scholarly journals The Effect of BAP and Kinetin Concentrations for shoot induction on Teak (Tectona grandis L.) with In Vitro method

2019 ◽  
Vol 1 (2) ◽  
pp. 93
Author(s):  
Mayang Arnindika Prameswari ◽  
Karno Karno ◽  
Syaiful Anwar
Keyword(s):  
Factorial Design ◽  
Interaction Effect ◽  
Leaf Development ◽  
Tectona Grandis ◽  
Shoot Induction ◽  
In Vitro Method ◽  
Total Growth ◽  
Number Of Leaves ◽  
Number Of Shoots

The purpose of this research was to examine interaction effect between the different concentration of BAP hormone (Benzylaminopurine) and Kinetin hormone on the shoot induction of Jati (Tectona grandis L.) with in vitro method. This research used a completely randomized factorial design with 4x3 factorial pattern. The first factor was the concentration of BAP (B0 = 0 ppm; B1 = 1 ppm; B2 = 2 ppm dan B3 = 3 ppm). The second factor was concentration of Kinetin (K0 = 0 ppm; K2 = 0,75 ppm; dan K3 = 1,5 ppm). The parameters observed were time of callus appearance, callus length, time of shoot emergance, number of shoots, length of shoots, time of leaf development, number of leaves, percentage of growth, and percentage of contamination. The parameters obtained were analysed descriptively because the percentage of total growth was under 50%. The result showed that the concentration 1 ppm BAP and 0,75 ppm kinetin was better for stimulating the appearance of callus, shoots, leaves, and number of shoots. Callus diameter was better at concentration of BAP 3 ppm. The callus texture that was formed was included in the compact texture. The percentage of explants life was 33,30 – 66,60%. No contamination occurred in all explant samples.

scholarly journals Micropropagation of Strawberry (Fragaria ananassa) through runner culture

2013 ◽  
Vol 38 (3) ◽  
pp. 467-472 ◽  
Author(s):  
M Ashrafuzzaman ◽  
SM Faisal ◽  
D Yadav ◽  
D Khanam ◽  
F Raihan
Keyword(s):  
In Vitro Propagation ◽  
Average Length ◽  
Root Induction ◽  
Fragaria Ananassa ◽  
Shoot Induction ◽  
Half Strength ◽  
Number Of Leaves ◽  
Number Of Shoots

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was observed at the concentration of 0.5 mg/l BAP. The highest average number of leaves (5) was also observed at the same concentration. Among the five rooting concentrations, IBA @ 0.5 mg/l showed the best performance in all the parameters studied. The highest number (6) of roots/culture and the longest (3.05 cm) roots were also obtained from this concentration. Half strength MS media without IBA concentration did not show any response regarding root induction. DOI: http://dx.doi.org/10.3329/bjar.v38i3.16973 Bangladesh J. Agril. Res. 38(3): 467-472, September 2013

Optimization of Honey Concentration on In Vitro Sorghum (Sorghum bicolor ) Shoot Induction

2020 ◽  
Vol 4 (2) ◽  
pp. 106-111
Author(s):  
Luluk Muzayyana ◽  
Muhammad Hazmi ◽  
Hidayah Murtiyaningsih ◽  
Laras Sekar Arum
Keyword(s):  
Functional Food ◽  
Optimum Concentration ◽  
Shoot Induction ◽  
Single Treatment ◽  
Real Difference ◽  
Study Results ◽  
Number Of Leaves ◽  
Regeneration In Vitro ◽  
Number Of Shoots

Sorghum can be developed a functional food, feeding and bioethanol. The genetically modified development is threated regeneration in vitro. The purpose study to get an optimum concentration of honey on MS media for the growth of sorghum shoots. The study was carried out using a randomized complete design with a single treatment of honey concentration, 4 replications. The concentration of honey used covers 0, 5, 10, 15 and 20 g/l. Observation parameters consist of time shoot appears, percentages emerge shoots, high shoots and number of leaves. Data obtained were analyzed by F test further more with DMRT 5%. Study results showed that there is no significantly influenced on time shoot appears, high shoot 9, 12, and 15 hsi, while the real difference in the number of shoots, percentage emerge shoot and high shoot 6 hsi. The treatment 5 g/l of honey able to accelerate time shoot appearance, and honey treatment 10 g/l result in the percentage of the highest shoot of 72%, the number of shoots 0,72 and the highest growth length 0f 5,02 mm at 6 hsi, was the best treatment.

scholarly journals PENGARUH BAP (Benzyl Amino Purine) DAN AIR KELAPA TERHADAP PERTUMBUHAN TUNAS PUCUK DAN KANDUNGAN SULFORAFAN BROKOLI (Brassica oleracea L. var. italica Plenck) SECARA IN-VITRO

