scholarly journals The Use Of Demineralized Freeze-dried Bovine Bone Xenograft In Reducing Post-surgical Periodontal Recession

2005 ◽  
Vol 12 (1) ◽  
pp. 37-40
Author(s):  
N.A. Baharuddin ◽  
S. Kamin ◽  
A.R. Samsuddin
Keyword(s):  
Bovine Bone ◽  
Freeze Dried ◽  
Periodontal Recession

Porous Titanium Membranes Combined with Various Graft Materials Induce Exophytic Bone Formation in Rabbit Calvaria

2006 ◽  
Vol 309-311 ◽  
pp. 427-432 ◽  
Author(s):  
Y. Kim ◽  
Y.H. Kown ◽  
J.B. Park ◽  
J.H. Chung ◽  
H.N. Lim ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Graft ◽  
Porous Titanium ◽  
Control Group ◽  
Bovine Bone ◽  
Graft Material ◽  
New Bone Formation ◽  
Freeze Dried ◽  
Custom Made ◽  
Human Cancellous Bone

The purpose of this study was to examine if the application of custom-made porous titanium membranes combined with bone graft materials promotes exophytic bone formation in rabbit calvaria. For this purpose, round decorticated calvaria sites were created using a round carbide bur. In the control group, rectangular parallelepiped-shaped porous titanium membranes (RPTMs) were placed on the decorticated sites and fixed with metal pins. In the experimental groups, RPTMs were filled with one of the following bone graft materials prior to fixing with metal pins: bovine bone mineral (BBM), demineralized freeze-dried human cortical bone (DFDB) or freeze-dried human cancellous bone (FDB). Animals were sacrificed at 8 and 12 weeks after surgery, and new bone formation was assessed by histomorphometric as well as statistical analysis. The results indicate that at 8 and 12 weeks, all the experimental groups demonstrated exophytic bone formation. At 12 weeks, DFDB group revealed the most new bone formation (p<0.05) and resorption of grafted materials (p<0.05). On the basis of these findings, we conclude that RPTMs may be used as an augmentation membrane for guided bone regeneration and DFDB as an effective bone-inducing graft material.

scholarly journals Efek Deksametason pada Homeostasis Kalsium dan Ekspresi Transforming Growth Factor Beta-1 Tulang Femur Tikus Wistar Pasca Transplantasi Menggunakan Demineralized Freeze-Dried Bovine Bone Xenograft

2020 ◽  
pp. 98
Author(s):  
Luh Made Sudimantini ◽  
Dhirgo Adji ◽  
Hastari Wuryastuty
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Bovine Bone ◽  
Freeze Dried ◽  
Growth Factor Beta

Penelitian ini bertujuan untuk mempelajari tingkat keamanan penggunaan deksametason sebagai anti radang dan analgetika pada penanganan fraktur tulang femur tikus dengan metode cangkok tulang. Sampel yang digunakan adalah 32 ekor tikus Wistar jantan umur dua bulan dibagi menjadi dua kelompok secara acak, masing-masing kelompok 16 ekor tikus. Kelompok I (kontrol) adalah tikus yang dibuat fraktur dan difiksasi dengan pin intramedular tanpa pemberian bahan cangkokan sedangkan tikus kelompok II adalah tikus yang dibuat fraktur dan difiksasi dengan pin intramedular dan diberikan bahan cangkokan demineralized freeze dried bovine bone xenograft (DFDBBX). Tikus Kelompok I dan II selanjutnya dibagi lagi menjadi dua kelompok, yaitu Kelompok IA, IB, IIA dan IIB masing masing delapan ekor tikus. Tikus A adalah tikus yang tidak diinjeksi dengan deksametason, sedangkan tikus B adalah tikus yang diinjeksi deksametason dengan dosis 0,5 mg/ Kg BB/ hari pasca operasi. Pada akhir minggu I, delapan ekor tikus dari kelompok I (A,B) dan II(A,B) (masing-masing kelompok dua ekor tikus) diambil darahnya untuk analisis total leukosit dan kalsium darah. Sampel urin juga dikoleksi untuk pemeriksaan kalsium urin. Tikus selanjutnya dieuthanasi, tulang femur dikoleksi untuk pemeriksaan histopatologis dan imunohistokimia terhadap ekspresi Transforming Growth Factor-?1 (TGF-. ?1) menggunakan metode Streptavidin-biotin.. Pengambilan sampel berturut-turut selanjutnya dilakukan pada akhir minggu ke-2, 4 dan 8 perlakuan. Data total leukosit, kalsium darah dan urin dianalisis secara statistik menggunakan pola faktorial 2x2x4 sedangkan analisis histopatologis dan imunohistokimia dilakukan secara deskriptif. Hasil analisis statistik pola faktorial dari data konsentrasi kalsium urin, menunjukkan adanya pengaruh yang signifikan dari pemberian deksametason, penggunaan bahan cangkok dan lamanya perlakuan (p<0,05), sedangkan hasil analisis statistik terhadap konsentrasi kalsium darah tidak berbeda nyata (p>0,05). Hasil analisis statistik terhadap total leukosit, menunjukkan bahwa terdapat pengaruh yang signifikan pada pemasangan bahan cangkok (p<0,05). Analisis histopatologis jaringan tulang menunjukkan terjadinya peningkatan infiltrasi sel-sel radang pada kelompok tikus yang tidak diinjeksi deksametason. Sedangkan hasil analisis imunohistokimia menunjukkan ekspresi positif TGF-?1 pada semua kelompok perlakuan. Berdasarkan hasil yang diperoleh, disimpulkan bahwa injeksi deksametason pasca operasi orthopedi dengan maupun tanpa penggunaan Demineralized Fried-Dried Bovine Bone Xenograft (DFDBBX) mampu mengurangi radang, namun dapat mengganggu proses kesembuhan fraktur.

