scholarly journals Respuesta in vitro de cepas de Trichoderma harzianum frente a FE3+, salinidad, pH y temperatura, con el fin de ser utilizadas en control biológico de Rhizoctonia solani y Fusarium solani en tomate.

2004 ◽  
Vol 19 ◽  
Author(s):  
P. Escobar ◽  
J. Montealegre ◽  
R. Herrera
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Fusarium Solani

scholarly journals Antagonismo de Trichoderma harzianum contra la fusariosis del garbanzo y su efecto biofertilizante

2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Baja California ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).

Antifungal activity of Trichoderma harzianum and T. koningiopsis against Fusarium solani in seed germination and vigor of Miahuateco chili seedlings

2021 ◽  
Vol 39 (2) ◽  
Author(s):  
Leydi Miguel-Ferrer ◽  
Omar Romero-Arenas ◽  
Petra Andrade-Hoyos ◽  
Primo Sánchez-Morales ◽  
José Antonio Rivera-Tapia ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Seed Germination ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Para Determinar ◽  
Santa Maria

El chile es la segunda hortaliza de mayor producción en México. El objetivo de la investigación fue evaluar la actividad antagónica <em>in vitro</em> e <em>in vivo</em> de <em>Trichoderma harzianum</em> (T-H4) y <em>T. koningiopsis</em> (T-K11) <em>versus Fusarium solani</em> (MX-MIC 798) en la germinación y establecimiento de plántula de chile Miahuateco. Se utilizó la técnica de cultivo dual para determinar el porcentaje de inhibición de crecimiento radial (PICR) de la cepa MX-MIC 798. Además, se analizó el porcentaje de germinación en semillas de chile Miahuateco en vivero, así como la mortalidad de plántulas y grado de severidad a los 40 días después del trasplante (ddt) en Santa María Tecomavaca, Oaxaca, a través de biocontrol y control químico (Mancozeb 80®). La cepa T-H4 presentó el nivel antagónico PICR más alto (53.3%) <em>in vitro</em> y clase II en la escala de Bell, asimismo obtuvo 82% de germinación en semillas de chile Miahuateco en vivero y 48% de mortalidad en campo; de manera que igualó al control químico y superó a <em>T. koningiopsis</em> T-K11. La actividad antifúngica de <em>Trichoderma</em> spp., ofrecen una alternativa para el biocontrol de la marchitez y necrosis en raíz del cultivo de chile Miahuateco causada por <em>F. solani</em> MX-MIC 798.

scholarly journals Testing Methods Affecting the Antagonistic Ability of  Pseudomonas Biocontrol Strains

2002 ◽  
Vol 51 (1-2) ◽  
pp. 107-114 ◽  
Author(s):  
I. Jevcsák ◽  
Bálint Oldal ◽  
L. Ködöböcz ◽  
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Fungal Pathogens ◽  
Culture Media ◽  
Colony Growth ◽  
Antagonistic Effect ◽  
Bacterial Strains ◽  
Testing Methods ◽  
Selection Of

The antagonistic effect of thirteen Pseudomonas aeruginosa and thirteen strains of other Pseudomonas species was studied on the soil-borne phytopathogenic Rhizoctonia solani and Fusarium solani fungi.  The inhibition of pathogen colony growth was tested with two different in vitro techniques using the same type of culture media. In case of the spread slant technique the antagonists induced a significantly stronger inhibition on the growth of pathogens than in case of spot transfer. Among the 26 investigated Pseudomonas strains, P. aeruginosa strains were generally more effective against the fungal pathogens. Rhizoctonia solani proved to be affected to a greater extent by the bacterial strains studied than the Fusarium solani representative. The possibility of in vitro strain selection of biocontrol microbes is being further discussed .

scholarly journals In vitro evaluation of fungicidal responses on the growth of pathogenic Rhizoctonia solani Kuhn, antagonistic binucleate Rhizoctonia and Trichoderma harzianum Rifai

1970 ◽  
Vol 39 (1) ◽  
pp. 107-110
Author(s):  
Md Maniruzzaman Khandaker ◽  
Md Khurshed Alam Bhuiyan ◽  
Abul Khair
Keyword(s):  
Boric Acid ◽  
Rhizoctonia Solani ◽  
Key Words ◽  
Trichoderma Harzianum ◽  
Stem Canker ◽  
Black Scurf ◽  
Binucleate Rhizoctonia ◽  
Crop Fields ◽  
Potato Plants

