volume of paw oedema. Conclusion: The aqueous extract of Solanum xanthocarpum berries exerts its anti-pyretic and anti-inflammatory activity activity. However, further studies with the plant are required to evaluate the dose dependent activity and also to determine the active principle responsible for exact mechanism for both antipyretic and anti-inflammatory activity.

scholarly journals Anti-Inflammatory Activity of Oxycarotenoid Extracts Isolated from Coriander Leaves (Coriandrum Sativum) and Curry Leaves (Murraya koenigii) on Carrageenan Induced Acute Inflammation in Rats

2021 ◽  
Author(s):  
AM ANUSHA ◽  
PA SHERENA ◽  
PT ANNAMALA ◽  
JK Mukkadan
Keyword(s):  
Body Weight ◽  
Coriandrum Sativum ◽  
Leafy Vegetables ◽  
Inflammatory Activity ◽  
Murraya Koenigii ◽  
Curry Leaves ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Coriander Leaves

Introduction: Green leafy vegetables are important sources of polyphenols and carotenoids which possess both antioxidant and anti-inflammatory activities. Aim: To study the anti-inflammatory activity of oxycarotenoid extracts isolated from coriander leaves (Coriandrumsativum) and curry leaves (Murraya koenigii) in carrageenan induced acute paw oedema in rats. Materials and Methods: Oxycarotenoid extracts were isolated from the coriander leaves (Coriandrum sativum) and curry leaves (Murraya koenigii) and they were assessed for anti-inflammatory activities by in-vivo methods. The in-vivo anti-inflammatory activity was evaluated in carrageenan induced acute paw oedema model. Indomethacin at a dose of 20 mg/Kg body weight was used as standard anti-inflammatory drug. Results: The results revealed that oxycarotenoids extracted from coriander leaves administered at a dose of 40 mg/kg body weight showed an inhibition of 53.33% whereas the oxycarotenoids extracted from curry leaves showed an inhibition of 60% at the sixth hour after carrageenan injection. The results are comparable with those of indomethacin (20 mg/Kg body weight) administered group which showed an inhibition of 55.53% Conclusion: These findings suggest that oxycarotenoid extracts isolated from leafy vegetables (coriander leaves and curry leaves) have significant anti-inflammatory activities.

scholarly journals Screening for anti-inflammatory activity of extract and fractions of Morinda lucida stem bark and detection of bioactive secondary metabolite

2021 ◽  
Vol 16 (1) ◽  
pp. 031-039
Author(s):  
Chukwubuikem C. Okolo ◽  
Nancy A. Mbachu ◽  
Ogechi O. Anyanwu ◽  
Kenneth G. Ngwoke ◽  
Festus B.C. Okoye
Keyword(s):  
Stem Bark ◽  
Positive Control ◽  
Inflammatory Activity ◽  
Paw Oedema ◽  
Dependent Inhibition ◽  
Egg Albumen ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Ear Oedema ◽  
Rat Paw Oedema

Morinda lucida Benth (Rubiaceae) is a versatile plant used in traditional medicine for the treatment of a variety of ailments and the claims of its efficacy are particularly remarkable in the treatment of infections and immuno-inflammatory disorders. This study evaluates the anti-inflammatory properties of methanolic stem bark extract and fractions of M. lucida and also identifies the phytochemicals responsible for its anti-inflammatory activity. The crude extract was subjected to liquid- liquid partitioning successively with n- hexane, ethylacetate, butanol and water. High performance liquid chromatography (HPLC) of the four fractions and Vacuum Liquid Chromatography fraction (VLC) of the promising fraction was evaluated. The effect of the fractions on egg albumen induced rat paw oedema were also evaluated. Anti-inflammatory activity of the fractions was further screened using xylene induce ear oedema models and human red blood cell membrane stabilization test. Ulcerogenic test on the normal stomach mucosa was also evaluated. The result of the egg albumen induced rat paw oedema showed that the butanol fractions maximally inhibited egg albumen induced effect at 400 mg/kg (70%) and 200mg/kg (67.5%) after 180 minutes compared to the positive control, ibuprofen (20mg/kg) with 100% inhibition after 180 minutes. The result of the xylene induced ear oedema showed that the inhibition produced by 100 µg/ear of the Butanol fraction (BF) was 56.67 % and was greater than inhibition produced by 200 µg/ear of ibuprofen (38.89 %). HPLC analysis of the fractions revealed the following phytocompounds; Cytreo- a-pyrone, Cytosporin- J and Waol A. Ulcerogenic test was negative at the doses of 200 mg/kg and 400 mg/kg of the fractions when compare with the indomethacin (positive control) at dose of 50 mg/kg. Human red blood cell membrane stabilization assay showed that BF-VLC 2 (Dichloromethane: methanol (8:2) VLC of Butanol fraction) exhibited concentration dependent inhibition of heat-induced haemolysis while other extract showed a non- concentration dependent inhibition of haemolysis when compared to the standard, ibuprofen. These findings suggest that the stem bark of M. lucida possess promising anti-inflammatory phytocompounds which justify its use in ethno-medicine.

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