scholarly journals Analyses of Bone Regeneration Capacity of Freeze-Dried Bovine Bone and Combined Deproteinized–Demineralized Bovine Bone Particles in Mandibular Defects: The Potential Application of Biological Forms of Bovine-Bone Filler

2021 ◽  
Author(s):  
David Buntoro Kamadjaja ◽  
Handhito Satriyo ◽  
Aris Setyawan ◽  
Yeni Dian Lesmaya ◽  
Jefry Wahyudi Safril ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Control Group ◽  
P Value ◽  
Bovine Bone ◽  
Regeneration Capacity ◽  
Critical Size Defects ◽  
Freeze Dried ◽  
Significant Difference ◽  
Bone Particles ◽  
Immunohistochemical Analyses

Abstract Objective This study aimed to evaluate bone regeneration capacity of FDBX granules compared to composite DBBM/DFDBX granules for filling of bone defect in rabbit mandible. Material and Methods Critical size defects were created in 45 rabbits' mandible. The defect in the control group is left untreated, while in other groups the defects were filled with FDBX granules and composite DBBM/DFDBX granules, respectively. Specimens were collected at 2, 4, and 8 weeks for histology and immunohistochemical analyses. Significant difference is set at p-value < 0.05. Results The osteoblast-osteoclast quantification, osteoblast expression of Runx2, alkaline phosphatase, collagen-I, and osteocalcin, and osteoclast expression of receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG) in FDBX groups were statistically comparable (p > 0.05) with the composite group, while OPG/RANKL ratio, bone healing scores, and trabecular area were significantly higher (p < 0.05) in the composite compared to FDBX group. Conclusion Composite DBBM/DFDBX granules, within the limitation of this study, has better bone forming capacity than FDBX granules for filling of bone defects in the mandible.

scholarly journals In Vivo Analyses of Osteogenic Activity and Bone Regeneration Capacity of Demineralized Freeze-Dried Bovine Bone Xenograft: A Potential Candidate for Alveolar Bone Fillers

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
David Buntoro Kamadjaja ◽  
Zefry Zainal Abidin ◽  
Riska Diana ◽  
Ikhram Kharis ◽  
Ni Putu Mira Sumarta ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Bone Defects ◽  
Type I ◽  
Bovine Bone ◽  
Potential Candidate ◽  
Micro Ct ◽  
Osteogenic Activity ◽  
Bone Filler ◽  
Freeze Dried ◽  
Significant Difference

Background. Deproteinized bovine bone mineral (DBBM) particle is the commonly used bone graft substitute in implant surgery which is mainly osteoconductive and has very slow degradation. Demineralized freeze-dried bovine bone xenograft (DFDBBX) particle is being developed as a novel xenogeneic bone filler. Objectives. The study aimed to analyze osteogenic activity and bone-forming capacity of DFDBBX particles compared to DBBM particles in alveolar bone defects in rabbit mandibles models. Material and Methods. This study investigated bone defects whether filled with DBBM particles or DFDBBX particles or left unfilled in 30 rabbit mandibles. Specimens were processed for histology, immunohistochemistry, and micro-CT scanning. Statistical difference was set at a p value < 0.05. Results. The quantitative assessment showed a significantly lower number of osteoclasts and a higher number of osteoblasts in the DFDBBX group compared to the DBBM group in 2 and 4 weeks ( p < 0.05 ). Immunostaining analyses showed significantly higher expression of RUNX2, collagen type I, alkaline phosphatase, and osteocalcin in the DFDBBX group compared to the DBBM group in 2 and 4 weeks. Bone healing score in the DFDBBX group was comparable to the DBBM group. Micro-CT presented no significant difference in the volume percentage of the mineralized tissue in the DBBM and DFDBBX groups in spite of the different healing patterns in both groups. Conclusion. DFDBBX particles induced higher osteoblastic activities than DBBM particles at the early stage of healing. Meanwhile, the capacity of bone formation in DFDBBX particles was comparable with DBBM particles at the later stage of healing. Considering the limitation of this study, the results presented DFDBBX particles as potential bone filler candidates.

