scholarly journals Characterization of Planococcus dechangensis isolated from a water sample of Çamaltı Saltern

2021 ◽  
Vol 38 (4) ◽  
pp. 527-531
Author(s):  
Pınar Çağlayan
Keyword(s):  
Water Sample ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Rrna Gene ◽  
Negative Results ◽  
Gram Staining ◽  
Yellow Orange ◽  
C 50 ◽  
Indole Production

In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional and molecular methods. 16S rRNA gene sequence analyses showed that the strain MHDS3 belonged to Planococcus dechangensis species. It gave a positive result in the Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative and the colony pigmentation was yellow-orange. It showed negative results for citrate utilization, indole production from tryptophane, Voges-Proskauer and methyl red. This isolate was able to grow at 10-45°C (optimally 35°C), pH 6-8 (optimally pH 7) and 3-20% NaCl (optimally 10% NaCl). It was not able to grow at 4°C, 10°C, 50°C, salt-free, 0.5%, 25%, %30 total salt, pH 4-5, and pH 9-12. Glucose, ribose, fructose, sucrose, maltose were used by the test isolate as carbon sources. Different amino acids found in the structure of animal hide such as L-lysine, L-arginine, L-cysteine, L-alanine, L-tyrosine, L-histidine were also utilized by the bacterium. During the salt production process, this bacterium may contaminate the salt which is used in the food and leather industries. The activities of harmful moderately halophilic bacteria should be prevented by effective antimicrobial applications.

scholarly journals A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia

2021 ◽  
Vol 22 (9) ◽  
Author(s):  
Sutami SUTAMI ◽  
Purwanto PURWANTO ◽  
Retno Rosariastuti
Keyword(s):  
Gold Mining ◽  
Rrna Gene ◽  
Salt Tolerant ◽  
Indigenous Bacteria ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Query Coverage ◽  
Physiological Tests ◽  
Indole Production

Abstract. Sutami, Purwanto, Rosariastuti R. 2021. A salt tolerant Sphingosinicella microcystinivorans A3 isolated from soil contaminated with mercury in traditional gold mining of Jendi Village, Wonogiri District, Indonesia. Biodiversitas 22: 3785-3791. Isolation and characterization of indigenous bacteria from the soil of traditional gold mining contaminated with mercury is the first step in a series of research to explore and utilize indigenous bacteria in Jendi's area. This study was aimed to determine the characteristics and identity of bacterial isolates from soil of traditional gold mining in Jendi Village, Wonogiri contaminated by mercury. The methods used in this study included bacterial isolation, media preparation, phenotypic identification including; morphological and physiological tests and genotyping tests. The results showed that the bacterial isolate A3 grew optimally in media with the addition of 10% NaCl, at a temperature of 27°C, and pH 9. There were negative reactions to the observations of gram staining, acid production from glucose, indole production, catalase and urease, and positive reactions to oxidation. A neighbor-joining phylogenetic tree based on the 16S rRNA gene sequence showed that the A3 strain was closely related to Sphingosinicella microcystinivorans strain Y2T (JCM 13185T) with 100% Query coverage and a maximum identity of 99.56%.

IDENTIFICATION AND CHARACTERIZATION OF PIGMENTED BACTERIA ISOLATED FROM MALAYSIAN SEAWATER

2019 ◽  
Vol 7 (4) ◽  
pp. 01-08
Author(s):  
Nur Afifah Mursyida Zaujan ◽  
Mohamad Zohdi Othman ◽  
Fatin Najihah Mohd Lutfi ◽  
Kamarul Rahim Kamarudin ◽  
Hanina Mohd Noor ◽  
...  
Keyword(s):  
Human Health ◽  
Pcr Amplification ◽  
Fluorescent Light ◽  
Rrna Gene ◽  
Yellow Orange ◽  
Exiguobacterium Profundum ◽  
The Stability ◽  
Antimicrobial Metabolites ◽  
Pigmented Bacteria