Two pathogenic isolates of Rhizoctonia solani Kuhn causing stem canker/black scurf disease of potato plants and four antagonist isolates, two of binucleate Rhizoctonia and two of Trichoderma harzianum Rifai were isolated from crop fields and evaluated in vitro for their fungicidal responses against eight fungicides. Vitavax was effective in inhibiting the growth of R. solani and binucleate Rhizoctonia but it did not inhibit the growth of T. harzianum at 100 ppm concentration. Terraclor Super X, Dithane M 45 and Boric acid are the fungicides which at 100 ppm concentration did not inhibit the growth of antagonist isolates of T. harzianum and binucleate Rhizoctonia but inhibited the growth of isolates of R. solani to some extent. The in vitro findings suggest that any one of these three fungicides along with antagonist isolates of binucleate Rhizoctonia and T. harzianum can be used as biocontrol agents to reduce soil borne inocula of R. solani. Key words: Rhizoctonia solani; Binucleate; Trichoderma harzianum; Fungicide DOI: 10.3329/bjb.v39i1.5534Bangladesh J. Bot. 39(1): 107-110, 2010 (June)

scholarly journals Trichoderma species as Biocontrol Agent against Soil Borne Fungal Pathogens

2017 ◽  
Vol 5 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Srijana Bastakoti ◽  
Shiva Belbase ◽  
Shrinkhala Manandhar ◽  
Charu Arjyal
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Biocontrol Agent ◽  
Sclerotium Rolfsii ◽  
Dual Culture ◽  
Trichoderma Species ◽  
Biocontrol Efficacy

Soil borne pathogenic fungi are of major concern in agriculture which significantly decreases the plant yield. Chemically controlled plant imposes environmental threats potentially dangerous to humans as well as other animals. Thus, application of biological methods in plant disease control is more effective alternative technique. This study was carried out to isolate Trichoderma species from soil sample and to assess its in vitro biocontrol efficacy against fungal pathogens viz. Sclerotium rolfsii, Sclerotionia sclerotiorum, Fusarium solani and Rhizoctonia solani. Biocontrol efficacy testing of isolates against different fungal pathogens was performed by dual culture technique.In this study, 5 different Trichoderma species were isolated from 26 various soil samples and were tested against four fungal soil-borne pathogens. Inhibition percentage of radial growth of Sclerotium rolfsii by three of the Trichoderma isolates was found to be 100%; about 62% and 68% of maximum inhibition was observed against Rhizoctonia solani and Fusarium solani respectively whereas Sclerotionia sclerotiorum was inhibited maximum up to 23%. This in vitro study revealed that although Trichoderma species plays an important role in controlling all type of soil borne fungal plant pathogens, however, isolates as biocontrol agent against Sclerotium rolfsii was found to be more efficient in comparison to other pathogens.Nepal Journal of Biotechnology. Dec. 2017 Vol. 5, No. 1: 39-49

scholarly journals In Vitro Nematophagous Activity of Predatory Fungi on Infective Nematodes Larval Stage of Strongyloidae Family

2017 ◽  
Vol 5 (3) ◽  
pp. 281-284
Author(s):  
Majid Zarrin ◽  
Mahmoud Rahdar ◽  
Farzad Poormohamadi ◽  
Ali Rezaei-Matehkolaei
Keyword(s):  
Larval Stage ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Nematophagous Fungi ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Duddingtonia Flagrans

AIM: The main goal of the present research conducted to assess the in vitro activity of the nematophagous fungi Duddingtonia flagrans, Fusarium solani, Verticillium chlamidosporium, and Trichoderma harzianum. MATERIAL AND METHODS: Four isolates of fungi including D. flagrans, F. solani , V. chlamidosporium and T. harzianum were used in this study. Horse faeces were used to provide the larvae stage of Strongyloidae family for the experiments.RESULTS: D. flagrans was the most effective fungus to reduce the population of the larval nematodes. D. flagrans was able to kill 100% of larvae after 14 days of incubation. The significant effect was seen after 7 days of incubation, therefore, the live larvae was decreased to 9, 11, 19 and 25 for D. flagrans, V. chlamidosporium, F. solani and T. harzianum, respectively. CONCLUSION: Our results illustrated that D. flagrans were most successful fungus for reducing the number of Strongylidae family larva stage from horse faeces. Follow D. flagrans, the live larvae was significantly reduced for V. chlamidosporium, F. solani and T. harzianum, respectively.

scholarly journals Sensibilidad in vitro de hongos fitopatógenos causantes de enfermedades en fresa a controladores biológicos y fungicidas, en el estado de Guanajuato, México

2019 ◽  
Vol 29 ◽  
pp. 1-11
Author(s):  
Luis Roberto Pérez Moreno ◽  
Luis Pérez-Moreno ◽  
Rafael Guzmán-Mendoza ◽  
Diana Sanzón-Gómez ◽  
José Roberto Belmonte-Vargas
Keyword(s):  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Factor B ◽  
Trichoderma Sp ◽  
Alternaria Sp ◽  
Fusarium Sp

