scholarly journals Wound healing activity of Conyza canadensis (L.) Cronquist

2018 ◽  
Vol 1 (T5) ◽  
pp. 35-40
Author(s):  
To Dinh Le ◽  
Nga Thi Hoang ◽  
Nhan Thi Thanh Nguyen ◽  
Thuoc Linh Tran ◽  
Thao Thi Phuong Dang
Keyword(s):  
Wound Healing ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
National Park ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Conyza Canadensis ◽  
Keratinocyte Proliferation ◽  
Wound Healing Activity ◽  
Stimulation Of

Conyza canadensis (L.) Cronquist (CCL) has been used by K’Ho minority in Bidoup Nui Ba national park, Lam Dong province, Vietnam as one of wound healing remedies. However, the scientific proof of treatment is still unclear. This study aimed to evaluate this wound healing potential of CCL. CCL power was extracted by ethanol and then partitioned consecutively with petroleum ether, ethyl acetate and water. Wound healing potential was evaluated by antibacterial activity, stimulation of fibroblast and keratinocyte proliferation. Agar-well diffusion was used in the antibacterial tests and the results showed that CCL had antibacterial activity against 02 dermatitis bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and 02 opportunistic infection bacteria (Escherichia coli, Enterococcus faecalis). Moreover, our results illustrated that CCL stimulated the fibroblast and keratinocyte proliferation compared to the control. Particularly, the fibroblast division increased 1.6 times at 31.25 µg/mL when treated by ethanolic extract, while ethyl acetate fraction showed 1.7 times increase at 10 µg/mL in keratinocyte proliferation compared to the control. Taken together, our study contributed scientific base of CCL in the wound healing.

WOUND HEALING ACTIVITY OF THE WHOLE PLANT OF MOMORDICA CHARANTIA LINN. IN RATS

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (11) ◽  
pp. 64-70
Author(s):  
M.P Singh ◽  
◽  
Y.S. Sarangdevot ◽  
S.S. Sisodia
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Alcohol Extract ◽  
Whole Plant ◽  
Healing Property ◽  
Aqueous Ethanolic Extract ◽  
Wound Healing Activity

The aim of the present study was to explore the effect of wound healing activity of the whole plant of Momordica charantia Linn. in rats. The effect of aqueous ethanolic extract of Momordica charantia Linn. (Family; Cucurbetaceae) was evaluated on resutured incision, granuloma pouch and excision wound models in rats. The wound healing activity was measured by the rate of wound contraction, skin breaking strength, weight of the granulation tissue, and collagen content. The concentrated ethyl alcohol extract was subject to fractionation by using petroleum ether (40-60o V/V), solvent ether, ethyl acetate, butanol, butanone, ethanol and methanol in succession. All the fractions were screened for wound healing property. The results of pharmacological activity led to the conclusion that the petroleum ether fraction exhibited more significant wound healing promotion property followed by solvent ether and butanone, from all the parameters studied. Ethyl acetate, ethanol and methanol fractions were least effective.

scholarly journals SKRINING AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK ETANOL KERNEL BIJI BUAH LIMUS (Mangifera foetida Lour.) TERHADAP Staphylococcus aureusDAN Escherichia coli

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Vera Nurviana
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Water Fraction ◽  
Tetracycline Hcl

<p class="Default"><em>Limus (Mangifera foetida Lour.) Is one of the local Indonesian fruits of the anacardiaceae family. Limus contain secondary metabolites that have pharmacological effects, including the kernel waste of the seeds of the fruit. This study aims to determine the antibacterial activity against Staphylococcus aureus and Escherichia coli on the fraction of kernel seed of limus fruits ethanolic extract in invitro using solid diffusion method with positive control Tetracycline HCl. The extraction process was carried out by maceration method, then fractionation was obtained to obtain the fraction of n-hexane, ethyl acetate fraction and water fraction. Based on the result of the research, the fraction of n-hexane, ethyl acetate fraction, and water fraction of kernel ethanol extract limus fruit seed have antibacterial activity against Staphylococcus aureus and Escherichia coli. The highest activity was shown by fraction of ethyl acetate with minimum killing concentration (KBM) to Staphylococcus aureus was 5% with diameter equal to 4,33 ± 1.17 mm equal to 30,27 μg / mL Tetracycline HCl, and KBM value to Escherichia coli.4% (7.60 ± 0.14) mm equivalent to 26, 04 μg / mL Tetracycline HCl.<br /> <br /> <strong></strong></em></p><p class="Default"><strong><em>Keywords: </em></strong><em>Antibacterial, Staphylococcus aureus, Escherichia coli, Kernel, M. foetida.</em></p>

scholarly journals ASSESSMENT OF PHYSICOCHEMICAL PROPERTIES AND PHYTOCHEMICAL CONSTITUENTS ALONG WITH ANTIBACTERIAL POTENTIAL OF TARENNA POLYCARPA LEAF FRACTIONS

