scholarly journals ANALISIS KADAR KAFEIN DALAM MINUMAN KOPI KHOP ACEH DENGAN METODE SPEKTROSKOPIK

2019 ◽  
Vol 6 (2) ◽  
pp. 141
Author(s):  
Sabarni Sabarni ◽  
Nurhayati Nurhayati
Keyword(s):  
Liquid Extraction ◽  
Hot Water ◽  
Special Study ◽  
Dry Extract ◽  
Liquid Liquid Extraction ◽  
Powder Diameter ◽  
Coffee Powder ◽  
The City ◽  
Coffee Shops ◽  
Layer Chromatography

In addition of being dubbed as the city of "Serambi Mekah", Aceh is also famous as the city of "Thousand Coffee Shops". One of coffee dish that attracts consumers' is khop coffee (read: kopi khop) because it is served with a glass dish upside down. The khop coffee, a typical coffee drink from Meulaboh, West Aceh Regency, is served by reversing the glass that has been filled with coarse coffee powder on top of the pan. Therefore, it is deemed necessary to conduct a special study on the analysis of unique taste-determining compounds in khop coffee to elevate the local wisdom of the Acehnese to the scientific region. The aim of this study was to determine the concentration of caffeine compounds of khop coffee due to the modification of coffee powder diameter by using the thin layer chromatography (TLC) method and UV-Vis spectrophotometry. This study used four types of samples with diameter variations coffee, namely original, fine, medium, and coarse. The four samples were extracted with boiling hot water according to the same conditions when the bartender served it (90 °C) followed by liquid-liquid extraction using chloroform solvent. Dry extract was analyzed by using TLC followed by a UV-Vis spectrometer. The results showed that the concentrations of original, fine, medium, and coarse coffee were respectively 57.85%; 17.61%; 1.29%; and 0.83%.

scholarly journals Analisis Kadar Kafein dalam Kopi Bubuk Lokal yang Beredar di Kota Palu

2018 ◽  
Vol 7 (4) ◽  
pp. 189
Author(s):  
I Nyoman Suwiyarsa ◽  
Siti Nuryanti ◽  
Baharuddin Hamzah
Keyword(s):  
Quantitative Analysis ◽  
Qualitative Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Percent Level ◽  
Caffeine Content ◽  
Coffee Powder ◽  
The City ◽  
Layer Chromatography ◽  
Caffeine Level

Analysis of caffeine level in local coffee powder that circulates in Palu city have been done. The purpose of this study was to determine the level of caffeine in local coffee powder, which is engaged in several markets in the city of Palu. Levels of caffeine in the study will be compared with the standard caffeine content according to the SNI 01-3542-2004, i.e. 0.45-2% w/w. The method used for qualitative analysis was the thin layer chromatography and for quantitative analysis was using UV-Vis spectrophotometry. The results of qualitative analysis of six local coffee powders A, B, C, D, E, and F showed each a Rf value between 0.32 to 0.40. Levels of caffeine (per 1 g of coffee powder) quantitatively ranging from brand samples A to F were 0.83, 2.06, 1.60, 2.63, 1.29, and 1.72%, respectively, percent level of caffeine of six samples of local powdered coffee, samples A, C, E and F in terms of SNI 01-3542-2004 were between 0.45-2.00% (w/w), while sample B and sample D exceeds 2% were 2.06% and 2.63%.

scholarly journals Investigação de adulterantes em amostras de cocaína apreendidas na região de Araçatuba no período de 2014 a 2015

2019 ◽  
Vol 8 (1) ◽  
pp. 54-61
Author(s):  
Sra. Giancotti Ferreira ◽  
Bruno Spinosa de Martinis ◽  
Gleici Da silva de Castro Perdoná ◽  
Fabiana Spineti dos Santos
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Methyl Ester ◽  
Liquid Extraction ◽  
Chemical Profile ◽  
Liquid Liquid Extraction ◽  
Ketamine Hydrochloride ◽  
Mass Spectrometry Detector ◽  
The City ◽  
Drug Studies