Purpose of study: Bacteria can naturally produce pigments that can be useful for various applications as they possess antimicrobial metabolites among other numerous benefits towards the human health. This study was carried out to identify the species of marine bacterial isolates PMA, PM3C1 and PM5C1 exhibiting yellow, orange and green colors respectively. Methodology: The current study is using Polymerase Chain Reaction (PCR) amplification and sequence analysis of their 16S rRNA gene. The stability of pigments extracted from the bacterial samples was also analyzed against different temperature and light conditions. Main Findings: Sequence alignment using BLAST revealed that the yellow, orange, and green-pigmented bacteria have 84% similarity with Staphylococcus aureus, 85% similarity with Exiguobacterium profundum and 95% similarity with Pseudomonas aeruginosa respectively. The green pigment showed major changes in color following exposure to sunlight and fluorescent light, and when incubated at 24°C and 50°C. Exposure to direct sunlight also results in the reduction of color for the yellow and orange extracts, while no effect was observed for both pigments under fluorescent light. Incubation at 50°C results in the reduction of the orange color, while the yellow pigment was observed to be unaffected suggesting its stability at high temperature. Implications: Natural pigments production can provide many advantages including reduction of pollution generation, ease of disposal and other benefits to the human health.

scholarly journals Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4168-4172 ◽  
Author(s):  
Huimin Wang ◽  
Tingsheng Xiang ◽  
Yanwei Wang ◽  
Jinlong Song ◽  
Yi Zhai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Water Sample ◽  
Type Species ◽  
Contaminated Water ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Pr China

A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410T, was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153T and Microbacterium murale DSM 22178T with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA–DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T m method. Based on its phenotypic and genotypic properties, strain LAM0410T represents a novel species of the genus Microbacterium , for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410T ( = ACCC 00719T = JCM 19612T).

Characterization of halophilic bacteria from environmental samples from the brackish water of Pulicat Lake, India

Biologia ◽  
2011 ◽  
Vol 66 (5) ◽  
Author(s):  
Harmesh Sahay ◽  
Surendra Singh ◽  
Rajeev Kaushik ◽  
Anil Saxena ◽  
Dilip Arora
Keyword(s):  
16S Rdna ◽  
Brackish Water ◽  
Phylogenetic Analyses ◽  
Halophilic Bacteria ◽  
Phenotypic Characterization ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Gram Positive Bacteria ◽  
Nutrient Agar Medium

AbstractCulture dependent phenotypic characterization and 16S rDNA based phylogenetic analyses were applied to study the aerobic halophilic bacterial population present in the Pulicat brackish-water Lake of India. Five different media were employed for isolation of bacteria. A total of 198 morphotypes were recovered, purified and screened for salt tolerance in nutrient agar medium amended with 5–25% NaCl. Based on 16S rDNA restriction fragment length polymorphism analysis with three restriction endonucleases, 51 isolates tolerant to 5% or more NaCl were grouped into 29 clusters. Phylogenetic analysis using 16S rRNA gene sequences revealed that 29 strains could further be allocated into two clades: 19 to Firmicutes and 10 to γ-Proteobacteria. Firmicutes included low G+C Gram-positive bacteria related to family Bacillaceae, which included five genera Bacillus, Virgibacillus, Rummelibacillus, Alkalibacillus and Halobacillus. Another genera included in Firmicutes was Salimicrobium halophilum. In the γ-Proteobacteria group, all the isolates belonged to one genus Halomonas, represented by six different species Halomonas salina, H. shengliensis, H. salifodinae, H. pacifica, H. aquamarina and H. halophila. Most of the isolates exhibited cellulase, xylanase, amylase and protease activities.

scholarly journals Flavobacterium daemonensis sp. nov., isolated from Daemo Mountain soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 983-989 ◽  
Author(s):  
Hien T. T. Ngo ◽  
MooChang Kook ◽  
Tae-Hoo Yi
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Sequence Comparisons ◽  
Content Type ◽  
Phylogenetic Characterization ◽  
Link Type ◽  
Gram Staining ◽  
Bright Yellow