2018 ◽  
Vol 11 (4) ◽  
pp. 78
Author(s):  
Bunga Rimta Barus ◽  
Panal Sitorus ◽  
Masfria Masfria
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Physicochemical Analysis ◽  
World Health ◽  
Phytochemical Screening ◽  
Phytochemical Constituents ◽  
Health Organization ◽  
Better Than

 Objective: The aim was to investigate the physicochemical analysis, preliminary phytochemical screening and further evaluation of its antibacterial activity from morbesi-besi (Tarenna polycarpa) leaf ethyl acetate and hexane fractions (HF).Methods: The physicochemical parameters were carried out as per the World Health Organization (WHO) guideline from its ethanolic extract. The phytochemical screening was performed as per Indonesia Pharmacopoeia and conventional method on its ethyl acetate and HF. Ethyl acetate and HF at different concentration were evaluated using agar well diffusion assay.Result: The plant was found to be free from contaminations. Morbesi-besi leaf fractions are composed of flavonoids, glycosides, saponins, tannins, steroids, and triterpenoids. The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The antibacterials assay showed that ethyl acetate fraction was better than HF from the inhibition zone.Conclusion: From the outcomes of this study, morbesi-besi leaf (T. polycarpa) possess potential antibacterial activity.

scholarly journals Antibacterial Activities of Essential Oil and Fractions of Ethanolic extract of Echinops kebericho Tuber

2020 ◽  
Author(s):  
Serawit Deyno ◽  
Derick Hope ◽  
Joel PhD Ba ◽  
Eyasu Makonnen ◽  
Paul E. Alele
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Assay Method ◽  
Chloroform Fraction ◽  
E Coli ◽  
Routes Of Administration

Abstract Background: Echinops kebericho is an endemic medicinal plant in Ethiopia traditionally used for treatment of various infectious diseases through different routes of administration such as inhalation, orally chewed, and topically sprayed to affected area. This study investigated antibacterial activity of the essential oil (EO) and the different fractions of ethanolic extract of Echinops kebericho tuber.Results: MIC of EO ranged from 78.125 µg/ml – 625 µg/ml and it was most active against methicillin resistant S. aureus (MRSA, NCTC 12493). Ethyl acetate fraction showed better activity against MRSA (NCTC 12493), MIC = 39.075 µg/ml followed by E. faecalis (ATCC 49532), MIC = 78.125 µg/ml and was least active against K. pneumonia (ATCC 700603), MIC = 1250 µg/ml. MIC of hexane fraction ranged from 156.2- µg/ml to E. faecalis (ATCC 49532) to 1250 µg/ml to E. coli (NCTC 11954). Chloroform fraction MIC ranged from 312.5 - 2500µg/ml; while butanol fraction could be considered pharmacologically inactive as its MIC value is 2500 µg/ml for all and no activity shown for E. coli (NCTC 11954). The MIC of DMSO against all strains ranged 12.5- 25 % w/v. The MIC estimated by OD measurement correlated well with that of resazurin assay method.Conclusion: Essential oil and ethyl acetate fraction showed noteworthy antibacterial activity, and MRSA was the most susceptible strain. Further study, however, should be done to investigate the effect on the isolated active component(s).

scholarly journals ANTIBACTERIAL ACTIVITY OFMALACCA FRUIT (PHYLLANTUS EMBLICA L.)ETHANOLIC EXTRACT AND FRACTIONAGAINSTBACILLUS CEREUS FNCC0057 ANDSHIGELLA DYSENTERIAE ATCC13313

2017 ◽  
Vol 10 (14) ◽  
pp. 8
Author(s):  
Tiana Milanda ◽  
Arif Satria Wira Kusuma ◽  
Kuganesh Shanmuganathan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antibacterial Activities ◽  
Shigella Dysenteriae ◽  
Water Fraction ◽  
Dried Fruit