2018 ◽  
Vol 14 (1) ◽  
pp. 439
Author(s):  
Alfrida ., Maninggolang ◽  
Jeany Sh. Polii-Mandang ◽  
Wenny ., Tilaar
Keyword(s):  
Brassica Oleracea ◽  
Coconut Water ◽  
Shoot Bud ◽  
Randomized Design ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Brassica Oleracea L ◽  
Benzyl Amino Purine ◽  
Number Of Shoots

This study aims to know the effect of Benzyl Amino Purine (BAP) and Coconut Water on shoot bud growth and Broccoli Sulforaphane content (Brassica oleracea L. var italic Plenck). The study was conducted in the laboratory of Biotechnology Department of Aquaculture, Faculty of Agriculture of Sam Ratulangi University, Manado, that conducted from August-December 2017. This study used a Complete Randomized Design (RAL), consisting of 8 treatments and each repeated as many 4 times, so we get 32 unit experiment. The variables observed were number of buds, number of leaves, plant height, wet weight, root number and Sulforaphane content analysis. The result of research shows that analysis of variance showed that in the use of Benzyl Amino Purine (BAP) concentration 3 ppm tends to increase the number of leaves aged 4 Weeks After Culture (MSK) and increase the number of shoots age 2 and 6 Weeks After Culture (MSK). Benzyl Amino Purine (BAP) 3 ppm can increase the wet weight of age 6W eeks After Culture ((MSK). Coconut water 20% tends to increase the number of leaves at age 6 Weeks After Culture (MSK) and increase the number of shoots aged 6 Weeks After Culture (MSK), while for combination of 3 ppm Benzyl Amino Purine (BAP) and coconut water 20% tends to increase the number of leaves aged 2 Weeks After Culture (MSK) and the number of shoots aged 2 Weeks After Culture (MSK). Combination of coconut water and Benzyl Amino Purine (BAP) is not detected by the content of Sulforaphane.

scholarly journals In vitro Regeneration and Rapid Multiplication of Dendrobium bensoniae, an Indigenous Ornamental Orchid

2017 ◽  
Vol 14 (2) ◽  
pp. 24-31 ◽  
Author(s):  
S S Riva ◽  
A Islam ◽  
M E Hoque
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Rapid Multiplication ◽  
Number Of Shoots

An experiment was conducted on in vitro regeneration and multiplication of Dendrobium bensoniae. Different concentrations of BA and IBA alone or combination of both hormones were used as treatment for regeneration.  It was revealed that shoot regeneration from node was the best at 2.0 mg/l BA supplemented to MS medium. It gave better responses than all other concentrations and combinations of BA and BA+IBA, used in the present study. The highest number of shoots and leaves were found when 1.0 mg/l BA with 1.5 mg/l IBA was supplemented into MS medium.  For rooting, 0.5 mg/l BA with 1.0 mg/l IBA was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field. From the present result, it may be recommended that MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction and regeneration of D. bensoniae.The Agriculturists 2016; 14(2) 24-31

scholarly journals THE GROWTH OF KIWI SHOOT (ACTINIDIA DELICIOSA) ON VARIOUS KINDS OF GELLING AGENTS

2019 ◽  
Vol 5 (2) ◽  
pp. 67
Author(s):  
Mardiana Mardiana ◽  
Zainuddin Zainuddin ◽  
Mahfudz Mahfudz ◽  
Hawalina Hawalina
Keyword(s):  
Shoot Growth ◽  
Good Condition ◽  
Kiwi Fruit ◽  
Physiological Maturity ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Time Required ◽  
Number Of Shoots

Kiwi fruit takes about 25 weeks from flower bloom until it reaches physiological maturity, so the time required to produce kiwi seeds from seeds in large quantities and uniform is very long. Tissue culture is one method that can be used to obtain a lot of kiwi seeds and uniforms with large quantities in a faster time. The purpose of this study was to examine various types of media compaction materials for the growth of kiwi shoots in vitro. This study was prepared based on Completely Randomized Design (RAL) with 5 treatments and repeated 4 times so that there were 20 experimental units, each experiment using 2 explants so that there are 40 eksplan. The treatments were: MA 1: Agar Swallow Globe 8 g / l, MA 2: Agar Swallow Globe 4 g / l + Agar Nutrijell 4 g / l, MA 3: Agar Swallow Globe 4 g / l + Agar Nutrijell 5 g / l, MA 4: Phytagel 2.2 g / l, MA 5: Agar Nutrijell 11 g / l. Observation variables are When shoots appear, Number of shoots, number of leaves, Number of Roots, number of root hair. The results showed Swallow Globe 4 g / l + Agar Nutrijell 4 g / l treatment gave the highest average number of shoots, the highest number of leaves and roots, this proved that the combination of Swallow Globe and Nutrijell agar gave a good condition for shoot growth kiwi plant.t.

scholarly journals Coconut Water and IAA Effect on the In Vitro Growth of Tribulus terrestris L.

