scholarly journals Determination of Antioxidants by Four Different Methods in Medicinally Important Plant Ebenus Stellata of Balochistan

2020 ◽  
Vol 23 (4) ◽  
pp. 13-18
Author(s):  
Aminullah Mandokhail ◽  
◽  
Samiullah Samiullah ◽  
Naqeebullah Khan ◽  
Abdul Hakeem Tareen ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Acid Concentration ◽  
Frap Assay ◽  
Antioxidant Power ◽  
North West ◽  
The North ◽  
Total Antioxidant ◽  
High Antioxidant Activity

The quantification of a plant, which is vital in medication bearing antioxidant activity of the North West area of the province Balochistan Ebenus Stellata family Fabaceae is explained in this paper. The plant composition was determined chemically through well-known four analytical tests and sensitive EMR operating techniques. The crude extract was obtained from the plant by using methanol, and the antioxidant activity of that extract was measured. Additionally, with the help of spectrophotometer, antioxidant potentials were determined through the DPPH (2, 2-Diphenyl-1-Picrylhydrazyl) assay, Ferric ion (Fe+3) reducing antioxidant power assay, Ferric (Fe+3) reducing antioxidant potential (FRAP) assay, and total antioxidant capacity (TAC) of the phosphomolybdenum assay. The potency of the DPPH assay ofEbenus Stellatawas 179 mg/g ofascorbic acid concentration. Moreover, the FRAP and TAC values were 9.2mg and 35 mg of ascorbic acid/g ofEbenus stellate extract, respectively. Furthermore, RP denotes 2.6 mg/g of ascorbic acid concentration. The concerned plant showed high antioxidant activity.

A Study on Synthesis and Antioxidant Activity Comparison of Novel Stilbenebenzamide Compounds

2019 ◽  
Vol 15 ◽  
Author(s):  
Aleksandra Kładna ◽  
Paweł Berczyński ◽  
Oya Bozdağ Dündar ◽  
Irena Kruk ◽  
Beyza Torun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Wide Spectrum ◽  
Antioxidant Properties ◽  
Butylated Hydroxytoluene ◽  
Hydroxyl Group ◽  
Antioxidant Power ◽  
Total Antioxidant

Background: Stilbene phytalexis (1,2-diphenyloethylen) and benzamide are beneficial for human health. To increase the stilbene ring activity, a new series of its derivatives containing benzamide structure was synthesized and evaluated for their in vitro antioxidant power. Methods: 1H nuclear magnetic resonance, mass spectroscopy, and chromatographic analyses were used to confirm the successful synthesis. The antioxidant properties were determined by the elimination of , HO , DPPH , ABTS+ radicals, total antioxidant status (TAS) and the ferric reducing antioxidant activities (TAC) measurements. Results: Stilbenebenzamide compounds showed a wide spectrum of antioxidant ability, however their total antioxidant power was weaker than those of butylated hydroxytoluene (BHT), ascorbic acid, and resveratrol. The highest antiradical activity towards and HO was shown by the compounds with structures containing amine group (SBEBA, SBA) ( : 37.7 – 38.0% and 40.8 – 43.5%, HO : 29.8%, 28.7% inhibition, respectively) at1.25 mM concentration. The antiradical power of SBEBA (0.29) in DPPH assay was lower than those of resveratrol (1.83), ascorbic acid (3.63) and BHT (4.09). The TAS values of the synthesized compounds ranged from 152.9±5.3 to 240.2±6.7µM trolox equivalent/gram (TE/g) and were much lower than those of BHT (1304±43.0), reservatrol (1360±29.0) and ascorbic acid (2782±39.7) µM TE/g. Similarly, the TAC values ranging from 29.7±0.9 to 41.5±1.6 µM TE were weaker than that of resveratrol (239.2 ±6.7 µM TE/g). Conclusion: The results suggest that the presence of hydroxyl group in stilbene ring should be considered in further design of stilbenebenzamide compounds to enhance their antioxidant activity.

scholarly journals Polyphenols and antioxidant activity in pseudocereals and their products

