scholarly journals Wound Healing Activity of Calotropis Procera Root Bark on Diabetic Rats

2020 ◽  
Vol 10 (2-s) ◽  
pp. 86-89
Author(s):  
Rohit P. Mali ◽  
Priya S. Rao ◽  
D.N. Vikhe
Keyword(s):  
Wound Healing ◽  
Traditional Medicine ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Calotropis Procera ◽  
Root Bark ◽  
Diabetic Wound ◽  
Soxhlet Method ◽  
Developed World ◽  
Wound Healing Activity

Herbal therapy and herbal medicine in traditional medicine as well as in alternative medicine practiced in the developed world. There is recent most of the harbal formulation are used to cure & improve the humal life. The present study Wound healing activity of Calotropis procera root bark on diabetic rats in that root bark is extracted from ethanolic extract using soxhlet method. The pharmacognostic study was done after that phytochemical characters should be studied. The root bark has alkaloids, Flavonoids, steroids and tannins active constituents.  Acute toxicity study carried out with three pharmacological models Incision model, Excision model, and Dead space model. All the extract show predominant activity against selected species. Extract shows beneficial effect on diabetic wound it heals in time with standard component. Overall results of this study reveals that this is an effective extract on diabetic wound. Keywords: Traditional medicine, Calotropis procera, Wound Healing Activity

Evaluation of Effect of Biologically Synthesized Ethanolic Extract of Propolis-Loaded Poly(-Lactic-co-Glycolic Acid) Nanoparticles on Wound Healing in Diabetic Rats

2022 ◽  
pp. 153473462110732
Author(s):  
Reza Tayfeh-Ebrahimi ◽  
Amir Amniattalab ◽  
Rahim Mohammadi
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Granulation Tissue ◽  
Glycolic Acid ◽  
Ethanolic Extract ◽  
Plga Nanoparticles ◽  
Diabetic Rats ◽  
Diabetic Wound ◽  
Wound Size ◽  
Ethanolic Extract Of Propolis

Wound healing is interaction of a complex cascade of cellular/biochemical actions leading to restoration of structural and functional integrity with regain of injured tissues strength. This study was aimed at evaluation of application of ethanolic extract of propolis-loaded poly(-lactic-co-glycolic acid) nanoparticles (EEP-PLGA NPs) on wound healing in diabetic rats. Sixty rats were randomized into four groups of 15 rats each: In control group (Control) diabetic wound was treated with normal saline. In Carrier 1 group diabetic wound was treated with PLGA nanoparticles based solution. In Carrier 2 group the diabetic wound was treated with EEP. In Treatment group animals received EEP-PLGA NPs on the wound. Wound size was measured on 7, 14 and 21 days after surgery. The expression of p53, bcl-2, Caspase III, were evaluated using reverse-transcription PCR and Immunohistochemical staining. The Treatment group had significantly reduced the wound size compared to other groups ( P = 0.001). histological and morphometric studies, and mean rank of the qualitative studies demonstrated that there was significant difference between Treatment group and other groups ( P < .05). Observations demonstrated that ethanolic extract of propolis-loaded PLGA nanoparticles significantly shortened the inflammatory phase and accelerated the cellular proliferation. Accordingly, the animals in Treatment group revealed significantly ( P < .05) higher fibroblast distribution/one mm2 of wound area and rapid re epithelialization. The mRNA levels of bcl-2, p53 and caspase III were remarkably ( P < .05) higher in Treatment group compared to control and animals. The immunohistochemical analyzes confirmed the RT-PCR findings. EEP-PLGA NPs offered potential advantages in wound healing acceleration and improvement through angiogenesis stimulation, fibroblast proliferation and granulation tissue formation in early days of healing phases, acceleration in diabetic wound repair associated with earlier wound contraction and stability of damaged area by rearrangement of granulation tissue and collagen fibers.

scholarly journals Pilot Study with regard to the Wound Healing Activity of Protein fromCalotropis procera(Ait.) R. Br.

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Ramar Perumal Samy ◽  
Vincent T. K. Chow
Keyword(s):  
Wound Healing ◽  
Traditional Medicine ◽  
Stem Bark ◽  
Scientific Basis ◽  
Positive Control ◽  
Calotropis Procera ◽  
Skin Cells ◽  
Spectrum Activity ◽  
Wound Healing Activity ◽  
Broad Spectrum Activity

We provide the scientific basis for the use ofCalotropis procerafor treating skin and wound infections in traditional medicine. The aqueous extract of stem-bark ofC. proceraexhibited more pronounced potent antimicrobial activity. Calo-protein was purified and identified from the most-active aqueous extracts ofC. proceraand showed broad-spectrum activity. Calo-protein inhibited the growth ofS. aureusandE. aerogeneseffectively at 25 μg/ml concentration. Mice topically treated with Calo-protein revealed significant wound healing after 14 days comparable to fusidic acid (FA) as positive control. This protein was devoid of cytolytic effect even at higher concentrations on skin cells after 24 h. Further investigation of this Calo-protein ofC. proceraon bacterial inhibition may provide a better understanding of the scientific basis and justification for its use in traditional medicine.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals Wound healing activity of Conyza canadensis (L.) Cronquist

2018 ◽  
Vol 1 (T5) ◽  
pp. 35-40
Author(s):  
To Dinh Le ◽  
Nga Thi Hoang ◽  
Nhan Thi Thanh Nguyen ◽  
Thuoc Linh Tran ◽  
Thao Thi Phuong Dang
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
National Park ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Conyza Canadensis ◽  
Keratinocyte Proliferation ◽  
Wound Healing Activity ◽  
Stimulation Of