Diarrhea is a symptom of a disease, which is characterized by increased frequency of defecation (more than three times a day) with a more fluid of feces. In Ayurvedic medicine, the fruit of Malacca (Phyllanthus emblica L.) is often used as an antibacterial and antiviral against various infectious diseases. This study aims to determine:(i) the antibacterial  activities of ethanol extract and its fraction of Malacca fruit, and (ii) determine Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against diarrhea-causing bacteria, such as Bacillus cereus FNCC0057 and Shigella dysenteriae ATCC13313. The study was  conducted through several processes: (i) the determination of the plant and  preparation dried fruit, (ii) extraction of dried fruit, fractionated extract, antibacterial activity test of ethanol extract and its fractions, (iii) determination MIC and MBC from the most active fractions and (iv) phytochemical screening of fraction. The results showed that ethanol extract, water fraction and ethyl acetate  fraction of Malacca fruit has antibacterial activities against B. cereus FNCC0057 and S. dysenteriae ATCC13313, where the greatest activity was shown by the fraction of ethyl acetate. This fraction has MIC and MBC values of 0.187 - 0.375% (w/v) and and 0.09375 - 0.1875% (w/v) against B. cereus FNCC0057 and S. dysenteriae ATCC13313. The antibacterial activity of this fraction was probably derived from alkaloid, flavonoid, tannin, quinone, saponin, monoterpene or sesquiterpene compounds. 

scholarly journals Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong (Anredera cordifolia (Tenore) Steenis)

2018 ◽  
Vol 1 (3) ◽  
pp. 158-161
Author(s):  
Trie Yuni Elfasyari ◽  
Kintoko Kintoko ◽  
Nurkhasanah Nurkhasanah
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Healing Process ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Punch Biopsy ◽  
Negative Control ◽  
Diabetic Rat ◽  
Wound Healing Process ◽  
Wound Healing Activity

Proses penyembuhan luka kulit dipengaruhi oleh kondisi dibetes mellitus (DM). Ekstrak etanol daun binahong dilaporkan dapat mempercepat penyembuhan luka tikus diabetes melalui mekanisme reepitelisasi dan kolagenasi. Penelitian ini dilakukan untuk mengetahui gambaran penyembuhan luka diabetes fraksi etil asetat daun binahong (Anredera cordifolia (Ten.) Steenis) konsentrasi 10% dengan parameter jumlah sel fibroblast pada tikus yang diinduksi sterptozotocin. Metode fraksinasi yang digunakan fraksinasi bertingkat padat cair dengan pelarut etil asetat yang bersifat semipolar. Penelitian eksperimental menggunakan 20 ekor tikus Wistar jantan usia 2 bulan, berat badan antara 150-200 gram yang diinduksi streptozotocin (STZ) dosis tunggal 45 mg/kgBB i.p. Punch biopsy digunakan untuk membuat luka pada kulit tikus dengan diameter 5 mm setelah kadar glukosa darah (KGD) di atas 250 mg/dL. Hewan uji dibagi menjadi 4 kelompok, terdiri dari kontrol sehat (tanpa induksi STZ dan tanpa perlakuan), kontrol negatif (biocream), kontrol positif (madecassol), dan fraksi etil asetat daun binahong 10% (dalam biocream). Aplikasi topikal dilakukan 2 kali sehari sebanyak 25 mg selama 10 hari pada area luka dan sekitarnya. Pengamatan dilakukan secara makroskopis dan mikroskopis. Penelitian menunjukkan bahwa fraksi etil asetat daun binahong mengandung senyawa flavonoi dan tanin, serta berperan dalam meningkatkan jumlah sel fibroblast (P<0.05).   Wound healing process is affected by diabetes melitus condition. The ethanol extract of Binahong leaves has reported to have wound healing activity on diabetic rat through mechanism of re-epithelialization and collagenation. This study was conducted to evaluate the wound healing activity on diabetic rat of ethyl acetate fraction of  Binahong (Anredera cordifolia (Tenore) Steenis) leaves at concentration of 10% with parameters of fibroblast numbers in streptozozin induced rat. The fractionation method used was solid-liquid graded fractination using ethyl acetate as semipolar solvent. This experimental research used 20 male wistar rats, aged 2 months, weight 150-200 g, induced by single dose streptozotocin (STZ)45 mg/kgBB i.p. Punch biopsy was used to injured the rat skin with diameter of 5 mm after blood glucose level was more than 250 mg/dL. Test animals were divided into 4 groups, these include healthy control (without induced by STZ and treatment), negative control (biocream), positive control (madecassol), ethyl acetate fraction of binahong leaves 10% (in biocream). The cream was applied topically 2 times a day with an amount of 25 mg for 10 days on wound area and surrounding. The macroscopic and microscopic observation was performed. The results showed that ethyl acetate fraction of binahong leaves contained flavonoids and tannin, besides it plays role to increase the fibroblast numbers  (P<0.05).