10.5219/1341 ◽  
2020 ◽  
Vol 14 ◽  
pp. 365-370
Author(s):  
Soňa Škrovánková ◽  
Dagmar Válková ◽  
Jiří­ Mlček
Keyword(s):  
Antioxidant Activity ◽  
Functional Foods ◽  
The Body ◽  
Gluten Free ◽  
Positive Health ◽  
High Positive Correlation ◽  
Spectrophotometric Methods ◽  
Total Antioxidant ◽  
High Antioxidant Activity

Pseudocereals are important as gluten-free crops that could be utilized as functional foods. They contain proteins with high biological value and also bioactive compounds such as phenolic compounds, flavonoids, vitamins, and minerals that can possess positive health effects on the body. Three types of pseudocereals (amaranth, buckwheat, and quinoa) were evaluated for polyphenols and antioxidant activity. Spectrophotometric methods were used for the determination of free phenols amount with Folin-Ciocalteu reagent, and total antioxidant capacity (TAC) with DPPH and ABTS reagents. Free phenols, the predominant part of polyphenols, were in pseudocereals in the range from 12.4 to 678.1 mg GAE.100g-1. The highest content of FP was found in buckwheat products (146.8 ”“ 678.1 mg GAE.100g-1); quinoa and amaranth products reached much lower values (up to 226.1 mg GAE.100g-1). Antioxidant activity was in an agreement with the FP amounts order, the highest TAC values were again for buckwheat products (167.3 ”“ 473.9 and 876.9 ”“ 3524.8 mg TE.100g-1), followed by quinoa (78.2 ”“ 100.6 and 738.9 ”“ 984.5 mg TE.100g-1) and amaranth ones (25.0 ”“ 69.7 and 118.2 ”“ 431.4 mg TE.100g-1). A high positive correlation between FP amount and TAC values was evaluated for analyzed pseudocereals. The highest content of free phenols and the best antioxidant potential showed buckwheat wholemeal flour, so buckwheat could be characterized as a great source of free phenols with high antioxidant activity.

scholarly journals Antioxidant Activity of Fruits Produced in Northern Greece

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1341-1344 ◽  
Author(s):  
Antonios Petridis ◽  
Magdalene Koukourikou ◽  
Thomas Sotiropoulos ◽  
Dimitrios Stylianidis
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
High Activity ◽  
Antioxidant Activities ◽  
Fresh Weight ◽  
Northern Greece ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Cornelian Cherry ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities (μmol ascorbic acid equivalent/g fresh weight) of the edible part of fruits grown in northern Greece were determined using the ferric-reducing antioxidant power (FRAP) assay. Differences were observed among species as well as cultivars of the same species. Cornelian cherry had the highest FRAP value followed by jujube. Cherries, black grapes, and blackberry showed high activity followed by pears, persimmons, plums, peaches, white grapes, pomegranates, apples, nectarines, kiwifruits, quinces, figs, and apricots.

scholarly journals Phytochemical study, polyphenols determination and evaluation of antioxidant activity of Origanum compactum and Satureja calamintha nepeta from the region of Ouazzane (Morocco)

2020 ◽  
Vol 10 (4) ◽  
pp. 396
Author(s):  
Mouhcine Hayani ◽  
Nadia Benhlima ◽  
Amal Bouzoubaa ◽  
Atika Ailli ◽  
Aman Allah Gourich ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Medicinal Plants ◽  
Crude Extract ◽  
Reducing Power ◽  
Phytochemical Study ◽  
Antioxidant Power ◽  
Aromatic And Medicinal Plants ◽  
Origanum Compactum ◽  
High Antioxidant Activity