scholarly journals RANKL expressions in preservation of surgical tooh extraction treated with Moringa (Moringa oleifera) leaf extract and demineralized freeze-dried bovine bone xenograft

2018 ◽  
Vol 50 (3) ◽  
pp. 149
Author(s):  
S. Soekobagiono ◽  
Adrian Alfiandy ◽  
Agus Dahlan
Keyword(s):  
Treatment Group ◽  
Bone Resorption ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Control Group ◽  
Bovine Bone ◽  
Freeze Dried ◽  
Immunohistochemical Techniques ◽  
One Way Anova ◽  
Moringa Leaf Extract

Background: Preservation of sockets is a procedure aimed to reduce bone resorption after tooth extraction. One of the most commonly used xenograft materials is demineralized freeze-dried bone bovine xenograft (DFDBBX). Meanwhile, one of the key regulations in osteoclast genesis process is RANKL bond. A decrease in the number of RANKL expressions can suppress the osteoclast genesis process so that bone resorption can be prevented. The combination of Moringa leaf extract and DFDBBX, as a result, is expected to decrease the number of RANKL. Purpose: This study aimed to measure RANKL expressions in tooth extraction socket treated with Moringa leaf extract combined with DFDBBX. Methods: Fifty six Cavia cobaya rats were divided into eight groups. The first group was a control group with PEG administration onto their extraction sockets. The second group was a treatment group with DFDBBX administration. The third group was a treatment group with Moringa leaf extract administration. The fourth group was a treatment group induced with a combination of DFDBBX and Moringa leaf extract. Examination then was performed on days 7 and 30. After 7 and 30 days, those Cavia cobaya rats were executed and tested with immunohistochemical techniques. Further research data collected then were tested with one-way ANOVA. Results: There were significant differences between the control group and the groups induced with the combination of Moringa leaf extract and DFDBBX. On days 7 and 30, the groups induced with the combination of Moringa leaf extract and DFDBBX had the lowest number of RANKL expressions. Conclusion: The combination of Moringa leaf extract and DFDBBX can decrease the number of RANKL expressions in Cavia cobaya rats on the day 7 and day 30 after tooth extraction.

scholarly journals Biomimetic Composite Scaffold Based on Naturally Derived Biomaterials

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1161
Author(s):  
Ionela Andreea Neacsu ◽  
Adriana Petruta Serban ◽  
Adrian Ionut Nicoara ◽  
Roxana Trusca ◽  
Vladimir Lucian Ene ◽  
...  
Keyword(s):  
Cellular Response ◽  
Composite Scaffold ◽  
Healing Process ◽  
Organic Matrix ◽  
In Vitro Cytotoxicity ◽  
Eggshell Membrane ◽  
Bovine Bone ◽  
Freeze Dried ◽  
Biomimetic Hydroxyapatite

This paper proposes the development of a biomimetic composite based on naturally derived biomaterials. This freeze-dried scaffold contains a microwave-synthesized form of biomimetic hydroxyapatite (HAp), using the interwoven hierarchical structure of eggshell membrane (ESM) as bio-template. The bone regeneration capacity of the scaffold is enhanced with the help of added tricalcium phosphate from bovine Bone ash (BA). With the addition of Gelatin (Gel) and Chitosan (CS) as organic matrix, the obtained composite is characterized by the ability to stimulate the cellular response and might accelerate the bone healing process. Structural characterization of the synthesized HAp (ESM) confirms the presence of both hydroxyapatite and monetite phases, in accordance with the spectroscopy results on the ESM before and after the microwave thermal treatment (the presence of phosphate group). Morphology studies on all individual components and final scaffold, highlight their morphology and porous structure, characteristics that influence the biocompatibility of the scaffold. Porosity, swelling rate and the in vitro cytotoxicity assays performed on amniotic fluid stem cells (AFSC), demonstrate the effective biocompatibility of the obtained materials. The experimental results presented in this paper highlight an original biocomposite scaffold obtained from naturally derived materials, in a nontoxic manner.

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