2019 ◽  
Vol 67 (1) ◽  
pp. 9-18
Author(s):  
Enik Akhiriana ◽  
Samanhudi ◽  
Ahmad Yunus
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Tribulus Terrestris ◽  
Emergence Time ◽  
Shoot Height ◽  
Randomized Design ◽  
Number Of Leaves ◽  
Indole 3 Acetic Acid ◽  
Number Of Shoots

Tribulus (Tribulus terrestrisL.) is a medicinal plant with considerable implementation such as aphrodisiac and anti‑inflammation drugs. This research was conducted to study the effectivity of coconut water and IAA (Indole‑3‑acetic acid) application on various concentration as the growth regulator for tribulus growth on thein vitroand its effect toward observation variables. The material used were cotyledon from tribulus embryo and MS (Murashige and Skoog) medium. The research method used a Completely Randomized Design with first factor of coconut water concentration of 0 mL L-1, 50 mL L-1, 100 mL L-1, and 150 mL L-1and IAA concentration of 0 ppm, 0.15 ppm, 0.20 ppm, and 0.25 ppm as the second factor. Observed variables were shoots emergence time, number of shoots, shoot height, number of leaves, roots emergence time, number of roots and regression between observation variables. The result showed that the combination of 150 mL L-1coconut water and 0.25 ppm IAA gave the highest shoot height, roots emergence time, and number of roots. Coconut water treatment on 150 mL L-1concentration alone gave the best result on shoots emergence time, number of shoots, and number of leaves, while IAA concentration of 0.25 ppm independently gave the highest number of leaves. Regression analysis result indicate that the number of roots has a positive correlation with shoots height, number of shoots and number of leaves.

PEMANFAATAN BAHAN TEKNIS KNO3, CaCl2, MgSO4, KH2PO4 SEBAGAI HARA MAKRO DAN Benzil Adenin DALAM PERBANYAKAN JATI (Tectona grandis L) SECARA IN VITRO

2013 ◽  
Vol 14 (3) ◽  
Author(s):  
Karyanti Karyanti ◽  
Juwartina Ida Royani
Keyword(s):  
Mass Production ◽  
Tectona Grandis ◽  
Chemical Substance ◽  
Shoot Induction ◽  
Plant Propagation ◽  
Chemical Substances ◽  
In Vitro Technique ◽  
Major Producer ◽  
Macro Element

Indonesia is a major producer of teak after India and will gradually decrease if not followed by replanting. In general, teak plants propagated through seeds but have many disadvantages. Teak plant propagation using in vitro technique being an alternative to get the mass production of teak clones. In vitro technique, to some extent, needs a high cost particularly in using pure chemical substance. As an alternative solution, pure chemical substance can be substituted by using a few technique chemical substances such as KNO3, CaCl2, MgSO4, and KH2PO4. The aim of this research was to evaluated induction of shoot teaks, planted on different media with macro element substituted by different chemicals such as KNO3, CaCl2, MgSO4, and KH2PO4. The result showed that using the same concentration between 2 different chemical substances on teak shoot induction, there was no different shoot growing in teak propagated between 2 media.

scholarly journals RUNNER-TIP CULTURE OF STRAWBERRY (Fragaria x ananassa Duch) GROWN ON SEVERAL SHOOT-INDUCTION MEDIUM

2020 ◽  
Vol 8 (1) ◽  
pp. 10
Author(s):  
Rindang Dwiyani ◽  
Hestin Yuswanti ◽  
Ni Nyoman Ari Mayadewi ◽  
Yuyun Fitriani
Keyword(s):  
Fragaria X Ananassa ◽  
Induction Medium ◽  
Shoot Induction ◽  
Suitable Medium ◽  
Bud Emergence ◽  
Randomized Design ◽  
Shoot Production ◽  
Number Of Leaves ◽  
Shoot Induction Medium ◽  
Number Of Shoots

 A research regarding “Runner-tip culture of strawberry (Fragaria x ananassa Duch) Grown on Several Shoot-induction Medium” has been investigated. The objective of the research was to find out the most suitable medium for shoot production from runner-tip culture of strawberry at establishment step of micropropagation. The research was laid out in a Completely Randomized Design, 4 treatments of medium type for shoot induction and 10 replication, each was represented by one (1) bottle with 6-8 explants. The treatments were summarized as follows: T1 = MS ; T2 = MS + 2 ppm BAP + 0.01 ppm NAA; T3 = MS + 1 ppm of TDZ; T4 = WPM + 2 ppm BAP + 0.01 ppm NAA. The parameters observed were days of the bud emergence, the average number of shoots per explant, and the average number of leaves per explant. It can be concluded that among medium used in the current research, the medium of MS added with 1 ppm thidiazuron (TDZ) was the most suitable medium for shoot production of strawberry from the explant of runner-tips. The treatment was resulted in the earliest time of bud emergence, and producing the highest number of shoots and leaves.