<p>Among natural remedies, aromatic and medicinal plants constitute an essential source that deserves scientific attention. Thus, our study aims to promote the virtues of two aromatic and medicinal plants found in the wild in the region of Ouazzane (Morocco). The phytochemical tests of these species have shown the presence of flavonoids, gallic and catechic tannins, cyanidin, sterols and triterpenes. However, alkaloids, carotenoids, saponosides and reducing compounds were not detected. Then, extraction of total polyphenols from O<em>riganum compactum</em> and <em>Satureja calamintha nepeta</em> was conducted for 48 hours by maceration with a mixture of methanol/water (80/20) used as a solvent. Extractions yielded 35.2% crude extracts for <em>Origanum</em> <em>compactum</em> and 34.4% for <em>Satureja calamintha</em> <em>nepeta</em>. The fractionation of these crude methanol extracts was performed with three organic solvents of increasing polarity (chloroform, ethyl acetate then, n-butanol). Total phenol content of the extracts and fractions was assessed by the method of Folin-Ciocalteu. This revealed that the phenol-rich fractions were the crude extract for <em>Origanum compactum</em> and the butanol fraction for <em>Satureja calamintha</em> <em>nepeta</em>. Finally, the evaluation of the antioxidant power which was carried out using the method of trapping the free radical DPPH• and the reduction of iron by the method of FRAP. The DPPH• test showed a high antioxidant activity of the samples: 53.1 µg/ml of crude extract of <em>Origanum compactum</em> was needed to reduce 50% of DPPH•. The same IC<sub>50</sub> was obtained for ascorbic acid (standard antioxidant). For <em>Satureja calamintha nepeta,</em> the IC<sub>50</sub> was 67.5 µg/ml. On the other hand, the FRAP test revealed that the methanolic extract of <em>origanum compactum</em> has higher reducing power than that of n-buthanolic extract of <em>satureja calamintha nepeta. Still,</em> it remains relatively low compared to ascorbic acid. The high antioxidant activity of these two species could justify their use in traditional medicine for the treatment of many diseases.</p>

scholarly journals Maintenance of postharvest quality of blackberry (Rubus fructicosus L.) fruits through salicylic acid and CaCl2 immersions

2019 ◽  
Vol 18 (4) ◽  
pp. 121-128
Author(s):  
Ferhan Sabir ◽  
Sevil Ozcelik ◽  
Alper Kucukbasmaci
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Salicylic Acid ◽  
Storage Period ◽  
Titratable Acidity ◽  
Soluble Solids ◽  
Postharvest Quality ◽  
Total Antioxidant ◽  
High Antioxidant Activity

Blackberries (Rubus spp.) have significant nutraceutical properties, providing phenolic compounds for the consumers. These compounds are useful to human health due to their high antioxidant activity. However, most blackberries destined for fresh markets become unmarketable in 2 to 3 days due to decay and leakage, because they have thin and fragile skin. The present study was aimed to investigate the effect of salicylic acid (SA) and calcium chloride (CaCl2) on extending the postharvest quality and bioactive compounds of blackberry during refrigerated storage. Blackberry fruits cv. ‘Jumbo’ were dipped in 2% CaCl2, or in 2.0 mM salicylic acid (SA) for 10 min. Total soluble solids contents, titratable acidity, pH, ascorbic acid contents, total phenolics contents, and total antioxidant activity were investigated initially on 4th, 7th, or 10th day (d) of storage. Changes in fresh weight, titratable acidity, ascorbic acid and total phenol contents were markedly delayed by both treatments. Berries treated with 2 mM SA or 2% CaCl2 exhibited markedly better visual quality during the storage period. Considering the overall findings, this study revealed that postharvest SA or CaCl2 applications maintained the storage-life and conserved the valuable marketing features of blackberries over 10 d in cold storage, presumably due to their inhibitory effects on fruit senescence.

scholarly journals Effects of different heat treatments on the antioxidant activity and ascorbic acid content of bitter melon, Momordica charantia

2019 ◽  
Vol 22 ◽  
Author(s):  
Zhi Xiang Ng ◽  
Umah Rani Kuppusamy
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Acid Content ◽  
Radical Scavenging ◽  
Ascorbic Acid Content ◽  
Heat Treatments ◽  
Total Antioxidant Activity ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power