Se evaluó la respuesta in vitro de un aislado de los hongos Alternaria sp., Fusarium sp., Rhizoctonia solani, a 16 agentes biológicos (AB), ocho fungicidas y un testigo. Se usó un diseño experimental completamente al azar con arreglo factorial. El factor A correspondió a los aislados del hongo y el factor B a los productos de control. La comparación de medias se realizó con la prueba de Tukey (p < 0.05). Se hizo la evaluación del crecimiento promedio radial micelial en centímetros (Cprm) cada 24 h durante 11 días. En cualquier caso, no se encontró efecto, solo o en interacción con los agentes de control. Dicloran, Tebuconazol y Cyprodinil-Fludioxonil inhibieron el crecimiento micelial de todos los aislados de los hongos con un promedio final de 1.0 Cprm, y en mayor proporción que los agentes biológicos. Los AB que propiciaron el menor crecimiento micelial de los aislados fueron: Trichoderma sp., Trichoderma harzianum, Microorganismos y Trichoderma viridae.

scholarly journals POTENCIAL ANTAGONÍSTICO DO ISOLADO BACTERIANO (BSV- 05) CONTRA OS PATÓGENOS RADICULARES DO FEIJOEIRO: Fusarium solani f. sp. phaseoli, Fusarium oxysporum f. sp. phaseoli, Macrophomina phaseolina e Rhizoctonia solani

2017 ◽  
Vol 14 (1) ◽  
pp. 59 ◽  
Author(s):  
Bruno Sérgio Vieira ◽  
Hyann Markos Pereira Vieira ◽  
Luciana Alves de Sousa ◽  
Karoline Damasceno Ribeiro de Mendonça
Keyword(s):  
Bacillus Subtilis ◽  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Fusarium Solani ◽  
Macrophomina Phaseolina ◽  
E 92

<p>Durante uma seleção de bactérias antagonistas realizada no Laboratório de Microbiologia e Fitopatologia da UFU, o isolado de <em>Bacillus subtilis</em>, BSV – 05 se destacou. Diante disso, objetivou-se no presente trabalho avaliar o potencial antagonístico <em>in vitro</em> deste isolado bacteriano contra quatro patógenos radiculares do feijoeiro: <em>Fusarium solani </em>f. sp. <em>phaseoli, Fusarium oxysporum</em> f. sp. <em>phaseoli, Macrophomina phaseolina </em>e <em>Rhizoctonia solani</em>. Foram utilizadas as seguintes metodologias <em>in vitro </em>versus os patógenos citados:<em> </em>cultivo pareado, influência da inoculação conjunta, produção de metabolitos voláteis e não voláteis. Os percentuais de inibição do isolado BSV – 05 sobre <em>M. phaseolina, R. solani, F. solani,</em> <em>F. oxysporum</em>, foram de 61,43%; 50,36%; 13,74% e 7,7%, respectivamente, para a metodologia do cultivo pareado. O contato direto da bactéria com os patógenos inibiu em 100% o crescimento micelial de <em>M. phaseolina, R. solani</em>; e para <em>F. solani </em>e <em>F. oxysporum</em>, foram observadas inibições de 90 e 92 %, respectivamente. Possíveis metabólitos secretados pelo isolado BSV 05 apresentaram níveis de inibição de 100% para <em>R. solani</em>. Para <em>M. phaseolina</em>, observou-se uma porcentagem de inibição de 80,26%, e 45,31% e 47,80% para <em>F. solani </em>e <em>F. oxysporum</em>, respectivamente. A porcentagem de inibição da germinação de conídios de <em>F. solani </em>e <em>F. oxysporum </em>e<em> M. phaseolina</em> num meio de cultura contendo substâncias metabolizadas por BSV – 05 foi de 87,70; 91,28% e 100%, respectivamente. O isolado bacteriano BSV-05 não apresentou produzir nenhum metabólito volátil.</p>

scholarly journals THE EFFECT OF CHITOSAN HYDROLYSATE ON THE FUNGI ALTERNARIA SOLANI, FUSARIUM SOLANI AND RHIZOCTONIA SOLANI IN VITRO

2021 ◽  
Vol 26 ◽  
pp. 200-209
Author(s):  
Balzhima Ts. Shagdarova ◽  
◽  
Natalia V. Karpova ◽  
Alla V. Il’ina ◽  
Valery P. Varlamov
Keyword(s):  
Molecular Weight ◽  
Nitric Acid ◽  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Radial Growth ◽  
Alternaria Solani ◽  
Potato Dextrose Agar ◽  
Deacetylation Degree ◽  
Growth Experiments

Chitosan hydrolysate was obtained using nitric acid; the prevailing fraction had a molecular weight of 30 kDa and a deacetylation degree of 95%. The effect of chitosan hydrolysate when added to potato dextrose agar (PDA) in different concentrations (0.5, 1, 1.5, 2, 4, 6 and 8 mg/mL) was studied on the growth of the fungi Alternaria solani Sorauer, Fusarium solani (Mart.) Sacc. and Rhizoctonia solani J.G. Kühn. A. solani was the most sensitive to the addition of chitosan hydrolysate to PDA in radial growth experiments. On days 3 and 7 of incubation, the antifungal activity of the phytopathogen growth was 69%-92% and 69%-88%, respectively, in the concentration range of 0.5-2 mg/ml.

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