Conyza canadensis (L.) Cronquist (CCL) has been used by K’Ho minority in Bidoup Nui Ba national park, Lam Dong province, Vietnam as one of wound healing remedies. However, the scientific proof of treatment is still unclear. This study aimed to evaluate this wound healing potential of CCL. CCL power was extracted by ethanol and then partitioned consecutively with petroleum ether, ethyl acetate and water. Wound healing potential was evaluated by antibacterial activity, stimulation of fibroblast and keratinocyte proliferation. Agar-well diffusion was used in the antibacterial tests and the results showed that CCL had antibacterial activity against 02 dermatitis bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and 02 opportunistic infection bacteria (Escherichia coli, Enterococcus faecalis). Moreover, our results illustrated that CCL stimulated the fibroblast and keratinocyte proliferation compared to the control. Particularly, the fibroblast division increased 1.6 times at 31.25 µg/mL when treated by ethanolic extract, while ethyl acetate fraction showed 1.7 times increase at 10 µg/mL in keratinocyte proliferation compared to the control. Taken together, our study contributed scientific base of CCL in the wound healing.

scholarly journals Wound healing activity in rabbits and antimicrobial activity of Hibiscus hirtus ethanolic extract

2018 ◽  
Vol 54 (4) ◽  
Author(s):  
Kakaraparthy Ravishankar ◽  
Garlanka Venkata Naga Kiranmayi ◽  
Yejella Rajendra Prasad ◽  
Lankadi Devi
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Ethanolic Extract ◽  
Wound Healing Activity

Wound-healing activity of an oligomer of alkannin/shikonin, isolated from root bark ofOnosma echioides

2015 ◽  
Vol 29 (16) ◽  
pp. 1584-1588 ◽  
Author(s):  
Gawand Nikita ◽  
Purnima Vivek ◽  
Gadgoli Chhaya
Keyword(s):  
Wound Healing ◽  
Root Bark ◽  
Wound Healing Activity

scholarly journals Antihyperglycemic and diabetic wound healing activity of smallanthus sonchifolius leaves extract

2018 ◽  
Vol 197 ◽  
pp. 07001
Author(s):  
Rina Herowati ◽  
Alip D.S. Saputri ◽  
Tri Wijayanti ◽  
Gunawan P. Widodo
Keyword(s):  
Wound Healing ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Topical Administration ◽  
Serum Glucose ◽  
Diabetic Wound ◽  
Smallanthus Sonchifolius ◽  
Biopsy Punch ◽  
Diabetic Wound Healing ◽  
Wound Healing Activity

Diabetic patient tend to suffer from lower extremity complication that contribute to the occurence of diabetic foot ulcer. Smallanthus sonchifolius or yacon leaves extract has been used as an alternative medicine for diabetes and also showed antibacterial activity. This research aims to obtain the antihyperglycemic and diabetic wound healing activity of yacon leaves extract. Diabetic condition of rat was induced by streptozotocin (45 mg/kg bw) and nicotinamide (110 mg/kg bw). Rats were then allowed to develop diabetes for 21 days. A biopsy punch then was used to create a wound. Yacon leaves extract were administered by oral and topical for 14 day. The serum glucose level and diameter of the wound were measured every week, and histopatology study of the skin was conducted at the end of the study. Oral administration of yacon leaves extract (150 mg/kg bw) together with topical administration showed antihyperglycemic as well as diabetic wound healing activities comparable to the reference drugs (glibenclamide oral and nebacetin cream).

scholarly journals Experimental Evaluation of Ethanolic Extract ofCarapa guianensisL. Leaf for Its Wound Healing Activity Using Three Wound Models

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
B. Shivananda Nayak ◽  
Joel Kanhai ◽  
David Malcolm Milne ◽  
Lexley Pinto Pereira ◽  
William H. Swanston
Keyword(s):  
Wound Healing ◽  
Drinking Water ◽  
Granulation Tissue ◽  
Dead Space ◽  
Breaking Strength ◽  
Test Group ◽  
Ethanolic Extract ◽  
Wound Contraction ◽  
Hydroxyproline Content ◽  
Wound Healing Activity

The leaves ofCarapa guianensishave been used to treat ulcers, skin parasites, and skin problems. The ethanolic extract ofC. guianensisleaf was evaluated for its antibacterial and wound healing activity using excision, incision and dead space wound models in rats. The animals were randomly divided into two groups (n= 6) in all the models. In the excision wound model test group animals were treated topically with the leaf extract (250 mg kg−1body weight) whereas, control animals were treated with petroleum jelly. In the incision and dead space wound models, the test group animals were treated with extract (250 mg kg−1day−1) orally by mixing in drinking water and the control group animals were maintained with plain drinking water. Healing was assessed by the rate of wound contraction, period of epithelialization, skin breaking strength, granulation tissue weight and hydoxyproline content. On Day 15 extract-treated animals exhibited 100% reduction in the wound area when compared to controls (95%) with significant decrease in the epithelialization period. The extract failed to demonstrate antibacterial activity. Skin breaking strength (P< .001), wet (P< .002) and dry (P< .02) granulation tissue and hydroxyproline content (P< .03) were significantly higher in extract treated animals. The increased rate of wound contraction, skin breaking strength and hydroxyproline content supports potential application ofC. guianensisin wound healing.

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