Detection & characterization of PHB (polyhydroxybutyrate) Producers halophilic bacteria isolated from marine water sample of Valsad District

2017 ◽  
Vol 8 (3) ◽  
Author(s):  
NIDHI PATEL ◽  
PAYAL PATEL ◽  
REENA DESAI
Keyword(s):  
Water Sample ◽  
Halophilic Bacteria ◽  
Marine Water

scholarly journals Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern

2021 ◽  
Vol 38 (4) ◽  
pp. 527-531
Author(s):  
Pınar Çağlayan
Keyword(s):  
Water Sample ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Negative Results ◽  
Gram Staining ◽  
Yellow Orange ◽  
C 50 ◽  
Indole Production

In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimally 35°C), pH 6-8 (optimally pH 7) and 3-20% NaCl (optimally 10% NaCl). It was not able to grow at 4°C, 10°C, 50°C, salt-free, 0.5%, 25%, %30 total salt, pH 4-5, and pH 9-12. Glucose, ribose, fructose, sucrose, maltose were used by the test isolate as carbon sources. Different amino acids found in the structure of animal hide such as L-lysine, L-arginine, L-cysteine, L-alanine, L-tyrosine, L-histidine were also utilized by the bacterium. During the salt production process, this bacterium may contaminate the salt which is used in the food and leather industries. The activities of harmful moderately halophilic bacteria should be prevented by effective antimicrobial applications.

Preparation and Characterization of Herbicide Mecoprop Imprinted Polymer and Its Application as a Selective Sorbent in Water Sample

2018 ◽  
Vol 101 (5) ◽  
pp. 657-663
Author(s):  
Elaheh Mansouri ◽  
Mahboobe Sarabi-Jamab ◽  
Behrouz Ghorani ◽  
Seyed Ahmad Mohajeri
Keyword(s):  
Water Sample ◽  
Imprinted Polymer ◽  
Selective Sorbent

scholarly journals Isolation of a Vibrio parahaemolyticus pandemic strain from a marine water sample obtained in the northern Adriatic

2008 ◽  
Vol 13 (11) ◽  
pp. 5-6 ◽  
Author(s):  
G Caburlotto ◽  
V Ghidini ◽  
M Gennari ◽  
M C Tafi ◽  
M M Lleo
Keyword(s):  
Water Sample ◽  
Vibrio Parahaemolyticus ◽  
Marine Water ◽  
Northern Adriatic ◽  
Pandemic Strain ◽  
Binary File

Binary file ES_Abstracts_Final_ECDC.txt matches

scholarly journals Insilico Analysis of PHB from Halophiles for potential Bio-medical applications

2019 ◽  
pp. 211-218
Author(s):  
Vinod. P S ◽  
Neha Guttikonda ◽  
Snehal. M. Mathe ◽  
M B Sulochana
Keyword(s):  
Carbon Dioxide ◽  
Nuclear Magnetic Resonance ◽  
Drug Carrier ◽  
Halophilic Bacteria ◽  
Docking Studies ◽  
Medical Applications ◽  
Hydroxybutyric Acid ◽  
Lipinski’S Rule ◽  
Toxicity Analysis

Polyhydroxybutyrate (PHB) is an important biopolymer accumulated by halophilic organisms. PHA is a family of polyesters is accumulated as granules in the cell of bacteria. Polyhydroxybutyrate (PHB) can be used as an alternative polymer to polylactide-glycolides for drug carrier production. It is a linear homopolymer biosynthesized by various strains of bacteria by condensation of D (-)-B-hydroxybutyric acid and used as an energy and carbon source. PHB can be obtained by extraction from bacteria or by chemical synthesis. To be suitable as drug carrier the PHB (polymer) has to be biocompatible, biodegradable in certain applications, and nontoxic. PHB seems to be biocompatible and biodegrades readily to carbon dioxide in bacteria; however, in humans, the reports are few and contradictory. The PHB was extracted from halophilic bacteria. The structural characterization of PHB was done by using NMR (nuclear magnetic resonance). To genenerate SMILES the structure were drawn in MarvinSketch. The PHB were screened based on the Lipinski’s rule of 5. The PHB molecule was subjected to the toxicity analysis and those that passed the toxicity test analyzed for docking studies.

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