A maior parte da cocaína traficada nas ruas apresenta substâncias produto dos processos de obtenção, diluentes adicionados para aumento do lucro, e adulterantes, adicionados para mimetizar ou potencializar os efeitos da droga. Estudos sobre o perfil químico de cocaína apreendida vem sendo explorados em vários países, e com destaque, no Brasil, objetivando não só conhecer o perfil da droga traficada, mas também relacionar características químicas com origens geográficas. Este trabalho investigou os componentes de 92 amostras de cocaína apreendidas na região de Araçatuba, no período de 2014 a 2015 empregando o método de extração líquido-líquido e análise por Cromatografia em fase Gasosa acoplada a detector por Espectrometria de Massas, em que foram detectados os adulterantes cafeína, lidocaína, fenacetina, levamisol, carisoprodol, aminopirina, benzocaína metotrimeprazina e cloridrato de cetamina. Também foram identificados os alcaloides éster de metilecgonidina, cinamoilcocaínas e norcocaína. A maior parte das amostras estava adulterada e apresentou o éster de metilecgonidina, substância formada a partir da degradação térmica da cocaína. Foram elaborados mapas georreferenciados baseados nos sítios das apreensões das amostras, em que se pode visualizar três principais núcleos de densidade de apreensões, localizados na cidade de Araçatuba.AbstractMost of street cocaine has substances derived from the processes of production, diluents added to increase profits, and adulterants added to mimic or potentiate the effects of the drug. Studies on the chemical profile and geographical origins of the seized cocaine have been explored in several countries, including Brazil. This work investigated the compounds present in 92 cocaine samples seized in the region of Araçatuba city, from 2014 to 2015 using the method of liquid-liquid extraction and analysis by Gas Chromatography coupled to Mass Spectrometry detector, in which were detected the adulterants caffeine, lidocaine, phenacetin, levamisole, carisoprodol, aminopyrine, benzocaine metotrimeprazine and ketamine hydrochloride. The alkaloids ecgonidine methyl ester, cinnamoylcocaines and norcocaine were also identified. Most of the samples were adulterated and presented ecgonidine methyl ester, a substance formed from the thermal degradation of cocaine. Georeferenced maps were made based on the sample seizure sites, showing the presence of three main hotspots in the city of Araçatuba.           

scholarly journals Qualitative TLC determination of some polycyclic aromatic hydrocarbons in sugar-beet

2005 ◽  
Vol 70 (10) ◽  
pp. 1237-1242 ◽  
Author(s):  
Eva Loncar ◽  
Ljiljana Kolarov ◽  
Radomir Malbasa ◽  
Biljana Skrbic
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Sugar Beet ◽  
Silica Gel ◽  
Aromatic Hydrocarbons ◽  
Liquid Extraction ◽  
Polynuclear Aromatic Hydrocarbons ◽  
Liquid Liquid Extraction ◽  
Polycyclic Aromatic ◽  
Layer Chromatography

The presence of polycyclic or polynuclear aromatic hydrocarbons (PAHs) were investigated in sugar-beet from a local sugar factory in the district of Vojvodina. The sugar-beet was cultivated on areas near roads with intensive traffic. The procedure for the preparation and determination of these compounds included saponification of the sample, several liquid?liquid extraction systems and a silica gel column clean-up. The purified sample solution was analysed by thin layer chromatography (TLC) on silica gel with cyclohexane as the developing solvent. Benzo(b)fluoranthene and benzo(a)anthracene and/or benzo(a)pyrene were detected at concentrations greater than the allowed limits in food.

scholarly journals Chemical and Toxicological Diagnosis of Acute Poisonings with Phenazepam

2019 ◽  
Vol 7 (4) ◽  
pp. 319-324
Author(s):  
M. V. Belova ◽  
E. A. Klyuyev ◽  
E. S. Melnikov ◽  
D. M. Yeliseyeva
Keyword(s):  
High Performance ◽  
Acute Poisoning ◽  
Liquid Extraction ◽  
High Tech ◽  
Mass Selective Detector ◽  
Liquid Liquid Extraction ◽  
Selective Detector ◽  
Suicidal Attempts ◽  
Confirmatory Analysis ◽  
Layer Chromatography

Background. The relative availability of Phenazepam makes it a frequent cause of overdose, suicide and non-medical use. At the same time, it remains insufficiently studied in chemical and toxicological terms.The aim of study. to create an accessible, rapid method for detecting Phenazepam in biological matrices of patients with acute poisoning.Materials and methods. We used thin-layer chromatography (TLC), gas chromatography with a mass selective detector (GC-MS), high performance liquid chromatography with a tandem mass-selective detector (LC-MS/MS) and immunochromatographic analysis (ICA). The preparation of samples of intact urine with the addition of standard solutions of Phenazepam and real urine samples of patients with acute poisoning with Phenazepam was carried out using liquid-liquid extraction or precipitation of related components of the sample with acetonitrile. Hydrolysis and derivatization were also added in GC-MS analysis.Results. The analysis of statistics of the Department of Acute Poisonings of the N.V. Sklifosovsky Research Institute for Emergency Medicine in 2014-2016 showed that Phenazepam poisonings averaged 9.2% of the total number of admissions and mainly occurred as suicidal attempts. A technique has been developed for the detection of Phenazepam by TLC, which gives more objective results than ICA. For confirmatory analysis, it is advisable to use LC-MS/MS method for the native substance and GC-MS for the products of hydrolysis after derivatization. Compared to confirmatory methods, the developed TLC-screening technique is expressive, does not require the use of expensive high-tech equipment, difficult sample preparation, and makes it possible to reliably detect toxic and lethal concentrations of Phenazepam.

Sign in / Sign up

Export Citation Format

Share Document