Abstract This study investigated the effects of different heat treatments, namely boiling, microwaving and pressure cooking, on the reduced ascorbic acid content and antioxidant activity in Momordica charantia extracts. The total antioxidant activity of M. charantia extracts was determined by ferric reducing-antioxidant power and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays. The reduced ascorbic acid content was estimated by ferric reducing-antioxidant power-ascorbic acid assay and confirmed by high performance liquid chromatography. Regardless of the cooking method, all cooked M. charantia extracts had significantly (p < 0.05) higher total antioxidant activity, radical scavenging potency and reduced ascorbic acid content than the uncooked sample. The decreasing order of reduced ascorbic acid in M. charantia was as follows: microwaved > boiled > pressure-cooked > uncooked. The reduced ascorbic acid in M. charantia strongly correlated with the total antioxidant activity (r = 0.958, p < 0.05) and radical scavenging potency (r= 0.951, p < 0.05). The principle component analysis showed that 87% of the variation in the antioxidant values of M. charantia extracts was attributed to the different cooking methods. Heat treatment either by boiling or microwaving for a short period of time (10 minutes) improved the reduced ascorbic acid content in the M. charantia extract. This study showed that the reduced ascorbic acid could be retained under high temperature and pressure in M. charantia extracts.

The Effect of Stress and Warfarin on the Adrenal Gland in Relation to Spontaneous Hemorrhage, as Judged by Measurement of Adrenal Ascorbic Acid and Serum Corticosterone

1971 ◽  
Vol 26 (02) ◽  
pp. 275-288 ◽  
Author(s):  
S Chattopadhyay ◽  
D. D Johnson ◽  
G. J Millar ◽  
L. B Jaques
Keyword(s):  
Ascorbic Acid ◽  
Acid Concentration ◽  
Ascorbic Acid Concentration ◽  
Corticosterone Concentration ◽  
Serum Corticosterone ◽  
Spontaneous Hemorrhage ◽  
Combined Action ◽  
Series Of Experiments ◽  
Pituitary Adrenal Axis

SummaryRats were subjected to the following procedures: No treatment, Stressor (10% NaCl i.p.), Warfarin for 7 days, Stressor followed by Warfarin; and groups were sacrificed at intervals for assessment of spontaneous hemorrhage and of adrenal ascorbic acid concentration. There was no hemorrhage in the no treatment and stressor groups; some hemorrhage in the warfarin group; profound hemorrhage with Warfarin + Stressor. The adrenal ascorbic acid concentration was found to be lower, 8 h and again 5 days after stress, and remained lower in the warfarin + stress animals. Warfarin had no effect on adrenal ascorbic acid level.In another series of experiments in which the stress consisted of an electric current to the cage floor for 6 sec over 15 min, rats were sacrificed daily for determination of serum corticosterone concentration and occurrence of spontaneous hemorrhage. There was a statistically significant increase of serum corticosterone concentration with stress, warfarin and combined warfarin and stress treatments (P< 0.001 for all three variables). There was a significant correlation (r = 0.96 and 0.89, P< 0.01) for serum corticosterone concentration with hemorrhage score and incidence of hemorrhage in stressed rats receiving warfarin, but not in those receiving only warfarin. The results indicate an activation, rather than an exhaustion, of the pituitary-adrenal axis during the combined action of anticoagulant and stress, which results in the development of spontaneous hemorrhage.

Composition and Antioxidant Activity of Aqueous Extracts Obtained from Herb of Tansy (Tanacetum Vulgare L.)

2018 ◽  
Vol 69 (5) ◽  
pp. 1041-1044
Author(s):  
Claudia Anca Mot ◽  
Andreea I. Lupitu ◽  
Simona Bungau ◽  
Ciprian Iovan ◽  
Dana Maria Copolovici ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Veterinary Medicine ◽  
Pharmaceutical Industry ◽  
Phenolic Content ◽  
Food Preservation ◽  
Aqueous Extracts ◽  
Tanacetum Vulgare ◽  
High Antioxidant Activity ◽  
Natural Additives

The aim of this study was to determine the phenolic content and antioxidant activity of extracts obtained from herb of Tanacetum vulgare L. (tansy) harvested in two different locations from the western part of Romania. Aqueous extracts of tansy presented high antioxidant activity that ranged between 295.77 and 653.65 mg GAE/L, and remarkable percentage of inhibition (31.82-65.05 %). The compounds quantified in the plant extracts that were determined by UHPLC are: ascorbic acid, riboflavin, pyrocatechol, rutin, quercetin, and kaempferol. Extracts obtained from tansy flowers showed higher content of phenols compared to that obtained from leaves. Similarly, the flower extracts present a high antioxidant activity that could suggest their use as natural additives in food preservation and also in pharmaceutical industry and veterinary medicine.

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