Robust and Repeatable Biofabrication of Bacteria-Mediated Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process Parameters, and Nanoparticle Size

Bacteria-mediated drug delivery systems comprising nanotherapeutics conjugated onto bacteria synergistically augment the efficacy of both therapeutic modalities in cancer therapy. Nanocarriers preserve therapeutics’ bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune-mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria-nanoparticle (NP) conjugates would carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well-defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems’ efficacious dosage and robust performance. Herein, we utilized our Nanoscale Bacteria-Enabled Autonomous Delivery System (NanoBEADS) platform to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. We also evaluated the effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS. We show that the linkage chemistry impacts NP attachment density while the self-assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS’ growth rate and motility in an NP size-dependent manner. These findings will contribute to the development of scalable and repeatable bacteria-nanoparticle biohybrids for applications in drug delivery and beyond. Corresponding author(s) Email: [email protected]

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Robust and Repeatable Biofabrication of Bacteria-Mediated Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process Parameters, and Nanoparticle Size

10.22541/au.163100509.93917936/v2 ◽

2021 ◽

Author(s):

Ying Zhan ◽

Austin Fergusson ◽

Lacey R. McNally ◽

Richey M. Davis ◽

Bahareh Behkam

Keyword(s):

Drug Delivery ◽

Growth Rate ◽

Process Parameters ◽

Self Assembly ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Dependent Manner ◽

Assembly Process ◽

Antitumor Effects ◽

Therapeutic Modalities

Bacteria-mediated drug delivery systems comprising nanotherapeutics conjugated onto bacteria synergistically augment the efficacy of both therapeutic modalities in cancer therapy. Nanocarriers preserve therapeutics’ bioavailability and reduce systemic toxicity, while bacteria selectively colonize the cancerous tissue, impart intrinsic and immune-mediated antitumor effects, and propel nanotherapeutics interstitially. The optimal bacteria-nanoparticle (NP) conjugates would carry the maximal NP load with minimal motility speed hindrance for effective interstitial distribution. Furthermore, a well-defined and repeatable NP attachment density distribution is crucial to determining these biohybrid systems’ efficacious dosage and robust performance. Herein, we utilized our Nanoscale Bacteria-Enabled Autonomous Delivery System (NanoBEADS) platform to investigate the effects of assembly process parameters of mixing method, volume, and duration on NP attachment density and repeatability. We also evaluated the effect of linkage chemistry and NP size on NP attachment density, viability, growth rate, and motility of NanoBEADS. We show that the linkage chemistry impacts NP attachment density while the self-assembly process parameters affect the repeatability and, to a lesser extent, attachment density. Lastly, the attachment density affects NanoBEADS’ growth rate and motility in an NP size-dependent manner. These findings will contribute to the development of scalable and repeatable bacteria-nanoparticle biohybrids for applications in drug delivery and beyond. Corresponding author(s) Email: [email protected]

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Robust and Repeatable Biofabrication of Bacteria‐Mediated Drug Delivery Systems: Effect of Conjugation Chemistry, Assembly Process Parameters, and Nanoparticle Size

Advanced Intelligent Systems ◽

10.1002/aisy.202100135 ◽

2021 ◽

pp. 2100135

Author(s):

Ying Zhan ◽

Austin Fergusson ◽

Lacey R. McNally ◽

Richey M. Davis ◽

Bahareh Behkam

Keyword(s):

Drug Delivery ◽

Process Parameters ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Nanoparticle Size ◽

Assembly Process ◽

Conjugation Chemistry

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Carbon Nanotubes, Quantum Dots and Dendrimers as Potential Nanodevices for Nanotechnology Drug Delivery Systems

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2013.6.3.2 ◽

2013 ◽

Vol 6 (3) ◽

pp. 2113-2124

Author(s):

Prashant Malik ◽

Neha Gulati ◽

Raj Kaur Malik ◽

Upendra Nagaich

Keyword(s):

Carbon Nanotubes ◽

Drug Delivery ◽

Quantum Dots ◽

Self Assembly ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Atomic Scale ◽

Sustained Drug Delivery ◽

Pharmaceutical Nanotechnology ◽

Conventional Device

Nanotechnology deal with the particle size in nanometers. Nanotechnology is ranging from extensions of conventional device physics to completely new approaches based upon molecular self assembly, from developing new materials with dimensions on the nanoscale to direct control of matter on the atomic scale. In nanotechnology mainly three types of nanodevices are described: carbon nanotubes, quantum dots and dendrimers. It is a recent technique used as small size particles to treat many diseases like cancer, gene therapy and used as diagnostics. Nanotechnology used to formulate targeted, controlled and sustained drug delivery systems. Pharmaceutical nanotechnology embraces applications of nanoscience to pharmacy as nanomaterials and as devices like drug delivery, diagnostic, imaging and biosensor materials. Pharmaceutical nanotechnology has provided more fine tuned diagnosis and focused treatment of disease at a molecular level.