A Gram-staining-negative, catalase- and oxidase-positive, obligately aerobic, motile by gliding, bright yellow and rod-shaped bacterial strain was isolated from soil of Daemo Mountain (Daemosan) in Seoul, Republic of Korea. Its taxonomic position was investigated by using a polyphasic study. On the basis of 16S rRNA gene sequence comparisons, strain THG–DJ7T was found to be most closely related to Flavobacterium denitrificans ED5T (97.9 % sequence similarity), Flavobacterium ginsenosidimutans THG 01T (97.6 %), Flavobacterium kyungheensis THG-107T (97.5 %), Flavobacterium anhuiense D3T (97.1 %) and Flavobacterium ginsengisoli DCY54T (97.0 %). The DNA–DNA relatedness between strain THG–DJ7T and its phylogenetically closest neighbours was below 40.0 %. The G+C content of the genomic DNA was determined to be 32.6 mol%. The only isoprenoid quinone detected in strain THG–DJ7T was menaquinone-6 (MK-6). The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were found to be phosphatidylethanolamine, phosphatidyldimethylethanolamine, an unidentified aminophosphoglycolipid, phosphatidylserine and an unidentified lipid. The major fatty acids were identified as iso-C15 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c). These data support the affiliation of strain THG–DJ7T to the genus Flavobacterium . We report the phenotypic, genotypic and phylogenetic characterization of strain THG–DJ7T. Based on the findings presented, a novel species of the genus Flavobacterium , Flavobacterium daemonensis sp. nov. is proposed, with THG–DJ7T ( = KACC 17651T = JCM 19455T) as the type strain.

scholarly journals Production and Characterization of Polyhydroxybutarate from Halophilic Bacteria

2018 ◽  
pp. 44-52
Author(s):  
Vinod P. S ◽  
Deepali S. Kulkarni ◽  
M. B. Sulochana
Keyword(s):  
Sodium Chloride ◽  
Halophilic Bacteria ◽  
Carbon Sources ◽  
Biodegradable Plastics ◽  
Biodegradable Plastic ◽  
Biotechnological Application ◽  
Halophilic Microorganisms ◽  
Phb Production ◽  
Living Organisms

Halophilic microorganisms thrive at elevated concentrations of sodium chloride up to saturation and are capable of growing on a wide variety of carbon sources. Hence, the biotechnological application of these microorganisms can cover many aspects one of these is bioplastic production. Biodegradable plastics are plastics that are decomposed by the action of living organisms using bacteria. Biodegradable plastic is a renewable biopolymers synthesized in bacteria having similar characteristics of plastics produced from petroleum character. In the following work preisolated halophilic strains were screened for PHB production. The positive strains were subjected to PHB production. Estimation of PHB was done by using UV spectrophotometer and FTIR. Highest PHB producing strain was further used for optimization of different parameters for PHB production.

Morphological and Molecular Characterization of Lactic acid Bacteria from intestine of Freshwater Fishes and Their Possible Role in Probiotic Feeding in Aquaculture

2015 ◽  
Vol 5 (3) ◽  
pp. 101-106
Author(s):  
Dinesh Kumar Kumawat ◽  
Nadim Chishty
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Salt Tolerance ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Micrococcus Luteus ◽  
Food Preservation ◽  
Rrna Gene ◽  
Gram Staining

  Lactic acid bacteria (LAB) are a group of gram positive, non spore forming, cocci or rod shaped, catalase negative organisms. Generally they are known as "GRAS" (Generally Recognized As Safe) organisms. In the present study, an attempt was made to isolate lactic acid bacteria from gut of two freshwa-ter fishes in Udaipur region to obtain various efficient LAB strains for indus-trial applications. A total of 12 bacterial isolates were isolated from different fishes caught from Pichola lake, Udaipur on MRS agar at 37oC. All the isolates were tested for catalase activity, colony morphology and gram staining. Further these isolates were checked for technological properties (antibacterial activity, antibiotic susceptibility and bile salt tolerance). Based on technologi-cal properties results, two isolate (F-4 & F-11) have been tentatively selected for use as probiotic cultures in the aquaculture industries. They have strong antibacterial activity against various pathogens viz. Staphylococcus aureus (ATCC 9144), Micrococcus luteus (ATCC 10240), Listeria monocytogens (MTCC 1143) and have bile salt tolerance limit up to 0.7%. These are the main features for selection of probiotic candidates. To differentiate the same species molecular typing was attempted, 16S rRNA gene amplification was done using specific 16S rDNA primers and amplified gene products were di-gested with different restriction endonuclease enzymes viz EcoRI and HindIII. A total of 2 different groups out of 12 isolates were obtained. This finding reveals that isolated LAB are useful for aquaculture and also used in food preservation.

scholarly journals From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov.

