scholarly journals Aislamiento de microRNAs a partir de células clorofílicas de Bouteloua gracilis y su caracterización in silico

2016 ◽  
Vol 7 (3) ◽  
pp. 263
Author(s):  
Perla Lucía Ordóñez-Baquera ◽  
Everardo González Rodríguez ◽  
Gerardo Armando Aguado Santacru ◽  
Quintín Rascón Cruz ◽  
Eduviges Burrola Barraza

Bouteloua gracilis es un pasto nativo del norte de México, que es utilizado como fuente de forraje para el ganado por su alto contenido nutritivo; tiene elevada tolerancia al estrés osmótico que le permite vivir en climas secos; sin embargo, los mecanismos moleculares que le confieren esta tolerancia aún no se han reportado. Existe una clase de RNAs pequeños (sRNAs) llamados microRNAs (miRNAs), que se unen por complementariedad a RNAs mensajeros blanco, etiquetándolos para su degradación o supresión de la traducción. En este trabajo se reporta el aislamiento de sRNAs de B. gracilis, a través de la clonación de concatámeros y su secuenciación. El análisis in silico de la secuencias, permitió la identificación de miRNAs conservados en B. gracilis y reportados en Populus trichocarpa, Brachypodium distachyon, Oryza sativa, Sorghum bicolor, Zea mays, Malus domestica y Linum usitatissimum.Además, se predijo la estructura secundaria de los precursores de dos miRNAs (pre-miRNAs), usando como referencia los genomas de Glycine max, Zea mays, Sorghum bicolor y Oryza sativa. Finalmente, se identificaron seis mRNAs blanco para uno de ellos. La identificación de miRNAs en Bouteloua gracilis, ayudará a comprender como estas moléculas regulan la expresión genética en esta especie, y sus relaciones con los mecanismos de respuesta a estrés ambiental.

2020 ◽  
Vol 11 (1) ◽  
pp. 9-16
Author(s):  
Suntoro SUNTORO ◽  
◽  
Mujiyo MUJIYO ◽  
Hery WIDIJANTO ◽  
Ganjar HERDIANSYAH ◽  
...  

Weed Science ◽  
1981 ◽  
Vol 29 (3) ◽  
pp. 256-261 ◽  
Author(s):  
R. W. Bovey ◽  
R. E. Meyer

Triclopyr {[(3,5,6-trichloro-2-pyridinyl)oxy]acetic acid}, 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid], and 3,6-dichloropicolinic acid were applied to the foliage of juvenile crop plants at 0.002, 0.009, 0.03, 0.14, and 0.56 kg/ha. Corn (Zea maysL.), oat (Avena sativaL.), wheat (Triticum aestivumL.), grain sorghum [Sorghum bicolor(L.) Moench], and kleingrass (Panicum coloratumL.) were generally more tolerant to the herbicides than were peanuts (Arachis hypogaeaL.), cotton (Gossypium hirsutumL.), cucumber (Cucumis sativusL.), and soybean (Glycine max[L.] Merr.). Triclopyr was usually more phytotoxic to corn, oat, grain sorghum, and kleingrass than either 2,4,5-T or 3,6-dichloropicolinic acid at 0.14 and 0.56 kg/ha, but few differences occurred among herbicides at lower rates. Kleingrass was not affected at any rate of 3,6-dichloropicolinic acid. Wheat tolerated most rates of all three herbicides. At 0.56 kg/ha, triclopyr and 3,6-dichloropicolinic acid caused greater injury to peanuts than did 2,4,5-T; whereas, 2,4,5-T and triclopyr were more damaging to cotton and cucumber than 3,6-dichloropicolinic acid. The three herbicides at 0.14 and 0.56 kg/ha killed soybeans. Soybean injury varied from none to severe at 0.002 to 0.03 kg/ha, depending upon species investigated, but many plants showed morphological symptoms typical of the auxin-type herbicides.