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Polymeric Drug Delivery System Based on Pluronics for Cancer Treatment

Molecules ◽

10.3390/molecules26123610 ◽

2021 ◽

Vol 26 (12) ◽

pp. 3610

Author(s):

Jialin Yu ◽

Huayu Qiu ◽

Shouchun Yin ◽

Hebin Wang ◽

Yang Li

Keyword(s):

Drug Delivery ◽

Self Assembly ◽

Drug Delivery Systems ◽

Tumor Therapy ◽

Chemical Properties ◽

Drug Carriers ◽

Disease Diagnosis ◽

Delivery Systems ◽

Polymeric Drug Delivery ◽

Physical And Chemical

Pluronic polymers (pluronics) are a unique class of synthetic triblock copolymers containing hydrophobic polypropylene oxide (PPO) and hydrophilic polyethylene oxide (PEO) arranged in the PEO-PPO-PEO manner. Due to their excellent biocompatibility and amphiphilic properties, pluronics are an ideal and promising biological material, which is widely used in drug delivery, disease diagnosis, and treatment, among other applications. Through self-assembly or in combination with other materials, pluronics can form nano carriers with different morphologies, representing a kind of multifunctional pharmaceutical excipients. In recent years, the utilization of pluronic-based multi-functional drug carriers in tumor treatment has become widespread, and various responsive drug carriers are designed according to the characteristics of the tumor microenvironment, resulting in major progress in tumor therapy. This review introduces the specific role of pluronic-based polymer drug delivery systems in tumor therapy, focusing on their physical and chemical properties as well as the design aspects of pluronic polymers. Finally, using newer literature reports, this review provides insights into the future potential and challenges posed by different pluronic-based polymer drug delivery systems in tumor therapy.

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Nanoparticulate targeted drug delivery systems - A Review

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i2.2246 ◽

2020 ◽

Vol 11 (2) ◽

pp. 2505-2518

Author(s):

Sindhuja Devaraj ◽

Ganesh GNK

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Delivery System ◽

Self Assembly ◽

Drug Targeting ◽

Drug Delivery Systems ◽

Therapeutic Index ◽

Delivery Systems ◽

Developing System ◽

General Method

Nanoparticulate drug delivery system are the rapidly developing system, and nanoparticles are present in the size range of 1-100nm. Nanoparticles composed of various thermal, electrical, and optical property. Nanoparticles offers the potential advantages over the traditional dosage forms it is ascribable to the properties of nanoparticles. Nanoparticulate drug delivery system ensures the site-specific delivery of a drug(Targeting drug delivery) and aids in improving the efficacy of the new as well as old drugs and has the potential in crossing the various physiological barriers and also improves the therapeutic index of the drugs and increases the patient compliance. The objectives of this review is to classify the nanoparticles based on the different groups, surface properties of nanoparticles, describe the strategies of drug targeting, the necessity of nanoparticles their general method of preparation, different methods used in characterization, self- assembly and mechanism of drug release in a systemic manner. The potential advantages and limitations of various nanoparticulate drug delivery systems are also discussed elaborately.

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Faculty Opinions recommendation of Drug Delivery Systems from Self-Assembly of Dendron-Polymer Conjugates †.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.736815161.793571698 ◽

2020 ◽

Author(s):

Donald A Tomalia

Keyword(s):

Drug Delivery ◽

Self Assembly ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Polymer Conjugates

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Design and self-assembly of albumin nanoclusters as a dynamic-covalent targeting co-delivery and stimuli-responsive controlled release platform

Journal of Materials Chemistry B ◽

10.1039/c8tb01791c ◽

2018 ◽

Vol 6 (42) ◽

pp. 6817-6830 ◽

Cited By ~ 1

Author(s):

Wen Liu ◽

Jian Dai ◽

Wei Xue

Keyword(s):

Drug Delivery ◽

Controlled Release ◽

Tumor Microenvironment ◽

Self Assembly ◽

Drug Delivery Systems ◽

Delivery Systems ◽

Stimuli Responsive ◽

Target Sites

Stimuli-responsive nanomaterial-based drug delivery systems that are able to actively target the tumor microenvironment, enhance intratumoral accumulation and release drugs at target sites are attractive therapeutic platforms at present.

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Nanomedicine Applications in Treatment of Primary Central Nervous System Lymphoma: Current State of the Art

Journal of Biomedical Nanotechnology ◽

10.1166/jbn.2021.3133 ◽

2021 ◽

Vol 17 (8) ◽

pp. 1459-1485

Author(s):

Mengyao Wang ◽

Ying Qu ◽

Danrong Hu ◽

Ting Niu ◽

Zhiyong Qian

Keyword(s):

Drug Delivery ◽

Central Nervous System ◽

Nervous System ◽

Drug Delivery Systems ◽

Central Nervous System Lymphoma ◽

Delivery Systems ◽

Brain Diseases ◽

Current Status ◽

Great Promise ◽

Antitumor Effects

Primary central nervous system lymphoma (PCNSL) is a rare but highly aggressive subtype of extra nodal non-Hodgkin lymphoma (NHL), which is confined in the central nervous system (CNS). Despite recent advancements in treatment options, the overall prognosis of PCNSL remains poor. Among many unfavorable factors affecting efficacy, inadequate drug delivery into the CNS is still the thorniest challenge. Blood-brain barrier (BBB) constitutes a significant impediment, restricting entry of most therapeutics to the brain. Nanotechnology has offered great promise for brain diseases, as various nano-based drug delivery systems (NDDSs) have been developed for delivery of theranostic agents in to the CNS. These drug delivery systems possess significant advantages, including good feasibility, reliable safety profile, excellent BBB penetration and potent antitumor effects. As for treatment of PCNSL, numerous well-developed BBB-crossing nano-based strategies can be applied with proper modifications and improvements. Some exquisitely designed NDDSs specific for PCNSL have shown great potential. In this review, we provide a summary on current status of diagnosis and treatment of PCNSL, followed by an overview of BBB-crossing strategies applied in management of PCNSL, both novel and wellestablished. Finally, challenges and future perspectives in this field are also discussed.

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