Regeneration of Medicinal Plant Paederia foetida through Direct Organogenesis using Nodal Explants

2021 ◽  
Vol 24 (1) ◽  
pp. 89-98
Author(s):  
SH Binto ◽  
JU Ahmed ◽  
TK Ghosh
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Surface Sterilization ◽  
Paederia Foetida ◽  
Number Of Leaves ◽  
Number Of Shoots

Chinese fever vine (Paederia foetida L.), a valuable medicinal plant has been greatly utilized in therapeutic purposes throughout the world. Since conventional propagation techniques of P. foetida are very slow, inefficient and cannot cope with the increasing demand, in-vitro regeneration through tissue culture could be an alternative means of rapid propagation. Therefore, the efforts were made to develop a suitable protocol through direct organogenesis of P. foetida. After surface sterilization, the nodal explants were cultured in Murashigue and Skoog (MS) medium and MS medium supplemented with different concentrations and combinations of plant growth regulators. MS medium supplemented with 6-benzylaminopurine; BAP (2.0 mg L-1) produced the maximum number of shoots; 4.40 ± 0.98 and 5.40±1.12 after 15 and 30 days of culture respectively. The number of shoots gained by 15 days was found to be the highest; 1.20±0.80 at BAP (4.0 mg L-1) followed by 1.00±0.55 at BAP (2.0 mg L-1). Although the combination of BAP + Kinetin (2 mg L-1 +2 mg L-1) showed the highest shoot growth (3.40 ± 1.08 cm) by 15 days, sole application of BAP (2.0 mg L-1) or Kn (0.5, 1.0, 2.0 and 3.0 mg L-1) showed similar responses. BAP (2.0 mg L-1) showed the best responses for developing the highest number of leaves; 18.60 ± 2.42 and 29.20 ± 2.73 respectively after 15 and 30 days of culture. Similarly, development of the maximum number of leaves (10.60 ± 0.68) was reported by 15 days at BAP (2.0 mg L-1). Rooting was significantly induced in indole-3-acetic acid (IAA) supplemented to 1/2 strength MS medium as compared to control (only ½ strength MS medium). The best performance of rooting was observed by 0.5 mg L-1 IAA which produced average 4.33 roots per shoot after 21 days of culture. The regenerated plants showed similar morphology to the mother plants. Thus, a suitable protocol for successful multiplication of P. foetida in vitro was established using nodal explants. Ann. Bangladesh Agric. (2020) 24(1) : 88-98

scholarly journals In vitro regeneration and induction of mutation in Loquat (Eriobotrya japonica L.)

2019 ◽  
pp. 701-708
Author(s):  
Muhammad Imam Surya ◽  
Lily Ismaini ◽  
Intani Quarta Lailaty
Keyword(s):  
Gamma Rays ◽  
Growth Traits ◽  
In Vitro Regeneration ◽  
Eriobotrya Japonica ◽  
In Vitro Mutagenesis ◽  
Induced Mutations ◽  
Apical Shoot ◽  
Number Of Leaves ◽  
Number Of Shoots

Loquat (Eriobotrya japonica L.) is a potential fruit crop in Indonesia. The experiments were carried out to study the effect of gamma rays as in vitro mutagenesis agent and also the regeneration of mutant explants of loquat on different media. The experiment used two media (MS and WPM) containing BA and NAA, three type of explants (apical shoot, stem, cotyledon), and four doses of gamma rays (0, 10, 30, 50 Gy). The results showed that MS media was better than WPM media in term of callus production. Moreover, explants of loquat showed a different response to gamma irradiation and the highest response on number of shoots, number of leaves and plant height was found at a dose of 10 Gy. On the other hand, the explants of cotyledon were only able to produce callus. The explants of the apical shoot were able to produce the highest number of leaves and shoots than the explants of the stem. However, the combination of MS media, apical shoot explants and 10 Gy of gamma rays irradiation showed a better response in the growth traits of callus diameter, callus height, number of shoots, number of leaves and plantlet height. Overall, the results of this study showed that in vitro culture in combination with induced mutations can speed up breeding programmes.

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