2014 ◽  
Vol 80 (13) ◽  
pp. 3850-3857 ◽  
Author(s):  
María José León ◽  
Ana B. Fernández ◽  
Rohit Ghai ◽  
Cristina Sánchez-Porro ◽  
Francisco Rodriguez-Valera ◽  
...  
Keyword(s):  
Pure Culture ◽  
Culture Media ◽  
Halophilic Bacteria ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Content Type ◽  
Moderately Halophilic ◽  
Isolation And Characterization ◽  
Moderately Halophilic Bacterium

ABSTRACTRecent metagenomic studies on saltern ponds with intermediate salinities have determined that their microbial communities are dominated by bothEuryarchaeotaand halophilic bacteria, with a gammaproteobacterium closely related to the generaAlkalilimnicolaandArhodomonasbeing one of the most predominant microorganisms, making up to 15% of the total prokaryotic population. Here we used several strategies and culture media in order to isolate this organism in pure culture. We report the isolation and taxonomic characterization of this new, never before cultured microorganism, designated M19-40T, isolated from a saltern located in Isla Cristina, Spain, using a medium with a mixture of 15% salts, yeast extract, and pyruvic acid as the carbon source. Morphologically small curved cells (young cultures) with a tendency to form long spiral cells in older cultures were observed in pure cultures. The organism is a Gram-negative, nonmotile bacterium that is strictly aerobic, non-endospore forming, heterotrophic, and moderately halophilic, and it is able to grow at 10 to 25% (wt/vol) NaCl, with optimal growth occurring at 15% (wt/vol) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that strain M19-40Thas a low similarity with other previously described bacteria and shows the closest phylogenetic similarity with species of the generaAlkalilimnicola(94.9 to 94.5%),Alkalispirillum(94.3%), andArhodomonas(93.9%) within the familyEctothiorhodospiraceae. The phenotypic, genotypic, and chemotaxonomic features of this new bacterium showed that it constitutes a new genus and species, for which the nameSpiribacter salinusgen. nov., sp. nov., is proposed, with strain M19-40T(= CECT 8282T= IBRC-M 10768T= LMG 27464T) being the type strain.

Chryseobacterium angstadtii sp. nov., isolated from a newt tank

2013 ◽  
Vol 63 (Pt_12) ◽  
pp. 4777-4783 ◽  
Author(s):  
Karen E. Kirk ◽  
Jessica A. Hoffman ◽  
Katherine A. Smith ◽  
Brittane L. Strahan ◽  
Kevin C. Failor ◽  
...  
Keyword(s):  
Type Species ◽  
Distinct Species ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Protein Coding ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Yellow Orange ◽  
Polyphasic Characterization

As part of an undergraduate microbiology course, a yellow–orange-pigmented, Gram-staining negative, rod-shaped, non-motile bacterial strain was isolated from a glass tank housing several red-spotted newts (Notophthalmus viridescens). The sequence of the 16S rRNA gene of this strain, designated KMT, was 97.4–98.0 % similar to those of the type strains of Chryseobacterium luteum , C. shigense and C. vrystaatense , while the similarity levels for protein-coding genes were less than 94.7 % for rpoB, less than 92.1 % for groEL and less than 87.1 % for gyrB. These values are lower than for many other established distinct species. Polyphasic characterization and comparison to these relatives revealed that strain KMT was similar to other Chryseobacterium strains in that it contained MK-6 as its major respiratory quinone and phosphatidylethanolamine as the most abundant polar lipid, produced flexirubin-type pigments, oxidase and catalase and primarily contained the fatty acids iso-C15 : 0, iso-C17 : 1ω9c, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c). Based on the results of this study, strain KMT represents a novel species, for which the name Chryseobacterium angstadtii sp. nov. is proposed. The type strain is KMT ( = ATCC BAA-2160T = NRRL B-59516T = KCTC 23297T).

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