COMPARATIVE EVALUATION OF IN VITRO ANTIMICROBIAL AND IN VIVO WOUND HEALING ACTIVITY OF ETHANOLIC AND ETHYL ACETATE EXTRACTS OF ABUTILON INDICUM ROOT

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (04) ◽  
pp. 21-31
Author(s):  
K Ravishankar ◽  
G.V.N Kiranmayi ◽  
J. M. Sruthi ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Wound Healing Activity ◽  
Ethyl Acetate Extracts ◽  
Abutilon Indicum

The present study was aimed at comparing the in vivo wound healing and in vitro antibacterial activity of Abutilon indicum roots. Wound healing is a complex phenomenon which consists of three stages inflammatory, proliferative, and remodelling. Traditionally, A. indicum is used for wound healing. There is no specific scientific data available for the wound healing activity of Abutilon indicum root. The present study was designed to investigate the same. The wound healing activity of ethanolic and ethyl acetate extracts of A. indicum was evaluated in incision and burn wound models. The parameters studied include tensile strength, epithelialisation period and rate of wound contraction. A. indicum also possesses antimicrobial activity. Ethyl acetate and ethanolic extract of A. indicum was tested against both Gram positive, Gram negative and fungal organisms using agar well diffusion method. From the results, it was concluded that ethyl acetate extract of A. indicum root had greater wound activity and antimicrobial activity than the ethanolic extract.

scholarly journals Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus

2021 ◽  
Vol 9 (1) ◽  
pp. 48
Author(s):  
Afif Rifqie Maulana ◽  
Bawon Triatmoko ◽  
Mochammad Amrun Hidayat
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacterial Resistance ◽  
Ethanolic Extract ◽  
Ethyl Acetate Fraction ◽  
World Health ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Water Fraction

Infection is one of the main causes of world health problems, especially in developing countries like Indonesia, one of which is an acute respiratory infection caused by bacteria such as Staphylococcus aureus. Therapeutics are used for the treatment of infections today with antibiotics. However, there are many cases of bacterial resistance to antibiotics that need to develop alternatives derived from plants. One of the plants that have antibacterial activity is the leaves of Waru Gunung (Hibiscus macrophyllus). The purpose of this study was to determine whether the ethanolic extract of H. macrophyllus leaves and their fractions have antibacterial activity against S. aureus. The method used in this antibacterial activity test is the disk diffusion method with a test concentration of 5%, 10%, 15%, 20%, and 25%. The results of this study indicate that all test samples have antibacterial activity except in the water fraction. The highest antibacterial activity was obtained in the ethyl acetate fraction which attracted semipolar compounds in the ethanolic extract of H. macrophyllus leaves. The antibacterial activity of the test samples in sequence from high to low includes ethyl acetate fraction, ethanolic extract, n-hexane fraction, and water fraction.

scholarly journals Antibacterial Activity Test of Extracts and Fractions of Cassava Leaves (Manihot esculenta Crantz) against Clinical Isolates of Staphylococcus epidermidis and Propionibacterium acnes Causing Acne

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Resmi Mustarichie ◽  
Sulistiyaningsih Sulistyaningsih ◽  
Dudi Runadi
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Staphylococcus Epidermidis ◽  
Propionibacterium Acnes ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Clinical Isolates ◽  
Active Fraction ◽  
Cassava Leaves ◽  
Ethanol Extracts

This study is aimed at determining antibacterial activity from ethanol extracts and the most active fraction of cassava leaves against clinical isolates of Staphylococcus epidermidis and Propionibacterium acnes. Research carried out by the experimental method involved determination of plants, extraction with maceration method, fractionation with liquid-liquid extraction, antibacterial activity testing of extracts and fractions by agar diffusion method, determination of most active fraction from the extract, and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) testing of most active fraction by microdilution method. The results showed that ethanol extracts of cassava leaves had antibacterial activity against both bacteria with the most active fraction indicated by ethyl acetate. MIC values of ethyl acetate fraction against S. epidermidis were in the concentration range of 2.5%–5.0% (w/v) and against P. acnes were in the concentration range of 1.25%–2.5% (w/v). The MBC value of ethyl acetate fraction against S. epidermidis was at a concentration of 5% (w/v), while P. acnes was at a concentration of 2.5% (w/v). From the results of this study, it can be concluded that the ethanol extract of cassava leaves (Manihot esculenta Crantz) has antibacterial activity against clinical isolates of Staphylococcus epidermidis as well as on Propionibacterium acnes. The fraction with the best activity from the ethanol extract of cassava leaves to the two test bacteria was shown by ethyl acetate fraction. It is suggested that cassava leaves are possible to be developed into standardized antiacne herbal.

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