Author(s):  
O. J. Ilori ◽  
O. O. Otusanya

Tithonia. rotundifolia is an allelopathic weed that grows in association with cultivated crops in Nigeria. Allelopathy is a phenomenon of plant releasing allelochemicals into the environment that can inhibit or stimulate the growth of other plants and microorganisms. This study aimed to evaluate the effects of soil incorporated with the fresh shoots of T. rotundifolia on chlorophyll and protein accumulation of Vigna unguiculata L. Glycine max L., Zea mays L. and Sorghum bicolor L.  250 g of fresh shoots of T. rotundifolia were worked into each plot of 2 m2 dimension and the test crops were sown in the plots. Plots with no T. rotundifolia shoots were included as control plots. The experiment was performed in completely randomized block design (CRBD). The parameters measured were chlorophyll a, chlorophyll b total chlorophyll and protein content. The results showed a stimulation in chlorophyll contents in the test crops except inhibition in chl b and total chlorophyll in Zea mays L. Also, there was a stimulation of protein in Zea mays L. and Sorghum bicolor L. The study suggests that incorporation of shoots of Tithonia rotundifolia could imparts stimulatory allelopathic effects.


Weed Science ◽  
1986 ◽  
Vol 34 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Mark A. Peterson ◽  
W. Eugene Arnold

The response of corn (Zea maysL. ‘Sokota TS 46’), flax (Linum usitatissimumL. ‘Culbert 79’), grain sorghum [Sorghum bicolor(L.) Merr. ‘Sokota 466’), soybeans [Glycine max(L.) Merr. ‘Corsoy 79’], and sunflowers (Helianthus annuusL. ‘Sokata 4000’) to soil residues 12 and 24 months after application of 17, 34, and 68 g ai/ha chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl) amino] carbonyl] benzenesulfonamide} was determined at two locations, Redfield and Watertown, in eastern South Dakota. All crops at Redfield were injured significantly at 17 g/ha, 12 months after application as determined by plant dry weight and visual evaluations. Corn and sorghum were the most susceptible while flax was the least susceptible. Injury at Watertown was significantly less than at Redfield. Differences in carryover were related to a lower soil pH at Watertown.


Weed Science ◽  
1978 ◽  
Vol 26 (3) ◽  
pp. 252-254 ◽  
Author(s):  
D. F. Berard ◽  
D. P. Rainey ◽  
C. C. Lin

The uptake, translocation, and metabolism of the herbicide fluridone 1-methyl-3-phenyl-5-[3-(trifluoromethyl)phenyl]-4(1H)-pyridinone was studied in cotton (Gossypium hirsutum L. ‘Stoneville 213’) (fluridone tolerant) and corn (Zea mays L. ‘Migro 5040’), soybean [Glycine max (L.) Merr. ‘Calland’], and rice (Oryza sativa L. ‘Nato’) (fluridone susceptible). Uptake studies with 14C fluridone demonstrated that all species absorbed radioactivity with rice having the highest concentration. Equivalent concentrations of radioactivity were observed in corn, cotton, and soybean. Absorbed fluridone was retained primarily in the roots and the basal region of the stem of cotton, while in the herbicide susceptible species fluridone was readily translocated into the shoots. No appreciable metabolism of fluridone was observed in the roots or shoots of cotton and soybean. Tolerance of cotton to fluridone was primarily attributed to the limited translocation of the compound.


Weed Science ◽  
1969 ◽  
Vol 17 (4) ◽  
pp. 438-441 ◽  
Author(s):  
O. C. Burnside ◽  
G. A. Wicks ◽  
D. D. Warnes ◽  
B. R. Somerhalder ◽  
S. A. Weeks

Widely varying weed densities that had been desiccated by freezing did not reduce combine efficiency (gathering plus threshing losses) in harvesting corn (Zea mays L., var. Nebraska 508), sorghum [Sorghum bicolor (L.) Moench, var. RS 610], or soybeans (Glycine max L., var. Ford). Average combine gathering losses with corn, sorghum, and soybeans were 2, 7, and 16%, respectively. Average threshing losses with the combine were less than 1% with corn and sorghum and less than 3% with soybeans. The high gathering losses in this study were due partly to delayed combining to allow the weeds to be desiccated by freezing temperatures.


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